Four Quadrant Streak procedure - How to properly streak a Petri plate for isolated colonies

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  • เผยแพร่เมื่อ 26 พ.ย. 2024

ความคิดเห็น • 119

  • @Ms1101997
    @Ms1101997 2 ปีที่แล้ว +269

    I’m a new microbiology student and I’ll do this for my tomorrow’s lab 😅 I was confused but thanks god I found this video

    • @HardyDx
      @HardyDx  2 ปีที่แล้ว +20

      We love helping new micro enthusiasts! Feel free to browse our other tutorials for your class too!

    • @winispecial
      @winispecial 2 ปีที่แล้ว +5

      "Thank God you found this video "

    • @CrankyRayy
      @CrankyRayy ปีที่แล้ว

      @@winispecial " " Thank God you found this video " "

    • @mdraihan1582
      @mdraihan1582 8 หลายเดือนก่อน

      What's your country name?

    • @Ms1101997
      @Ms1101997 8 หลายเดือนก่อน

      @@mdraihan1582 I’m from the US, California

  • @notjeb_9409
    @notjeb_9409 ปีที่แล้ว +39

    its so wild how a lot of ppl commenting here r college students or professionals n here i am watching this for our lab activity in my high school biology class. cool video, helps a lot

  • @daliadivicpuskarski7453
    @daliadivicpuskarski7453 2 ปีที่แล้ว +31

    Yesteday I streaked a Petri dish for the first time in my life. I've watched this video before doing it and it really helped me 🧫 Thank you 😊

    • @HardyDx
      @HardyDx  2 ปีที่แล้ว +5

      That's wonderful. Glad it helped!

  • @clairem290
    @clairem290 2 ปีที่แล้ว +13

    I'm a college instructor and I'm sending this video to my students before they do their first-ever cultures!

    • @HardyDx
      @HardyDx  2 ปีที่แล้ว +4

      We love our instructors and our students! Feel free to reach out to us should you need materials for your students as well!

    • @Flowers12660
      @Flowers12660 9 หลายเดือนก่อน +1

      Well as an instructor why don't you show your students this yourself? Isn't this supposed to be your job to teach? It would be alot better. I really dislike and hate how college so called instructors and lecturers are relying so much on youtube videos instead of doing the work themselves.

    • @clairem290
      @clairem290 9 หลายเดือนก่อน +1

      I did streak some plates myself, which came in handy later. However, a nice TH-cam video they could watch the night before was also really helpful. To make up for your previous instructors who didn't explicitly show you enough things, I will now show you how to spell the phrases "so-called" and "a lot."

  • @Faceless_Chat
    @Faceless_Chat 7 หลายเดือนก่อน +4

    Very helpful. I have a "unknown bacterium" assignment that starts today. This really helped bc I already forgot you have to sterilize the loop between streaking...

  • @sloane.2747
    @sloane.2747 ปีที่แล้ว +16

    This was so much fun when I did it, microbiology was my literal favorite class my first semester of college

    • @HardyDx
      @HardyDx  ปีที่แล้ว +2

      Glad to hear it! Keep on learning!

  • @lorettarodman9117
    @lorettarodman9117 4 หลายเดือนก่อน +1

    I was taught a very different way, always remember that there is more than one way to streak plates everyone! Find what works best for you. Also if you have a bacteria that really sucks at growing on plates from freezer stock I’ve found that not flaming between streaks helps with that

  • @nina-ge6mn
    @nina-ge6mn 2 ปีที่แล้ว +19

    I love microbiology and honestly its just so satisfying to watch people streak plate
    I'm doing a microbiology project that involves e. Coli K-12 plating and im so excited

    • @HardyDx
      @HardyDx  2 ปีที่แล้ว +5

      Nina, we love projects for the tiny humans! Please feel free to reach out to your local sales rep for product, we'd love to help out the next generation of micro-microbiologists!

  • @DanTheisen
    @DanTheisen 2 ปีที่แล้ว +32

    This is an excellent video. Thanks, Hardy for showing us how this is properly done. Much more to it that a squiggle of bugs on a petri plate!

    • @HardyDx
      @HardyDx  2 ปีที่แล้ว +2

      Glad it helped!

    • @DanTheisen
      @DanTheisen 2 ปีที่แล้ว +1

      As required by law, I’m disclosing that I was employed by Hardy Diagnostics at the time of this comment.

    • @Sean-ur4cb
      @Sean-ur4cb 2 ปีที่แล้ว +1

      @@DanTheisen what did you sign that told you to disclose this info?

    • @DanTheisen
      @DanTheisen 2 ปีที่แล้ว +2

      I don’t recall where I saw it but this is, as far as I know, meant to prevent large companies from having their employees promote their products as if they are customers. I have not signed anything from the company compelling either a comment or a disclaimer about the comment. The law as I read it made sense to me from a consumer protection point of view, which is why I happily added it.

    • @Sean-ur4cb
      @Sean-ur4cb 2 ปีที่แล้ว +1

      @@DanTheisen ahh I see, thanks for the reply. Have a good day!

  • @johnson1262
    @johnson1262 2 ปีที่แล้ว +5

    My universities professor , didn’t tech to put the petri disk , up side down when going into incubator. Thank you for sharing.

    • @HardyDx
      @HardyDx  2 ปีที่แล้ว +6

      Oh no! When media is incubated "upside down" like that, with the media facing upwards, condensation can accumulate on the face of the agar and inhibit proper growth and developing colonies. If you invert your plates, you'll definitely have better-looking plates than the rest of your class!

  • @AL_ayyubi1137
    @AL_ayyubi1137 ปีที่แล้ว +4

    I'm medical laboratory technician(MLT) and 11 lecture is included for simple technique in tomorrow thank you for this video
    I appreciate it 🤩

    • @HardyDx
      @HardyDx  ปีที่แล้ว +4

      You are welcome, I am glad the video was helpful. Keep up the great work as a MLT. You are important.

  • @Cyan-J-e5u
    @Cyan-J-e5u 9 หลายเดือนก่อน +1

    It's self explanatory and the visuals, helps for easy remembrance.
    Thanks.

    • @HardyDx
      @HardyDx  9 หลายเดือนก่อน

      You are very welcome. I am glad our video was useful for you.

  • @ry_n007
    @ry_n007 6 หลายเดือนก่อน +1

    thank you for saving my ee broski 🙏🙏

  • @Dananq98
    @Dananq98 2 ปีที่แล้ว +14

    So useful description, thanks a lot 👏🏻

    • @HardyDx
      @HardyDx  2 ปีที่แล้ว

      Glad it was helpful!

  • @ayesha-it5yw
    @ayesha-it5yw 2 ปีที่แล้ว +4

    I learnt this method today in my microbiology lab.

    • @HardyDx
      @HardyDx  ปีที่แล้ว

      That's great Ayesha! Keep up the proper four quadrant streak method, you will be a pro in no time!

  • @leannajulien4175
    @leannajulien4175 2 ปีที่แล้ว +5

    I didn't know I have place it in the heat everything I learned something new. Thanks

    • @HardyDx
      @HardyDx  2 ปีที่แล้ว

      We're always here to help you learn! We have a great Technical Services team should you ever have questions!

  • @tayyabirshad3029
    @tayyabirshad3029 ปีที่แล้ว +2

    Today may microbiology practical exam it's help alot!!🥰🥰🥰🥰

    • @HardyDx
      @HardyDx  ปีที่แล้ว +1

      Glad to hear the video was useful. You are very welcome. Good luck with your exam. You will do great!

  • @anycamenzind
    @anycamenzind ปีที่แล้ว +1

    Will there be a video available that talks about the microscope and Khoeler illumination? Unfortunately, part of the practical microbiology and laboratory sessions were taken in times of pandemic. Likewise, I would greatly appreciate if you could explain how the UFC colony counter works. Your explanations are great!
    Thank you 🙌🙌🙌

  • @Manthab
    @Manthab 2 ปีที่แล้ว +1

    Don’t know how I got here at 1am but it was fascinating and informative if I ever need to know this I’m covered😁

    • @HardyDx
      @HardyDx  ปีที่แล้ว +2

      Welcome down the rabbit hole of microbiology, Mantha! We've got a whole channel of videos if you ever need more obscure information to get you even more hooked 😃!

  • @ruchika_899
    @ruchika_899 ปีที่แล้ว +2

    Gorgeous explanation 💥❤️

    • @HardyDx
      @HardyDx  ปีที่แล้ว +1

      Thank you, glad you liked it!

  • @apokalypthoapokalypsys9573
    @apokalypthoapokalypsys9573 2 ปีที่แล้ว +2

    I'm in an IT MSc but this is perfect ASMR

    • @HardyDx
      @HardyDx  2 ปีที่แล้ว +1

      Love it! That's a whole new industry we should break into!

  • @nangsanbhalangblah333
    @nangsanbhalangblah333 2 ปีที่แล้ว +3

    Great vid, I've a question though. Why is the media red in color? Is there a different type of agar used?

    • @918mounika7
      @918mounika7 2 ปีที่แล้ว +2

      It's probably blood agar medium, blood added to regular nutrient agar medium

    • @HardyDx
      @HardyDx  2 ปีที่แล้ว +3

      Nangsanbhalang Blah, Great question about the coloration of the agar! This was streaked on our Blood Agar, an all-purpose growth agar with hemolytic properties. Some organisms will hemolyze the blood in the agar, leaving clear zones around the colonies, something we won't see on traditional TSA. Hope that helps!

  • @ruchika_899
    @ruchika_899 ปีที่แล้ว +1

    Excellent explanation ❤

    • @HardyDx
      @HardyDx  ปีที่แล้ว +1

      I am glad our video was useful for to you.

  • @AliyuNanaaishat
    @AliyuNanaaishat 7 หลายเดือนก่อน

    Please can I see a video that will teach me more on how to carry out indoor air quality using SDA and PDA

  • @KHALILLABSKILLS
    @KHALILLABSKILLS ปีที่แล้ว +1

    It's absolutely Amazing sir.

    • @HardyDx
      @HardyDx  ปีที่แล้ว

      Thanks, it's our pleasure.

  • @1alex92
    @1alex92 2 ปีที่แล้ว +5

    Wish I seen this 2 weeks ago when I had a test!
    ...nevertheless.. I just subscribed ☺️

    • @HardyDx
      @HardyDx  2 ปีที่แล้ว +1

      Welcome to the online Hardy family! Stay tuned for more great content soon to come!

  • @KHALILLABSKILLS
    @KHALILLABSKILLS ปีที่แล้ว +1

    I really appreciate you sir.

  • @jennisagala1263
    @jennisagala1263 ปีที่แล้ว +1

    thanks a lot, i hope, my thesis will be finisih

  • @JoshAngelo-k7b
    @JoshAngelo-k7b ปีที่แล้ว

    How do you report colony growth on a 4 way streaking? along with its size, shape, transparency, consistency, and color.. Thanks

  • @jessical4866
    @jessical4866 2 ปีที่แล้ว +14

    I’m not a teacher or a student… why am I so fascinated with this? 😂

    • @HardyDx
      @HardyDx  2 ปีที่แล้ว +10

      I mean we can't blame you, we do this for a living. Our whole lives are this, welcome to the obsession!

    • @GaneshKunayak
      @GaneshKunayak ปีที่แล้ว

      Who are you 🤣😂

    • @affiliatemarketinginnigeri7617
      @affiliatemarketinginnigeri7617 ปีที่แล้ว

      I'm a passer-by as well and I am entranced ❤️

  • @YusmaArfanDogar
    @YusmaArfanDogar หลายเดือนก่อน

    I'm student of DPT and tomorrow I have to perform this practical

  • @JesusLightsYourPath
    @JesusLightsYourPath หลายเดือนก่อน

    In my class they never told us to reheat the probe before creating the next spread section. Is that why our colonies are always too big? Or is it possible to make small colonies without burning the probe in between each swab?

    • @HardyDx
      @HardyDx  หลายเดือนก่อน

      @JesusLightsYourPath Great question. The reason that the metal loop is sterilized for each quadrant is to eliminate extraneous 'crossover' of organism when you streak each quadrant out. By the final streak, the number of cells on the loop should be diluted and low enough that single colonies are dispersed and isolated on the agar surface. If you were to not sterilize between streaks you would end up with a much more confluent 'lawn' of bacteria because of all the extra organism on the loop when you go to streak your successive quadrants.
      To learn more about how to properly streak a Petri plate visit our blog. hardydiagnostics.com/blog/petri-plate-2023

  • @KHALILLABSKILLS
    @KHALILLABSKILLS ปีที่แล้ว +1

    It's well explained.wow

  • @nithinmuluka7987
    @nithinmuluka7987 2 ปีที่แล้ว +2

    Thank you sir very nice video... Pls upload more videos

    • @HardyDx
      @HardyDx  2 ปีที่แล้ว

      Yes, stay tuned. More video are on the way!

  • @todddillerson4053
    @todddillerson4053 2 ปีที่แล้ว +6

    I do this every day for the hospital I work at :)

    • @HardyDx
      @HardyDx  2 ปีที่แล้ว +1

      That's great! Heroes wear lab coats not capes. Which hospital do you work at?

    • @Hellamoody
      @Hellamoody 2 ปีที่แล้ว +1

      @@HardyDx boundaries Dwight!

  • @abdullahmohammad6803
    @abdullahmohammad6803 12 วันที่ผ่านมา

    I did it last week, applied so much force that it tore the agar . I’m worried I might do bad for the practical.

  • @vennelagovindugari5420
    @vennelagovindugari5420 2 ปีที่แล้ว +5

    Perfect!!

  • @mohanzm12
    @mohanzm12 2 ปีที่แล้ว +3

    What do we call the thing she used to flame the loop ? 😅

    • @DadaClaire
      @DadaClaire 2 ปีที่แล้ว +1

      I'm curious too. I'm working with unicellular fungi and bacteria since time, streaking them to isolate but it's the first time ever I'm seeing that instrument.

    • @vanessadang4674
      @vanessadang4674 2 ปีที่แล้ว +2

      It is called a bacticinerator :)

    • @DadaClaire
      @DadaClaire 2 ปีที่แล้ว +1

      @@vanessadang4674 Thank you!

    • @HardyDx
      @HardyDx  2 ปีที่แล้ว +1

      @Mohan The tube the loop was inserted into is a special type of sterilizer. Placing non-sterile metal loops into this tube for a few seconds will sterilize these loops so you can re-use these again and again. Hope that answers your questions!

    • @mohanzm12
      @mohanzm12 2 ปีที่แล้ว +1

      @@vanessadang4674 wow .thank you

  • @Szaisthatyou
    @Szaisthatyou 2 ปีที่แล้ว +1

    I love my major ❤

  • @RaiSaysS
    @RaiSaysS 2 ปีที่แล้ว +5

    Thank you

    • @HardyDx
      @HardyDx  2 ปีที่แล้ว

      You're welcome.

  • @joannadevung4358
    @joannadevung4358 7 หลายเดือนก่อน

    If i accidentally tore the surface of the agar using the loop during streaking, will the bacteria colonies still able to grow?

    • @HardyDx
      @HardyDx  6 หลายเดือนก่อน +1

      Colonies will still grow, yes. However, it is usually best to restart on a fresh plate as the puncture could lead to difficulties in reading/counting colonies or could cause unwanted areas of heavy growth. Don't worry though! Marring the surface is extremely common, especially in beginners. Keep practicing and you'll be able to do it as if it were nothing!

  • @ceciliaovansa2738
    @ceciliaovansa2738 9 หลายเดือนก่อน +1

    Nice one

    • @HardyDx
      @HardyDx  9 หลายเดือนก่อน

      Thanks for watching

  • @SaidaZaknoune
    @SaidaZaknoune ปีที่แล้ว

    Quand utiliser la methode en stries?

  • @sunny4483
    @sunny4483 ปีที่แล้ว

    Can we not streak circular on plate instead of streaking zig zag? I'm curious

    • @MrDubbindan
      @MrDubbindan ปีที่แล้ว

      There is an instrument that does streak with a plastic "comb" after pipetting a volume on a plate and it does make circles ... The Previ Isola

  • @hebahariri9936
    @hebahariri9936 ปีที่แล้ว +1

    Hello, I'm a microbiologist and a content creator on Instagram educating microbiologists in Arabic, can I translate your video and post it on my account? with sharing your video link of course. thank you.

    • @HardyDx
      @HardyDx  ปีที่แล้ว

      Yes, that would be fine as long you tag and credit Hardy Diagnostics in your video description and link it back to the original video. Keep up the great work in microbiology.

  • @GaneshKunayak
    @GaneshKunayak ปีที่แล้ว

    Bdw i think this must be carried out inside a laminar air flow

  • @pastmonster446
    @pastmonster446 2 ปีที่แล้ว +1

    Watching this after I took my lab practical exam, and I definitely fucked it up

  • @akritikuikel8506
    @akritikuikel8506 ปีที่แล้ว

    how to get a job or internship of microbiology in europe. I have done bachelor in microbiology and working in a restaurant since 4 years. Did not even get any inernship. Already forgot all the techniques and skills in these 4 years . sad sad

    • @BlueFox61
      @BlueFox61 7 หลายเดือนก่อน

      Build a portfolio of your interests and maybe your thesis paper too. It's hard to get an internship now

  • @lilianmumbo880
    @lilianmumbo880 2 ปีที่แล้ว +3

    5 method of preparing specimen

  • @chrisk6469
    @chrisk6469 ปีที่แล้ว +1

    Watching them tear up the agar like that was a bit painful ngl

  • @M313_1
    @M313_1 9 หลายเดือนก่อน

    *ثانكيو* 🦦

  • @mtado4933
    @mtado4933 6 หลายเดือนก่อน

    yeah i fucked it up so badly

  • @عبدالرحمنحسن-و6ث
    @عبدالرحمنحسن-و6ث 2 ปีที่แล้ว +1

    عربي وين

    • @Behejrbrbrjrj
      @Behejrbrbrjrj ปีที่แล้ว +1

      هنا موجودين 🙂🙂

  • @kasisfile
    @kasisfile ปีที่แล้ว

    😮

  • @SOPHIA-yf7ws
    @SOPHIA-yf7ws ปีที่แล้ว

    A++++

  • @alvinxyz7419
    @alvinxyz7419 ปีที่แล้ว

    I thought the title said steak

  • @sriningsih4808
    @sriningsih4808 2 ปีที่แล้ว

    kangen haha

  • @John-fr6wo
    @John-fr6wo ปีที่แล้ว +10

    For God so loved the world that He gave His one & only Son, that whoever believes in him shall not perish but have eternal life. For God did not send His Son into the world to condemn the world, But to save the world through Him. (John 3:16-17)✝️

  • @brystopczynski1295
    @brystopczynski1295 2 หลายเดือนก่อน

    Wha??? She's literally doing like 10-20 passes AS THE NARRATOR IS SAYING "no more than 2-3" or "1-2"!? 🫴🫴?!
    Is her technique fine? Sure. Is it the best? No. But what a terrible example 😠 at least be consistent when making an instructional video.

    • @HardyDx
      @HardyDx  2 หลายเดือนก่อน

      @brystopczynski1295 Thank you for your comment. Yes, this is the correct technique used by microbiologist. When streaking a plate the goal is to reduce the number of cells to isolated colonies from quadrant to quadrant. So when the narrator says overlap no more than 2-3 times, it means only overlap from quadrant #1 to quadrant #2, 2-3 times, then continue to streak the remainder of the quadrant. Each time you go to a new quadrant only overlap your streak 2-3 times, then finish out the streak with the 10 -20 passes shown in the video. Your feedback is heard, and we will continue to improve our videos. We are committed to providing a Culture of Service. If you have any other further questions don't hesitate to reach out. Additional if you would like to know more about the four quadrant streak feel free to visit our blog to learn more about the process. hardydiagnostics.com/blog/petri-plate-2023

  • @FarzadSalim
    @FarzadSalim 2 ปีที่แล้ว +1

    thank you

    • @HardyDx
      @HardyDx  ปีที่แล้ว

      You're welcome.