How to Make Liquid Culture the Easy Way: A Magical Hack for DIY Mushroom Cultivation
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- เผยแพร่เมื่อ 22 พ.ค. 2024
- Liquid culture is the simplest, easiest, most effective and most affordable way to get started growing your own mushrooms from scratch. This video demonstrates a simple, reliable DIY method to exponentially expand your mycelium from a single liquid culture syringe into many pounds of spawn in just a few short weeks, no flow hood or special skills required.
Packed with lots of helpful tips, tricks and techniques, this in-depth video explains what liquid culture is, why it’s so important, and explores the whole process from start to finish, including recipes and ingredients, sterilization, inoculation, incubation and storage.
This simple hack will help you save money and unlock a whole new level of possibility in your mushroom cultivation journey, allowing you to get better results, beautiful growth, and keep your fungus happy for years to come.
Made with love by Fungaia,
a heart-centered, donation-based mushroom company
www.fungaia.life/
For the best, most reliable and super affordable mushroom
genetics and liquid culture, visit our favorite culture library:
True Blue Genetics
www.TrueBlueGenetics.org/?cou...
Discount code: FUNGAIA
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Supplies List
Donation-Based Gourmet Mushroom Culture Library
www.fungaia.life/shop/species
The best liquid culture on the market:
True Blue Genetics
truebluegenetics.org/?coupon=...
This is the last genetics provider you will ever need!
How to make your own injection port jar lids
• How to Make Injection ...
Synthetic jar filters: Microppose
microppose.com/r?id=992nf2
Discount code FUNGAIA
(Also a great source for monotubs, monotub filters and injection ports.)
Other necessary items:
Good, clean water
Mason jars
Coffee grinder, food processor or magic bullet
Pressure cooker or Instant Pot
Optional items:
Magnetic stir plate & stir bar
Advanced liquid culture method:
• How to Make Liquid Cul...
Fungaia Laminar Flow Hood
www.fungaia.life/shop/flowhood/
Bulk Liquid Culture IV Bags
www.fungaia.life/shop/spawn/?...
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Upcoming videos:
Introduction to Mushroom CultivationHow to Use a Liquid Culture SyringeHow to Grow Mushrooms the Easy WayHow to Make Grain SpawnIntroduction to Sterile Culture Technique
How to Make Agar Media and Pour Petri Dishes
The Basics of Breeding
Storing Genetics and Managing a Mushroom Culture Library
Troubleshooting Contamination: Identification, Prevention and Problem-Solving
Working with Mushroom Grow Bags
Making Substrate for Dung-Loving Mushrooms
Making Substrate for Wood-Loving Mushrooms
Building a Home Lab
The Laminar Flow Hood
The Fungaia Flow Hood Kit
DIY Bulk Pasteurizer
DIY Cold Storage
DIY Humidifiers
DIY Water Filtration
Tyndallization: How to Make Grain Spawn without a Pressure Cooker
Low-Tech Mushroom Cultivation with Cold PasteurizationOutdoor Mushroom Cultivation
The Liquid Culture Revolution: 5 Ways Liquid Culture Will Change the WorldFungaia's Bulk Liquid Culture System
R.E.M.I.S.: The Remarkably Efficient Mass Inoculation SystemMyco-Materials
All content, including music, was created by Paul Lynn. All rights reserved.
© 2024 Alembic Enterprises LLC
Walla Walla, Washington
(Please forgive the video/edit quality, I’m learning!)
0:48 Intro
1:40 Overview
2:35 Sourcing Genetics
3:01 Materials
4:24 Ingredients & Recipe
7:29 Sterilization
9:37 Inoculation
10:26 Incubation
11:14 Storage Tips
12:20 Inoculation Tips
12:56 Verifying Results
13:48 Limitations of the Technique
15:12 Mush love! - วิทยาศาสตร์และเทคโนโลยี
![[UNCUT] ฝันรักห้วงนิทรา | EP.3 (1/4)](http://i.ytimg.com/vi/zVXAFD1OLZg/mqdefault.jpg)
Hey brother, this is from a cultivator who has been growing for about 50 years… a fellow named, “Marc R. Keith… also known as RR…
“Throwing spores into honey or karo has to be the worst possible way to make a liquid culture and I'll be glad when this current fad passes and folks can get back to established techniques. Just inoculate some grains or brf in the standard way and you'll be miles ahead. For those who wish to make LC for inoculation, here's a way to get 100 syringes full of inoculant in two weeks: 1) Inoculate a quart jar of rye berries or wbs with your spores. Upon full colonization, shake the jar to loosen each individual kernel. Be sure
to give it the smell test to make sure it smells like fresh mushrooms. 2) PC a quart of distilled water in a jar with a filter disk or tyvek, etc. In a glovebox or in front of a flowhood, pour 2/3 of the quart jar of sterilized water into your jar of shaken grains. 3) Shake well and then pour the now mycelium rich water back into whatever was left of your jar of sterilized water. Use the jar lid to hold back the grains themselves from pouring out. You now have a full quart of highly concentrated mycelium water that can then be used as is, or diluted two or three times again, and used to fill syringes. Because the grains were shaken first, the mycelium is ripped into shreds and will be sucked through even small needles easily, although larger needles always work better for this. Remember, mycelium grows poorly in water unless under constant stir which oxygenates it. With grains, they colonize in two weeks or less because oxygen is throughout the jar in the spaces between the kernels.
LIQUID CULTURE - A glovebox is not advanced equipment. One can be made free with stuff around the house, so there's no need for people to work in open air or fecal and mold infested bathrooms and kitchens.
There is also no way to generate 100 full syringes of liquid mycelium in very small pieces within two weeks from injecting spores into honey or karo. I'm sure if people blindly used the grain method I described above, many would still have problems too, because they're inserting unproved and possibly contaminated spore solution into grains, which also isn't proper mycological technique. However, agar can easily be poured in a glovebox, and the Petri dishes used to isolate healthy mushroom mycelium away from any contaminants in less time than it takes to even know if a karo LC is contaminated or not. That agar wedge can then be used to inoculate the quart(or pint) jar of grains, that can then be turned into at least 100 syringes, or used for g2g transfer to get ten jars the old standard way. In addition, after the water is poured out of the grain jar, it can be placed back on the shelf to re-colonize, and the process can be repeated again. I just strongly feel new growers are doing themselves a disservice when they simply inject spores into honey water and then sit there waiting for something to happen. At the very least, inject directly to grains so you can visually inspect the process. Perhaps Agar's lids with injection ports will help those who have never used grains to get off to a good start.”
"here on planet Arrakis..." instantly got my like LOL
Ohhhhh I like you said it's about having a deeper relationship with our world, this is something about more than human!
Another great video, I dig the intro it elevates the feel of your content.
I can really see your love for mycology!! Thanks for making detailed and high quality content!!
Glad to see new activity from you. On behalf of all of us that recognize the skill, knowledge and talent you possess, I would like to say thank you.
I like this guy, and he makes good videos, but all you really need is water and a source of glucose.
Some of the things he suggests, like nutritional yeast, may hasten colonization of your liquid culture. However, it may also potentially increase your tam rate by enhancing bacteria survival and stimulating its growth. I find its best to keep it simple, by using only water and a source of glucose. I prefer a mixture of ELME (Extra Light Malt Extract) and raw unprocessed sugar (my preference is turbinado sugar). This helps to lower contaminations from outpacing your mycelial growth.
I do, however, like his suggestion of adding a bit of what you plan on feeding the mycelium to the LC, as it seems to "train" the fungi to know ahead of time what its going to feed on, instead of having to adapt to its next food source. This helps hasten the colonization of your grains which can not only save a bit of time, but also help the mycelium outpace any contaminants it may meet during inoculation or endospores hidden within poorly prepared grains.
Btw, that jar of "Donkey Shit" had me rolling with laughter 🤣
Hey, I Definitely agree with your comment. One quick question tho. I've heard about soy peptone from quite a few people. What are your thoughts on a pinch of that in the mix
@@TheDarkArtist66 I would suggest against peptone. It only speeds up the LC by like an average of 2 days. Its much like yeast in application. Many add both peptone and yeast to "supercharge" the LC broth. However, in my experience it doesnt hasten growth any more than one of those additives alone. Personally, I would rather wait the extra 2 days to lessen my probability my jar tams out. If its one thing this hobby has taught me over the last 2 decades is patience is a virtue. Id rather wait 2 days extra then lose out on an entire batch of LC, and the grains, sub, bags, money, time, effort, et al that follow. In the long run, I end up with more fruit due to no loss, and only had to wait 2 extra days for it
@WhiteBeardHashtag1205 thanks for the advice. I'm fairly new to this whole thing although it's something I've always wanted to do. I guess it was never the right time. Maybe it's not now but you only live once and might as well make the trip thru it worth it lol. Thanks again bro
Love your energy!
Looking good Paul!
Good stuff! Thanks for making content!
Very helpful. Thank you!
Thank you…freshman at this school! 👍❤️💯🍄🙏
I love learning about innovative new ways to mix up LCs, excited for this one!
Nice video, def gonna subscribe
Very good channel, congrats man
Glorious
Thanks Paul. God bless 🙏🏽
Thank you.
I dig your style and have binged all your videos. Been subscribe for a while now so thanks for all your content. Great stuff Paul
Hey man just found your stuff but I'm gonna sub at first sight
Thank you so much for your incredibly informative and educational videos, you are awesome, I am learning so so much. Looking forward to my first LCs arriving soon.
Many thaks Master !!
hello fellow walla wallan mycologist....glad to have a resource so close to home
Thank you super man 👍
I appreciate the mushie info, its what i came to see. Ill subscribe.
Its really great to meet you (being another member of the purple dinosaur association)
Thanks!
I love your videos! I had some contamination issues in my first LC attempts and hadn’t thought about the condensation issue! I’m sure mine happened because of letting the filter get wet. D’oh! I’m in Seattle so we do start with some pretty good water.
I’ll also try some additional nutrients. I like the idea of acclimating the mycelium to the material it’s going to be growing on.
I started with some of the magical fungi but really want to try oysters and especially reishi, if for no other reason than they’re so gorgeous!
Great video. Love the personality and you have such a great way of educating in an entertaining way. I look forward to more videos like this! I prefer a more simple .5% - 1% LME ratio nutritional yeast makes a great addition to this. I will definitely try to start adding some grain of choice as well.
Also i can absolutely vouch for true blue genetics, especially for some of your more "interesting" varieties. Always clean and vigorous cultures. I even had some cultures that i left alone for over a year, put it to agar and it came to life without issues.
Enlightening
Thanks for the great info! With the hair and the vest I like to imagine you were a boyscout who got seperated from the group and spent the next 10 years surviving in the woods on mushrooms.
Finally something else besides that d@mn blender video. Bet I started watching that thing a hundred times now hoping it was a new video. Anyways thanks for your contribution to furthering others know how.
For water choice: I completely agree with Paul's assessment as far as avoiding town tap water. I ran an experiment where I produced two LC's in mini jars using 5ml of viable LC each, one with distilled water and one with tap water as solvent for corn syrup. I had a negative control where no syrup was added to the water (spores still added).
Results: Tap water's growth was hindered by about 1.5-2 days.
This difference is negligible and hard to notice after growth starts to take off exponentially.
*The genetics of your specific variety play a huge role here too, as some mycelium is more tam resistant than others*
Mush love thank you for what you do
I wish I had like a thousand accounts so I could give you a thousand likes
Thank u 🎉
Love the vid. Also, love the bright mushrooms shirt!!! 🍄
Glad to see your back. Hopefully you stick around longer this time 😁 whatever happened with your battery powered Flowhood?
Thanks! I'm here to stay. Aiming for one video per week at least through April.
I've made a few dozen battery-powered flow hoods. Not plugging them much these days because they're a pain to make and when it's all said and done I hardly make a dime on them. A cool thing to offer, but I'm more focused on other projects. Plus a lot of other cheaper FFUs nowadays, not nearly as clean but they work well enough people aren't as interested in real laminar flow hoods anymore.
He’s back
best series if seem you feel very personal about your shrooms try feeding them sound frequency at different levels they do talk as we see might find they talk back and bloom
Quality content and excellent presentation. You know when you have an idea to do something, maybe like make educational mycology videos that are insightful and articulate, but then someone else does it so much better than you could do it, you’re just like never mind.. is there a name for that phenomenon?
Believe in yourself and just do it my man
Thanks my friend. Don't sell yourself short, there's always room for more mushroom-related youtubery!
Wow he answers
I love your videos. Very clear informational. I do have one question. Do you keep those jars on the stir plate the whole time the myc is growing? Or do you juat set it on the stir plate for like 10 minutes every day or so?
Yes, I stir constantly. I find this accelerates growth, prevents surface growth and clumping and keeps the oxygen levels high.
I heard about True blue genetics from watching dudes videos! TBG is the ONLY vendor I acquire my liquid culture syringes from. I will not go through anybody else anymore. Fast shipping, clean cultures! Huge selection. I won’t stop until I have their whole library. They also hook up a free random strain with every order I have placed so far
paid?
You sent me a beautiful little pamphlet last year with some thoughts that I really appreciated and loved, but have since misplaced. Is there any chance I can obtain another copy, even if just electronically?
Can I use a spore syringe for this too?
Hi! Can you do this same recipe with a spore syringe?
While LC is preferable, will this work with spores in syringes?
Is the distilled or RO filtered water good for LC?
15:11 ❤
😂😂😂
whats the exact description of the stainless steel carraige bolts
Great video. Do you have any idea if protein as a food source any good for fungi in general? Do they have the enzymes to decompose the material? I've seen a youtube short where a guy inoculated a whole egg with cordiceps so i'm kinda confused because agar is used instead gelatin for petri dishes.
I don't know much about the role protein plays in various fungal diets, but mushrooms contain very high levels of protein, and the grains and many of the substrate supplements commonly used throughout the mushroom cultivation industry have high protein content. Cordyceps are a little different than most of the other varieties we would want to grow because they eat insects. The egg experiment would not work for most wood-loving species.
@@_fungaia Thank your for the response. I think when i'll have more experience i'll try out different kind of proteins/peptides to see if they affect something. I know peptones work but they are rather expensive and hard to come by. Yeah i had a feeling cordiceps is a little different so that's why that egg experiment works. Have a nice day.
As someone new to this field I haven’t understood all requirements used in the process most especially what he crashed as in blended
As somebody with 40+ years of experince in cultivation i can say that you are making this entire process WAY more difficult than it needs to be .
1. Chlorine in the water is a non issue , chlorine evaporates readily and assuming your jars are properly setup , all chlorine will be gone within 24 hrs of autoclaving .
2 . As far as contamination growing through the filter , this goes back to how your jars are setup . If you use any sort of filter disc , micropore tape or even polyfill , just the vapors alone are enough to saturate the filter media to the point where contams can grow through the filter , to prevent this you should use a hydrophobic (not hydrophilic) syringe filter for ventilation (preferably 2 microns) . A hydrophilic filter will absorb vapors from the culture media and allow contams to grow through . A hydrophobic syringe filter is resistant to being saturated by vapors or liquid lc media and therefore resistant to allowing contams to go through .
3. As far as lc recipie goes the clearer and simpler it is the better , a clearer lc media will make it much easier to spot infection . Clear corn syrup , a dash of lme and a tiny pinch of peptone is all thats required and will result in an almost water clear lc . Just my 2c worth
Thanks for your input! Yes, there are many different methods and supplies that can work.
We each have our fixations. Water quality and nutrition are two of mine. True, we can live for a while on corn syrup and tap water, but I prefer to eat better, and I believe our fungi do, too!
He also said to "use a lot of liquid culture". I say No don't. It will over saturate the grains with too much water and will most likely cause a stall or complete failure.
He would certainly cringe at Home Mycology 's videos as he USES K.I.S.S
@@TheTubejunkyhome mycology is a no BS channel.
yes he's eccentric in his own way.
simplified and effective
Question: My first Mono Tub has just been harvested for the first flush.. assuming I get another maybe a couple more flushes, why couldn't I just take some of the substrate that's left, and mix in with new substrate? Skip the grain step? How big is Contamination if the first spawn cake was pure? Is it possible to keep an initial spawn going.?
can I go from spores directly to liquid culture?
Very informative and well explained. I'm looking forward to beginning my voyage into mushroom cultivation. Thanks Paul & Cheers from Stanwood, WA 🍻
Does anyone have a translation to instapot settings?
I like the "Donkey Shit" label on the 😅
Looks like somone is making homemade bombs in Death Wish 3. Love it!
Why do you prefer regular mouth jars/lids instead of wide mouthed? I just got materials to make lids but they are wide mouthed
It really doesn't matter. I like regular mouth for LC and wide mouth for grain. The smaller opening is just less surface area, which means slightly less exposure risk. But it's a small difference, and either will work fine!
I've been running pints 90 minutes in an instapot for a while, I've had random contamination I usually see on a single grain in a spawn jar. I put a thermometer in a jar as soon as it came out last time and I was only at 220. What's your opinion on time and temperature I would be aiming for ?
Instant Pot supposedly gets up to 240, which is plenty for the vast majority of pathogens. The second you vent the steam, the temp will drop rapidly, so the thermometer probably isn't giving an accurate reading. You can always up it to 2 hours if you really think that's the issue. My guess is a lid filter failure is as likely as inadequate sterilization.
I haven't used an instant pot, though I want to try one out for one of my next videos. The only other thing I wonder about is if it properly vents the air from the pressure cooker before building steam. Only pure steam will approach the adequate temperatures at maximum operating pressure.
Hey bro enjoy the vid, still watching. What mushroom would you personally use if you had colon cancer? And liver cancer? 🙏 My friend is ill, he has some turkey tail Ohm brand stuff, i told him he should probably be taking other mushroom species, reishi or cordiceps. I really wonder about chada and oxolates if a person had rough kidneys and cancer lol geeze . This wont be taken as medical advice do to Marianette culture lol thank you , give dog a treat
Sorry to hear that! Medical questions are above my pay grade but it sounds like you're on the right track!
You say the final jar of LC will store in the fridge for a very, very long time. How long exactly?
It depends. Some strains last longer than others. In general, wood lovers hold up better than dung lovers. If you seal it completely or fill syringes like I suggest, they can last years. It's a matter of degrees. I find that the overall success rate drops off at about 6 months, and again at about 2 years. But I've had success reinvigorating culture that was 5+ years old! I'll be talking a lot more about this in my upcoming video on managing a culture library.
@@_fungaia The video is quite interesting, but there is a problem when removing hot jars after sterilization and tightening the lids. In the syringe filter, a lot of condensation forms if it is not dried under a stream of hot air. We get сontamination has been confirmed, through experience.
I look for a lot of information about liquid cultures, and I keep track of new materials on this topic and communicate with experts. I also conduct my own experiments.
I want to confirm that the shelf life of such crops is more than 2 years. As for the composition, I use the ingredients that are convenient for me And what the mycelium should process in the future. In short, the base is a decoction of sprouted wheat (3 days after it has been in water for 1 day), straw broth (proportion according to the colour of the tea), a decoction of wood chips, 2 tablespoons of honey, сhloramphenicol tablet - 250 ml (in my case, it is half a tablet per 3 litres of liquid.
All my cultures were kept indoors at room temperature. Once a month, or sometimes every 3 months, I put them on a magnetic stirrer to break up and oxygenate them.
I like this method of preservation very much, and recently I have been inspecting the cultures, taking samples from the jar and also taking a syringe that I have been using since the jar was created. I sowed them immediately on a grain bag, and they are growing well. (My crops are all oysters and wood-destroying mushrooms)
I like people who share information, greetings from Ukraine
I currently colonize 2kg of wheat spawn in 8 days thanks to inoculating large amount
What would be the insta pot guidelines?
Stay tuned for a pressure cooker / sterilization video!
Why do some button mushroom seed mycelium grow slowly?
Hi, corn syrup isn’t easy to come by in Aus. Was wondering if a malt/destrose/corn syrup powder used for brewing beer is a suitable mix for making a liquid culture?
Dextrose
Yes, light, dry malt extract powder is my preferred sugar. I don't recommend using corn syrup anyway.
wow that is a crazy recipe. 600 ml distilled water, 18 grams corn syrup, 1.2 grams lme, 1 gram peptone.
What's up with the buckets? If you don't mind me asking.
Haha I wondered if anyone would ask. I also run a small-batch kombucha company. Those are my SCOBY babies.
@@_fungaiaThought they were some new-tech oyster experiment.
will chicken poop, or dirt with a heavy amount of chicken poop in it, i.e. from my chicken run area, work well for dung loving mushrooms?
You can, but only in extremely small quantities (2-3%). It has 3 or 4 times the nitrogen of horse manure. It should be aged and dry, and you'll probably want to add some pH buffer as chicken poop is usually acidic.
@@_fungaia thank you. i've been wandering that for a while
👍👍🤝
I keep getting contam of bacteria in lc, any tips to overcome it??
The first thing to check is your filter. I recommend microppose filters:
microppose.com/r?id=992nf2
The most common contamination vector is during inoculation. Stay tuned for a video on "Sterile Culture Basics" coming out next Thursday!
15:12 😂😂😂😂
LC mix shouldn’t be cloudy before you inoculate it. It should be crystal clear
It depends on the recipe. I like to add a lot to the mix, but I know a lot of folks prefer clear LC. Follow your heart!
its been stated and makes sense- that dung loving mushrooms dont like dung-- butthey do like the nutrients in it - they like dung as a dog likes a bone only because he cant get the steak that was attached. the spores are eaten and get mixed with the grains and grasses and get deposited in dung... so in reality-- do we really need dung or just the cud-- a steamy pile of grass and grains.. what is your thoughts?
Great question! I like the metaphor. I think one of the main things they like about the poop is all the microbial life it harbors. Chytrids, bacteria, protozoa... the stomachs of grazing animals are quite fascinating ecosystems. This is why, in my experience, pasteurization is so much more beneficial than sterilization, and why dung lovers don't respond well to the manure of grain-fed livestock. I think it's less the grains and grasses they're after, and more the banquet afforded by the various biochemical products of all that microbial activity. It's the poop within the poop!
Why would there be chlorine in tap water. isn't it toxic
It sure is! But all tap water is treated with chemicals to sanitize it and prevent pathogens from growing in the pipes. While this is very effective at maintaining sanitary water systems, chlorine, bromine, fluoride and all the other chemicals used by most water districts are not only toxic, they also react with other compounds in the pipes and water to create even nastier byproducts. I think it's really important to filter your drinking water! I'm making a video soon on DIY water filters.
Making liquid culture the "easy way" should take like 30 seconds to explain, self healing injection port lid no fae hole in lid fill quart jar with water and one teaspoon of honey pc for 30 min let cool and inoculate DONE
City water or ?
Gypsum, wheat flour, honey, sugar, yeast, corn syrup, peptone or malt extract. All fine, the donkey shit will not benefit most species I guess?
I pray to God that you're not a socialist or communism supporter. I've seen a shocking amount of people that get into mycology that supports these ideologies that are murderous throughout history even today. I don't get how someone can take these powerful substances and not see the evil within those systems and uncover the truth.
really now
@@jhmalan6000 Yes, really. The stitching on his jacket with the phases of the moon, I see this in the rooms of a lot of these extreme leftist types and I've noticed that more and more of the mushroom world is getting inundated with socialist supporters which is terrifying. These substances are supposed to help you uncover the truth and be more connected with nature and yourselves. It makes no sense why someone would take these substances and still support an ideology that murders people and strips freedom away and isn't grounded in objective reality.
@@jhmalan6000 Look at these psychedelic festivals, people dressing in scanty clothing, some even dressing as demons or the devil and promoting leftism and hating on religion. It makes no sense for someone to take these substances and hate God or not believe in God. People that have no had a religious experience on these substances are either not taking enough out of fear of their terrible actions and behaviors to others and themselves being revealed to themselves through the trip or refuse to believe that a powerful experience they had was religious and instead write it off as a hallucination, which is convenient.
The Fox News boomer energy here is way out of left field 😂
I think it's unlikely.
(If you are schizophrenic, you should probably avoid magic mushrooms.)
THANK YOU PAUL, FOR ALL OF YOUR GREAT VIDEOS. NICE LC VIDEO YOU GOT HERE, I THOROUGHLY ENJOYED IT. KEEP UP THE GREAT WORK MY FRIEND. =)🍄🤍👍
Thank you.