Spread Plate Technique for Colony Counting_A Complete Procedure (Microbiology)
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- เผยแพร่เมื่อ 22 ส.ค. 2020
- Spread Plate Technique is the most common and widely used method for colony count experiments, both for bacteria and fungi. This video presents a detail and complete procedure for colony counting of bacteria and fungi by Spread Plate Technique.
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Thank you sir, explained in easy manner😍
You are welcome. Stay with us
Very helpful for beginners...thank u
Thank you
Thank you so much 💖💖
This video is very helpful
Thank you
your videos are very effective for beginners....we want more video about Food chemistry and microbiology
Raju Ahmmed, thank you for your comment. Still long way to go. Please mention which tests do you want to see in future.
thank you!!!
You are welcome
Your channel is amazing. Bcoz of your explanation I improved myself. Thank you Show me more experiment of chemistry of water test.
Thank you for your comment. Please leave a comment with water test parameters.
Really helpful vdo it was
Thank you
Wonder 😊
I am nursing students sir..so this video is very important and very useful thank you so much for explain
Thank you. Stay with us
Amezing
Thanks
çok kral adamsın 😃
Thank you 💯💯❤️
🙏🏻
You are welcome
nice video btw👍 may i know what sample do you test? is it not diluted first?
We tested water sample which was not diluted.
So nice the explaining way , Thank you so much, love from Pakistan
Thanks
Amazing channel
Thanks
@@MicroChemsExperiments Which app is best for video editing please recommend
@@MicroChemsExperiments Please give me Email id
@@medicineknowledge6710 filmora
@@medicineknowledge6710 mail.mic.chem@gmail.com
by sterilizing the glass rod outside the cabin and then moving it inside, wouldn't that contaminate it?
Thanks you for video,this chanel is very good.
Can you help me and explane me on by video how can I make stock culture and possible to storage and control. Thanks you very much
th-cam.com/video/RNvtAjeNqTM/w-d-xo.html
for sample weighing, should it be done in the laf?
No need to use laf for sample weighting if you use a clean and controlled room.
I have a one question from you...
I collected sample from the sewage water,and followed with 3 times serial dilution with bglb broth and incubated for the identification of coliform bacteria.after the production of gas I isolate them into(0.1ml) macconkey agar and did indole test for them for the confirmation.in plates I've got 60 colonies,so the CFU /ml was calculated...6x10^5 CFU /ml ...so,I need to dilute them into three samples.like 10 CFU/ml,100 CFU/ml and 1000 CFU/ml...so can u give me an idea how to do that....
And my second question after diluted them,I need to those cultures to grow inside the nutrient broth for the DNA extraction,so,how can I inoculate them,I mean after getting dilution,we should do the culture again which I mention in 1st question like culture into the agar plates,if I take the one colony from the agar plate define 1 ml?I mean let say we culture the agar plate from the 100cfu/ml sample if I take one colony from them means that colony contain 100cfu?
Eelam Music Production, Its soo tough to explain here to solve your question. But I'm trying....
Situation-1: You got 60 CFU from 0.1 BGLB broth culture
****Ans to the first question:
1. To get 10CFU/ml sample, dilute 1ml BGLB culture (600CFU) into 59ml normal saline.
2. To get 100CFU/ml sample, dilute 1ml BGLB culture (600CFU) into 5ml normal saline.
3. To get 1000CFU/ml sample, you need to use lower dilution in the same way as done for 1 & 2.
****Ans to the second queation:
A single colony contains millions of CFU. First dilute 1 colony in 100ml normal saline then culture 0.1ml from the diluted colony on nutrient agar. Count the CFU on nutrient agar plate.
Now Calculate and dilute the normal saline as much as you need to find your desired bacterial concentration, on the basis of how much CFU you got on NA plate.
If you dont get the expected result by following my guideline then try the bellow procedure:
First culture a coliform bacteria in nitrient broth, centrifuge at 4000rpm for 5 min. Discard the upper broth layer, dilute the lower bacterial cell in normal saline, prepare McFarland 1.5 turbidity and match the turbidity of your bacterial cell using a spectrophotometer. Then dilute to desired concentration of bacterial cell as needed.
Thank you
👍👍
Thanks
Hi, this video is good. Would you please add content about TPC (total plate count). please give some protip too. also would you mind explained how to fix the error like
1) amount of colony on agar isn't linear with the dillution. I mean, if we count the cell in the highest dillution, the amount of colony isnt the lowest.
2) contamination on agar plate
the last, would you please tell us about how to storage agar plate and prevent it from wet surface agar condition. thanks in advance :)
1. Vortex, each time, just before taking the diluted sample for inoculation
2. Aseptic techniques should be followed to avoid contamination. Please watch this video: th-cam.com/video/YI2jXT0Kld8/w-d-xo.html
3. Place the prepared agar plates inside the refrigerator keeping upside down to avoid wetting agar surface
**Already working with the video for TPC. Stay with us.
For avoiding contamination, the best way is to use selective media. As for instance, LB or Mackonkey for E.coli.
@@raktimchowdhury8317 I've been using selective media, but contamination still happens. turns out, our lab contaminated with bacillus spores. how to clean those source contaminant?
I just did this today
Great.
What will be the normal range??
Is it okay to keep the cover petri plate at the surface the LAF?
Yes. No problem with it if you keep the lid in a manner that the inner side contained facing upper side.
Hi, Ihave question, Petri dishes with agar ready to use (ALOA) are dried before inoculation at 37oC / 1h. Is it then immediately taken out of the incubator, or is the incubator set to room temperature after drying to avoid evaporation?
Blue S, thanks for your question. You can take out dried agar plates from the incubator immediately and adjust the temperature of the agar plate at 25 degree Celsius before inoculation.
@@MicroChemsExperiments and after closing the plates immediately, there is water vapor on the lid, doesn't it bother you?
@@lilianas9702 of course it is irritating. We normally jerk the lid on the Biosafety Cabinet surface to drop the water droplets and keep the lid open for few minutes in the Biosafety Cabinet.
@@MicroChemsExperiments Thank you very much for the answers
@@lilianas9702 you are most welcome. Thanks for being with us.
what are the quantitative techniques of growth promotion tests for solid media
Both of pour plate and spread plate technique
So serial dilution is not needed for the spread plate method ?
Same question
Yes, serial dilution is needed if you know that your sample is concentrated. however if the sample is already dilute, then not required for spread plate method.
how can we prepare sample 1
Thanks for this video. However the background music is a bit too loud and distracting from the main focus. As someone with ADHD this affects my learning. This is constructive criticism. Once again, thanks for this video.
Thank you so much. In future we will make the background music lower.
I've one question. After spreading, bacteria isn't appearing as colonies, it is appearing as white color overgrowth all over the agar plate surface. Can you tell me the reason and what should I do?
Some bacteria form mat on whole agar surface. Spread your sample on triplicate (3 plates) to get at least one plate with isolated colonies
what is sample 1 can anyone explain plz
Plzz Ignore the same and focus on the method.
Hello sir
I need a help regarding isolation of my acetobacter species. From grape source I got suspected colonies, but after several sub culture I couldn't get a pure isolate,what can I do in this case.
Looking forward for your reply
Thank you
Streaking method is the best method for getting isolated pure colony. So try to get the pure culture by following our video: th-cam.com/video/bm99zrq3ijo/w-d-xo.html
Then send the picture of the culture plate in our facebook page.
3.21 cfu/g is my reading
.: Then how many colonies are there
There are about 3 colonies in 1g of your sample.
WHY ARE WE INVERTING THE AGAR PLATES BEFORE PUTTING IN INCUBATOR
To avoid drying the semi-solid agar media
@@MicroChemsExperiments ty
@@mohitswami2116 wc
Bcoz prevent condensation