Microbiology: Urine Culture Primary Set-Up Inoculation and Colony Count

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  • เผยแพร่เมื่อ 9 ม.ค. 2025

ความคิดเห็น • 103

  • @dank9261
    @dank9261 2 ปีที่แล้ว +17

    You are supposed to draw the loop out of the urine in a way the reduces overcharging the loop (seen as bulging of urine out of the loop) and to make sure the loop contains no bubbles before streaking and then redip the loop the same way after streaking the blood agar to inoculate the MacConkie agar. otherwise, you’ll have two extremely different volumes of urine used on the two plates.
    Also, avoid touching the edge of the media where it contacts the plastic plate.

    • @morrisjones9537
      @morrisjones9537 8 หลายเดือนก่อน +1

      I was thinking the same thing as I watched her streak the plate with the same loop. Even though the loop touched a urine plate, I would have used a new loop.

  • @erickapelaez7131
    @erickapelaez7131 3 ปีที่แล้ว +7

    Thank you so much for the videos! I’m glad after taking microbiology lab; it makes so much sense with the videos you are showing! I’m learning a lot and feel prepared by you since this is what my future consists of in this field!!

  • @bisterkding9249
    @bisterkding9249 2 ปีที่แล้ว +12

    Even if the colony count is typically taken from the BAP, it's better practice to re-immerse your calibrated loop with every successive plate you streak.

  • @ericyother1512
    @ericyother1512 2 ปีที่แล้ว +3

    Happy Lab Week! Thanks for the video!

  • @iqraismail5712
    @iqraismail5712 3 ปีที่แล้ว +4

    I am from pakistan and student of bs microbiology. I am too much confused but your vidoes are informative and learn lots of things from them

  • @DarkSamael55
    @DarkSamael55 3 ปีที่แล้ว +1

    I love your streaking skills!

  • @MWOA_
    @MWOA_ 2 ปีที่แล้ว +1

    never use a calibrated loop method before during uni (i studied microbiology but we never use human specimen) so i never knew it's existence until i work for a polytechnic of health. a student went to me and asked about how to interpret the result and there i was, never knew that something like this even exist 🤣🤣🤣
    thank you for the video, this really help me visual the result

  • @yrojas9718
    @yrojas9718 หลายเดือนก่อน

    Well I think the streaking is not the typical you see in the clinical lab or shown on school in my case but is very informative. I have encountered lots of different techniques

  • @healthknowledgeexplorer
    @healthknowledgeexplorer 2 ปีที่แล้ว

    Thank you so much for this educative video Ma'am

  • @dank9261
    @dank9261 2 ปีที่แล้ว +3

    Draw the edge (not face) of the loop against the cup when coming out of the urine (and away from any bubbles). This helps assure correct loading and reduces bubbles in the loop. Kind of like drawing a urine dipstick along the edge of the cup to help reduce overloading the various pads.

  • @irfanullah-uk3ks
    @irfanullah-uk3ks 2 ปีที่แล้ว

    Thnkeo thnkeo ma'am,for your efforts ,,,May God bless you ,and may you get success in evey field of life ,,

  • @edengetaneh27
    @edengetaneh27 7 ชั่วโมงที่ผ่านมา

    This a very informative video but it overlooks that BAP as primary culture media is inappropriate; presence of Proteus complicates colony counting. Instead, CLED agar should be used.

  • @mysteryj7590
    @mysteryj7590 3 ปีที่แล้ว +2

    I love the Microbiology videos!!! I'm definitely going to recommend these for my new techs. Will you please do one on gram stain interpretation?

    • @MedicalLabLadyGill
      @MedicalLabLadyGill  3 ปีที่แล้ว +2

      Thank you for your support! We are not in Micro this semester but I will do my best to get one done when we are.

    • @sushilbarua8862
      @sushilbarua8862 3 ปีที่แล้ว

      @@MedicalLabLadyGill hello how are you?

    • @sushilbarua8862
      @sushilbarua8862 3 ปีที่แล้ว

      @@MedicalLabLadyGill see tobbaco mosaic virus leaf disease and coronavirus in microbiological microscope electronic because it

    • @sushilbarua8862
      @sushilbarua8862 3 ปีที่แล้ว

      @@MedicalLabLadyGill search in you tube about tobbac mosaic virus leaf disease and coronavirus in

    • @sushilbarua8862
      @sushilbarua8862 3 ปีที่แล้ว

      Tobacco leaf disease

  • @lachlank.8270
    @lachlank.8270 3 ปีที่แล้ว +2

    Not worried about Proteus swarming all over the BA?

  • @mayzingasmr3145
    @mayzingasmr3145 3 ปีที่แล้ว +1

    Very informative videos. Keep doing this educational videos. Love them. Thank you very much 💖

    • @MedicalLabLadyGill
      @MedicalLabLadyGill  3 ปีที่แล้ว

      Thank you so much for your support! I'm glad you find them helpful! ♡

  • @fgzy123
    @fgzy123 ปีที่แล้ว +1

    Aren't you supposed to avoid spreading the specimen to the outer perimeter? Your loop keeps touching the plastic perimeter. The outer perimeter is where airborne contaminants is most likely to be located.

  • @Struggle..always
    @Struggle..always ปีที่แล้ว

    Good work thanks ❤❤

  • @funny11744
    @funny11744 11 หลายเดือนก่อน

    What is the composition of the media for this test ? Agar and what other components ? thanks

  • @AbdikaniAmarre
    @AbdikaniAmarre ปีที่แล้ว

    which practical microbiology book will you recommend me to read please? I highly appreciate your valuable video.

  • @BeyuiteVictory
    @BeyuiteVictory 19 วันที่ผ่านมา

    I would love to work my internship with you 😷🙏🏻

  • @Sam-rj6kd
    @Sam-rj6kd 3 ปีที่แล้ว +4

    im really thankful to you for making those videos 🙏🏻. i only have one question. im really confused on which type of media we use for each sample (like for urine, we use mac and blood and stool we use selenite... etc). do u have any guide/book that can help me memorize which media to use for each sample? i would appreciate it

    • @MedicalLabLadyGill
      @MedicalLabLadyGill  2 ปีที่แล้ว +2

      Thank you for your support! Not currently. I do have many projects in the works, this being one of them. The main thing to remember is that you're using media that will isolate common pathogens from that body site (specimen). Blood Agar Plate should grow most aerobes and facultative anaerobes. Chocolate will grow fastidious bacteria, such as Haemophilus and Neisseria, but most Haemophilus only grow on CHOC. MacConkey will grow the Gram Negative bacilli such as the Family Enterobacteriaecae (from the GI tract) and Non-fermenters (that get mistaken for Enterobacteriaecae). The anaerobic set-up would be used anytime anaerobes are expected to be the pathogen (aspirates) and should be plated along with the full aerobic primary set-up (BAP, CHOC, MAC).

    • @leggeisreal1553
      @leggeisreal1553 2 ปีที่แล้ว

      @@MedicalLabLadyGill pp 0pol look plol00plloooo9o9ooo

    • @leggeisreal1553
      @leggeisreal1553 2 ปีที่แล้ว

      @@MedicalLabLadyGill 9œlll9ooollo9

  • @mirzasikandar1680
    @mirzasikandar1680 2 ปีที่แล้ว +1

    Why u can't Streak on CLED agar bc I always streak urine on CLED only.....when in student life

  • @aprilsmith8924
    @aprilsmith8924 3 ปีที่แล้ว +28

    This is the problem with cultures. Multiple bacteria is considered to be contamination. Please teach your students the correct info - multi species infections are very much NOT contamination and labeling it as that on cultures prevents women from getting proper treatment. Luckily, I found a urologist who gets this (very few do) and finally identified the 3 bacteria in my infection using a much better test called Pathnostics.

    • @WorldAffairsभारत1
      @WorldAffairsभारत1 2 ปีที่แล้ว +1

      How r u please
      I face klebssiella penumonia bacteria what I do

    • @aprilsmith8924
      @aprilsmith8924 2 ปีที่แล้ว

      @@WorldAffairsभारत1 Please find a good doctor that will treat you with at least 14 days of antibiotics targeted to your infection.

    • @WorldAffairsभारत1
      @WorldAffairsभारत1 2 ปีที่แล้ว

      @@aprilsmith8924 yes bro I treat this problem antibiotics.
      Thanks for suggesting

    • @WorldAffairsभारत1
      @WorldAffairsभारत1 2 ปีที่แล้ว

      @@aprilsmith8924 Bhai antibiotic injection bhi lagane honge 5 days

    • @user-fn5gr1re4w
      @user-fn5gr1re4w 2 ปีที่แล้ว +2

      So true. I've been suffering for 7+years with monthly UTI'S 😫 and I didnt know I was and it was so painful and hard to urinate and bleeding. I have to take meds every month for my UTI'S. One day I had one so bad and I was starting to bleed and had to see a different doctor and they had marked my specimen as contaminated and I was like no that can not be because I am actually bleeding and the pain is horrible and I have them every month. They re did the urine culture and this time it wasn't marked contaminated so they gave me meds

  • @sunayanastha6907
    @sunayanastha6907 3 ปีที่แล้ว +3

    What is this streaking technique called?

    • @creativeman17
      @creativeman17 4 หลายเดือนก่อน

      Inoculation of agar plates to identify organisms (bacteria) present in the urine sample.

  • @lelavet6829
    @lelavet6829 3 ปีที่แล้ว

    Thank you so much
    I learn a lot from your videos 🙌

  • @theannyoonwee8550
    @theannyoonwee8550 ปีที่แล้ว

    Thanks you for sharing

  • @Gri-n9l
    @Gri-n9l 2 ปีที่แล้ว +3

    We work entirely different. How do you find " clean" colonies when you are striking like this? We do 3x striking with 3 Loops and we use the same loop for the first striking on Mc and blood with no problem. How many different bacteria do you test? When you have 3 ( prxx, ec and Enterococcus for example in same count) do you test or is it contamination? Nice video!!!

    • @basher94s
      @basher94s ปีที่แล้ว

      Yeah..but she could have perform sub culture from this one

  • @RanaRana-qg5kt
    @RanaRana-qg5kt 3 ปีที่แล้ว +2

    Why you putting in CO2 incubator

  • @ashankalad538
    @ashankalad538 2 ปีที่แล้ว +2

    How much temprature of incubator??

  • @MarysZenDoodleArt
    @MarysZenDoodleArt 3 ปีที่แล้ว

    Thank you 😊

  • @TheAcvivek
    @TheAcvivek 3 ปีที่แล้ว +2

    is the urine sample you used diluted or undergone any form of pre culture preparation? Thank you for explaining!

    • @MedicalLabLadyGill
      @MedicalLabLadyGill  3 ปีที่แล้ว +2

      Good question! Urine should not be diluted or treated in any way before culture.

  • @MusicmeetsTravel
    @MusicmeetsTravel 3 ปีที่แล้ว

    Explained very well

  • @rddtdx444444ex
    @rddtdx444444ex 2 ปีที่แล้ว

    What if you test negative for leukocytes and nitrites but bacteria was present and the urine culture was from 25-50000 Cfu/ml of E. coli. Im a male

    • @xsparik
      @xsparik ปีที่แล้ว

      Should co-relate with symptoms.

  • @saeednashad4660
    @saeednashad4660 3 ปีที่แล้ว

    wow very informative . love from pakistan

  • @MuruganSp-u1w
    @MuruganSp-u1w 6 หลายเดือนก่อน

    Gnr means

  • @sadikapatel2802
    @sadikapatel2802 2 ปีที่แล้ว

    Antimicrobial substances present in microbiology report meaning

  • @SHIVAMKUMAR-fx5gr
    @SHIVAMKUMAR-fx5gr 3 ปีที่แล้ว +1

    Can you make this video in hindi

  • @southpawl1
    @southpawl1 3 ปีที่แล้ว

    Great video

  • @elizabethwanjirundungu
    @elizabethwanjirundungu 2 หลายเดือนก่อน

    Gnr means what?

    • @MedicalLabLadyGill
      @MedicalLabLadyGill  2 หลายเดือนก่อน +1

      Gram negative rod also known as Gram negative bacillus

  • @chithravivek472
    @chithravivek472 3 ปีที่แล้ว +1

    Is there any vaccancy for microbiologist

    • @MedicalLabLadyGill
      @MedicalLabLadyGill  3 ปีที่แล้ว

      There are always positions available for medical laboratorians! You will never want for a job!

    • @saiyednaved
      @saiyednaved 3 ปีที่แล้ว

      @@MedicalLabLadyGill position available @your lab?

    • @MedicalLabLadyGill
      @MedicalLabLadyGill  3 ปีที่แล้ว

      @@saiyednaved not currently but all our neighboring hospital labs have positions available.

    • @saiyednaved
      @saiyednaved 3 ปีที่แล้ว

      @@MedicalLabLadyGill kindly provide adress or e mail adress so I can apply for the same.

  • @sharnivinson7961
    @sharnivinson7961 2 ปีที่แล้ว

    knowledge you share are a true blessing Doc Kham on TH-cam .

  • @isaacchulu8120
    @isaacchulu8120 2 ปีที่แล้ว

    Am so helped,, am looking for someone to help me with an Incubator

  • @KrishnaKUMARMaurya-ze1kk
    @KrishnaKUMARMaurya-ze1kk 2 ปีที่แล้ว +1

    What should i do

  • @mirachaib3633
    @mirachaib3633 3 ปีที่แล้ว

    Thanks ♡♡

  • @videoerxxx1230
    @videoerxxx1230 2 ปีที่แล้ว +1

    What does GNR mean?

  • @mirzasikandar1680
    @mirzasikandar1680 2 ปีที่แล้ว

    Mucoid colonies on top klebsiella 8:02

  • @AliKing-n1q6t
    @AliKing-n1q6t 11 หลายเดือนก่อน

    One question from my side

  • @KrishnaKUMARMaurya-ze1kk
    @KrishnaKUMARMaurya-ze1kk 2 ปีที่แล้ว +1

    I take plenty of water in a day approx 2. 0 litter

  • @dr.gaballaaziz8929
    @dr.gaballaaziz8929 2 ปีที่แล้ว

    2nd step in antibiotics

  • @ابوالقاسمعبدالرحمنعبدالله

    🥰🥰🥰🥰🥰🥰💟💗💗💗

  • @KrishnaKUMARMaurya-ze1kk
    @KrishnaKUMARMaurya-ze1kk 2 ปีที่แล้ว

    Reply me soon

  • @paarwathiraj9350
    @paarwathiraj9350 2 ปีที่แล้ว

    I think it is kleb

  • @irfanullah-uk3ks
    @irfanullah-uk3ks 2 ปีที่แล้ว

    Thnkeo thnkeo ma'am,for your efforts ,,,May God bless you ,and may you get success in evey field of life ,,

  • @MuruganSp-u1w
    @MuruganSp-u1w 6 หลายเดือนก่อน

    Gnr means

  • @MuruganSp-u1w
    @MuruganSp-u1w 6 หลายเดือนก่อน

    Gnr means

  • @MuruganSp-u1w
    @MuruganSp-u1w 6 หลายเดือนก่อน

    Gnr means

  • @MuruganSp-u1w
    @MuruganSp-u1w 6 หลายเดือนก่อน

    Gnr means

  • @MuruganSp-u1w
    @MuruganSp-u1w 6 หลายเดือนก่อน

    Gnr means

    • @creativeman17
      @creativeman17 4 หลายเดือนก่อน

      Gram negative rod

    • @MuruganSp-u1w
      @MuruganSp-u1w 4 หลายเดือนก่อน +1

      @@creativeman17 thank you