Agar vs. LC (Liquid Culture) Cultivating mushrooms and using different media to grow mycelium
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- เผยแพร่เมื่อ 19 มิ.ย. 2024
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Dude does his video in front of a laminate flow hood to stop the content getting contaminated
Ahah 😂
As a hobbyist, I find it harder and harder to justify my use of LC.
LC is great to quickly multiply your mycelium to inject vast amount of grain, but that's not a situation you encounter often as a hobbyist.
If you are going to do LC properly you need to use agar anyway: you will inoculate your LC with agar, and you will want to test each of your syringe on agar for contaminations.
So I find it easier just to skip the whole thing and just transfer the original plate on additional agar, and chop those plates in halves or quarters to directly drop them in the grain jars.
yes it is for fast multiplication - some species take forever to grow like bluefoot, king stropharia etc. and using Lc saves a TON of time which helps with beginners and impatient folks like myself ha 👍
I've been reaching the same conclusion, I've made 6 LC jars now but haven't used more than two 10ml syringes from each. Soon I'll try agar to grain
So much happier not using LC. Had some terrible results. I can go from agar to grain just fine .
@@Sssanbo this is interesting to me and makes me want to ad more plates to my shop thanks for the feedback everyone!
@@HeyHeyHarmonicaLuke if thats the case you could probably skip it all together and just use grain to grain.. add a few colonized grains to your uncolonized grains. maybe clone the genetics you want on agar once then colonize a jar and from then on out gtg would save an extra week or two. if ur just growing small amounts as a hobbyist none of this is really needed.. i like lc because weather i use it or not i can keep a jar of each of my best genetics in the fridge for years.. i like to have lc for keeping my genetics.. spore syringes/prints for backup. i mostly mess with the lc though cause its faster and you can see if theres contam..
best of luck! mush love to ya!
The only video i found about difference between LC and agar, we really need more videos about difference between methods
by far the best youtube video I've seen in, well, ever. Thank you
Mr. Gary you are great, bravo and good luck.
I really appreciate your style.
I love watching your videos! Thank you so much for sharing your process with everyone. Love how you make the scientific approach accessible for ameteur people like me. Just ordered the book radical mycology because of one of your videos🙂
Awesome thanks for watching! That book is a great reference tool it can be a lot
the first time through just go slow and steady 🙌🍄❤️
Thanks Gary! your lab videos are top shelf! you are a fantastic teacher... MUSHLOVE!
thanks for watching! MUSHLOVE
If a Gary gives advice, listen.
Thank you so much for such detailed information! It is something that is difficult to find on TH-cam.
thanks for watching! ❤️🍄
Thank you...great stuff as always.
I’m moving to Denver Colorado in may and have some good ideas and some break through stuff (yeti) are my project right now
That was her helpful, thank you so much!
very exiciting,,i got i lot of knowledge from your videos,,long live
Exactly what I was looking for, thanks man
Thanks for the explanation.
You have the best looking plates
thanks! I appreciate it!
This is really great info. I'm just moving into the LC and agar medium and moving away from spore syringes. Having good quality information like this is priceless, thanks.
Spores syringes are awesome for finding what you want. They are a shotgun blast of genetics
When I got interested in growing I bought a kit that claimed it had everything I needed and it was fun and easy. And a spore syringe. I didn't use half the things in the kit and didn't even hear about LC until I had failed several times with spore to spawn tech as instructed. LC opened the doors to finally have some decent flushes. There is much misinformation in the mycology arena. There will always be variation in methods etc but it seems like there is also on purpose elimination of essential facts. Certainly there was from the company I bought the kit from. Everything could have been purchased at home depot or the 99cent store for half the price. And those who sell spores don't want you learning about liquid culture because it hurts their business. The whole spore to spawn, especially Uncle Ben tech is in my opinion the worst practice promoted to the public. Serious growers discover that fairly quickly and hopefully learn about LC and agar work. I should have bought the book first. I wouldnt have purchased the kit. But thats the usual way. First the sins , then the bible.
@@jefolson6989 those kits aren't too bad for an individual that isn't interested in learning about mycology and just want the quickest way to grow shrooms so they can trip balls on Friday night. There is a market for that, and those people don't want to take the time to understand what they are doing and to get those supplies themselves.
The problem is when people want to learn about mycology, and they buy these kits, then fail miserably, they don't continue. They give up figuring it's a scam and it's going to be too hard.
I was more interested in learning about mycology than getting high. I took the time to research everything, and get a solid understanding of how to grow, before I attempted my first grow.
I used spores syringes with the PF Tek. The first time I did it I followed the steps exactly, without any adjustments. I did this in order to gain a solid foundation into mycology.
Once I had that understanding I was able to experiment. I changed things and found what worked and what didn't work. I had many more successes than failures. But even with failure I still gained experience.
I don't grow on a commercial scale and I only do it for the fun of the hobby. The fruits are just a bonus, that can help me with my own personal insight. I use psilocybin to better myself and my understanding of my position in this world.
I wish that it wasn't illegal and more people grew mushrooms for the fun of it. I wish the knowledge was easier to get and more people weren't deterred from the hobby. I also wish more people were able to experience the benefits of psilocybin, not just for a good time, but as for a tool for self improvement.
It's so strange to me that it's illegal for me to cultivate mushrooms on my own and invest them as I see fit. This is something that is here in our world for a reason.
Great video, can you do more videos of what cantam looks like in agar and lc?
Thank you so much ❤
Nice to see you upping your video quality :)
it’s getting there! 🍄❤️
@@FreshfromtheFarmFungi don't get too technical on us Gary.
keep the videos as is you were Neil DeGrasse Tyson explaining the center if black holes LOL
@@themyceliumnetwork It just flows out naturally hah I will continue with my best foot forward and consider all levels of knowledge
Very helpful! Thank you!
Pro-tip: Put the fan for your flow hood in another room. It'll keep the sound down and make your lab positive-pressure.
I have another hepa that is just for positive pressure
Dude trying to give a "pro tip" to and obvious pro. Our society is full of idiots
Great video. Thank you.
Personally I don't use liquid culture. I only produce enough mushrooms for me and my family, liquid culture seems to be more for volume producers. And agar doesn't add any more water to my sterilized substrate, I work real hard to get my grains hydrated and dry. Liquid culture adds back too much water. I can add agar pieces from an entire plate and it won't change the hydration level at all. But if I were inoculating 100 bags of substrate I can see the advantage of using liquid culture vs. dozens of plates of agar.
Hey Gary, great content as always! I'm curious, what speed setting do you set your flow good at?
all the way up 👍 just make sure you keep the pre-filter clean
❤️ I love it
At 12:00 thank you for show me how to use that micron filter😹❤️👏🏻 I have lids with lower lock ports and self-healing injection ports. I couldn't figure out how to use that 2.0 filter
Great video
Once again, Thanks for your instruction….very helpful! I’m curious about the size/gauge needle you use to extract the liquid culture from to jar. 🙏
sterile 16/14g works best
he said he prefers 14 on a diff video.
Howdy Gary! I know this is an old video but so you have any videos showing instances of contamination in LC tests on agar other than tric? We've had some issues with LC and a guide for LC testing works be so useful.
Thank you!
hmm this would take a lot of work to explain but I can see what I can do this winter as it would add a lot of value!
Thank you, Gary. Do you also work with an agar/mycelium slurry? If so, I’d love to know what you think about this tek.
I am experimenting with it I will do a video
wow LC can be stored for years? Mine been sitting for a few weeks and I was wondering if they were running out of food. Good to know.
refrigerated at 37-40F most can live a very long time
very helpful
Howdy Gary. Love your videos. You seem like you spent a lot of time learning about mushrooms. I have been watching your videos about breeding mushrooms and I have a question about hybridizing in liquid culture. If it is possible to put quarters from separate cultures together… let’s say two different oyster mushrooms in on dish and then take a culture from where they come together… grow it out put it to spawn… will that produce a hybrid if fruited? I understand that you would have to isolate the spores from the grown fruit and do selections to gain from the hybrid… but would it be a hybrid, and could you do the same thing in liquid culture?
you can successfully mix the same species in a liquid culture with spores - look up the hyperbreeding video with cordyceps 👍
What guage needle do you use on the surringe?
I have had two starter kits for over four months and they both would not sprout. They are both very well covered with mycelium
sometimes it takes patience
Thanks, very informative. Can you make a video on slant culture ?
Slants vs Plates explained: mycology tips and tricks th-cam.com/video/kjAPcc8mEmA/w-d-xo.html I will do a more thorough one soon
So I came across a video last year of someone putting a silicone tube in their LQ when they made the lid. I believe the tube was just attached to a 1 way valve and then was red siliconed to the lid just like the filter is, and the tube went to the bottom of the jar. And then they could attach a 3-way luer lock valve along with a syringe when they're ready to inoculate. Then they just suck up the LQ in the syringe, twist the valve and push it through the 3rd way to the spawn bag. It looked very productive and time saving but unfortunately I can't seem to find it again, just wondering if you've seen the same thing or anything similar.
th-cam.com/video/Fit7U640q1Y/w-d-xo.html here is our lc cap video - also the one from Lions Mane in Denver which has something similar - hope these help! th-cam.com/video/05padpW-M9Q/w-d-xo.html
@@FreshfromtheFarmFungi yassss, thanks so much..!!!
Good stuff Gary, keep the videos coming!
Question, I know you have a good lab set up and practice sterile procedures as much as possible...my question is...what percentage of cultures would you say you still get contamination? Thanks.
my contamination rate is less than 1% these days it just takes diligence in cleaning and patients- it didn’t start that way though and I still am striving for better 👍
@@FreshfromtheFarmFungi Thank-you Gary!
I have noticed with my chestnut mushroom liquid culture it is not totally clear compared to my others. The other jars are crystal clear with blobs of mycelium. Took a fresh piece of agar and grew it out and made another liquid culture and got the same thing. But both produce mushrooms with no contamination. It seems like some of the mycelium do not like to socialize and just stay suspended making it a little less clear.
it could be a polyploid or two cultures but pholiotas can be weird and spontaneously mutate at the leading edge. They are strange indeed
Great information in video thanks. How can I sterelise syringe filters and how many times it can be used for making LC please guide sir
yes you can, the glass ones last a really long time ive been using some of the same ones for 4 years until
they get plugged up
What size needles do you generally use?
Thanks
What is the nutritional composition in Lc?
How do you prepare agar solution?
another highly informative video :)
you mentioned blond morel mycelium.
I have some black morels, is the mycelium the same or will it grow in darker after 2 or so weeks ?
Thanks
It will grow darker and eventually produce sclerotia, this was just the initial growth (24-48 hours)
thanks again for the advice.. I checked out your channel I like it man , that video you did about testing your flow hood/ lab space caught my eye.. I should probably put a few open agar dishes/cups in front of my hood and just run it for a week..see what arises
nice man you got some good info here....
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00:00 Liquid culture and agar are both used to cultivate mushrooms, but liquid culture has a faster growth rate.
01:55 Benefits of liquid and agar cultivation
03:46 Identifying contamination in mushroom cultivation
05:45 Observations on liquid and agar mushroom cultures
07:31 Quality control is crucial in identifying bacteria and mold
09:14 Agar culture allows for two-dimensional visualization of contamination, while liquid culture allows for faster three-dimensional growth with clearer observation of contamination level.
11:14 Using liquid cultures with injection ports and syringe filters is a more sterile technique.
13:02 Liquid culture can last for years but its nutrients will eventually run out
Crafted by Merlin AI.
Hi Gary, I use condiment cups for my agar plates. Instead of using slants could I just fill my condiment cups fuller with agar for long storage in the refrigerator?
yes that would work well too
Yay!
Do you seal your glass Petri dishes after loading with gar to prevent ingress of bacteria?
no, they are not vented and hold a good seal with the weight of the glass - I can do a video on them later
I have a question.
When you are cloning a mushroom, can you just put a piece of it directly to a LC jar instead of an agar plate?
yes I have this video on this here - there are pros and cons th-cam.com/video/3xfufS1C594/w-d-xo.htmlsi=F9dU2iolwcQkBYwC
Great video! quick question do your slant containers have any air exchange like a small hole with micropore tape or is the air inside enough for the mycelium to last till the next time you open it?
the air inside is enough - and maybe there is some small amount of exchange through the threading but it’s negligible
@@FreshfromtheFarmFungi Good to know, damn i have been poking holes and adding micropore tape to a ton of these haha.
@@CMZneu it couldnt hurt but it’s a lot of time and energy 😀
@@FreshfromtheFarmFungi Even with what you said about the years and years of longevity of storage that the extra nutrients are supposed to provide, the internal air is enough that there's no concern?
1st. Thanks for the videos. 2nd I'm routing for you on those Morels. 3rd ?- Do you know a good place to purchase a flow hood?
Doesn't seem like much to build. The parts&wood is definitely over a few hundred
Good luck Morels 🤘🕉️🤘
Thanks! I got mine from Air Science Global in Florida and Myers Mushrooms in Missouri - I have some recommended ones on our amazon affiliate page because the other places have been sold out recently
@@FreshfromtheFarmFungi understandable. Definitely seems like quite an interest in growing mushrooms now...... cough uncle Ben's Tek,🙂.
Thanks, I'll check them out. Doing mostly Shitake & 4 oysters but to be able to clone in front of a "flow hood" seems like a luxury. Best of luck/Merry Christmas
Have you noticed a difference in contamination rates since switching from the Purair FLOW cabinet to the Myers Mushrooms flow hood?
no I mostly do bulk substrates on the myers hood but it is still operating great 👍
@@FreshfromtheFarmFungi good to know! Thank you for the reply! You have an incredible channel. Keep it up man. Hoping to see some morel updates soon!
Hey there! Is this the same hood you did the 5 min open dish test on, and the follow-up video 72hrs later sometime last December? I'm thinking about buying a brand new in box, but 2nd hand from someone locally...Just doing the due diligence, as I've never really seen these until now. Been out of the community for a while :)
We are still using this hood regularly without any issues - you need to clean the prefilter regularly to keep the air flow going strong but other than that it is working great! I would not recommend buying one second hand unless you really know the seller and how it was used. Lots of things can render the filter useless and there is no way to tell without testing - hope that helps! 🍄❤️
@@FreshfromtheFarmFungi Thanks for the reply. That's exactly my reservation. It's from a legit farm, and it was bought as a backup and never used, and then they just sold the farm...but that's not to say that it wasn't moved around a lot, or bumped into and damaged in the meantime....
in slants don't we need air exchange? you showed us a slant with a cap is it ok for it to be air-tight?
there is enough ambient air for slants to be stored
Kuzu göbeği morel nasıl doku kültürüne alabiliriz
Gary if I was to make some spawn plugs with LM and insert into hardwood logs now (Jan 5th) will the next freeze kill the mycelium? I read that it can take up to 12 months for that to work.
It may kill them, I would wait until spring and you should get fruits by fall but why not try a couple anyway and see what happens they have amazed me before with resilience
@@FreshfromtheFarmFungi thanks, good idea I will give it a shot.
Just curious do you have some growth on agar plates that look a little orange or the color of agar? Trying to determine if its contamination. Also peptone in liquid culture=game changer
It usually indicates contamination if it’s colored orange and sweating out exudates 👍 try to transfer from the outer edge and worst case you can culture out with antibiotics
@@FreshfromtheFarmFungi What peptone do you guys reccomend? brands?
Please can you answer this Q, can i use Regular white sugar in this media
Like PDA using Sugar or honey insted of dextrose glucose, and what the ratio would be?
Thank you
I never tried sucrulose but it might work start at 1% and work your way up from there I don’t recommend going more than 4%
Button mushroom lc recipie please
Thank you Gary ..I've been lost inside my lab , staring into my agar ketchup cups.. on the underside of the agar cup I can see colorless, but darker concentrations . Not sure if it's a sign of contamination , However, on the top of the agar it is fully colonized and white as snow. If the top of the agar is all white does that mean that the sample is good?
It’s hard to say without further descriptions and photos - there are contaminants that are white and there can be inert contaminants in the agar media that are sterile but it’s good to observe these things it means that you are paying attention 👍🙂
I may do a video on contamination in the future but it will be a lengthy one ha
@@FreshfromtheFarmFungi Thanks a million for taking the time to respond Gary. I was just telling my brother that out of all the people who im thankful for the most (in regards to my learning) ,and out of all the people I really want to do something special for one day ..it is you. My life depends on this career move working. my stomach is in knots im so nervous about it , but it seems like whatever worry im having or issue im having in my lab..TH-cam senses it and shows me a video where you are addressing that very issue. SO big thanks to TH-cam and Whatever I can do to help you in the future Gary just let me know and im there. peace
@@FreshfromtheFarmFungi and a painful one lol
@@FreshfromtheFarmFungi yes yes please do, I've been battling with what I think is bacteria, every time I make a transfer to clean it I just end up spreading it 😕
When making LC would it be okay to use a Microppose Synthetic Jar Lid Filter Discs with a Adherable Injection Port? Or would the filter size be to much for growing LC since the entire lid would be filter?
This is all I have at the moment.
it might work ok for a run or 2 but it will start to deteriorate over time and is risky long term
A lid with two holes works fine . One hole with rtv as an injection port and the other hole with a piece of filter disk rtv sealed to allow sterile gas flow. I end up using the jar before anything falls apart . Then i just reuse the lid and make a new port and seal a new filter disk.
Hello so when do you test your liquid culture?
I test them after 72 hours of growth because this is the minimum time it would take for contamination to reveal itself if you test on agar. You could do more sophisticated testing earlier as well.
So all the LC needs in it is honey, sugar, and water? All of the search results for LC are all sorts of things in some container to mix with water and it's annoying how vague it is. Thank you for being straightforward.
yes not even sugar just 4% honey water works!
Thank you! I made two LC's, one 4% honey and the other 4% water from the same species and strain of slippery jack, to see what would colonize better when I add it to my grain spawn, will it be the 4% honey?
Is it possible to make LC from spores?
yes there are caveats though, could be higher rates of contamination and also competing phenotypes in the same culture
Bro can we directly introduce liquid culture to the woodchips/rice straw substrates? Please help me
yes it can work but you need to pasteurize the substrate or the nutrients from the liquid media will get consumed by contaminants quickly
Why did your LC contaminated if you used such a Flow Hood?
it can happen from breathing, poor technique, skin particles. It is bound to happen at some point.
Why do people add yeast to pda and what are the beneficial differences between PDA and and MEA?
for nutrition - there are micronutrients in the yeast and different sugars between these two recipes
We're do you purchase your needles and syringes?.
check out our amazon affiliate page www.amazon.com/shop/influencer-a88d1601?ref=inf_own_influencer-a88d1601 they are harder to find these days but wherever has the cheapest ones 😁🍄❤️
Typically.. if your using honey in lc.. you won’t have any contamination.. due to honey being anti bacterial. Hence why honey has been found still good after 100 years or more.
I would like to test the limits of this assumption. What is antimicrobial and is it destroyed during sterilization
@@FreshfromtheFarmFungi this would be a great video. How you would set up the controlled experiment, I dunno.. I use honey, I often wonder if these properties may hinder my grow because it encourages weak or mutated genes perhapes....
How long do you keep you LC on the stir plate each day? 24hr or a few min a day?
I just rotate them on there for a few mins at a time. It would colonize faster to leave it on for longer but I only have one stir plate
@@FreshfromtheFarmFungi Thank you for the reply Gary. You are killing it with these videos. I hope your business is crushing it as well. Thank you!
A gar...or AYYYY gar
I cant with the ogger
@LovieCula seriously, where do they get all these other letters
What is the procedure to degrade plastics by edible mushroom? Pls reply
working on making a video for it
Ijust inherited a collection of slants. How do I get this thick, seemingly impenetrable stuff out of the tubes and into some grain bags? PLEASE HELP? Also, I've been using Ben's 90 Secong Rice bags. Are ppl having luck with these?
Try using a loop, or try adding sterilized water and shaking it up and using a syringe to pull up the mycelium and inject into the grains (this way would render them useless after but you could subculture once the grains get going)
@@FreshfromtheFarmFungi I'm totally new to this. What wd the 'subculture' entail?
I recently QCd my liquid culture to agar and it has been almost two weeks (12 days) and I cannot see any signs of colonization. Poured 2 cc of LC to the agar. Still nothing until today I made the agar a few weeks ago cooled it down, sealed it up with tape, and put it in the fridge after the process. I inoculated the agars using the LC I made out of a store-bought LC. Are refrigerated and sealed agar plates not viable for inoculation anymore? The ingredients I used was 12 grams of mashed potato flakes, 8 grams of agar, 4 grams of corn syrup (Karo), and 400 ml of water.
what type of mycelium and how was the culture stored? It sounds like everything should work out. No growth means the culture is dead or there was no mycelium in the 2 cc distributed to the plate.
@@FreshfromtheFarmFungi thank you so much for the reply. The culture was inoculated around three weeks ago, strong and vigorous growth for the past three weeks. Solution was made using 12 grams dextrose powder, 12 grams karo, and 600 ml of water PCd for 30 mins @15psi. Is it advisable to squirt more than 2 CCs of LC to the agar? Won't it have issues? I noticed that the agar is dry, will it be enough for the LC to rehydrate the agar? I tested one and it is still jelly-like and doesn't have any issues, no contams and all.
I'll try to inoculate the agar plates again soon and let you know once I have a result.
You are the first person I've ever heard pronounce agar like that's lol
It’s my upstate NY accent
Honestly a pint of water and tablesppoon light corn syrup pc and you got lc
Do you have a site where we can send you pictures
you can send email to freshfromthefarmfungi@gmail.com
Is there any flow hoods for small growers?
That are under $1000 :/ they are all so expensive just for a shoebox grow
yes try the vendor “nothing but air” on instagram he refurbishes flow hoods and has good deals and sometimes gets smaller ones - also hood ratz I believe makes 2’ x 2’ ones or look on etsy - these are usually hand made and sometimes do not work as well as professional engineered ones
sir for oyster mushroom which liquid culture recipe is best for high yeild?
I haven’t tried them all but honey, karo, tsb, bpw all work well
@@FreshfromtheFarmFungi thank you so much sir
What's the sterilization process for your honey water
20 min at 15psi/250F
My honey always caramelized and cloudy till the mycelieum takes over.
Why don't you use a rack to. Lift up your stuff ?
not necessary
Where can I buy this kind of jars?
amzn.to/3OkcXBZ these are the jars these are the lids www.etsy.com/listing/990993932
@@FreshfromtheFarmFungi awesome! thank you!
Question: Couldn't you just keep adding sterile liquid culture media to your LC bottle to keep it going longer?
You could just use the old LC to inoculate a new one and essentially do the same thing
yes if you do it correctly and don’t introduce contams
I am a little confused about the honey. I heard that already a few times but still have questions. In honey one of the only spores of anything that can survive in it are from the bacterium Clostridium botulinum, which can cause botulism. That can get fatal really fast. I'm sure you can get rid of the spores and bacteria, but improper sterilisation puts people at risk, doesn't it? If it's not, I would love to hear from somebody with much more knowlage than me why that's the case. And if it could be a problem maybe a disclaimer would be nice to have.
clostridium is ubiquitous in nature and present almost everywhere in the soil and is why it’s so important to sterilize your culture media just like canning vegetables. This honey solution is diluted and sterilized and clearly supports the growth of the fungi being cultivated along with many other organisms like yeast (mead is made from honey). Also, this solution is just a media for the mushrooms to grow to transfer onto grains or other substrates. Quality control the media on agar to ensure sterility - all of this info is covered in my channel. There are a lot of people doing lc’s in the industry and I am very careful about ensuring quality control and try to make that clear.
@@FreshfromtheFarmFungi Thanks for the fast and detailed answer. I’m new to the channel but am eager to learn and you are doing a great job.
Sterilization?
Very interesting video as usual.
If you are looking for ideas for a future video, I would really like a video on genetic because there is a lot of 'bro' science out there and would appreciate your more professional opinion on stuff like:
- if you keep 'sectoring' on agar you will have a monoculture. Is it true ? Is there any point in having a monoculture ?
- if I clone from a fruit, do I have a monoculture or are fruit the expression of different genotype cooperating together ?
- I see people restarting from spore after a while to avoid senescence after several generations of cloning. But usually they do that from a single spore print from a single fruit, so don't you have kind of inbreeding ? And you are just perpetuating the same 'washed out' genotype ?
Thanks,
thanks for these ideas I will try to tackle them to the best of my knowledge 👍
Here is my opinion if it helps...
1_ Yes, that's the point of sectoring, yes because all the mycelium will be one strain and if it's a good strain you will get good incubation times and good flashes instead of leaving it to chance. It's the same reason people clone/graft hass avocados trees, you know what you are getting.
2_ If you clone correctly yes, as far as i know mushrooms only contain tissue of one strain and if it is mated(can produce fruit) the mycelium will be dikaryotic meaning it contain two nucleus one for each of the mated pair.
3_ While senescence is a thing in ascomycota(cordyceps, morels, etc) i haven't found any reliable info that this happens to basidiomycota(most gourmet mushrooms like oysters) in ideal conditions. Inbreeding isn't automatically bad, especially in mushrooms and as long as you select for good traits you can get good strains while inbreeding.
Can you reuse a syringe by sterilizing it with alcohol?
it wouldn’t be completely sterile - some endospores could survive but it might work well. I used to soak the rubber stopper in disinfectant overnight and sterilize the plastic in a pouch in an autoclave that works really well but now I use glass syringes whenever I can and those can be autoclaved hundreds of times 👍
I fill with alcohol, and also leave the needle submerged in alchol... I hope it's killing those spores cause I just did some LC transfers and spore to agar... I'm new, so I can't vouch for effectiveness.
I want a recipe for liquid medium to culture Mycorrhiza if you don't mind thanks :)
it depends on the species this would be a complex matrix because of the nutrient requirements that the roots provide - we used honey but it only works temporarily
@@FreshfromtheFarmFungi mycos brand amazon prime sells it
@@edchoi2428 Link please, need info whats in there
How long can I keep liquid culture in room temperature?
as long as it remains sterile some can remain viable for months or years they will form a layer at the surface like a scobi
@@FreshfromtheFarmFungithanks. I discovered your channel few days ago. Very nice and valuable content Gary!
ah-gar
I cant get rid of l.subtilis ! So bad
How can the mycelium breath in liquid culture shouldn’t it be able to breathe oxygen in ?!
there is a .22 micron syringe filter that is built into the lid for gas exchange - you need to plate and subculture from the leading edge until the contamination is resolved and no longer present (the mycelium should outrun the contamination)
@@FreshfromtheFarmFungi no the bacillus is in my wheat grains im trying with my pressure cooker and it seems that the bacillus is kinda hard. What my question was is that how a fungi mycelium is capable of breathing in a liquid medium ?
I want to solve something right now it is a agar with a hard A I've worked in Labs all over the country as a matter of fact even some in the UK and every PhD that I've ever met called it agar with a heart A
I always start on agar whether using a print or syringe.
From there I use the "Josex Poke" method to get a clean sample into my LC.
Hasn't failed me yet.
+1 for team LC.
LC just neve works out right for me for some reason. I don't know if my mycelium strands are too big and I don't get many noc points after shaking or something. But yeah the poke is a good way to get it going at least. Though I think using a PF puck and scraping that to bits and putting that to a grain master is my favorite way now.
I believed that , logically , one should be able to innoculate LC directly to substrate but I dont think its ever been done. LC , IMO, makes the grain too wet, and causes mold and rot before full colonization. Forget Uncle Bens! The rice is already over cooked for our purposes. Adding more liquid is a disaster. So I went back to grain jars, innocd with small ammount of LC. It works but not tremendously fast.
I have gone straight to bulk from Lc it works but isn’t ideal for my space because I quickly fill my incubation room with mostly uncolonized substrate. I will be doing a video soon to try to mitigate this issue. But yes, under-saturating in preparation for lc is going to give better results
I have innoculated hundreds of lbs of substrate with liquid culture.
@FLORIDAMANIAM-he2lz cool. Your own substrate right? The uncle Ben method never worked for me . And some reccomend spore to spawn directly into Uncle Ben bags. Just too wet to start with.
Sir great content but please sound it out A gar. Your saying auger
ah” lol this is how I was taught in university and the micro labs I worked in for years. “Ay-gar” is a midwest thing that has carried a lot of momentum on youtube but these two pronunciations are in fact the same substance.
can you please share your agar recipe please!!!
it’s on our amazon affiliate page we use the hardy diagnostic pre-blends
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Weird how far people go to control contamination with heat and alcohol.
I grow shrooms without boiling and I dont get contamination..
at larger scales it definitely makes a difference