Liquid Mushroom Culture from Gourmet Oyster Mushrooms, New Episode in How to grow Mushrooms Series
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- เผยแพร่เมื่อ 26 มิ.ย. 2024
- All the details you need to know on how to make a liquid mushroom culture of a gourmet oyster mushroom, although this technique can be used for 100's of types of mushrooms.
Liquid culture consists of a sterilized nutritious solution that has been inoculated with fungus spores or mycelium.
Andrew goes over each step from sanitization and to shows you exactly how to take a sample directly from the mushroom, put it into the liquid solution and how to care for the culture as it grows.
You can find all of our liquid cultures available here,
www.mossycreekmushrooms.com/l...
If you are seeking Mossy Creek Mushrooms, they are here!
King Blue Oyster
www.mossycreekmushrooms.com/p...
The Mossy Creek Mushrooms Pathfinder Liquid Culture
www.mossycreekmushrooms.com/p...
Golden Enoki
www.mossycreekmushrooms.com/p...
Blue Oyster Liquid Cultures
www.mossycreekmushrooms.com/p...
Chestnut Liquid Cultures
www.mossycreekmushrooms.com/p...
Golden Oyster
www.mossycreekmushrooms.com/p...
King Oyster
www.mossycreekmushrooms.com/p...
Comb Tooth
www.mossycreekmushrooms.com/p...
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#liquid #culture #mushrooms #keepspawningculture
![[How to Grow Mushrooms] Making a Grain Spawn Master using liquid culture and agar.](http://i.ytimg.com/vi/njEi_BPBgHs/mqdefault.jpg)
![[How to Grow Mushrooms] Making a Grain Spawn Master using liquid culture and agar.](/img/tr.png)
The needle biopsy move is a good idea. Never thought of using the needle tip for a quick and easy way to get a piece of tissue
I came here to say this too! :)
Thanks for the video MCM. We use needle biopsies for most tissues. The mushroom is dry compared to human tissue but anything that is soft can be biopsied pretty easy.
Wow, mind is blown! Can’t wait to try this out! Thank you so much from the bottom of my heart lol
Those are the biggest oysters I've ever seen. Well done!
Thanks for the comment about stirring releasing Co2 and allowing O2 in. The fish tank analogy helped. I have some liquid culture growing and I was stirring twice a day, I just put it on the stirrer and turned it on, and will leave it there.
I finally feel like i know enough to be able to do the full circle from start to finish to restart again..
Thanks, subbed and will check out some more videos.
thanks so much for sharing this beautiful knowledge about mushroom. I've always desired to start cultivation of Oysters. this is a great eye opener 👍👍👍
You're the man!!! I had no idea I could do a tissue biopsy
WOW...I LOVE THE STOREROOM...AMAZING
In labs liquid media is the preferred method for culturing, simple Erlenmeyer flasks are stoppered with Cotton wool plugs and then wrapped in tinfoil to make sure the wool does not get wet in the autoclave. Color changing Autoclave tape is stuck to the bottle to indicate the bottles that got sterilized. You culture a generation one or more master mediums and then fill the flasks from these. You can also inoculate from a petri dish, mushroom or another liquid culture by first sterilizing an inoculation loop in a flame and then transferring your subject (save on syringes). For agitation we use a lab shaker vs introducing a stirrer mainly as you can shake multiple flasks continuously while incubating them. Consider a flask and cotton wool to save $ and an inoculating loop over syringes, unless your selling them. Also consider experimenting with say a 2-4% Glucose solution for media. If you really want to get crazy look at methods like pulsed electric field sterilization of bulk substratre or growing mushrooms continually in liquid on raft scaffolds like hydroponics but for mushrooms - fungiponics :D Looking forward to your next video.
True. For the Stock Culture we use a slant agar- slower growth, lasts longer.
Then transfer to petri dish agar as Mother Culture (using inoculating loops).
Then from petri dish to liquid broth in hundreds of flasks on shakers (using inoculating pipes/borer-don't remember the exact term). Lesser liquid in larger volume flasks results in much higher growth speed but means more flasks and shakers needed.
For really large scale we used Fermenters instead of shakers.
I think we never used a single syringe for production. We use glass or metals apparatuses that can be autoclaved and reused indefinitely. Single use stuffs are for the hobbyists not professional production.
Just sharing..
Great insights! Thanks for sharing them! I'd love to explore 'fungiponics'! Would you have any links with more information about this method?
Do you have links to fungiponics ?
So you are saying, I can have unlimited liquid culture by simply using ready liquid culture to expand into diff jars?
@@EarsTwoHear yep totally, just gotta make sure to keep everything sterile
impeccable timing my good sir!
I've seen so many videos and nobody has shown this technique yet! Love it.
Thanks man. I used this technique for the first time with a still air box. 1 mushroom to 3 different jars. All 3 jars cleanly colonized :)
Love you Andrew!! This vid been on my to watch list for a couple days. Great one
Thanks!
Great video your content is consistently top notch. Please keep up the good work. And thanks for sharing.
Thats really cool I always go scalpel to agar to lc but I really like this for cloning. Gonna go for it! Thanks
Awesome technique. Beautiful. Can't wait to try it. I'm still relatively new to growing but recently I just used clean tweezers to pull some mycelium from a block and put it in lc. Totally worked. I'm stoked.
Wow. I have always gone fruit to agar to second agar to liquid culture. Good to see this is possible. I will try direct to LC. I have some LC jars cooling right now. It is my first time using the grey leak proof lids. So far, so good.
This is absolutely fascinating! I’m just getting started with mushroom growing, and the more I learn, the more interesting this becomes.
@Sarah Delaney no, that’s not sketchy at all 🙄
Excellent technique! I'm considering doing this with morels. Im going to try to go from a jar to a 5gal bucket. Then dump the bucket over a prepped burn site in the fall.
Making liquid culture tonight--inoculating it tomorrow! Thanks Mossy Creek Mushrooms. 😊Jen
Awesome video. Thanks for sharing.
Thanks for informative video! I will be trying this.
Thanks for sharing; this is a very helpful lecture
I love your channel i learn so much, i always thought LC was stirred to disperse the mycelium, sorta like how shaking grains at partial colonization to spread the mycelium better.
Awesome! thank you for sharing.
Thank you so much! I learned a lot of useful things for myself!
Good methods promote growth, great video!
Thank you for putting this out there it really helps everyone
Hello Mossy Creek mushrooms. I went to a location yesterday that was supposed to be monster mushroom company had to drive for a little while to get there I got there and the people were rude to me they did not have the thing that I wanted they did have some sterilized grain and some sterilized substrate so I bought a bag of sterilized substrate for $30 got home with it and come to find out there is a hole in it and where they tried to seal it there is a hole big enough for me to stick four fingers and and it goes straight down to the substrate so I did not get a bag of substrate that was sterilized I got a bag that was unsterilized really frustrated at this point and probably am going to be looking at what you guys have for sale.
:( Man, that sounds like a bad day. I hope your able to get what you need and well, it doesn't seem hard to beat the competition uh?
He's really an isopropyl addict. Great video of a stellar technique.
Aseptic processing OCD, it sucks to have loss from contamination, so you got to be diligent.
So cool. Thanks for your guide :)
Thanks dude, I totally learned a new technique from you today! Thanks for sharing. I also purchased some liquid culture pre-mix (and used your code) from shroomsupply. I will give LC another go because my first try using Karo corn syrup was great at growing mycelium and mold no matter how clean my process was using a SAB. I also noticed when using your flow hood you should elevate your work surface just a bit so its not right at the very bottom of the flow hood. Anyway great vid, can't wait to try this out.
Best vid on the net!
Thanks man! great video
A wonderfull technic ... i ve learned from you about 1 year ago.. and i ve now many jars like yours and selling them .. Thank you my friend ✌
Fantastic videos! Thanks so much for all the effort and time sharing this valuable information.
What do you inoculate your Masters Mix bags with? Grain? or directly from the liquid culture? Liquid to grain to Masters Mix?
Thanks so much and please keep up the great videos!
i will definitely try this, Thank you
Good teaching, thank you
I use a technique to clone mushrooms directly in the forest. I take some sterilized butyl cap vials filled with liquid medium and use only a needle with a cilinder inside, without the syringue, to push out the piece of mushroom tissue. From now I'll use this technique, as only needs the liquid in the vial to push out the tissue.
Thank you for the info.
I love this idea..
Great videos, very didactic. Thanks a lot.
Michel from Belgium, in Europe
Tunes.... Love it
Thanks!
Cute puppies! Great info! Keep rocking!
Thank you! Will do!
Great video Bro, thanks for the info
Nice Breakdown
Dude , you are the only one I see that teaches how to cultivate mushroom. Thank you
GOURMET mushrooms ;)
One of the best! Thanks mate
Glad you like it!
This is amazing, thank you!
Awesome, engaging video
Wow this is amazing
Just saying hi from Mumbai , kindly keep those videos coming
First time I've heard someone say alchyhol other than me. Big love
Hahaha That's great!
Awesome technique. I need that book.
thanks for sharing
its all in the Twist '''''!!!!
Thanks for the video brother!
NP! Thanks for stopping by!
I appreciate sharing your secrets.
Glad you like them!
Great video.
Thank you!
Awesome video!
thx dude! greetings form poland!
Great video
You guys are awesome. I'm just getting into mycology and want to work for a mushroom company until I can start my own company. Valuable info.
Great! I get more exp watching this. Thanks big man👍
Glad to hear it!
Thank you. Thiis is what I have been looking for.
I get my from an online store, they got shroom, psychedelic and microdosing products well refined, also ships to anywhere.
He's on Instagram with the below handle..
Mycopete.
yiur vidoes are so clean and leaborate
Great video mayne 🤗
It was an epic match to the end ☠️
thanks a lot !!
good explanation bro
Very impressive!!! Well done!
I learn so much from YT videos. I now have another channel to add.
Love that room you have! I wish
muchas gracias por compartir tu conocimiento :D saludos desde Chile! (L)
Awesome!
Thanks!
That's so cool. Not messing around with spores and just using fresh shrooms. I've read that 0.3% hydrogen peroxide kills contaminations and spores but allows to grow the mycelium. However I have found no videos yet.
Thank you sir
Big fan of your videos sir 👌👌👌👌👌 all my liquid culture is totally successful only because of u sir…
Very cool.
Thank you!
Thank you Mossy Creek for all your videos. There are many channels out there but I love your videos. As a hobbyist I find you sooooo helpfull. I've been trying Lion's Mane without success. With liauid culture I hope it works this time. Any tips wouls be welcome. Happy myco!
Great video 📹 👍
Glad you enjoyed it
nobody is talking about how cute hes and he got a lovely smile
Thankyou❤
Hey man thanks for the useful info! Do you only do one needle biopsy per jar of LC?
Lashings of isopropyl - love it 🤣
Hi Andrew. First, thank you for your YT channel. I'm new to home growing of mushrooms and have found your videos very helpful and full of great information! Secondly, I will be using your discount over at Shroom Supply, so thank you for that as well! My question regarding liquid culture is this: how long should I place my culture jars on the magnetic stir plate each day? I'm starting working with only two types of gourmet mushrooms (oyster and lion's mane), and I've already ordered culture syringes for both types, but once the rest of my supplies arrive and I inoculate the prepped/new culture jars, what length of time will I need to let each jar sit on the stir plate? Thank you in advance for any advice/guidance. And Happy Thanksgiving to you and the entire Mossy Creek team! Peace & Respect.
thank you for the video. love your Mjolnir brother ;)
Great video- I wish I could subscribe twice.
This man's iso game is strong!!
Spinning mycelium - "Jörmungandr twists and turns, mighty in his wrath..."
here's a few suggestions to try
pick the best type - there are many available.
grow them in the best environment - some eg oyster muchrooms like cool humid conditions and may be placed outside (I read these and the reasons they work from Gregs Mushroom Grower site )
The eyes are full of primal hate
O
Healthcare best practice for stetile technique is to wipe in a reverse spiral ... that is to start in the middle and spiral outward. I think it would be best to end by coming off of the table away from the fan.
Might be overkill but its evidence based.
I came up with the same process on my own, just searched to see if anyone else was using it--pretty cool. mine method is slightly different but essentially the same.
If someone is really OCD about sterilization they can put items under UV-C lamps as well as pressure sterilization ... I use them for my breathing masks and filters ...
I don’t think you’re using too much alcohol let’s face it it’s better to be safe than sorry I’m just glad you’re not running sterile needles through a flame to make sure they’re sterile like so many other people do
How long should you stir for? Also once the culture is established, how long will it last on the shelf? How do you store it after the culture is established?
I haven't seen that much isopropyl since 2019... how did you clean everything this year?
hahah for real, i wanted to clean my computer back in july or so, and had to get alcohol swabs because the entire alcohol shelf was barren at every store... even the witch hazel was always gone!!
luckily it's back on the shelves now and i grabbed a full gallon, since there was a 1-per-shopper limit xD
I still used alcohol. We bought from the local distilleries that converted over to making hand sanitizer.
Genius! Thanks for the idea. I guess there is no need for agar plates now :D
First I need that beard friend! This is cool I love earth
Really apreciate your content guys...so usefull!! Just one doubt...does the spraying bottle has 70 grade alcohol inside?? Is it the same you have in the other container you use for wiping down the table?
Hey great video so if I have a culture already created how much would you use to cultivate a new jar that hasn't been inoculated?