Coronavirus Test: Real time RT-PCR - Animation video
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- เผยแพร่เมื่อ 20 ก.ย. 2024
- I make animations in biology with PowerPoint, this animation video is about the standard coronavirus test, real time RT-PCR method, which is a laboratory technique combining reverse transcription of RNA into complementary DNA, and amplification of specific DNA targets using polymerase chain reaction (PCR).
Coronavirus Antibody Test - Animation video :
• Coronavirus Antibody T...
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Certain terms such as genome, lysis, and polymerase are mispronounced, because the voiceover is a synthesized audio.
Very nice.. please explain Antigen Test of Corona
What do E and N gene code for in coronavirus?
@@shraddhaeknathkhaire9787 you mean antibody test? Its so easy if you have antibodies in your blood (serum) it will travel through the cellulose membrane of the test kit which there are stationary anti immunoglobulins made from goat blood that will attach to your antibodies (if you have them) which will result in a colored line in the test kit. So how colored line formed is like this: antibodies to corona+anti-immunglobulins with colloidal gold->travels through test membrane->anti-Ig in test region attaches to that complex=colored line. Its called sandwich immunochromatography.
Very nice info, i have confusion on RNA extraction, thnx
Thought that might be why!
As a Doctor , Im saying you have explained something that even my Professor cannot do ... Hats off buddy
Hello prakash sir where go u song ur practice I am a mbbs student
Greetings for that Professor! 😅
How are you doi a true test
In other words, the professor AND the doctor are both clueless about what's really on the tip.
that's crazy. i didn't understand a thing. i truly admire those smart people that are able to come up with something so complicated. you guys are the coolest.
Im in 10+2 studying for medical and they taught us about this basic idea test of pcr test. They taught us only three steps 1.denaturation. 2.annealing. 3 extension
@@carrybhai7312 yes.
Yessa❤️
Not every people working on these things is super duper smart. Running the test doesn't necessarily mean you understand what you're doing. It can be done mechanically by anyone who can memorize the steps and have basic knowledge of it. Science is a collective effort of many researchers throughout our history. That's why on one side we have exceptional scientists who are more famous for their amazing contributions and on the other side we have regular scientists who aren't as geniuses but they also contribute to the human knowledge working with peers as teams.
I understand completely as I studied this science for a long time
I’m a biologist and currently taking a master‘s degree in Biotechology and this video is very well done and accurate!
I just finished mine.
@@uzzifennelli598 congrats!
Funny reading replies to commenters who have disappeared.
i'm currently doing my masters and these videos are great if you need visual aid for learning. i haven't found too many good bt animation channels on yt yet but i'm on it...
How much time does it take to complete the whole process?
As a biologist,I have to say this is a fantastic animation for RT PCR! Thanks for posting!
@@sivabalankesavan4339 Interesting, I'm going to look into this.
YES!!
this is like a whole semester lecture and lab condensed in a youtube video.
@@devinify The power of good and accurate animations in modern teaching!
As a biology student i felt same as you!!
As a freshman science major I literally started freaking out when watching this because we had a lab in biology where we did this with fish dna. Its so cool to see stuff that I did in class being done in real life.
my eyes just glazed over after 10 seconds. it is like a whole other language. We need more people like you and for the future of medicine..good luck.
Schnurbs while you become an old man science person, it is really cool, believe me..
ME TOO!
I didnt do anything that cool in school, although I was a a community college so...
Goood!!!
Me: curious how they test covid something.
After watching the video.
Me: staying at home is simple.
Lol I agree! Though, working with these type of techniques is not necessarily difficult. It´s more time consuming and relatively expensive.
shree umate unfortunately, not everyone has the privilege to stay home because of work and some countries like here in Sweden, there is no quarantining
Best Choice . I too will follow that .👌
Offcourse same feeling
Exactly, but in this moment, some stupid people think that the virus is not real and it is a government plan for our manipulation... :/ Greetings from Mexico!
After watching this my sympathy for biology student is dramatically increased!
Me a bio student
The worst part is that this isn’t nearly as detailed as the exams lol
We are biology engineers
Yeah I feel bad for the labs right now...🥺 so much workload !
Thanks
I m a happy engineer now.... Respect 🙏
after 1 minute, i was just pretending to understand everything.
I just removed my headphones imma just watch the pictures
Same here.
You just pointed me out.
I just thinking business is best instead of science 😆
How long did you need?! For me 10 seconds was enough :))))))
Whoh! I never thought class 12 biology chapter 6 would have soo much importance in real life 😆😆. I legit understood everything.
It is not class 12 biology. It is not so simple
Ya true👍👍
@@rahulchougule9277 it is class 12 biology. The whole thing is there in ncert
This feels like one of those exam questions where the solution is sequential, 1 wrong step and the whole thing is bust.
Like Calculus equations.
Lol now I understand the importance of those molecular biology lectures in med school !
I like how you're trying be "cool" by using the word medschool....
He might really be a doc
As bioinformatics student I was really impressed by this video.Take bow for your efforts sir 👌👌👌👌
Which college?
Even if you have to animation app, the time and effort for organising the material and learning about it is immense. This is gold for learners. Thank you
For 1st 4 -5 min I was able to understand and regain my knowledge which I've studied in my 12th grade and later that everything just bounced over and messed up .. I truely appreciate your work your job 🙏🏼🙏🏼.
How many people are understanding nothing? The one who is understanding everything is really great.
As a biology PhD student, I actually didn’t not understand the inner structure of the filter membrane until watched this video! Well explained!
Thanks to all the Scientists and Engineers who stayed in school and contributed to the development of such a cool technology.
No kidding when the doctors say the test takes time!
It actually does not take that much time. Depending on what instrument you use you could get a pcr result in under 2 hours. Technology and Automation has come a long way over the last decade in PCR.
40 minutes nowadays, Doctors don’t do the test. Lab personnel does.
It’s really cool to see that what we did in bio lab last semester is being done in real world scenarios
I m a microbiology student nd yesterday i read theory of pcr technique in my biochemistry book nd today youtube recommended this now i fully understood the topic. thnku so much it helped me understood practically for my exams🙏
I love biology to! Be a great microbiologist. 👍
*Suddenly everyone in the comments has a degree in this field*
I do not agree.
ya man
Maybe some. Not everyone
Including a few in civil and mechanical engineering.....🙂
I learned this in bio 189 my freshman year of college
I m in class 12th and have a chapter biotechnology principles and processes in which PCR is explained and I m really shocked how little we have learnt from our ncert bio book about this topic...😨😨
Same here
Yess!!
I am also in 12 but i have Optional Subject Biotechnology and I read it.
Yeah.....it was just given PCR...and in three steps....it is done....but see here....it was like how little we know 🙄🙄......
But one more thing.... during watching of this video....it feels like our teacher is explaining molecular basis of inheritance Chapter 😂😂😂😂😂
We cannot understand until we do undergraduate courses in bio. In 11th 12th is basically an introduction to collage courses. So wait
I studied Civil engineering and this video information is correct and accurate!
This is the most accurate reply ive read so far😂
Yes . I am a mechanical engineer and I too certify that it's correct . Anyway I continue to stay at home .....!!!
I am a practicing Chartered Account and I certify this video to be correct and accurate!
Civil engineers learn this stuff???
Lol good one mate 😂👍
Tomorrow I have a job interview, and this video helped me remember many aspects of PCR that I had forgotten, I am very grateful!
how was the interview
What job?
All the best bud
Did you get the job?
Each and every word he spoke passed over my head.. 🤨🧐😶
So I'm sure u must not from science background.
The first video I found after a long search that actually explains how the detection is happening. Thank you!
This would be the last time I looked at a video that explains Microbiology or Virology. I watched 6 mins, I came here for explanation of how the virus is Diagnosed, in the 6 mins I needed 100 further explanations.
I'm a business guy who has to create slides explaining this stuff to investors, and this good work is invaluable.
As a biologist and epidemic expert, I must say that the animation is very simplified and wonderful.
I swear i didn't understand anything but enjoyed the video 😭🤣
Found this to be a complex procedure, hats off to the number of samples being taken on a daily basis
Recently switched my field from chemistry to biochemistry and ran couple of PCR reactions for protein expression. I liked this video very much. Hats off!
How do they seperate corona virus rna strands from non-corona virus rna ?
Through the sequence of the Primers. The primers are like 20-30 basepairs long, and only bind to their complementary sequence
We studied this in our final year of Bachelor's degree and were about to give the final exams before we went into complete lockdown. This is SO exciting to watch. I cannot get over how simple yet elegant these techniques are. Thank you so much for this!
This makes me feel like when your're in class and don't understand a word the teacher is saying, but then you pretend to get it and then hope that when you get home and read upon the topic that you'll get it then and won't feel like a total dumb a..s.. LOL
ye
I'm a Medical Technology student, and I can say that this video is simple but accurate. Also, great illustrations! It really allows me to fully grasp my learnings
Literally the best and easy to understand video explanation of RT-PCR I have ever seen. Thanks a lot for making this!
I have a Master's Degree in Management and I have to say this is absolutely accurate to point. With very little detail dispersion.
As a dumbest student, I just felt like I was in my school lecture of chemistry.
It's Bio technology bro
I just felt the need to go to CR after watching this PCR.
I think this is one of the best video , nicely explained in a simplified way of the entire procedure and process of PCR !!! Thumbs up to the creator
Brilliant step by step guidance and explanation. I love it!
Wow such a great explanation. As I am a biotech student feels like can do it in lab with proper precaution
same, I do biochem
Precisely!
A biotech student??
@@abdulsoabirshaani3629 students who are majoring in Biotechnology
This perfectly deviates us from asking about false positives
This is how they "soft" the test, by making it pretty, its a amplification and copy machine, just that
m a junior microbiologist and'll complete my graduation this year this video is really very appreciating dude👌
vaishnavi bhardwaj
Good job
I'm a medical laboratory technician and I have to say this video is really well done the detail is spot on.
As a doctor I would say this is an amazing video to understand Covid-19 Test
Its not Only for Covid 19 Testing.
Best video I have found so far! Thank you!
As a Biochemistry student,we have learnt this as part of lab practical.. Bio subject is very interesting
As a pathology officer it is very easy way to explain all about the PCR step wise . The animation accurately shows all steps involved and explained in very simple way .
The RT-PCR is simple method , many non technical people may find difficulties but we have been doing this for other molecular tests before.
This was really helpful thank you. We were given an assignment to find out everything we can about covid 19. From the pcr test to the how the vaccine is made and how it works. This was helpful
Thanks for this supreme animation and explantion!!! 25 amplification rounds: 16.777.216 copies of target DNA. 40 amplifications: 549.755.813.900 copies. To much cycles (for e.g. 40) remains in way too much false positive results. This is also important, to know. A corona-positive result is only reliable, if there where less cycles, for e.g. up to 25.
Why would you amplify to 60 cycles which gives a 100% positivity? What could justify 20 more cycles? Is a positive is just how much you can observe?
Please help me understand.
holy moly. I just understood the PCR concept which i struggled so much in biology and i never thought i could understand >_
Amazing video! Fantastic explanation and fantastic animations. Really well done!
Imagine doing this for a couple thousand people each day.
Pretty simple when you're doing it. Just takes a long time to process. Can be done on a 96 or 384 well plate. In my experience it's about 4 hrs per plate plus about an hour per 92 samples to prep them and get them into the plate
exactly.....I doubt sincerely they do this for every single test. NO WAY.
@@tiberio1352 they have pipetting robots, who do the entirety of the pipetting fully automatic. There is no single person doing all these tests, that would take way too long
@@felicia6438 do you put their names on the testing tube ?
I just found this channel on TH-cam. And I am really impressed with the video. This is one of the best PCR videos I've ever watched. The explanation is concise and simple. The voice is quite clear, so as an Indonesian, which English isn't the mother's language, I don't have any difficulties to listen and understand your explanation. Thank you for your effort to make this video and share to us. Sorry for the bad grammar ;)
I love how biotechnology works.
Woah! i don't think i'm ever going to complain about the report of the test being late!
this guy is a genius
I thank to biologists/technicians doing millions of this cumbersome test everyday. These tests are hope for mankind. Thank you also for this complex informative animation and explanation.
This is really nice explanation of RT-PCR. Well done for this. Only thing I would have liked is if you would explained thresholds of 24 and 35 CT that are important in Covid-19 testing.
Why were they using CT of 40 and above?
Can you do an animation for the TMA method of detecting ? Please
2:12 for an unbalanced centrifuge
I was thinking the same thing. Don’t put that tube in there alone!
hahaha literally paused the video to see if I could find anybody who spotted that as well
oh i'm sure they'll have enough samples now, what will all those cases...
This gain huge respect to people who research and test such complicated stuff.
Never expected that complexity 😂
Right, but this process is happening very fast the only problem is the PCR amplification that takes more time than other processes
Such a long process, hats off to these people doing all these tests.
Oh my... I got lost at 1:05
😂😂😂
Yes, oK ok ok, i'll stay home.
Finally, a video that explains this process in layman's terms!
Beautiful Animation with great explanation. Thank you.
No wonder its expensive and time consuming to do a PCR test. Hats off to all the lab technicians around the world doing thousands of this procedure a day!
Very articulate description. Excellent video.
I'm a microbiologist and this is one of the best visual representations of RT-PCR that I have come across. Really helpful for teachers. Thank you!
How long is covid detectable for when using a pcr test?
Very good explanation! With easy animations, it is understandable.
you make rt-PCR explanation really simple XD. if only my professor explains like this, I probably will ace my test in MCB haha
⭐Amazing Video ✨✨
Great explanation...
Thank you...!
holy cow. this is whole lot more complex than i thought it would be.
YOU are the one I am looking forward for years :) Love you literally!
As a molecular tech, this video does a fabulous job of explaining how the PCR machine works, as well as how wash, elution, etc buffers work! This is awesome! :))
My mind literally got blown by this video 🤯
This was excellent. Concise, beautifully animated and well explained. Kudos!
It's so nice and informative. Thank you for the detailed information in this video..
So this is the reason why I'm currently locked away in an isolation ward without any of the bare necessities, shivering in the cold with just a hard bed and a thin blanket.
Very well explained 🙌.. being a microbiologist I could relate all my theory related to RT-PCR 👍
very nice. I have been searching for a good video on the confirmation test for COVID19. This is the one.
Great Video ! Thank you for creating, I will share it on 🙏
As a layman, I am incapable to decipher most of the stuffs explained but I trust that this is a video well made as I can see those subject related viewers praising how good this video is.
A very comprehensive and lucid explanation of RT PCR. Great animations,great job!!
I am a master student in Biochemistry and work in a clinical chemistry institute. We do about 2000 tests a day and with this exact methode.
A super informative video that will make even average minds conversant. Kindly do more videos on different issues.
This is amazing. The animations explain qRT-PCR in a short and coherent manner. Thank you so much.
Now i come to know that why so many peoples found positive. 😳😳
Why?
The test is unreliabel. Many are false positiv!
test is very reliable and very sensitive.
Nice..... but bit lengthy......felt that it should have summarised well in points or steps at the end of video to make it understandable even for common audience, not only for the Biologists, Doctors and Lab techies.🖐
An excellent video. Within a very short time it explains everything .
One of the best RT-PCR videos so far on the Internet!
The danger is not what's in front of you but the real danger is what you're being distracted from
I learned this things for many many years and still didn't understand. Til i found this masterpiece. This is what should smart people do, explain a complex things with simple explanation.
I was searching for PCR related videos and found this in recommendation, nice keep it up
This video was amazing so accurately they explain rtPCR method...👍