ID Laboratory Videos: Antibiotic susceptibility testing

"Antibiotic susceptibility testing", 2015.
Produced by Tufts University School of Medicine Center for Science Education (CSE) as part of the Infectious Disease high school curriculum:
sites.tufts.edu/ctse/great-di....
The Great Diseases curriculum provides a framework for students to learn high school biology in a more approachable and life-relevant context, designed to improve science engagement and health literacy.
Supported by the National Institute of Allergy and Infectious Diseases (1R25AI098781-01A1).
Written by EmilyKate McDonough and Kayle Sawyer.
Filmed and edited by EmilyKate McDonough.
Actors: EmilyKate McDonough, Revati Masilamani, Berri Jacque, and Eleanor Fleming
Lab space and materials: Andrew Camilli, Katya Heldwein, Tufts CSE
Music: "Discomutante" - A Smile for Timbuctu (alterations made) and "Air Hockey Saloon" - Chris Zabriskie.
Animation consultant: Andy Musser

[@TimidAction]

for any poor soul who needs to find the 9 mistakes
Labeled the top of the agar plate rather than the bottom.
Did not keep the cotton tip of the swab sterile.
Did not orient the swab correctly when selecting a single bacteria colony from the stock culture plate.
Did not spread the bacteria colony evenly over the agar plate to create a lawn of growth.
Did not discard the swab in the waste container.
Moved the disc after it fell on the agar plate.
Placed a disc too close to the edge of the plate so it was not possible to measure the diameter of the zone of inhibition.
Dropped a disc on the benchtop.
Did not space the discs evenly on the agar plate.

[@Old_Guard2]

Thanks from the future, you are a life-saver

[@livybearful]

I love you

[@juandiegofajardohernandez5369]

@@livybearful omg, thank you! HAHA

[@afnansyifamuhammad9016]

OMG, Thanks So Much

[@vanessaestrela1050]

You saved me!!! thank you so much!

[@therubinlab]

I like the idea of including food colouring, that's a great teaching trick to show the concentration gradient! I think my students sometimes struggle with visualizing this with a verbal explanation alone.

[@nourserag5991]

WOW the experiment now for me seems so easy after watching every step of it carefully and also the brief summary in the end made it even more illustratable to one's mind .thank you

[@iqdefinition3253]

Thanks for not rushing through but putting it in simple and understandable terms. And that yellow glove scientist I found humorous though important as these are mistakes we could make but now we are alerted to be careful. thank you again and please keep helping students like us.

[@mitra2028]

Are antibiotic resistance test diagnosed by doing Urine for C/s test?

[@SavePaws_FriendlyWorld]

Best educational Concept Clarity.... Ur nature of Guiding is Excellent 👌 Now I can Study, rather than boring my Nuisance lectures

[@manaseetelaang328]

This made the concept incredibly clear, thank you!!

[@navaneeth_us]

Top-notch conceptual correlation and beautifully crafted and video. Thank you so much.

[@adrianamathai7481]

This was so helpful! Thank you!

[@yogeshlakshman8488]

You explained it like a movie mam
It is so awesome hoping more videos
Thank you so much❤️

[@asifkhanrahime445]

I like your way of teaching please keep doing all these stuff about microbiology.....
And if you have time please make some videos about its full details...........
THANK YOU SO MUCH

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@samassefa8579]

Great video. Super clear explanation!

[@poosichomper]

I'm learning this at school, For science and I find this very interesting!

[@adammajaliwa3125]

U 're so amazing madam 🙌 u make it short but very much clear and educative too 🙏🙏

[@SavePaws_FriendlyWorld]

U r just amazing.... crystal clear Concept. Thanks a lot madam🥰 amazing video

[@shalleey]

Thank you!❤️

[@manikankana2553]

That's one engaging and informative video. Thank you.

[@sunandinisingh589]

Thank you sooo much! I could not visualize this by reading from my book!

[@shresthamukherjee6071]

So helpful 💯 Thank you so much

[@prettysavage2203]

So beautifully addressed this lecture ❤😊

[@dayanacheong901]

what is the name for the antimicrobial disc with the code W 2.5 ?

[@azizaabdumuminova3135]

Great and helpful video 👍

[@RedRose-gw9bt]

I absolutely love your channel, but what type of bacteria did you use for this lab?

[@chrissychris9967]

Thank you

[@liketylickchick]

Thank you for doing this video!
I think you're an excellent teacher, also very pretty!

[@anisahmad2714]

Thanks for the video!

[@endymion_hyp]

What dye was used to show the diffusion zone?

[@erickemanueli898]

this was fabulous

[@daryaissa3191]

Thank u so helpful ❤️

[@moh19931000]

Do we have to do this in a laminar flow hood?

[@gabrielrogerio7128]

muito obrigado!!

[@pauldradre7169]

Thank you for your video

[@sankimseng6094]

This so useful and help me a lot
Thank!

[@aboodameri2145]

Thank you Soooo much

[@rafiaakhter2214]

what are the aseptic techniques that were used

[@malakilani6283]

that was amazing, thank you!

[@komlalebu8333]

thank you so much

[@user-tj6rf5tr7r]

Очень полезное видео, спасибо большое!

[@naimanasrin7073]

Thank you sooo much!!!!

[@dr.umheart2388]

Thank you so much

[@matthiaseckert7344]

Very nicely done

[@roselycullen9071]

Thank you it's quite interesting and helpful

[@ujumiriam9174]

Educative, thanks for this

[@kmyachan9795]

Thank you very much

[@user-ch9ng3no9m]

Thank you bae 😍

[@asifkhanrahime445]

Thank u

[@aichamni3543]

I'm just a biggenner and my English is not very good but I understand this video it was so helpful thanks a lot 😘😘

[@Keepstudying-ex1vw]

I have one quaries ,i have isolated endophytic bacteria from plant ,then pure culture ,and i want to test my isolated strain on pathogenic bacteria by MIC Method,but i dont know how to perform it.

[@45bounty]

perfect..thks so much

[@mltlecturesbyTT83]

Excellent

[@RedRose-gw9bt]

I'm looking for a lab idea (fast) and I was wondering if E.coli bacteria was used for this lab?

[@abdixakiinmukhtarcmm116]

I very interesting the way u explain thnkz

[@tomasescribanoselak4334]

Awesome!

[@jericoramirez-ac5397]

What is likely to happen if antibiotic discs are placed on the inoculated media and left at room temperature for an hour before incubation?

[@zakariazaki7513]

I like this video keep going 🤠 greeting from Morocco

[@nonyequeto8406]

Whats the method of inoculation? Thank u

[@user-wz8he9lx8i]

تشکر .مرسی.tankeyou

[@yuniarelfira5793]

thanks for the video. But, i want to ask something about the preparation of inoculum. I read the protocol of Kirby-Bauer Disk Diffusion Susceptibility and it said that the preparation mus be followed by 0.5 McFarland standard. It means we have to check the density of our inoculum according to 0.5 McFarland standard. On your video, you just pick one colony from the agar then spred it with the sterile swab. According to your opinion, which one is better for the preparation of inoculum? And how the differences between them? thank you

[@divyanshumishra4802]

yeah...I am agree to you...

[@tozfkfkvhv6053]

Good morning great information sister

[@user-cx5in7pi5c]

Heat, measurable scale, reference table, sterilization

[@nurulhidayah-ls6rc]

Great and helpful.. thank you

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@bilalahmad6102]

thnks ma'am.

[@aya.h8846]

What about the 9 mistakes?

[@Peace-fo1vx]

thanks a lot

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@idrisadamu5133]

Very interesting.

[@tatendachawanda6129]

Well Explained

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@axlaamali7560]

great d helpful thank you

[@bangashkpk7914]

thanks madam

[@MD-bf2ce]

I'm very new to Microbiology, but just about flipped when yellow grabbed the sterile cotton tip by the tip! Ahh!

[@innocentabae1118]

Beautiful 🥰🥰

[@divya821]

Mam provide more like multiculture on different samples on single media

[@Az.Beauty.by.C]

what if a disc does not have a halo over it ? does it mean it is resistant to the antibiotic?

[@pinkeupanda6265]

yes

[@aarani4655]

Thank you so much, really helped with my homework! :)

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@krmanjahmad659]

thanks for this type of vedio
i love it.

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@krmanjahmad659]

@@hassanalaa2112
@Hassan Alaa
yea
1.labeling is wrong, with line writing.
2. contamination of swab.
3.wrong in striking of plate.
4. mistake in puting antibiotics in target place.
5. not handling of cup plate.
6. mistake in taking one colony.
7. forgeting small dot(.) on swab
8.upside down of plate that cause differentiation .
9. not adding another same antibiotic on plate.
10. not taking care of safety (like not putting swab in waste container).

[@oulythorng3922]

How to prepare the antibiotic dics?

[@alakwahid]

Do you use tetracycline in different dosage for six time??
Or different antibiotics for six time??
Someone explain me

[@drhumajawed7536]

She used 6 different antibiotic, but here we can use one antibiotic with six different concentration

[@hassanalaa2112]

@@drhumajawed7536
do you know what are the 9 mistakes ??

[@sam_khan_1995]

Thans

[@velusamypothiraj2300]

In result they mension "sensitive" what it is means

[@humeeayisha3199]

Good job 👏

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@humeeayisha3199]

@@hassanalaa2112 no please
Tell me what they are

[@stellafuego]

Hi! I'm just a newbie. Just wondering how can I use garlic or other natural antibiotics for antimicrobial susceptibility test? I hope someone would answer.

[@mostafijurrahmanalhadi5292]

You need to extract the juice from garlic or other natural sources, storing them in ethanol. Make a disc soaked with those extract at different conc. (e. g. 30/60/90 microL or as you want). After preparing the discs with those natural extract, simply follow this spreading method and put your discs on the plate. Search for papers or articles on this.

[@stellafuego]

@@mostafijurrahmanalhadi5292 thanksssss

[@bsparks4211]

I like your question , impressive

[@FunnyMoments-qh6lx]

@@mostafijurrahmanalhadi5292 Men I had the same question and you answer it perfectly

[@nargizaraximova762]

It is interesting Video thanks,😇

[@jennie-jen8138]

Great video.

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@sokefunful]

Narrator: Label the bottom of the plate
Yellow gloves: how about no...

[@zeenatullah9960]

Gr8 madom

[@sivaprasadaraoboppudi4804]

procedure explain in english

[@shalugoutam1763]

So nice

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@buhariabdullahi3128]

Biochemical test

[@user-ve8yt7dy6e]

هذا شرح يخبل وشغل مفصل مو مثلنا كضينا كل أسبوع تأجل الشغل عالورا 🚶‍♂️💔

[@naomiyorgba2420]

Pls make more video

[@bauhiniafolia9673]

Wait, how about the 9 mistakes?

[@kimberlycalip7981]

Same,

[@jakechandler5702]

Why is she hating on scientists who wear yellow gloves? -_-

[@olduglyentwife5700]

They have messy handwriting

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@khalafalasad3209]

Nice

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@dallonpihema16]

Thanks exam passed

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@A000a97]

@@hassanalaa2112
I found 6 mistake
1 It is mistake when picking the coloni
2 Contamination of swab cotton
3 false way to distribute
4 don't put swab cotton in recyclbin
5 antibiotic fall from forceps
6 growth of bacteria not true

[@medicalknowledge7950]

👍🏽

[@unknownuser8297]

What are the 9 mistakes?

[@moonlight950]

I also want to know the 6 errors in the video

[@BrittanyTeague12398]

There are a total of 9 mistakes. If you actually watch the video, you will see the mistakes. She basically points them out for you.

[@pigeonlove]

Stooopid

[@hassanalaa2112]

@@BrittanyTeague12398 do you know what are the 9 mistakes ?? just say it briefly... p.s: i spoted 6 mistakes

[@mustafarahi8670]

Ur mom

[@zahraafakih7020]

To grow the bacteria no meed for a specific medium right? Just wonderning

[@kamalkaperera3599]

D diomand Test

[@heatherwelberry1]

Why in the world would you tell us there would be 9 mistakes and then not tell us what they were so we could see if we got the right one s?? That's actually cruel, you know !!!

[@abilizard]

quite funny

[@birsesvar347]

👍selamun aleykum kolay gelsin başarılar dilerim kalıcı üye olalım inşallah iyi gunler

[@mikemiller7231]

Why are they so baffled at doctor offices when you ask for them to do a simple test like this?
Compartmentalized idiots we usually call them now!
We did this in high school.
Now they get frustrated rude and say bs.