@@MicroChemsExperiments I wish I could have known your channel when I was in MTLA Program 😁 I come from Indonesia but been 2 years in Germany. How about you? I was wondering if you can also make a video about "Plaque reduction neutralization test" and more bacteriology test including the biochemical test. It would be really helpful for us who cant bring the notes to another country 😊
Rbar H, You are most welcome. We will prepare video for the enumeration of E. coli soon. You can enumerate E. coli by following steps: 1. Dilute sample to appropriate dilution. 2. Spread 0.1ml diluted sample on CCA plate. 3. Consider purple colonies as presumptive E. coli. 4. Confirm E. coli colonies by growing on EMB and sMac plates. 5. Count confirmed colonies as CFU. To get the video, please wait. We will work on it soon.
Could you tell what color colonies to check for salmonella, staphylococcus, pseudomonas with their respective agar medium. Also rest of the procedure would remain same ?
Hi, thank you for the video. May I ask for the peptone salt solution, can it be replaced with Buffered peptone water (BPW)? Also, I would like to ask is there a difference if I use 0.1% BPW, instead of BPW?
I have a doubt , for inoculation of E. coli on agar , we should use only one loopful of ecoli broth or for each quadrant we use individual loopful of ecoli
I have a one question from you... I collected sample from the sewage water,and followed with 3 times serial dilution with bglb broth and incubated for the identification of coliform bacteria.after the production of gas I isolate them into(0.1ml) macconkey agar and did indole test for them for the confirmation.in plates I've got 60 colonies,so the CFU /ml was calculated...6x10^5 CFU /ml ...so,I need to dilute them into three samples.like 10 CFU/ml,100 CFU/ml and 1000 CFU/ml...so can u give me an idea how to do that.... And my second question after diluted them,I need to those cultures to grow inside the nutrient broth for the DNA extraction,so,how can I inoculate them,I mean after getting dilution,we should do the culture again which I mention in 1st question like culture into the agar plates,if I take the one colony from the agar plate define 1 ml?I mean let say we culture the agar plate from the 100cfu/ml sample if I take one colony from them means that colony contain 100cfu?
Eelam Music Production, Its soo tough to explain here to solve your question. But I'm trying.... Situation-1: You got 60 CFU from 0.1 BGLB broth culture ****Ans to the first question: 1. To get 10CFU/ml sample, dilute 1ml BGLB culture (600CFU) into 59ml normal saline. 2. To get 100CFU/ml sample, dilute 1ml BGLB culture (600CFU) into 5ml normal saline. 3. To get 1000CFU/ml sample, you need to use lower dilution in the same way as done for 1 & 2. ****Ans to the second queation: A single colony contains millions of CFU. First dilute 1 colony in 100ml normal saline then culture 0.1ml from the diluted colony on nutrient agar. Count the CFU on nutrient agar plate. Now Calculate and dilute the normal saline as much as you need to find your desired bacterial concentration, on the basis of how much CFU you got on NA plate. If you dont get the expected result by following my guideline then try the bellow procedure: First culture a coliform bacteria in nitrient broth, centrifuge at 4000rpm for 5 min. Discard the upper broth layer, dilute the lower bacterial cell in normal saline, prepare McFarland 1.5 turbidity and match the turbidity of your bacterial cell using a spectrophotometer. Then dilute to desired concentration of bacterial cell as needed. Thank you.
Siti Salihah Mohd Saidi, No. Because CCA is highly selective for E. coli and Coliform and high temperature (121 degree) may destroy the selective ingredients of the medium. That's why, no need to autoclave the medium during the media preparation. Just boil to dissolve the content and pour into petri dish.
My Qus. pri-enrichment medium name ?? and Enrichment medium name?? which pri Enrichment and Enrichment medium use for detection of E coli o 157 H:7 test .??
@@MicroChemsExperiments how it is possible I am microbiologist I have done testing in certain sample where I have found colliform but the same sample when tested outside they didn't detect any coliform...can you plz sort out my quiery
Only perform Oxidase Test along with gram staining if you have facilities. Other biochemical test is not mandatory according to ISO reference method which is mentioned in this video.
Bokul Hossain, we combined two ISO methods with few modifications for E.coli test to simplify and strongly confirm the E.coli cell. You can't match full method to any of the refetence methods.
Firstly, analyze you sample according to this video. If you get the E.coli, streak several bacterial colony on "HiCrome EC O157:H7 Selective Agar Base" selective agar plate. Finally if you find dark purple to magenta coloured colony then you can interpret your test result of e coli O157 H:7 as detected.
I am a research student so can you please share the articles because I follow this video for the detection of E.coli. Please also explain what kind of the feed meal you use as a sample.
We have followed ISO 9308 & ISO 16649 to make this video. Please collect the handbook from ISO as we do not provide any pdf. In this video, we have tested a poultry feed meal sample.
@@MicroChemsExperiments you have any video or PPT on identification of E.coli by biochemical testing . Because I am University student And I need this PPT for presentation
This has helped me a lot throughout my training.
Thank you so much ❤
Keep uploading more stuff.
Glad it was helpful!
Very good ..well paced..accurate...professionally done..thx.
Thank you
You make a very good explanation and love the details! I hope you can keep up this good channel!
Thank you Ma'am. Keep supporting me. From which country you are??
@@MicroChemsExperiments I wish I could have known your channel when I was in MTLA Program 😁 I come from Indonesia but been 2 years in Germany. How about you? I was wondering if you can also make a video about "Plaque reduction neutralization test" and more bacteriology test including the biochemical test. It would be really helpful for us who cant bring the notes to another country 😊
@@hanazhafirahhanifah8175 Please knock on our Facebook page. Link is given in the description section.
@@MicroChemsExperiments Will sure check it later at night! Thank you in advance!
@@hanazhafirahhanifah8175 You are welcome. Stay with us
Thank you for this Explanation will help a lot on tomorrow's moke test and also for life time😌👌🏻
Thanks you for this information. I'm happy 😊 with you because I need this lesson
Thank you
Is this a Most Probable Number (MPN) method or Membrane Filtration Method (MFM)? Can it work for turbid water?
This has helped me a lots during my PT
Glad to know that
Please, what sample was used?
Thanks for the vdo..the audio and vdo quality are really good
It's our pleasure
Thanks for all your videos
Your videos are always helpful
Thanks 🙏🏻🙏🏻
LyRical WoRLD Sachin Yadav, you are most welcome. Thanks for being with us.
good job..... Thank you from Libya
Thanks for your support
if we have a swab sample, how to prepare a sample?
The way of expression was SMART in details we will wait you....
Thank you so much for your support.
sorry can you give the specifications of EMB AGAR MEDIA you used?
The video quality & background sound was awesome 💖
@Meftahul Jannat,
Happy to hear that! Thank you so much. Be with us.
after we strake , it fills the petri dish, not in scratches, is that contamination? Or too much bacteria?
In this, you had prepared peptone salt solution, did you not use it in this entire procedure? Can you tell why was it prepared in this procedure?🤔
Watch carefully..it is used as diluent for the sample
Very informative content. Thank you ❤
Stay with us
Thank you for the video. Is there a method for E coli enumeration?
Rbar H, You are most welcome. We will prepare video for the
enumeration of E. coli soon. You can enumerate E. coli by following steps:
1. Dilute sample to appropriate dilution.
2. Spread 0.1ml diluted sample on CCA plate.
3. Consider purple colonies as presumptive E. coli.
4. Confirm E. coli colonies by growing on EMB and sMac plates.
5. Count confirmed colonies as CFU.
To get the video, please wait. We will work on it soon.
Rbar H, please find the E. coli enumeration video. Video Link: th-cam.com/video/aBEoNeboAHs/w-d-xo.html
Could you tell what color colonies to check for salmonella, staphylococcus, pseudomonas with their respective agar medium. Also rest of the procedure would remain same ?
We will make video soon
Nice better way of teaching 👍
It's our pleasure. Thank you so much.
Oh yeah perfect thanks for this.
Thanks
Which colour will show negative results of e coli
Superb art of work. Keep it up
Thank you very much. Stay with us.
Considering EMB and sMAC is used after 48 hours, is there need to prepare it on first day?
Not necessarily
Hello dear. I have a question in cca agar plate how we can differentiate ecoli and coliform because cca is suitable for both e coli and coliform
E.coli will appear as violet blue colonies whereas coliform shows pink colonies
Hi, thank you for the video.
May I ask for the peptone salt solution, can it be replaced with Buffered peptone water (BPW)? Also, I would like to ask is there a difference if I use 0.1% BPW, instead of BPW?
You can use BPW as diluent. No problem with it
@@MicroChemsExperiments thank you
Can we use these procedures for water testing too???
Yes
This is too much noise for me, background music
Ok we will make it lower in the future
Same remark
Hlo mem your explanation is very well
Thank you. Stay with us
In the procedure how to calculate the e coli?
I have a doubt , for inoculation of E. coli on agar , we should use only one loopful of ecoli broth or for each quadrant we use individual loopful of ecoli
You should use one loop full of E. Coli and draw the first quadrant. That's enough. No need to take again for other quadrant
I have a one question from you...
I collected sample from the sewage water,and followed with 3 times serial dilution with bglb broth and incubated for the identification of coliform bacteria.after the production of gas I isolate them into(0.1ml) macconkey agar and did indole test for them for the confirmation.in plates I've got 60 colonies,so the CFU /ml was calculated...6x10^5 CFU /ml ...so,I need to dilute them into three samples.like 10 CFU/ml,100 CFU/ml and 1000 CFU/ml...so can u give me an idea how to do that....
And my second question after diluted them,I need to those cultures to grow inside the nutrient broth for the DNA extraction,so,how can I inoculate them,I mean after getting dilution,we should do the culture again which I mention in 1st question like culture into the agar plates,if I take the one colony from the agar plate define 1 ml?I mean let say we culture the agar plate from the 100cfu/ml sample if I take one colony from them means that colony contain 100cfu?
Eelam Music Production, Its soo tough to explain here to solve your question. But I'm trying....
Situation-1: You got 60 CFU from 0.1 BGLB broth culture
****Ans to the first question:
1. To get 10CFU/ml sample, dilute 1ml BGLB culture (600CFU) into 59ml normal saline.
2. To get 100CFU/ml sample, dilute 1ml BGLB culture (600CFU) into 5ml normal saline.
3. To get 1000CFU/ml sample, you need to use lower dilution in the same way as done for 1 & 2.
****Ans to the second queation:
A single colony contains millions of CFU. First dilute 1 colony in 100ml normal saline then culture 0.1ml from the diluted colony on nutrient agar. Count the CFU on nutrient agar plate.
Now Calculate and dilute the normal saline as much as you need to find your desired bacterial concentration, on the basis of how much CFU you got on NA plate.
If you dont get the expected result by following my guideline then try the bellow procedure:
First culture a coliform bacteria in nitrient broth, centrifuge at 4000rpm for 5 min. Discard the upper broth layer, dilute the lower bacterial cell in normal saline, prepare McFarland 1.5 turbidity and match the turbidity of your bacterial cell using a spectrophotometer. Then dilute to desired concentration of bacterial cell as needed.
Thank you.
if sorbitol agar we dont have so we use mackonkey agar ?
Yes. Use MacConkey Agar
Awesome.. very helpful. 🙏
Thank you.
I understand all things Thank you 👍👍👍👍👍👍👍👍👍👍👍🔥🔥🔥
You are welcome!
For CCA, it don't need to be autoclaved before it is pour into the petri dish?
Siti Salihah Mohd Saidi, No. Because CCA is highly selective for E. coli and Coliform and high temperature (121 degree) may destroy the selective ingredients of the medium. That's why, no need to autoclave the medium during the media preparation. Just boil to dissolve the content and pour into petri dish.
@@MicroChemsExperiments aaaa I see, thank you for the explanation
@@sitisalihahmohdsaidi2583 You are most welcome. Thanks for being with us.
Vry vry use full tq so much ma'am
Thank you
@@MicroChemsExperiments mam sds page podunga plzz Tamil la sollunga mam plzz
@@SriRam-cw8px Please write in English
@@MicroChemsExperiments mam English avlova varathu Tamil sonna purenjipa so atha keta Tamil la podunganu
E coli O157 Enrichment Medium
Can buffered Peptone Wtaer with Pyruvate (bPWp) and ACV (Acriflavin-Cefsoludin-Vancomycin) be used ?????
Just use Selective media for E coli O157.
My Qus.
pri-enrichment medium name ??
and Enrichment medium name??
which pri Enrichment and Enrichment medium use for detection of E coli o 157 H:7 test .??
@@ajitpatil2153 Enrichment Broth: mTSB with Novobiocin.
Pre-enrichment is not necessary
Thanks
@@ajitpatil2153You are welcome
Great job 👌
Thanks
Very well explain 👌
Thank you
Can we use this method for E.coli detection from hand?
You can use
Can I ask one question is this possible that your place is not cleab but still your coliform count is absent .
Yes. This is possible.
@@MicroChemsExperiments how it is possible I am microbiologist I have done testing in certain sample where I have found colliform but the same sample when tested outside they didn't detect any coliform...can you plz sort out my quiery
Thanks for sharing!!!
My pleasure!!
Can we use this method for E.coli detection from water sample..
Yes. You can
What about double strength mac conky agar
We will make video later
Hello. I have a question. If we use this microbiological test for E.coli detection, do we also need to perform biochemical test?
Only perform Oxidase Test along with gram staining if you have facilities. Other biochemical test is not mandatory according to ISO reference method which is mentioned in this video.
@@MicroChemsExperiments Noted. Thank you so much !
Indole test can be performed?
Good explanation
Thank you
Thank you a lot!
You're welcome!
thanks
can i get pdf for this test,?
Bokul Hossain, we combined two ISO methods with few modifications for E.coli test to simplify and strongly confirm the E.coli cell. You can't match full method to any of the refetence methods.
Dear sir I want all microbiological tests videos for drinking water
Okay. Stay with us
How to detect second strain e coli O157 H:7 ?????
plzz reply
Firstly, analyze you sample according to this video. If you get the E.coli, streak several bacterial colony on "HiCrome EC O157:H7 Selective Agar Base" selective agar plate. Finally if you find dark purple to magenta coloured colony then you can interpret your test result of e coli O157 H:7 as detected.
@@MicroChemsExperiments e coli o157 h:7 selective media name plzz ??
@@ajitpatil2153 Already mentioned in the first reply. The name is: HiCrome EC O157:H7 Selective Agar Base
Did u do this practical for o157 Ecoli
I am a research student so can you please share the articles because I follow this video for the detection of E.coli. Please also explain what kind of the feed meal you use as a sample.
We have followed ISO 9308 & ISO 16649 to make this video. Please collect the handbook from ISO as we do not provide any pdf.
In this video, we have tested a poultry feed meal sample.
@@MicroChemsExperiments Thank you so much.
@@entertainmentpoint4242 It's pleasure
Wich sample you give for the this test...plz tell me
Prajwal Nimbulkar, we tested a feed meal sample in this video
@@MicroChemsExperiments thanks for replying
Thankyou so much
@@prajwalnimbulkar267 Its pleasure. Stay with us.
please what is the full meaning of CCA
Chromocult Coliform Agar
Wow nice
Thanks
Name of this type of method ????
The reference of the method: ISO 9308-1
Is this biochemical test ?
No sir. This is Microbiological test
@@MicroChemsExperiments you have any video or PPT on identification of E.coli by biochemical testing .
Because I am University student
And I need this PPT for presentation
@@hassaan7990 No PPT is available. Search on Google
@@MicroChemsExperiments actually I searched it . But I could not find it. Can you please help me 🥺 for collecting the data .
@@hassaan7990 No sir. we can't help at this moment. Sorry
How much tissue need for the purpose of study?😢
Thank you
Most welcome
Very nice
Thank you
Can we do internship here
No sir.
If you have maual for thia i want
Sorry. We don't provide any document
Where are u from mam ??
We should not discuss it. Please ask questions related to the video
How to prepare the result and report to the given sample
Aanchal Gahalot, the result is E. coli detected.
The the result should be reported as 'Detected/25g'
Please give me the following information
detection of E- coli o157 H:7
Pri- enrichment medium name -??
Enrichment medium name -??
Already mentioned
Please give calculation part
❤️
Thanks
Please make video without music.
Okay
Biochemical tests
No need as per ISO SOP.
Hi
Hello
thankful for all video it is helpful am very interesting for more help
Thank you
Salmonella