Thanks for the comment about stirring releasing Co2 and allowing O2 in. The fish tank analogy helped. I have some liquid culture growing and I was stirring twice a day, I just put it on the stirrer and turned it on, and will leave it there.
Thanks for the video MCM. We use needle biopsies for most tissues. The mushroom is dry compared to human tissue but anything that is soft can be biopsied pretty easy.
Excellent technique! I'm considering doing this with morels. Im going to try to go from a jar to a 5gal bucket. Then dump the bucket over a prepped burn site in the fall.
I use a technique to clone mushrooms directly in the forest. I take some sterilized butyl cap vials filled with liquid medium and use only a needle with a cilinder inside, without the syringue, to push out the piece of mushroom tissue. From now I'll use this technique, as only needs the liquid in the vial to push out the tissue.
Thank you Mossy Creek for all your videos. There are many channels out there but I love your videos. As a hobbyist I find you sooooo helpfull. I've been trying Lion's Mane without success. With liauid culture I hope it works this time. Any tips wouls be welcome. Happy myco!
I finally feel like i know enough to be able to do the full circle from start to finish to restart again.. Thanks, subbed and will check out some more videos.
thanks so much for sharing this beautiful knowledge about mushroom. I've always desired to start cultivation of Oysters. this is a great eye opener 👍👍👍
hahah for real, i wanted to clean my computer back in july or so, and had to get alcohol swabs because the entire alcohol shelf was barren at every store... even the witch hazel was always gone!!
Healthcare best practice for stetile technique is to wipe in a reverse spiral ... that is to start in the middle and spiral outward. I think it would be best to end by coming off of the table away from the fan. Might be overkill but its evidence based.
That's so cool. Not messing around with spores and just using fresh shrooms. I've read that 0.3% hydrogen peroxide kills contaminations and spores but allows to grow the mycelium. However I have found no videos yet.
Psychedelic’s definitely have potential to deal with mental health symptoms like anxiety and depression, I would like to try them again but it’s just so hard to source here
Psychedelics are the reason why i didn’t take my life when i was at my end. I was stripped of my ego and saw the beauty of life and interconnectivity and even though i still battle anxiety and depression, I’m doing better everyday and will never think in such a self destructive way again.
LSD and mushrooms completely changed my whole outlook on life. I became a better version of myself This experience gave me a lot of confidence about my self and my body. A bunch of bad thought / behavior patterns were broken. One of these was pretty bad OCD that made me wash my hands a lot. It gave me a lot of hope that things will be fine, this is the one thing that I heard throughout the trip: Everything is alright. The main reason for the trip was my severe depression and it definitely helped me (although it's not gone). Before all I could do was lay in bed. Now I am trying to rebuild my life one step at a time which wasn't possible before."
Hello Mossy Creek mushrooms. I went to a location yesterday that was supposed to be monster mushroom company had to drive for a little while to get there I got there and the people were rude to me they did not have the thing that I wanted they did have some sterilized grain and some sterilized substrate so I bought a bag of sterilized substrate for $30 got home with it and come to find out there is a hole in it and where they tried to seal it there is a hole big enough for me to stick four fingers and and it goes straight down to the substrate so I did not get a bag of substrate that was sterilized I got a bag that was unsterilized really frustrated at this point and probably am going to be looking at what you guys have for sale.
Great content - I like the idea of the needle biopsies better than going from tissue to agar and then liquid culture. Less opportunity for contamination by exposure. Question: What is the best needle gage to collect a proper sample? Also, at what temperature do you store your liquid cultures while they propagate? I am using 1/64th teaspoon of peptone, 1/64th teaspoon of light malt extract, and 1/2 tablespoon of corn syrup with 600 ml of hot distilled water. What is your favorite recipe?
1) Put gloves on, spray gloves with alcohol 2) spray table, spray jars, spray the floor and the air for funsies until I flee slightly light headed from all the iso in the air 3)Spray my spray bottle with separate iso sprayer 4) Spray my hands again, spray the jars again 5) Spray the table one more time for good measure 6) Innoculate a jar or two 7) Spray the shit out of the table, and my hands again 8)Flood lab with 500 gallons liquid death 9) Drain lab 9)Continue work as per usual Few questions How often do LC jars need to sit on the magnetic stir plate? How long can they sit without being stirred? Looks like the grey lids you're using are the new "leak proof" lids for mason jars. Just curious what are your thoughts on plastic mason lids vs metal? Rust aint fun to deal with but even Roger Rabit on the forums at the shroomery holds the opinion that plastic lids contaminate more. Seems unlikely you would be using them so much if that was your experience. Thoughts?
Once a day is good enough for stirring with magnetic stir bar. More would be better. As for lids plastic works just fine. Just tighten again after autoclave as they sometime get loose. The whole contam thing has prolly more to do with the filter disc getting too wet and bacteria getting in that way.
I love your channel i learn so much, i always thought LC was stirred to disperse the mycelium, sorta like how shaking grains at partial colonization to spread the mycelium better.
How long should you stir for? Also once the culture is established, how long will it last on the shelf? How do you store it after the culture is established?
If someone is really OCD about sterilization they can put items under UV-C lamps as well as pressure sterilization ... I use them for my breathing masks and filters ...
Wow. I have always gone fruit to agar to second agar to liquid culture. Good to see this is possible. I will try direct to LC. I have some LC jars cooling right now. It is my first time using the grey leak proof lids. So far, so good.
How long do you recommend each stirring session (if done daily) should be, if using a magnetic stir plate? 5min.? 15min.? Btw, this is definitely my favorite cultivation channel as a total newbie. You're extremely thorough and give fantastic explanations, actually describing *why* things are done, rather than just saying "do this" because it's what others say to do. Plus, I love Norse culture too (I see the Mjölnir necklace and the Icelandic magical stave on the door) so that's a bonus🤘🏻 Anyway, thanks for all your videos!!!
I don’t think you’re using too much alcohol let’s face it it’s better to be safe than sorry I’m just glad you’re not running sterile needles through a flame to make sure they’re sterile like so many other people do
Thanks dude, I totally learned a new technique from you today! Thanks for sharing. I also purchased some liquid culture pre-mix (and used your code) from shroomsupply. I will give LC another go because my first try using Karo corn syrup was great at growing mycelium and mold no matter how clean my process was using a SAB. I also noticed when using your flow hood you should elevate your work surface just a bit so its not right at the very bottom of the flow hood. Anyway great vid, can't wait to try this out.
Awesome technique. Beautiful. Can't wait to try it. I'm still relatively new to growing but recently I just used clean tweezers to pull some mycelium from a block and put it in lc. Totally worked. I'm stoked.
here's a few suggestions to try pick the best type - there are many available. grow them in the best environment - some eg oyster muchrooms like cool humid conditions and may be placed outside (I read these and the reasons they work from Gregs Mushroom Grower site )
This looks a lot easier than anything else I've seen! Thanks for sharing! I have been told that DNA degeneration happens when you take tissue clones from clones. Have you found that to be a problem?
As a microbiologist/geneticist I can tell you that this is NOT a problem. You are not making a copy of a copy. The analogy that would be more fitting is you are taking a cutting from a tree that was grown from a cutting.
@@michaelgrouse7850 oh that makes more sense. So it's more like propagation rather then a clone thing. Ya when i think of clones i think of the star gate series with the Magvyer guy. The one alien species was like dying off cause they couldn't reproduce so they cloned themselves and was like dying off. Haha it's good there's real geneticists out there to counter hollywood and the vividness dilemma.
My liquid culture solution is easy peezy. Gummy Bear TEC. I use 3% gummy bear by weight = roughly 1 bear per each 100 ml water. Organic Black Forest Bears are my preferred brand. There is a little gelatin in there as a protein source. The lower ph from acid and juice might reduce contamination?
Can you give me more info? Someone gave me a bunch of those gummies, and I want to use them before they turn funky, and that looks to be a nice, practical use. Do I just dissolve the little boogers in my water?
@@melhawk6284 Start with a ball jar with a liquid culture lid (and a stir bar if you have a magnetic stirrer). Add 3 grams gummy per every 100 grams water. Pressure cook the jar for 30 min at 15 psi. Cool. Inoculate. Stir the jar regularly while culture grows.
I shot you an email about your thoughts on liquid culture jars and making your own. Did you see it? Additionally would be great if you could make a video explaining generation and how they apply to liquid culture. Maybe how you track them and how important is it to track them. Just a bit confused on that topic I guess. Keep up the GREAT work and THANK YOU!
Fantastic videos! Thanks so much for all the effort and time sharing this valuable information. What do you inoculate your Masters Mix bags with? Grain? or directly from the liquid culture? Liquid to grain to Masters Mix? Thanks so much and please keep up the great videos!
I came up with the same process on my own, just searched to see if anyone else was using it--pretty cool. mine method is slightly different but essentially the same.
Hey bud.. I just wanted to say a couple of things. First.. yalls lab is BAD ASS! I am quite quite envious. I intend to be building one soon as well. Wont be as big as yours but.. if all goes right and the Good Lord Jesus wills it so. its gonna turn some heads :) If and when I build it, I may make a video to show you. And lastly.. I dont think you use too much alcohol at all actually. I think it is perfect. I plan to do it exaclyyy how you do it with the alcohol. Better to have a bunch of nothing go wrong, rather than use a little and all hell breaks lose. Keep up the good work bud! :)
@@vinodpunj3102 I'm not expert but I would probably say no. If it's completely dry it probably doesn't have any actual living tissue to multiply itself from.
If you guys are still wondering, it does work. I ran the idea to a friend who has a certain interest in mycology. He gave me a few dehydrated (probably with cool air) samples that he had sitting around and forgot about for over a year and a half. I didn't have a needle at the time, but I used an exacto knife that was wiped down with alcohol and flame sterilized to carve a small chunk out, after exposing the center with the same process splitting it open like in this video. The other thing I did different was adding it to an agar recipe I learned instead of to liquid culture. Did a few more times in case of any possible contamination from the first, which I did have a couple contams, but with a lot more that were successful. Not sure if mushrooms dehydrated with heat would have any possibilities as well, or mycorrhizal varieties that usually need a plant host to survive. Will probably run tests on those theories eventually, but for now I am just excited with the success I had with this. And I hope it helps others out too.
![[How to Grow Mushrooms] Making a Grain Spawn Master using liquid culture and agar.](http://i.ytimg.com/vi/njEi_BPBgHs/mqdefault.jpg)
![[LIVE] : ONE ลุมพินี 80 | คู่เอก "รักษ์ vs ยอดนำชัย"](http://i.ytimg.com/vi/ckLMRorsMeY/mqdefault.jpg)
The needle biopsy move is a good idea. Never thought of using the needle tip for a quick and easy way to get a piece of tissue
I came here to say this too! :)
Thanks for the comment about stirring releasing Co2 and allowing O2 in. The fish tank analogy helped. I have some liquid culture growing and I was stirring twice a day, I just put it on the stirrer and turned it on, and will leave it there.
Thanks man. I used this technique for the first time with a still air box. 1 mushroom to 3 different jars. All 3 jars cleanly colonized :)
Those are the biggest oysters I've ever seen. Well done!
Thanks for the video MCM. We use needle biopsies for most tissues. The mushroom is dry compared to human tissue but anything that is soft can be biopsied pretty easy.
He's really an isopropyl addict. Great video of a stellar technique.
Aseptic processing OCD, it sucks to have loss from contamination, so you got to be diligent.
Excellent technique! I'm considering doing this with morels. Im going to try to go from a jar to a 5gal bucket. Then dump the bucket over a prepped burn site in the fall.
You're the man!!! I had no idea I could do a tissue biopsy
I use a technique to clone mushrooms directly in the forest. I take some sterilized butyl cap vials filled with liquid medium and use only a needle with a cilinder inside, without the syringue, to push out the piece of mushroom tissue. From now I'll use this technique, as only needs the liquid in the vial to push out the tissue.
Thank you Mossy Creek for all your videos. There are many channels out there but I love your videos. As a hobbyist I find you sooooo helpfull. I've been trying Lion's Mane without success. With liauid culture I hope it works this time. Any tips wouls be welcome. Happy myco!
First time I've heard someone say alchyhol other than me. Big love
Hahaha That's great!
I finally feel like i know enough to be able to do the full circle from start to finish to restart again..
Thanks, subbed and will check out some more videos.
I've seen so many videos and nobody has shown this technique yet! Love it.
This is absolutely fascinating! I’m just getting started with mushroom growing, and the more I learn, the more interesting this becomes.
@Sarah Delaney no, that’s not sketchy at all 🙄
Dude , you are the only one I see that teaches how to cultivate mushroom. Thank you
GOURMET mushrooms ;)
thanks so much for sharing this beautiful knowledge about mushroom. I've always desired to start cultivation of Oysters. this is a great eye opener 👍👍👍
Cute puppies! Great info! Keep rocking!
Thank you! Will do!
I haven't seen that much isopropyl since 2019... how did you clean everything this year?
hahah for real, i wanted to clean my computer back in july or so, and had to get alcohol swabs because the entire alcohol shelf was barren at every store... even the witch hazel was always gone!!
luckily it's back on the shelves now and i grabbed a full gallon, since there was a 1-per-shopper limit xD
I still used alcohol. We bought from the local distilleries that converted over to making hand sanitizer.
Healthcare best practice for stetile technique is to wipe in a reverse spiral ... that is to start in the middle and spiral outward. I think it would be best to end by coming off of the table away from the fan.
Might be overkill but its evidence based.
yiur vidoes are so clean and leaborate
Just saying hi from Mumbai , kindly keep those videos coming
That's so cool. Not messing around with spores and just using fresh shrooms. I've read that 0.3% hydrogen peroxide kills contaminations and spores but allows to grow the mycelium. However I have found no videos yet.
Psychedelic’s definitely have potential to deal with mental health symptoms like anxiety and depression, I would like to try them again but it’s just so hard to source here
Psychedelics are the reason why i didn’t take my life when i was at my end. I was stripped of my ego and saw the beauty of life and interconnectivity and even though i still battle anxiety and depression, I’m doing better everyday and will never think in such a self destructive way again.
LSD and mushrooms completely changed my whole outlook on life. I became a better version of myself
This experience gave me a lot of confidence about my self and my body. A bunch of bad thought / behavior patterns were broken. One of these was pretty bad OCD that made me wash my hands a lot. It gave me a lot of hope that things will be fine, this is the one thing that I heard throughout the trip: Everything is alright. The main reason for the trip was my severe depression and it definitely helped me (although it's not gone). Before all I could do was lay in bed. Now I am trying to rebuild my life one step at a time which wasn't possible before."
[_James_tray]
Got psychs
@@sarahh321 Where to search?? Is it IG?
@@Jerryberger9235 Yes
A wonderfull technic ... i ve learned from you about 1 year ago.. and i ve now many jars like yours and selling them .. Thank you my friend ✌
Awesome technique. I need that book.
You guys are awesome. I'm just getting into mycology and want to work for a mushroom company until I can start my own company. Valuable info.
Thank you for putting this out there it really helps everyone
Hello Mossy Creek mushrooms. I went to a location yesterday that was supposed to be monster mushroom company had to drive for a little while to get there I got there and the people were rude to me they did not have the thing that I wanted they did have some sterilized grain and some sterilized substrate so I bought a bag of sterilized substrate for $30 got home with it and come to find out there is a hole in it and where they tried to seal it there is a hole big enough for me to stick four fingers and and it goes straight down to the substrate so I did not get a bag of substrate that was sterilized I got a bag that was unsterilized really frustrated at this point and probably am going to be looking at what you guys have for sale.
:( Man, that sounds like a bad day. I hope your able to get what you need and well, it doesn't seem hard to beat the competition uh?
Great video your content is consistently top notch. Please keep up the good work. And thanks for sharing.
Making liquid culture tonight--inoculating it tomorrow! Thanks Mossy Creek Mushrooms. 😊Jen
Great content - I like the idea of the needle biopsies better than going from tissue to agar and then liquid culture. Less opportunity for contamination by exposure. Question: What is the best needle gage to collect a proper sample? Also, at what temperature do you store your liquid cultures while they propagate? I am using 1/64th teaspoon of peptone, 1/64th teaspoon of light malt extract, and 1/2 tablespoon of corn syrup with 600 ml of hot distilled water. What is your favorite recipe?
1) Put gloves on, spray gloves with alcohol
2) spray table, spray jars, spray the floor and the air for funsies until I flee slightly light headed from all the iso in the air
3)Spray my spray bottle with separate iso sprayer
4) Spray my hands again, spray the jars again
5) Spray the table one more time for good measure
6) Innoculate a jar or two
7) Spray the shit out of the table, and my hands again
8)Flood lab with 500 gallons liquid death
9) Drain lab
9)Continue work as per usual
Few questions
How often do LC jars need to sit on the magnetic stir plate? How long can they sit without being stirred?
Looks like the grey lids you're using are the new "leak proof" lids for mason jars. Just curious what are your thoughts on plastic mason lids vs metal? Rust aint fun to deal with but even Roger Rabit on the forums at the shroomery holds the opinion that plastic lids contaminate more. Seems unlikely you would be using them so much if that was your experience. Thoughts?
Once a day is good enough for stirring with magnetic stir bar. More would be better. As for lids plastic works just fine. Just tighten again after autoclave as they sometime get loose. The whole contam thing has prolly more to do with the filter disc getting too wet and bacteria getting in that way.
Great videos, very didactic. Thanks a lot.
Michel from Belgium, in Europe
Genius! Thanks for the idea. I guess there is no need for agar plates now :D
I love your channel i learn so much, i always thought LC was stirred to disperse the mycelium, sorta like how shaking grains at partial colonization to spread the mycelium better.
Wow, mind is blown! Can’t wait to try this out! Thank you so much from the bottom of my heart lol
nobody is talking about how cute hes and he got a lovely smile
How long should you stir for? Also once the culture is established, how long will it last on the shelf? How do you store it after the culture is established?
If someone is really OCD about sterilization they can put items under UV-C lamps as well as pressure sterilization ... I use them for my breathing masks and filters ...
Wow. I have always gone fruit to agar to second agar to liquid culture. Good to see this is possible. I will try direct to LC. I have some LC jars cooling right now. It is my first time using the grey leak proof lids. So far, so good.
Thank you guys!
Great video- I wish I could subscribe twice.
How long do you recommend each stirring session (if done daily) should be, if using a magnetic stir plate? 5min.? 15min.?
Btw, this is definitely my favorite cultivation channel as a total newbie. You're extremely thorough and give fantastic explanations, actually describing *why* things are done, rather than just saying "do this" because it's what others say to do.
Plus, I love Norse culture too (I see the Mjölnir necklace and the Icelandic magical stave on the door) so that's a bonus🤘🏻 Anyway, thanks for all your videos!!!
I don’t think you’re using too much alcohol let’s face it it’s better to be safe than sorry I’m just glad you’re not running sterile needles through a flame to make sure they’re sterile like so many other people do
Thats really cool I always go scalpel to agar to lc but I really like this for cloning. Gonna go for it! Thanks
Thanks for sharing; this is a very helpful lecture
i will definitely try this, Thank you
Thanks dude, I totally learned a new technique from you today! Thanks for sharing. I also purchased some liquid culture pre-mix (and used your code) from shroomsupply. I will give LC another go because my first try using Karo corn syrup was great at growing mycelium and mold no matter how clean my process was using a SAB. I also noticed when using your flow hood you should elevate your work surface just a bit so its not right at the very bottom of the flow hood. Anyway great vid, can't wait to try this out.
Big fan of your videos sir 👌👌👌👌👌 all my liquid culture is totally successful only because of u sir…
Love that room you have! I wish
Awesome technique. Beautiful. Can't wait to try it. I'm still relatively new to growing but recently I just used clean tweezers to pull some mycelium from a block and put it in lc. Totally worked. I'm stoked.
Very impressive!!! Well done!
I learn so much from YT videos. I now have another channel to add.
I appreciate sharing your secrets.
Glad you like them!
Great video
Spinning mycelium - "Jörmungandr twists and turns, mighty in his wrath..."
here's a few suggestions to try
pick the best type - there are many available.
grow them in the best environment - some eg oyster muchrooms like cool humid conditions and may be placed outside (I read these and the reasons they work from Gregs Mushroom Grower site )
The eyes are full of primal hate
O
Love you Andrew!! This vid been on my to watch list for a couple days. Great one
Thanks!
This looks a lot easier than anything else I've seen! Thanks for sharing! I have been told that DNA degeneration happens when you take tissue clones from clones. Have you found that to be a problem?
As a microbiologist/geneticist I can tell you that this is NOT a problem. You are not making a copy of a copy. The analogy that would be more fitting is you are taking a cutting from a tree that was grown from a cutting.
@@michaelgrouse7850 oh that makes more sense. So it's more like propagation rather then a clone thing. Ya when i think of clones i think of the star gate series with the Magvyer guy. The one alien species was like dying off cause they couldn't reproduce so they cloned themselves and was like dying off. Haha it's good there's real geneticists out there to counter hollywood and the vividness dilemma.
good explanation bro
WOW...I LOVE THE STOREROOM...AMAZING
Thanks for informative video! I will be trying this.
Great video.
Good methods promote growth, great video!
thx dude! greetings form poland!
Awesome video. Thanks for sharing.
My liquid culture solution is easy peezy. Gummy Bear TEC. I use 3% gummy bear by weight = roughly 1 bear per each 100 ml water. Organic Black Forest Bears are my preferred brand. There is a little gelatin in there as a protein source. The lower ph from acid and juice might reduce contamination?
Can you give me more info? Someone gave me a bunch of those gummies, and I want to use them before they turn funky, and that looks to be a nice, practical use. Do I just dissolve the little boogers in my water?
@@melhawk6284 Start with a ball jar with a liquid culture lid (and a stir bar if you have a magnetic stirrer). Add 3 grams gummy per every 100 grams water. Pressure cook the jar for 30 min at 15 psi. Cool. Inoculate. Stir the jar regularly while culture grows.
Awesome, engaging video
So cool. Thanks for your guide :)
Tunes.... Love it
Thanks!
thank you for the video. love your Mjolnir brother ;)
Best vid on the net!
Thank you for the info.
One of the best! Thanks mate
Glad you like it!
My big beard brother, you are genius 😎 like
I'm not sure how effective my attempt at the needle biopsy was on the first attempt. I'll try your twisting the needle in method on my next attempt.
Lashings of isopropyl - love it 🤣
I shot you an email about your thoughts on liquid culture jars and making your own. Did you see it? Additionally would be great if you could make a video explaining generation and how they apply to liquid culture. Maybe how you track them and how important is it to track them. Just a bit confused on that topic I guess. Keep up the GREAT work and THANK YOU!
I too am super interested in generation aspects. Bump!
Thirded!
Bro, you use iso like I use iso. I like it!!!!
This man's iso game is strong!!
Thanks man! great video
Thank you so much! I learned a lot of useful things for myself!
I love this idea..
Thanks for the video brother!
NP! Thanks for stopping by!
Fantastic videos! Thanks so much for all the effort and time sharing this valuable information.
What do you inoculate your Masters Mix bags with? Grain? or directly from the liquid culture? Liquid to grain to Masters Mix?
Thanks so much and please keep up the great videos!
Great video Bro, thanks for the info
I came up with the same process on my own, just searched to see if anyone else was using it--pretty cool. mine method is slightly different but essentially the same.
impeccable timing my good sir!
thanks for sharing
Nice Breakdown
Pressure cooker gets some alcohol too Hahahaha. First time watching it.
Hahahahaha irk XD
It's a game changer. May i know your failure rate compared to agar?
its all in the Twist '''''!!!!
If you're going to wipe it the mushroom down to get your tissue sample don't use alcohol use hydrogen peroxide
Great! I get more exp watching this. Thanks big man👍
Glad to hear it!
Thank you. Thiis is what I have been looking for.
I get my from an online store, they got shroom, psychedelic and microdosing products well refined, also ships to anywhere.
He's on Instagram with the below handle..
Mycopete.
from syria love you 😀🌹
Great video 📹 👍
Glad you enjoyed it
Hey bud.. I just wanted to say a couple of things. First.. yalls lab is BAD ASS! I am quite quite envious. I intend to be building one soon as well. Wont be as big as yours but.. if all goes right and the Good Lord Jesus wills it so. its gonna turn some heads :) If and when I build it, I may make a video to show you. And lastly.. I dont think you use too much alcohol at all actually. I think it is perfect. I plan to do it exaclyyy how you do it with the alcohol. Better to have a bunch of nothing go wrong, rather than use a little and all hell breaks lose. Keep up the good work bud! :)
Thanks! We appreciate the positivity! Good luck on your lab!
Awesome! Would it be possible to perform this same needle biopsy technique from the tissue of a dried/stored mushroom?
Chris Kustra this is a very good question indeed.
@@vinodpunj3102 I'm not expert but I would probably say no. If it's completely dry it probably doesn't have any actual living tissue to multiply itself from.
If you guys are still wondering, it does work. I ran the idea to a friend who has a certain interest in mycology. He gave me a few dehydrated (probably with cool air) samples that he had sitting around and forgot about for over a year and a half. I didn't have a needle at the time, but I used an exacto knife that was wiped down with alcohol and flame sterilized to carve a small chunk out, after exposing the center with the same process splitting it open like in this video. The other thing I did different was adding it to an agar recipe I learned instead of to liquid culture. Did a few more times in case of any possible contamination from the first, which I did have a couple contams, but with a lot more that were successful. Not sure if mushrooms dehydrated with heat would have any possibilities as well, or mycorrhizal varieties that usually need a plant host to survive. Will probably run tests on those theories eventually, but for now I am just excited with the success I had with this. And I hope it helps others out too.
Very good info,. 👍👍👍
Hello sir
Can you tell me How do you make liquid for this mushroom culture?
Thank you very much. What are the conditions (e.g temperature, light), that the jars need to be in while the mycelium is growing
My understanding, and I'm a complete noob, is a dark warm spot. I've seen 70-80 F is a good range
First I need that beard friend! This is cool I love earth