Mushroom Mycelium Tissue Culturing in Liquid and Agar Media | Southwest Mushrooms
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- เผยแพร่เมื่อ 20 ก.ย. 2024
- Here is our lab process for mycelium tissue culturing for both liquid and agar media. The most important part is cleanliness and a sterile environment to reduce risk of contamination. We do this every 2-4 weeks to ensure a healthy catalog of fast-growing mycelium strains at our farm.
www.southwestmushrooms.com
www.michaelcrowemycology.com
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▶ Cultivating fresh gourmet mushrooms at our urban grow farm. Mushrooms with amazing flavor and full of beneficial nutrients. Our passion is to offer every customer the freshest product in the market. Owner "Mushroom Mike" enjoys sharing the science of mycology while serving top chefs, everyday cooks, and those who enjoy a healthy lifestyle with premium mushrooms.
Hes just so dense with knowledge I could listen to this guy talk all day
Yes!
How long does all mushroom liquid cultures Syringes last in room temperatures and Refrigerator? Is growing dove Oyster mushrooms the same process as growing Chicken of the woods? Bye using rye grain and straw hay!
Yeah this guy is real dense
Yes me too, I listen until I get to sleep...like music to my ears
It's Basic mushroom growing knowledge. Just say he's dreamy...
you have the most savage flow hood setup man
I think it's 3'x4' panels. Jesus
Once upon a time... there was a perfect world...
In which I had this set up haha
1000%
He has a fantastic setup... but title of most savage probably belongs to someone else 🤣
Flow so good even the mushrooms knew what's up.
It is rather relaxing to hear you talk and there is a lot of useful information.
You Sir are truly the Bob Ross of mushroom growing!
Happy little mycelium clouds.
Hahaha you both are cool dudes
You can really tell that he is coming from the ground, building this up. All the og tips that we get from him, if we don’t have the proper equipment are very helpfull. Today i just started my mykology class in university and i‘m so excited to do all the experiments like i see in you’re videos. Couple months ago i saw a video from you and that got me hooked up to mushrooms, so thanks a lot!
what do you learn in mycology class in university, are you studying biology ?
Fungi!!
nice have you learned to spell mycology since?!
Happy shrooming! Great hobby!
@@NessieTheCatt 😂
I am a chemist with a degree in aromatic solvents. As such, i restore ancient works of art. Rarely do i run across people who REALLY KNOW their background and their science....to the core. You are one of those people. Bravo!!!!! Take advantage of this mans videos...they are rare insight👍👍👍👍👍
Giving out game like there is no tomorrow. Respect.
Mushrooms will help us traverse the universe not only mentally but physically.
Such a great overall nutritional source.
I absolutely love that when you say something is fun you show no indication that you're having fun haha
Thank you so much for sharing Mike, the details make all the difference and help to avoid numerous mistakes. There are few people with the willingness and ability to share this knowledge so well and so simply. Again, thank you very much. Hugs from Brazil.
man you designed your whole facility like a mad scientist! the bigger im getting and the more consultation based work I do, the more I wish I could have been apart of building the beautiful farm&facility you have at Southwest.. wicked!
My man! You are such an inspiration. "Started from the bottom, now we're here." All success to you.
His setup explains why I get anxiety every time I'm about to inoculate. You either have a NASA setup or live in mortal terror of the mold menace.
What are you working with? Flowhoods are great, but it really isn't strictly necessary for small scale growing.
@@superbarnie prob a shotgun chamber if he’s getting mold issues
Yep that sounds about how my first batch went. Mold mold
Mold
There are definitely in between options... i got a complete HEPA laminar setup and coupled it with a 2x4 foot shelf to make a big work space... i was willing to spend a bit more, but under 1000 dollars for the shelving unit and the complete fan filter and hood setup. If you want to find a cheap HEPA filter go check out Staples and you could easily get a 9x9inch filter for about 30 bux that you could use to make your own clean air box. Question is just how big of a space you want to work in and how much you want to spend. I was lost in my early research but realized there are options for every price point
@@SirCheese710 I wouldn't recommend using those HEPA air purifiers found in stores as a flowhood, generally their filters are not thick enough to create laminar air flow. IIRC, you need the filter element to be at least 6 inches deep for laminar air flow.
I'd suggest going the DIY route if you are low on funds and a flowhood is really necessary ( it usually isn't). A 2x4 foot flowhood can be made for around $600
I love this dude dont hold any knowledge back. Thx bro
Great video. I especially enjoyed the part at the end, when he's just talking about his favourite mushrooms to grow and substrates and what not while still doing transfers. Gives me confidence that maybe I can succeed in it too.
I've heard a lot of tripping stories, and they are very exciting,I would love to try magic mushrooms but I can't easily get some, Is there any realiable source I can purchase from??
I have been looking for a legit seller to buy from.
Yeah, I got mine from bergwilly1
On Insta
I have heard about him. Is he really legit?
The Trips I've been having have really helped me a lot,I finally feel in control of my emotions and my future and things that used to be mundane to me now seem incredible and full of nuance on top of that I'm way less driven by my ego and I have alot more empathy as well
“And in this next room, we have the Gamma-Ray Gun where we turn Humans into Mushrooms”.
Great videos, very impressive facility, loads of useful info. Cheers
Really, 🤔
@@ritahkays7410 Technically he is correct, you can turn humans into mushrooms using gamma rays...
But it is not a nice thing :D death by radiation sickness, then you use the body as food for fungi...
@@CMDRSweeper شببييثصجحج١2٢١
This was funnier than it probably should have been
Step away from the video games. Do it now
These are by far some of the best videos I have seen. Thanks so much!
If he was a teacher i could listento him all day without getting bored
your teaching skills and knowledge are amazing, thank you!!
literally can't blink while you speak following your teaching, and its amazing.insane process. I've watched this couple times already but i'm totally new to this topic.
Thanks for the time to teach.
Great video man - 'I am a vector of contamination' - best line ever....
Your videos have been invaluable to my university project looking into making mycelium based toys, thank you!
Likely one of the best mushroom videos out there. Thanks!
i love how you love what you do! the passion you have just oozes thru. thank you so much for sharing your love!💖
Amazing setup... Starting out just now .. changing my set-up from Cubes to Gourmet. New sub
lol sad.
Sick set-up, thanks for sharing, keep up the great work!
THANK YOU SO MUCH!!! Super fun video. I very much appreciate getting into the little details. Very interesting.
Dude, I wish you had a book or taught classes so I could get the whole process.
Hold tight, a lot more videos coming
@@SouthwestMushrooms can't wait!
Southwest Mushrooms start a paid website to learn rshrooms 101 102 ect ! You will set the standard . Get a Dagree from southwest mushrooms will be the valued standard .
@@SouthwestMushrooms do you sometimes leave a stir bar inside your liquid cultures?
@@jeremyhinken3365 thanks
Very informative and easy to listen to and understand! Thanks ! Take care! Peace ✌️ from Welland Ontario Canada 🇨🇦
2 years later but I'm so glad I found this vid/channel. So dayumm descriptive FINALLY!
I can tell you love this and you’re spreading the love ! Learning tons. Been looking for a way to grow my own food and came upon your channel and it’s opening my mind to even more possibilities. Thanks!
the cleanliness in his grow is something to behold
MAAAAAN THANK U FULL FOR I WAS WAITING FOR THIS VIDEO A LOOOONG TIME AGO, we use in Argentina an expresión to say thank you to the amazing people: sos un capo amigo🤙🏼🤙🏼🤙🏼
You are doing amazing work sir. You have imparted so much of knowledge and that too free of cost. You are an amzing teacher.
I already cultivate on a smaller scale but I'm expanding to a full size operation next year. Your videos give me some structure on how to setup my area. Also for someone that might be new to cultivating these videos are spot on and have invaluable information in the process so good job man. 10/10 great videos.
How is the scaling up of your operation going now? Just curious since I ran across this comment and its a year old ;)
I don't know how I came across this channel. But I'm so happy I did.
Lab jacket is a nice touch Mike 🙌🏻
These videos are amazing. So inspiring to a brand new grower.
My love of nature and the forest brought me together with mushrooms. And mushrooms became an obsession after that
outstanding demonstration, thank you so much!!!
I love the work you do for the advancement of mycology. I just wanted to make a mention of this.
Im unbelievably jealous of your setup
Impressive. Thank you for this! I've developed a passion for mycolocy and I have goals to start growing and producing mushrooms to help people. Good stuff brother! 🤞🏾
Magic Mushroom Mike's Masterpieces of Mycological Manufacturing Methods!!
Thanks for making these videos. DENSE with info, and he’s so pleasant to listen to 👍
Youre a great teacher. Thank you for sharing your knowledge!
Thank you so much for the video! Awesome video quality and excellent information explained to the level where the hobby mycologist can take it all in and not feel overwhelmed! Can't wait to see more!!
Phenomenal! Clear, easy to understand info. Thank you!
*Notice how WELL he disinfects everything*
I haven't watched a lot of your content yet, just found you recently. Very interesting content indeed and your stacked plate work was very good, I haven't worked in a micro lab for over 15 years so lovely to see. You may cover this in other videos bit in case you don't, your refering to aseptic technique when you talk about maintaining sterility in a lab, I thought that might help some people who want to research further about it. (For the you, it's not important, as you clearly know what you're doing).
Also for others the plates come in the bag, sealed for sterility, if you open carefully it perfect to then store them in again, with a little masking tape to close up. I'd only store them like that, if storing blank plates (Petri dish with set agar) before use. I'd tend to use cages to store inoculated plates, but they lived in climate controlled cabinets.
Also interesting fact about agar for those new to it, you heat it up to about 85°C (about 185°F) to liquify it and it will then stay a liquid as it cooos to around 37°C (about 100°F) then it gradually set.
Brother each and every video of yours is a gem
i dont even like mushrooms, but i cant stop watching all your steps on how to make them!
This is not as popular as it needs to be. Good job man.
This guy is awesome. Keep spreading those spores!
loved how in min 2:39 the calendar on his right (our left) marks the month of FEB and this was posted on June
Awesome, engaging video
Your operation is incredible.
Who ever helps you with camera 🎥 work is the bomb . 👋🏼 five great job ! Say hello some time !
The most educational and informative instructional video on raising mushrooms I have watched. Thank you. Really like your sincerity. Very gratifying.
Came from paragraphic watching all your videos and subscribing now!
He does a great job explaining how this all works in terms that I can understand
Another BRILLIANT video.
So much here that I don't understand... But as a teacher, I can recognize another teacher, and I'm watching a great teacher here. Keep it up man, I can sense that you're on a very valuable mission
gee you guys make it look sooooo easy :)
he is definitely a man of culture
Waw, you are such a great teacher. I find your tutorial as the more practical. Thank you very much for sharing your knowledge. Keep up with the good work...
Michel from Belgium.
I wonder if he would be willing to be a mentor?
I really love hearing about these kind of things (even though I'll probably never start a culture)
love this man thank you !!!
great setup!!!
Flo hood Jesus. Beautiful video , jealous of your setup , appreciate this video. You're a skilled teacher , mad respect my dude.
Hey, Im in Phx Az as well! Im glad to know you are here in the valley with me!
PHOENIX! Finally a grower in az. I would love to work with you man you seem like an awesome teacher.
I would follow you with a camera like a zen student. That space is beyond awesome.
Peace to the Gods, man. Say brotha, let me first thank you and give you your roses. You are well spoken, intelligent, and thorough with your explanations. I've watched a good amount of your videos and I am beyond elated and impressed. I just recently started my journey in mycology, and yes, am very fascinated with the world of fungi. You have been doing this for a long time and your knowledge shows. From every fiber in me, please continue to work your magic...please continue to study and soak up as much as you can, so that you may further your journey as one of Earth's messengers...please continue to be a good father, and if you haven't been, it's never too late to change...and always give thanks to our ancestry and those who came before you, for without them, none of us would be where we are today. Thank you again, king, and Happy growing 🍄🟫🍄💯
How can you tell when your LC has reached it's optimal viscosity? Love your vids!
"I'm a vector of contamination, I'm the dirtiest thing in my lab".
yea,... hot
Yeah, No mask. No hat.
@@timplace2513 let me know if I'm wrong but isn't that what the flow hood is for to blow air towards the cultures and then him then it gets recirculated through the hepa filter before it goes back to the cultures?
@@azbanbo But it's possible while talking that spit might fly into it no?
Actually the laminar flow hood, blows air horizontally or vertically unidirectionally. In this case it's horizontally towards him, and away from the cultures. Kind of like a fan, and doesn't get recycled.
Congratulations and also I Appreciate your Interests and Loves.
Nice Fungies
Amazing quality videos! I have picking up tips and look forward to even more.
U truly are one smart King love the content keep shining like u do
this setup is incedible
Your videos are amazing my dude.. Rookie mycologist myself. I find alot of great content highly useful in my endeavors. Thank you very much.
After listening to him talk, I realized that knowledge is something that should be shared for the prosperity of others, not for profit. He’s such a genuine person that I cannot fathom how someone intelligent could possibly dislike this....
This video is chalk full of information to study and experiment, he’s trying his hardest to make it comprehensive and interesting for the sake of entertainment
Yet at the same time, it’s his honest interest i life, because it’s such a staple within life and healthy development within life.
I personally have so much admiration for his career, I fully support it emotionally and I’ll try to support it financially. He studied and it shows
You can see that there’s a though process to each and every process, yes there might be a reason for each process, and logically here might be a better process. However, for some reason we stick to the same process, and that’s usually because it works best (most efficiently for the goal set)
I’m not trying to be biased, but I also love in Arizona; I also have an agreement through personally experience that Arizona can be a very good place to inhabit for mushrooms
Thank you for your ultimate videos. Sharing your knowledge with others is a science of the wise.
You are a good teacher and thanks for sharing the finer points in your videos.
Really great vid! Just what i needed to see. And yes it’s a bit intimidating with al the sterile stuff as a beginner. I really want to start my own little mushroom farm. And this helped a lot! Exited to see more
You can certainly do it! My wife and I started a mushroom farm over the past year. It is challenging, especially if you encounter a lot of trial and error, but this is something worth it and very rewarding.
@@familyfungi that sounds so nice! How has the experience been so far? Did you require a lot of space? And do you grow indoors or outdoors?
@@vishnu2407 We grow mostly indoors, but some outdoors. We live in South Louisiana, U.S., so its challenging to grow outside in our hot summers.
Our lab (clean room) is 10 x 16 ft. And our fruiting space is the same. So that's 320 sq. ft. total, however it would be more helpful to have around 1000 sq. ft. with a dedicated space for colonization.
Relaxing to watch and listen to the technicalities. Interesting and fun to learn such things. Seems like a neat person all around. Chill person to work for and with. I'd look forward to going to work every day in such a place.
Thank you for this great video! I happened to be doing this for the first time TODAY!!! (Grain to grain.) What awesome timing. 🙏
Massive thank you and big love for sharing such incredible knowledge ! Much appreciated :) !
Psychedelics have proven very effective in the treatment of various mental health issues asides other health benefits. Helped me get out of years of depression and excessive alcohol use.
was having this constant, unbearable anxiety due to work stress. Not until I came across dr.lucasmushroom a very intelligent mycologist. He saved my life honestly
Yes..dr.lucasmushroom
@@rosemary8305 Yes..dr.lucasmushroom
Is he on insta??
You remind me of one of my friends. Very inspirational as far as trying to be clean im going to need a flow hood one day
It looks so clean, I dont even want to cough on the screen!! :)
Thank you so much for all these little tips and tricks! Love it
Thanks for all the great videos!!
On watching the mixing process I think random alternating spin direction would give a better breakup and mixing. Great to see the processing involved! Thanks
You are a wonderful teacher, I could listen to you all damn day
Sweet Shitaki I always thought that I had a badass education this Professor is the Bomb
Thanks man! Great set up and simple explanations. One question: Why is liquid culture inoculatation not good for substrate (e.g. sawdust substrate)?
I'm a year late but screw it, nobody's answered and you've got a few upvotes. So the mycelium in the culture is forming very loose networks in the nutrient solution that are easily broken apart when transferring, but with grain, it has a solid source of nutrients to form a concrete network on so it will have a much easier time branching out and colonizing a new substrate from there. It's like if you dropped people randomly into a forest they would eventually meet up and pool resources and could form a society eventually but they would have a much better chance if they were dropped in as groups that are already working together and well supplied.
Continuing with the analogy, these groups of people would also have a better chance of surviving a lone wolf attack than a lone person would against a pack of wolves, so you're gonna want to thin out the predators next. With grain you can work in small batches, it is nutrient-rich and you can sterilize them so your mycelium has no competition and can easily become established. With bulk substrates, pasteurization is commonly used by beginners because A) it's difficult to properly sterilize large quantities of a substance without a large (and expensive), industrial autoclave and B) pasteurization allows a few organisms to remain to keep each other in check so one doesn't get the upper hand over the other. Then when you drop in well-established networks of mycelium special forces can easily overpower the scattered wolves or nematodes or Trichoderma or whatever and take over the place
Edit: There are other reasons for the culture to grain, grain to bulk substrate process too. For example, some species will not fruit on grain and need a wood diet in order to reproduce. Other species, like cordyceps sinensis, only grow on insects. So why are you putting them in grain spawn? That won't work. You just look silly
@Eleventh Monkey Gaming I personally use 25 ml of honey to one liter of water, but malt extract and several other things can work. If you want a lot of great details about LC prep and recipes I'd recommend Agar and Cultures part 4 by the Humble Fungus
Great work! Congrats
I didn't know things can get this professional for a mushroom grower 🐿 looks like a lab
@Raul Cano EScuder I like the plant based products
How muvh do you sell and how do you sell them?
I usually check NorthSpore out
Amazing video. Thanks for sharing your knowledge and calm attitude.
Got a love those gorilla grow tents. I got three myself and I wouldn’t buy any other brand. watched a friend brake a tent Because the plastic frame. I that will have them for years. They’re built to last us for sure and I’m not getting paid for this so back to the show folks! This guy is Awesome so knowledgable.Thanks for the knowledge dude.