My New Favorite Liquid Culture Recipe for Growing Mushrooms
ฝัง
- เผยแพร่เมื่อ 5 มิ.ย. 2024
- In this video, I'm excited to share with you my new favorite liquid culture recipe for growing mushrooms. It's a simple yet effective technique that results in a clear solution and promotes faster and more consistent mycelium growth. By combining just 1/64th teaspoon of peptone, 1/64th teaspoon of light malt extract, and 1/2 tablespoon of corn syrup or honey with 600 ml of hot distilled water, you'll be amazed at the results. Join me as we explore this recipe together and unlock the potential for incredible mushroom cultivation. Let's dive in and discover the secrets behind this game-changing liquid culture recipe!
Start your mushroom cultivation journey with 25 diverse varieties perfect for pairing with this recipe, available on my website : www.sporensprout.com/
Modified Jar Lid Tutorial: • How to Make Self Heali...
Recommended Products (Affiliate Links):
Peptone: amzn.to/3rk1wmQ
Light Malt Extract: amzn.to/44roYgi
Corn Syrup: amzn.to/43cyOSj
1/64th measuring spoon: amzn.to/3XBGqN5
Handheld mixer: amzn.to/43l6cXe
Magnetic stir bars: amzn.to/3JLbS5U
Magnetic stir plate: amzn.to/3XIKBXh
Join the new Mushroom Cultivation Discord server! / discord
#mushrooms #growyourown - แนวปฏิบัติและการใช้ชีวิต
![[Full Episode] MasterChef Junior Thailand มาสเตอร์เชฟ จูเนียร์ ประเทศไทย Season 3 Episode 1](http://i.ytimg.com/vi/Qs5zdBqLn_8/mqdefault.jpg)
Funny story...been watching your videos and ordered all the stuff in this one so that I can start growing my own liquid culture. I just went on etsy and randomly selected a liquid culture syringe to get me started. I didn't even look at who the seller was until I had sent the payment. Turns out it was you XD
That is funny! 😂 Thank you and good luck with your experiments! 🧫 🍄
Thank you brother! Peace and love☮️❤️🙏🏻
I've been using that same recipe for a couple of years now. It's the best imo. That peptone really makes the mycelium POP.
I love how this comment section is basically a huge list of FAQs *that are being answered!*
Keep it up, man. You've earned another subscriber.
Been looking for this recipe, i believe this is the recipe used in the premade jars i bought.
Your results are fantastic looking, and I LOVE the simplicity of your recipe. Most I have read and tried are far overly complicated in comparison. I can't wait to try this out! Cheers!!
Feel free to add more ingredients, you can use 1 gram of LME and .5 grams peptone for stronger solution. Just make sure there is enough water in the pressure cooker so that the water level is touching the bottom 2 inches of the jars while pressure cooking. I used low amount of ingredients because I kept getting sediment but found that I wasn’t using enough water in the pressure cooker which caused the sediment.
I stuck spores from a MSS into coconut water as an experiment and it started growing myc after 4 days.
I've been using this recipe for a out 6 months now, i use a little more peptone and malt extract but it looked like we get the same kind of results, as soon as you showed the ingredients I got excited, this is by far the best LC recipe I've used
It’s really a game changer! I love how clear it is and how fast the mycelium grows compared to just honey. Thanks for watching and Good luck !🍄
Hands down the magic sauce
@@SporenSproutWhat's your process on growing indoor chicken of the woods?
@@Irish0wl that’s my question as well. So tasty!
I once stored it in cold cooler and it ruined my LC. It wouldnt grow anything anymore.
Can I just keep it in a kitchen cupboard for a few months? Did that only take you 2 weeks to have that much mycelium from just 1 cc of LC injected in that jar?
Spore n' Sprout is a great place to learn about fungi growing!🙂
Thanks!
Amazing ❤
Thanks for the easiest to follow video on liquid culture on youtube. Everyone else makes it super difficult. Or maybe I'm just not a smart fella?! 🤷 lol 😆
this video was cristal clear! ;D
Thank you for your videos! They are very educational.
I am more familiar with your old videos. Trying to catch up now. I feel my knowledge is a bit rusty after over a year break from studying mycology. I had experimented little and due to health conditions I was forced to have a break and my LC spoiled meanwhile.
I’m ready to restart but now all I have is 1 spore print and 5ml spores suspended on water. Unfortunately I am not able to get more.
I have materials for grains on jars, bags, agar, LC, bulk substrate… What would you recommend I start with so I make the best of the little I have? For grains I have access to rye and popcorn by the way. I am interested in starting a good self sustaining cycle loop so I don’t waste the spores I have.
Your help would be greatly appreciated. Thank you in advance!
I have been looking and looking for a product to buy or recipe for Water Clear Liquid Culture, not yellow... Its just a preference !! So Thank You !! This is the only one I found so far !!
I’m glad it helped! Thank you for watching! 🍄
That is awesome I'm going to try it
It’s a game changer!
Thanks for the info
First of all, thank you for sharing all your knowledge! If a person wanted to run an exhaust fan a few times an hour for short periods of time, do you have any recommendations as far as a timer that is that precise? Thank you again!!
After trying the original recipe with a 64th of peptone and LME and karo I just put together another 7 quarts of the suggested recipe for more nutrition. The first way shown in this video worked very well. I simply want growth for another round as fast as i can get it and plenty of nutrition to fill the jar with mycelium. The suggested recipe was 1G peptone, 1G LME and half tbsp of honey or corn syrup. I mixed the first quart that way and while stirring it i noticed it was definitely not completely clear. i lowered the LME to between.5 and .75 if a gram, not measuring it exactly just leaving the 1\4 teaspoon less than full and that solved the problem if the slight haze.
Absolutely clear LC with that recipe. 1G peptone which is 1\4 teaspoon, .5 to .75G LME, light malt extract and half tbsp 7ml of Karo. I used karo only because that is what was there and honey seems to caramelize when i use it at 4% I have not tried it at lower concentrations, it might be perfectly fine.
I wanted to list my findings for others who are trying to get completely clear LC but still wanting high nutrition.
Thanks a million for all your help, especially the trick with adding 2 inches of water covering the jars in the bottom of the all American pressure cooker so I don't cause a haze after sterilization. It worked perfectly. I ran it between 12 and 13 psi after getting it to that pressure for 35 to 40 minutes and immediately vented. Perfect results.
Thank you very much!
Thank you for making this video, have not tried it yet, but curious how to get the lc out of the jars to inoculate other thing! Thanks inadvance!
You could use this method I designed: Filling Mushroom Syringes with a Peristaltic Pump System
th-cam.com/video/zMVBLV5Cdxo/w-d-xo.html
Or just tilt the jar and suck it out with a syringe
I use this same recipe…it’s the bomb. One gram of each to 600ml water 👍🏼
How is it possible that so little amount of sugar works far better than let's say 4% ?
The Myc actually runs for nutes, instead of being surrounded by tons of sugar. Think it helps expand better @@PPP-on3vl
Do you realise you use about 20 times the amount of what he shows in the video??
@@nelisv7218 nonsense. Other growers verified & I got the recipe from an industrial grower. Had spectacular results & no contam.
Because most sugars like corn or honey you would use at a 4% solution. LME is a complex carbohydrate and can be used at far lower concentrations such as in this video
The video is very easy to follow... thank you bro, from your guidance I made it. As a result, I get mycelium that floats lightly, so clean and smooth like a cloud in a solution that remains clear until the end of the process. Unfortunately I don't understand how to share with friends here. once again, I thank you very much..
No problem! Thanks for your feedback 🙏 I’m glad you like how clear and fluffy the mycelium is with this method.
12 months!? Wow... trying this immediately
LME is the best IMO! Clear and fast growth!
What flow hood are you using? Does it work well?
I got your spores in the mail super fast, thanks. I'm about to make these mixes today, and eventually use them to mass produce shrooms in my 2400sqft shop. Let's goooooo
Nothing better than that 🙌🏼 Good luck brother! 🍄
Thanks for the valuable content. What amount of dextrose can be added instead of corn syrup?
1/2 tablespoon of dextrose
I have of the ingredients except for malt extract, amy other ingredients to sup the malt? Amd ty
So the idea is to sterilize the f2ck out of the jar and fill it with some liquid carbs? Would you consider trying a really crude version of this? Like wiping down the jar with some off the shelf isopropyl alcohol and then dumping some sugar water in with some innoculant? Idk I think it'd be cool to see if it works. Anyway, really cool footage.
Hi, I appreciate your concise and simple instructions to create liquid culture. What is the suggested time to let the culture grow mycelium or is it by sight? (i.e. 50% of the liquid has mycelium) I'm trying to build up my culture backlog and extend my spore purchases. Thank you!
Yes I’d shoot for 50% and it usually takes 1-2 months
i love the clearness and very fast growth of this recipe. thank you! however, most of my strains now start to make significant rafts. i first thought it was contam, but surely they are not all suddenly contaminated. it gathers up in big floaters on top that is very hard to break. have you experienced the same?
Yes I experience the same, You have to stir frequently to prevent the mycelium from doing that and becoming too thick. You can switch to honey LC if it becomes annoying. Or you can watch my blender video where I blend the LC: th-cam.com/video/LGvAu0ux-VI/w-d-xo.htmlsi=Or-S6wzPFjK0Y62v
@@SporenSprout i was thinking if they get a bit too much nutrition still? have a feeling it could be related. not feeling super comfortable with these rafts, and the additional step of blending i would also like to avoid. what do you reckon? i'm coming from honey LC and that's been working fine, but i feel the clarity of this method makes it easier to spot bacterial contam.
Love this! ❤ But serious question : What kind of needle do you use to get to the bottom of this? Or do you fill the syringes with the lid opened in a sterile environment? Thanks!!
I tilt the jar and use a regular 1.5 inch needle.
Tilt the jar
No u tip the jar or u can get a longer needle but when doing so run risk of letting something bad in and ruining ur culters
It looks like a lava lamp❤
Thank you for this recipe. What do you think about using powdered corn syrup? Does mycelium have a preference of bone peptone over soy, or vice versa?
Do you feel that this broth will work for pestilotiopsis microspora, initially anyway?
We are excited to find out how this works with all of our different species. We have a number of projects going on both professionally and personally that this may be a nice addtion too.
Thanks again for sharing it with is all!
I’d go with soy over bone. The bone peptone makes the mycelium grow on the surface too much and also smells bad. Yes I think it will work great for any microorganisms really. I have never heard of corn syrup powder but for me corn syrup is never strong enough. I like honey.
I wonder even grinding down Morning Glory Petals to absorb in with Culture would help with Potency.
I love morning glories!
I wish you sold these jars on your website !!!! Lol I live with other people and can not make these, plus I do not have a pressure cooker... :(
Hi what an informative video. thanks, I am making mine to your recipe and need to know what temperature to store the jar at?
You can store them at 70-75 F. When mixing the ingredients you should start with room temperature water. The ingredients do not dissolve well in hot water. You can use 1/4 teaspoon (1 gram) of each powder as long as the water is not hot. It should dissolve perfectly in cold/room temp water. It also helps to reduce PSI from 15 to 13-14 to prevent opaque cloudy LC media. Good luck!
@@SporenSprout thank you for your detailed response
I wish I would have seen this video last night. I only saw the short one and didn't catch the statement about watch the full video. I made 7 quarts of the lighter nutrient content. I'll be making this heavier recipe tonight if I can figure out what to do with my all American canner to get it to not make the mixture clouded. I'll definitely take your advice about running it at less than 15 PSI.
Thanks for everything, cheers.
I didn't see this and as usual I mixed everything in hot water, the peptone took a small amount of agitation to get it mixed but all three ingredients were very easy to mix in hot water. LME and I'm using light Karo just because that's what is here. I have honey but at 4% it badly clouded and a lot of sediment. I'm guessing I caramelized it with too much heat and too long of a temperature.
My presto states to fill 3 quarts of water when using it.. does the LC recipe glass mason jar have to sit completely above the water during the 20 minutes or is it ok if the bottom portion of the mason jar is submerged in the water?
An inch or 2 covering the bottom of the jars is normal. You can use jar lid rings to help raise the bottom rack further if needed.
Thanks so much, is it possible to convert the tea and tablespoon measurements into grams per 600ml?
55 mg of each powder and 20 grams of syrup.
@@pouyarezaei5976 Yes, you can try adding more but if it produces sediment than put 55 mg of each.
If I have light spore specs still floating do I keep it in inoculation longer and weight it out ?
Yeah. Next time germinate the spores on agar first.
when it is ready to fruit what do i use for the substrate do i have to use grain and inject it or can i sterilize a plastic bin with cardboard or something else please let me know
Make some grain spawn: How to Make Sterilized Rye Grain Spawn for Growing Mushrooms
th-cam.com/video/uFQr2JhqmG4/w-d-xo.html
What heat and light conditions do you sore the LC jar at immediately after inoculation?
Light doesn’t matter, temp set at 70-75 F
@@SporenSprout Thanks man.
This might be a dumb question but I'm a newbie so please don't make fun of me lol. Is it possible to take liquid culture that has already been formed, and inoculate another jar with it to allow it to grow? Essentially can you use one jar to make more jars?
Also, on a side note, that is some absolutely beautiful looking liquid culture you have. Nice work! 👏👏👏🙌❤️
Yes you only need 1-2 ml of liquid culture to make a whole new jar and you can keep doing that for a while.
I didn't see this and as usual I mixed everything in hot water, the peptone took a small amount of agitation to get it mixed but all three ingredients were very easy to mix in hot water. LME and I'm using light Karo just because that's what is here. I have honey but at 4% it badly clouded and a lot of sediment. I'm guessing I caramelized it with too much heat and too long of a temperature.
Is this better than your honey recipe for Cordyceps Militaris? I ordered some syringes from you last week and plan on making the LC before they get here. Thanks. Love your videos.
It works well but if you don’t stir it everyday the mycelium tends to clump and tangle which can be annoying. I prefer honey LC for that reason.
@@SporenSprout I do have a stir plate if the LC is better suited for propagating the Cordyceps. Thank you!
I had good sterilizing in microwave for abt 5 min
What do you think about adding brewers yeast? Ive seen other recipies with this but im not sure what it does.
Is there a reason you stirred with a frother initially instead of just using the magnetic stir bar?
Doesn’t matter really, just use whatever you have to stir it at the beginning.
great recipe, can i replace syrup with dextrose?
Yes
I have a question . Do you burp your sterilizer , or simply allow it to run for 10 minutes before shutting the valve ?
I just let it vent for 10 minutes and then shut valve
❤❤❤
Would using the mycelium in here be ok to use in an extract? Instead of like using the mycelium from rice?
Yes this is a great way to harvest mycelium without grain filler.
I've been using a recipe that uses about 1/2 tsp peptone and 1/4 tsp LME, but the same amount of corn syrup and water. You've found these much smaller amounts of peptone and LME to be superior to larger amounts like that?
Yes in my experience the smaller amounts work better. If you can fit more in without turning the solution cloudy than keep doing that. If there is any cloudiness the mycelium will be initially inhibited in my experience. I’m thinking a hot plate will allow you to dissolve more without it compromising clarity.
@@SporenSprout Interesting. I'll definitely try this out.
Is this sterilisation is 30 minutes at 15psi? .should we Weight until the pressure cooker turns to 15 psi and the 30 minutes or all together 30 minutes from the minutes I started pressure cooking?
Yes 30 mins once it gets to 15 psi.
Great video, like always!
Is there a substitute for the peptone? I wanna try this recipe but in my country is not that easy/cheap to get!
Beef extract or vegetable extract.
@SporenSprout oh you can use beef extract? That's really cheap and I have it in my pantry. Peptone is ridiculously expensive in tye UK but beef extract is like 3 pounds
hydrolyzed collagen bovine unflavored
Would vegemite. Work?
@@SporenSprout if there is much salt in it, is that a problem?
Karo vs corn syrup ? Or what’s the difference ? Thanks in advance love the videos !
Not much different other than the karo most of the time has vanilla flavor added to it
I read the ingredients and saw both types of Karo have vanilla. I wondered if that made any difference at all. I was concerned it might caramelize but I had no issue.
Hey amazing video!!! Quick question: does this recipe works with lion's mane mushrooms?
Yes it works for all species
@@SporenSprout can I replace malt extract with malt extract agar?
@@hungnamnguyen9003 no because it will become a solid instead of liquid due to the agar.
@@SporenSproutThank you so much for relying to me!! But is there a catch for this method? Is there a limited time you can do this?
I also watched your video on grain to grain transfer. Is there a limited times you can do that also?
@@hungnamnguyen9003 you certainly can’t do it forever. After 1-2 years you will need to start again from spores which is pretty easy anyway.
Great video and thanks. Thats 1/64 tablespoon. A teaspoon is about 1/8 of a tablespoon. So 4x that small one you got there or 1/4 of the big one (the size of a nail) would be a teaspoon.
Why does the spoon say “1/64th TSP” right on it then? It would say TBS if it was 1/64th tablespoon. Either way it doesn’t matter, you can use more nutrients than that. The only reason I used such small amounts was because I had sediment but I found out it was from not using enough water in the pressure cooker. Make sure the water level is touching the bottom 2” of the jars while pressure cooking.
@@SporenSproutthe spoon is wrong :) Americans generally have a different understanding of teaspoons they are really tiny in the rest of the world. I like your content just wanted to point that out
Thanks to these LC videos promoting this bacteriological peptone, Amazon is sold out. I decided to order the soy peptone instead.
Given these results from something sooo diluted, we all need to promote this recipe so there's more peptone to go around as this mycological revolution ramps up 😉.
Good to know at least that LC solutions can be successful over such a broad range of concentrations. I don't think I'll even bother measuring after watching this; just a couple of pinches of each to be sure I have at least this amount, but obviously waaay less than other recipes I've seen.
if I'm using half gallon mason jar should I still PC at 15 psi for 30 mins or go longer than 30 mins? also if using spores syringe, how many cc should I use for the half gallon jar if I need fast colonization?
30 mins is still fine. 1-2 ccs although spores are not guaranteed clean. Best to germinate on agar first and then transfer to liquid. You can do it though, just make sure to test a drop of the final product on agar.
@@SporenSprout doing agar currently is not possible, I got those spore syringes from a very reputable supplier that I had successful spores to grain 10 years ago from their syringes, I'm also following a very strict hygiene including sterilizing neddles in PC and inoculating in well sanitized closed area and using self healing port like the one in video and for the GE I'm using 4× 1/4 inch holes and on each hole I'm using tyvek and on top of it GE filter disk..
@@mo3taz_mizo38 You should be fine but you can easily buy pre made agar plates here if needed: amzn.to/42C7ZrX
How much growth do you get after a week? Is the one you show with that much grow only after 7 days?
So I didn’t use an agar plate, but I haven’t used my spore syringe yet, so I still have time to buy one - how would I transfer it from agar plate to liquid culture ?
It's very easy if you haven't already figured it out. You just cut the area of agar that you want and drop it in the jar.
ive had cultures last 3 years in a dark cool closet..........dont disturb the water wall inside and the mycelium will give off a digestion enzyme that sterilizes the water and essentially puts it in stasis.
After you insert the syringes do you put the jars in the refrigerator?
No, after inoculating the jar it is allowed to grow at 75 F and once it’s full of mycelium you can put in the refrigerator but I prefer to just move them to an air conditioned room instead
I dont have light malt extract but can i use only peptone and syrup?
Yes that would be ok. Or use just 1 TBS of honey.
This week for starting mycelium grow I need to keep it in dark, worm place?
Yes that’s ok. Light is not a big problem
Question: I followed this recipe but I forgot to tighten the lids after I took them out of the pressure cooker. I left the foil on top though. I took the foil off 3.5 hours after removing them from the pressure cooker and noticed the lids were loose so I tightened them at that point. Do you think the jars could be contaminated? Let me know if you think I should start over.
You’ll most likely be fine to use them
You can leave it out in a cool room? Cool like like 65 to 70° ?
70-75 is best but it will just grow slower at colder temps.
can i store it in the fridge or should it just be at room temp. After the mycelium has covered the jar and you want to store it.
After it has grown enough mycelium you can store the jar in the fridge or in a cold room.
@@SporenSprout ty. nice vid btw
Question so Ive seen online that people have said you only need a few drops of a spore syringe to 1ml. So what is the truth can you get away with less ? Or do you need more spores
A spore syringe is not perfectly clean so you should only use 1 ml. If you germinate and clean the spores on agar and make liquid culture from it than you can use 2-5 ml
Do you know the measurements by weight for LME and peptone? Asking because I don’t have measuring spoons.
Just use .5 grams peptone and 1 gram of LME. Mix the ingredients into cold/room temp water not warm/hot water. It this video I used less because I was mixing with warm water and that made it cloudy.
@@SporenSprout dude thank you!
One last question. I received some LC from you and I made a rookie mistake of putting the pink oyster in the fridge with the rest of them. Luckily I got it on to agar before I did that and it’s looking healthy but I’m wondering if I should toss the syringe because I’m out of clean agar plates to check if the LC survived the cold. Think it’s done for?
@@orionsbelt3827 The pink oyster culture will be fine, it is very strong.
@@SporenSprout 🙏🏼🙏🏼
How long/often should LC be put on the magnetic stirrer?
5 minutes every other day is good.
@@SporenSprout I appreciate the info!
Ok mate, I bought some of your liquid culture from you, and have made this mix in this video. How much liquid culture would you recommend me using for each jar of LC?
I use 2-4 ml
@@SporenSprout Thanks, I appreciate the fast response!
Also, what's the chemical symbol on your wrist?
@@RipperTH-camOfficial No problem ! It’s serotonin.
Is boiling tap water sufficient for this method or have you got any advice on how to distil water at home. Having a terrible time getting LC to work with honey currently so definitely going to give this a go!
If you are having trouble making the honey LC media. I recommend making sure the water in the pressure cooker is touching the bottom 2 inches of the LC jars. If you had the water level barely touching the jars or not at all then that was your problem.
@@SporenSproutwill definitely do this. I notice your water is steaming before you mix in the ingredients. Would boiling it be ok prior to sterilising?
@@robinsimpson6654 use hot water but not boiling.
after the mycelium growed enough, can the liquid in the jar replace the liquid cultures on your website? can we inject it directly into a grain to prepare grain spawn?
Yes
@@SporenSprout oh, okay I think I misunderstood. I saw a comment saying you should first germinate the spores on agar. when should this be done?
@@dilaradera You don’t need spores. You can use spores to make mycelium on agar and then transfer the mycelium to liquid culture or just use mycelium from tissue clone to inoculate liquid culture.
Once you got the mycelium grown and stirred you can store it for 12 months untouched or do you still stir it daily?
You can stir it once every 1-2 weeks at that point.
Does this work well for any dung loving mycelium?
Yes
If myc appears flat floating at the top or sunk to the bottom is it dead?
It is normal for the mycelium to grow on the surface and at the bottom.
Can we use it for agar recipe?
Yes I just use LME for agar
Is that filter tape on the lid or is it completely sealed with the exception of the injection port
The lid has 2 holes. 1 hole has RTV injection port and the other hole is covered with 2 layers of micropore tape.
@@SporenSprout awesome! I like it
Is time for growth the same regardless of what mushroom you’re growing? For example I thought Cordyceps were ready after like 4 days tops?
With this recipe the mycelium initially grows faster but will not completely fill the jar like other recipes with more nutrients like honey. Cordyceps can grow for 1-2 weeks
I tried this exact recipe which looked amazing and the only thing I didn't do was use your brand of malt. The corn syrup and the pep tone was the same brand from Amazon. I used Anthony's malt and it will not dissolve. Right now I'm not sure if it's the malt or the peptone but something isn't dissolving no matter what I do with the magnetic stir. They're the same color so it's hard to tell. And guess my only other avenue is eliminating one and then the other to see if they work at dissolving entirely in the liquid
Increase the amount of water in the pressure cooker. The water should be touching the bottom 2 inches of the jars while pressure cooking.
I did a jar without the Anthony's malt and it dissolve fine with the magnetic stir before pressure cooking. I'll try your suggestion on that with the one that still has Anthony's malt in it
is peptone necessary or can i just use malt extract?
Peptone isn’t necessary but it helps boost growth
Great vids, thanks, you would shoot spores into this ?
I’d rather make sure the spores are clean on agar first but yeah.
Just to clarify will this work with spore syringes as well? not just liquid cultures.
Spore syringes are dirty so it’s best to inoculate agar plates first with the spore syringe and then add the colonized plate to LC
thank you@@SporenSprout
Hi everyone this liquid culture technique is a kind of spawn production i want too know in this type of technique would the spawn and mycelium mass production be more or in solid bag like grain spawn ? Which technique would produce more mycelium mass ?
Liquid culture is not spawn, it is a liquid inoculant use to inoculate grain spawn.
3 steps:
Liquid culture / agar plate -> grain spawn -> substrate.
@@SporenSprout if we just consider this technique as mycelium biomass production which techniques will produce more mycelial biomass ? 1kg of liquid culture vs 1 kg of grains ?
dope video. appreciate you sharing this recipe. Question: Can you write the updated recipe here under my comment. I see you have given different answers throughout the comments. Also how much water are you using 400ml or 600ml? Please and thank you good sir! 💪🏽
I used a small amount of each ingredient in this recipe (1/64th teaspoon) because I had problems with sediment forming when using more than a pinch of powder. But I found the sediment problem was from using hot water when mixing the ingredients. If you use room temp or cold water the sediment problem never happens. So you can use 1/4 teaspoon of each powder and 1 TBS of honey/karo. The honey/karo is optional.
@@SporenSprout gotchu. I just made a batch and it has a tan hue to it. Not crystal clear like yours. Do you know what could be causing that? No sediment at all and I can see through it.
@@Mycoky215 I love a golden tan hue so it sounds perfect to me. I just couldn’t get the golden hue without the darn sediment until I started with cold water. The tan hue is usually from having more than 1/64th teaspoon which is ok or it’s from cooking too hot/too long which caramelizes the sugars and can cause the mycelium to be delayed. I know people who use instapots always get that golden color so if you are using that it’s usual.
@@SporenSprout I’m using a 23q presto. So maybe I just cooked it too long. I cooked for 20min at 15 psi. I did let the air vent for 10 min before placing the pressure gage on. So maybe some clarity around your time frame during the PC would help? Thanks again for your quick responses 🤞🏽
@@Mycoky215 make sure you have enough water in the cooker, it can overheat the solution if there isn’t enough. I pre-boil my distilled water on the stove before adding it to the cooker and it helps cut down the time it takes to get to 15 psi. Once it gets to 15 psi (30 mins) I start the timer for 20 mins. Once the timer goes off the cooker is shut off and aloud to depressurize naturally for 10 minutes and then the jars are removed.
😂 I just realized I brought liquid culture from you lol
That’s awesome! 😆 Thanks for the business and for watching! 🍄 Good luck!
Honestly before watching this, I thought to make this you’d use part of the end product, like making a spore print or putting some on to agar. Does this just multiply your initial 10ccs and that’s it or is there a bigger plus that I’m missing?
Spore -> agar -> LC
Yes It propagates the mycelium.
hi bro, can i use the spore syringe ,directly into the liquid culture ? thanks @@SporenSprout
@@ZepHoffman Yes you can but spores are not sterile so there is increased chances of contam. You’d have to definitely test the resulting liquid culture on agar plates. Might as well put a few drops of the spore syringe on to agar plates first and transfer the mycelium to Lc media
@@SporenSprout many thanks bro!
@@SporenSproutwhat's a lc media obviously I'm a noob I wanna do spore syring into culture to but don't want contam
How much LME would you use if the corn syrup is not available?
Just use 1 gram of LME
@@SporenSprout thank you bro, definitely will test
What temperature do you store the jars at while waiting for growth
75 F
Great thank you !
Hi just another question. If using a dark grow tent for mushrooms, do they need any daylight . Or would you recommend 9 hours with a led light of some sort?
@@johnabbott86 Yes I recommend a white LED light strip, 8 hours per day would be good
Appreciate it . Thank you
How long before the jar is ready to go? Before I add them to syringes?
2-3 weeks
@@SporenSprout Do you stir every day or just if you start to see a cake forming at the top?
@@therealderrickwynne Yes I mostly only stir when it starts growing on the surface. After inoculating I like to let them sit for about a week before the first stir, I like how the mycelium looks like a fluffy cloud before stirring. I can pretty easily judge how good the LC media will be based on how it looks at these stages.
600 ml is too much for me for 1 calture. Does the hot water dissolve the ingredients well enough so I can divide the 600 ml liquid into multiplied jars? Thank you.
Yes that’s fine
@@SporenSprout Thank you so much!
how much in weight is it 1/64 spoons of DME and peptone?
Just use a gram of LME and .5 gram peptone and make sure to use presto 23 quart with 3 liters of water in it. I used low ingredients in this video because my all American pressure cooker always ruined my media by making it cloudy and I didn’t realize that for a long time.
Which model pressure cooker are you using? The 921, or 925?
I have the 41 quart, 75x electric all American
@@SporenSprout omg, that has to be a beast! Does that run on 220?
@@RipperTH-camOfficial yeah I love it! 120 volt
Can I use Jam jar for LC?
Yes you could
can i use water from any machine ( water dispenser )
Yes
Do I need a vent along with the self healing port?
Yes I use 2 layers of micropore tape.
is a magnetic stir plate just for convenience or can i just shake the jars
You would have to have a special hydrophobic filter instead of micropore tape. And shaking won’t really break it up that good.
Serious question... why would you need an expensive hot plate to disolve the peptone $ malt extract? Wont that heat up and disolve during the PC?
I didn’t use a hot plate but some people do.
Love this tek, brother.
My cl became cloudy how can in save ? I was using 1g of peptone
Try mixing the ingredients into cold water instead of hot water.