Streak Plate Technique for The Isolation of Pure Culture_A Complete Procedure (Microbiology)
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- เผยแพร่เมื่อ 19 ส.ค. 2020
- Streaking is a technique for the isolation into a pure culture of the organisms (mostly bacteria), from a mixed population. The inoculum is streaked over the agar surface in such a way that it “thins out” the bacteria. This video presents you a detailed procedure of Streak Plate Technique.
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I'm a third year laboratory medicine student and I did it for the first time few days ago. It was so interesting!
Thanks. Stay with us
Thanks sir I am studying Microbiology , it helped me alot.
You are welcome. Stay with us
MicroChem's Experiments team, YOU ARE ABSOLUTELY AMAZING!!! Thank you so much for sharing!
Thanks a bunch.
I am a pharmacy student in 2nd year wherein I just went to college for 1 month and again due to lockdown the college is closed but in that 1 month we did the practical of microbiology and I felt it so interesting!!
Again I am watching this video for exam point of view but still it reminds me of what I had done 😄
Thank you for the video!!
Thank you so much
Thank you very much for your clear explanation.
Thank you so much. Stay with us.
I like the video, but I think to wait 5 minutes is a lot of time if you need process many samples. You could cool the loop in the agar.
Yes. You are correct. You don't need to wait for 5 min. Usually loop takes to cool max 1.5 min. You can cool faster by touching/stubbing on the sterile agar surface.
In my workplace, we use disposable inoculating loop.
@@melv1n_official No problem. But it must be sterile
@@melv1n_official same here
Or you can just have extra loops not just one.
It was very useful to me to complete my project....❤❤❤
I am a Hindi board student but I understood this video very well because of easy explanation. Microchem's experiment team your videos is amazing! Thank you so much😍😍😍
Back to my first studying year, I was doing it with less efficiency but know thanks to god I do it with more efficiency with a single burn and my prof learnt us to cool the needle in an empty area in the plate so it is a less time consumer to strike a plate and so interesting !
So precisely shown. Easy to understand and follow the steps.
Thanks
I’m a first year dietitian student and I don’t know why are we studying microbiology. But this video was so informative
Microbiology is very interesting. Anyways, thank you
Food science is strongly related to food microbiology. Knowing at least a little bit is essential
@@dimitrinakashidze8627 You are right
Microbiology is very essential to know about microbes as u know that microbes causes dental plaque and all oral oriented infection🤩🤩 I am microbiology student
@@sahinasapnam1704 thanks for staying with us.
Thanks for the video. Very explanatory
Thank you. Stay with us
Thank you very much for your briefly explanation it is very useful for my tomorrow practical examination
You are most welcome. Stay with us.
very insightful, thanks
Very welcome
Thank you so much...very helpful for my experiment
You are welcome. Stay with us.
Whether there is a need of dipping in the bacterial culture after heating the loop in between intervals of streaking.
I would recommend writing infos on the backside of the agar plate not the the lid to avoid mixing up mistakes 🤷🏼♀️
We write info on the backside. But in this video we wrote on the lid to make it visible to you properly. Anyways, thanks for your suggestions.
Excellent video.
Thank you so much.
Very informative video
Thanks for sharing
Thank you
Thank you for ur clear explanation....it's very useful for me keep it up...💖
Thanks to you too. Stay with us
Thanks for this video iam frst year microbiology thank you sir🙂🙂
Thank you. Stay with us for more videos
Very Informative
Thank you
Very informative video
Thank you
Thank you so much
You are welcome
Tq sir for ur clear explanation
You are welcome
Very helpful for my microbiology class. Also what is the background music, it's very good?
Thank you very much!
helpful!
Thank you.
Hi I just want to ask, for the contamination check of freshly made agar media- what is the time frame for incubation? and what temperature is ideal? thank u
35-37 degree Celsius for 18-24 hours.
Tq so much helpful video
Thank you so much. Stay with us.
Thank you
YOU are welcome
That's really great that's my first vid I watch on this Channel I benefited a lot but I really want to now how we could count on colony counter ?? Because I will have an practical exam about it 🙂 your new follower from Egypt 🇪🇬
Different colony counter have different techniques of colony counting. Follow the manufacturer's instructions of your colony counter device
Very good
Thank you
Very nice thks this is my topic for tomorrow 🥺
Most welcome 😊
thank you
You are welcome
after we strake , it fills the petri dish, not in scratches, is that contamination? Or too much bacteria?
Thanks for the brief explanation 😊.
But sire do you have a video on stool mcs?
Not yet
4th year pharmacy student here, doing 2nd year microbiology lab only now thanks to the pandemic. Its microbiology lab final tomorrow, here to see why last time I didn't get any growth in my media! I probably used the loop when it needed more time to cool, the bacterial cells may have died.
Maybe
As a microbiologist it's literaly the less efficient isolating technique I've ever seen. You could sterilyse only once, and do quarter cross on plates, to isolate FCU on the petri dish, in a single burn.
In our lab work, we also complete streaking by burning the loop for single time. But sometimes single burning is not enough to get the isolated colony, specially for the beginner. Because they draw streaking lines roughly overlapping the lines with one another.
Listen, our aim is to get isolated colonies and I can guarantee you that if you follow this method of streaking, you will must get isolated colonies on any of the streaking lines. So how can you say that it is a less efficient method where it serves our purpose?
Anyways, if you are already expert in streaking, ignore this video. This video is only for the beginner.
Thanks for being with us. Peace
@@MicroChemsExperiments I guess it can be for beginners. Yes I didn't knew before watching it 😂 I always manage to get isolated colonies with single burns 😅
@@MicroChemsExperiments but I meant less efficient because of the many burns needed. When I have to do like 300 petri in a day sometimes, if I lost 20min in loop cooling, I wouldn't be able to do a single day of job in a week 😂😂
@@antoine3933 You are thinking in the professional way.
We also do streaking in single burn at our lab and we also know that the burning loop doesn't need 5 minutes to cool. But we made this video in this way, thinking about the beginners.
Anyway, let it go.
Thanks for commenting here.
@@MicroChemsExperiments
I guess haha
Yes yes ofc, few seconds with some "trash" petri dishes are good too 😉
Yep didn't knew my bad, have a nice evening 😁 👋
Very interesting content, really appreciate your effort,
Please how can l isolate colony from double layer ager plate as VrbL, VrbG and the colony not on surface but in between the layer or inside the layer
Just stub into the semisolid media targeted the bacterial colony with inoculating loop. If you get confident about that the loop touched the target colony a little then take out the loop and streak on another fresh culture plate.
1:17 from where did you get the bacterial culture? How do you made this?
We purchased the reference bacterial ATCC strain and from there we cultured the bacteria in a plate. You can also use any bacterial culture plate for streak plate technique.
We isolate bacteria from various samples in our lab
@@MicroChemsExperiments American type culture collection?
TQ sir nice explanation
Thanks
thanks twin
You are welcome
Goood vedio
Thanks
Thank you very nice from Iraq
Most welcome
Thanks sir 😊
Thanks to you too
Loop should be sterilized by holding it vertically over the flame so the loop and entire length of the wire is red hot at the same time 👍
You are correct.
Please how can one isolate pseudomonas aureginosa from vegetables?? Can this streak method be used? Please it's urgent and I need help for my final year thesis. I have checked everywhere on the net but nothing fruitful
I'm watching this rn bc im gonna do streaking on an agar plate later for my microbio & parasitology class 😌
Okay. All the best
Can u upload glycerol stock preparation for stock pure culture bacteria. I used bile esculin agar for medium for primary and subculture.so what porportion of glycerin stock should i use?
Video is already uploaded
Very gooood information thank uh soo much for it ❤
You are welcome.
Can u make bacterial endospore staining and gram staining.. Video
Yes. We will make
Thank uhh soo much 😊
@@aaryamohite9103 you are welcome
I think your videoes are help for me
Thank you
Thankyou 🤎
You are welcome
I cant understand! What is the purpose of this zig zag streaking pattern! Can u elaborate?
It's my beautiful work 😁😍
Thanks
Why should the inoculation loop not be toohot when taking culture?
Because excessive heat will kill the microorganisms in that colony which is touched by the loop, thus no pure culture will be found in subcultured plate.
thank you very much for the demo! Wondering how to confirm the bacteria at the species level? Do we streak colonies in a new plate based on their morphology?
Culture bacteria into Selective and Differential media first. Then go for the biochemical confirmation. You can also go for the serological test
Or on molecular based
Sir , l am in biotechnology first year and l have this practical in microbiology subject ,can you clear my doubt that T - streak , quadrant method ,hexgonal , simple streaking is these are the method or it just steps that we have to follow while performing this experiment? Please tell me sir.
You have study more. You are the beginner. Search these words in Google and try to understand.
hi, I'm a fifth year in chemistry. I used TSA for soil analyze (sediment). Is there a method to read the colonie result? for example, we got yellow colony, orange colony, white colony. thank you
We will upload video tor Total Bacterial Count soon. Stay with us
@@MicroChemsExperiments Willyou use TSA for total bacterial count?
@@angelinandriamaharo7801 No. I will use Plate Count Agar. But you can also use TSA.
Actually you can streak the plate within 10 - 30 sec after striling the loop as it is made of metals that has the capability to cool faster.
10-30seconds is short for streaking but cooling off a loop can be gaster than 5 minutes
Yes
im a vet nursing student and im watching this as i prepare for my practical assignment xD
Thanks for staying with us.
Thank you sir I am studying microbiology students 1 year
Thank you. Keep watching our videos to learn practical knowledge
Is the fourth quadrant ideal to take an isolated colony? And why so?
Colony density will the higher in first and second quadrant streaking line, thus no isolated colony will be found. But in the third and fourth quarter lines, colony density is much lower & you will find many isolated colonies in pure form. Colony density is decreased from first to fourth quarter lines because of the frequency of burning loop. Burning loop helps to lower the bacterial cell gradually.
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I taked microbiology department sir .This is my first year of microbiology department.
Great!!
It is a wonderful and interesting subject. Stay with us for learning Microbiology practical.
If you noticed that you have no isolated colonies on your streak plate( the streaks are all thicks) why do you think this happened? Thank you.
Its necessary to esterilyse the loop in between smears?
Yes. It is necessary to get isolated colony
Cool😍but its a little bit different from our work in lab for the same test
Okay
Kindly mention the name of this streaking method and also answer require why we are streak in four portion.
It is needed to get isolated and separated pure colony. This method is called: 4 quarter streaking
Please can you tell the colour, size and shape of the growth in the observation method
We will try to develop method for it later
How can we test quality of media prepared specially culture plates?
Go for the growth promotion test using Bioball of reference culture
Plz help me for this also
explain how techniques in marine microbiology can be applied to the isolation of marine bioactive compounds, biomaterial production, production of pharmaceuticals, industrial processes, food production and bioremediation
Noted.
Do I have to wait for 5 minutes if I use a single-use ose?
Don't wait for 5 min. Just wait enough to cool the loop
You have to take new smear eveytime you sterilize the loop right? Like after making the first streak you sterilized it and then again you took the smear from bacterial culture?
No,only first time you need to take smear because he was performing four flame streaking method.
Exactly
Is it okey to open the petridish that much? I mean in what i learn we just open a half of the petridish but, in the result is still have contaminated but you have done it so widely and have single colony too amazing :"). Also, in what i learn when we burn ose, we just wait it not that longer and we burn every quadran. I was afraid that i'm doing wrong. This video is so informative. I will try it later. Thanks.
I am not sure but I think it's okay? since she/he's working on a BSC
Yes. It is okay to keep the petri dish open for the long time if you work inside the Biological Safety Cabinet. Your plate will not be contaminated.
Follow my video properly. You will get isolated colony.
Why nicrome wire is used for inoculated loop
Easy to sterilize by burning and cools rapidly after sterilizing
How much incubation time for testing contamination
24 hours
How much quantity should be taken into the loop??what is the diameter of loop??
You can take a full of a single colony. Or you can just touch the loop on a colony.
Sir , shall we take bacteria from culture only for once time and go on streaking again and again . I mean no need to take bacteria every time we streak?
You are right.
@@MicroChemsExperiments ok tell me one thing more
I have culture a bacteria and pour into a test tube with fixed amount of cells. I want to incubate it once again by adding an inorganic compund into it.
Shall into the same test tube, I should add inorganic compound and keep it in the incubator at appropriate temp overnight?
Es una técnica que aísla bien las colonias, pero en laboratorio no tienes tanto tiempo para practicar esta técnica, cuando te esperan muchas muestras para procesar......
Please write in english
Good morning sir, is this also applicable in isolating endophytic fungi? Thaank you sir 🙏🏽
Good Morning dear.
Yes. You can use this method for fungus culture also.
@@MicroChemsExperiments Thaank you so much for your response sir, More power to you and your channel 🙏🏽❤️
@@jazzopas-iamkajorn5192 So glad to hear that
Do you mean Laminer Air Flow as Biological Safety cabinet
Yes. You are correct
How much time needed to incubate for contamination test
18-24hours
Which is the universal agar?
Tryptone Soya Agar, Nutrient Agar and Plate Count Agar age the universal culture media
Why shouldn't the loop be burned for the third and fourth quadrants??
Low bacterial cell for third and forth.
What questions will the examiners ask on practical exam streak plate techniques
Clear the basic watching this video and take the full concept. Answering to the questions will be easier
Fresh culture incubation time during
24 hours
Hello wanna ask if i burn the loop for third and fourth streak, wont it make any differences? Is it better to burn the loop on 3rd and 4th streak or no?
On the the second streak line, bacterial concentration will be low. So no burning is needed during 3rd & 4th streaking. But you can burn during 3rd streaking.
@@MicroChemsExperiments Thank you so much for replying! I have other question 😅 After burning the loop, do we need to wait exactly 5 min for it to cool down? Or can be shorter maybe around 2 min?
@@Maya-er5xo Maybe 2 min is enough for cooling the loop.
@@Maya-er5xo or you can just get another few extra loops. Burn all the loops first. After it's cool down you can just simply use them without having to burn one loop then wait every 5 mins. Which takes up the time.
@@Maya-er5xo or you can just get another few extra loops. Burn all the loops first. After it's cool down you can just simply use them without having to burn one loop then wait every 5 mins. Which takes up the time.
So how much time exactly
If you are an expert then you can do this test by 2 min only
If we are streak in 4 portion for decreasing microbial load. But why? We are already streak on specific culture medium.
A single bacterial colony have millions of bacterial cell. At the time of streaking, smear contains maximum cell number. First quarter portion will contain lower cell than smear. Second & third quarter portion will contain lower cell number than first quarter. The fourth quarter portion contains lowest cell number. Bacterial cell is decreased gradually from first to fourth quarter.
Decreasing of microbial cell is necessary to get isolated colony in most pure form
@@MicroChemsExperiments sir thank you for replying but my second question is why we are decrease colony?
@@MicroChemsExperiments thanks
@@minhasanbar1854 please knock in our Facebook page for more discussion
how many times we need to deep the inocolum loop in the bacterial media??only once! i don’t understand this😣as you again&again burning the loop& after cooling you are doing zigzag but without deeping into the bacterial media but you did deep before zigzag at 1st attempt😣
We need to touch the bacterial colony only once at the very first time. For taking bacteria. Then we don't need to dip again
In my laboratory I used plastic sterile for rapidly work 👍👍
Great 👍
tell me one thing more
I have culture a bacteria and pour into a test tube with fixed amount of cells. I want to incubate it once again by adding an inorganic compund into it.
Shall into the same test tube, I should add inorganic compound and keep it in the incubator at appropriate temp overnight
Yes. Add into the same tube
@@MicroChemsExperiments
Two questions:
1. Is inorganic or organic compound effect the growth of bacteria.
2. When we add organic or inorganic compound in to.i think there is need to add nutrient media also so that the bacteria can grow well.please guide me.
3. If we take cells from these tubes and put with fixed amount to other four tubes along with inorganic or organic compound.
Shall we add nutrient media again
Please reply me.
Sir could you please tell me if Microbiology is required for working in micro laboratory????
Yes, obviously. For microbiological analysis you always need to work in a clean microbiological laboratory.
Sir I am done my bsc Microbiology, could I allgible for working in Microbiology laboratory??
@@worldofshubhi2918 Obviously. Why not?? Come on!! You are a Microbiologist. Apply to your suitable job. I hope you will do better in Lab work. All the best
Thank you sir, it's helpful 😊
@@worldofshubhi2918 You are welcome. Stay with us.
Which organisms there use
Coliform
Sir I have completed bsc microbiology with biochemistry in 2003-2005 year, now is there any possibility to get job on it plz tell me sir
Apply in different lab
Iam a third year medical laboratory student ( semi final ) and till now my streaking is so bad can you help me with that and tell me how I am going to solve this problem ? 😊😊
It's a practical issue. I can't solve it at this moment. Try to follow my video strictly
Why streak plate is not useful in quantitative estimation?
Because you can't count all of the colonies on the plate and many colonies are burnt during burning the inoculating loop
@@MicroChemsExperiments Wooh! Thank you! That's helps a lot
Can you transleted the viedo to franch because i stadyin in franch please
Do you want subtitle in French?
@@MicroChemsExperiments yes please because I am biologiste and i want learing more about biology
@@youssefaidaoui6157 I will try. Please wait
@@MicroChemsExperiments take your time and thank you brother
@@youssefaidaoui6157 no worries. I will let you know.
👍👍👍
Thanks
How to convert cfu/g to cfu/ml
These two units are same. CFU/g is used for solid sample and CFU/ml is used for liquid sample