It is important to note that oxaloacetate cannot cross outside the mitochondrion. It has to be converted to Malate, which will be transported to the cytosol via Malate-Aspartate Shuttle. Malate (now in cytosol) is then converted back to oxaloacetate (because of high amount of NAD+ in the cytosol) allowing phosphoenolpyruvate kinase to catalyze it into phosphoenol pyruvate. 😊
4:38 The enzyme used to convert Phosphonenolpyruvate to Pyruvate is Pyruvate Kinase not PFK1. It's written in biochem textbooks and can also be found online when you search for step 10 of glycolysis
very helpful and simplifying, but please avoid mistakes such as the pfk mistake in the step 10 of glycolysis. this could be misleading for students who are having an exam. at least put a correction in the comments or the discription.
Sir I am watching your channel from last 1year I really love the way you share the topics to us,becouse you have been teaching us sir I have started my channel on TH-cam just getting inspiring from you i started my channel last weak only,I really thank you a lot for making me inspire sir.
You should also mention that Glucose to G6P is converted by Glucokinse/Hexokinase as Glucokinase is only present in hepatocytes and B-cells of pancreas.
I think glucokinase which converts Glucose to Glucose 6 phosphate, only can be found in the Liver. Usually, Glycolysis can occur in all cells, so Hexokinase is the one that would replace glucokinase.
Glucose is converted to G6P by enzyme Kinase and it's called the phosphorylation step(1st step) and ADP is converted to ATP as it is energy investment phase with the cofactor Mg2+
You should redo this video with the corrections because it really is very good on how your layout things so simply. It is excellent so I suggest you re- do the video with corrections.
Oxaloacetate is impermeable to mitochondrial membrane it is converted to malate and then transported to cytosol and oxaloacetate is regenerated by malate dehydrogenase enzyme
I have a doubt. When glycerol is used as a substrate, gycerol-3-phosphate is formed and it gets converted to dihydroxy acetone phosphate for reverse reaction. Can't the glycerol-3-phosphate directly participate in the reverse reaction?
I was under the impression that gluconeogenesis could be classified as the creation of new glucose from non-carbohydrate sources - i.e., shuttling fructose into gluconeogenesis at the liver so it can be released to the rest of the peripheral organs as glucose.
How often and in what amounts does this process actually happen during fasting? I've hear of gluconeogenesis happening with amino acids, but I think fatty acids actually convert to ketone bodies, not glucose, during fasting that lasts more than 24-48 hours. I'm willing to be that this reversal of pyruvate back to glucose happens on a very small scale, or maybe a temporary emergency situation when glyocogen stores are extremely low. I'm also willing to bet that muscle tissue used for energy is low as well during a fast. It only makes sense that during fasting, the body is smart enough to utilize the stored fuel in adipose tissue as it's primary source.
Also, when it comes to fatty acid metabolism; two glycerol backbones of triglycerides can form one glucose molecule. Perhaps this is what you mean't by fatty acids being converted into glucose. This form of gluconeogenesis happens on a small scale as well, but it does portent that dietary carbohydrate intake may be completely unnecessary.
When phosphoenol pyruvate will convert to pyruvate enzyme should pyruvate kinase......you are saying phosphofructokinase So can you plzz guide what it should be sir...
It is important to note that oxaloacetate cannot cross outside the mitochondrion. It has to be converted to Malate, which will be transported to the cytosol via Malate-Aspartate Shuttle. Malate (now in cytosol) is then converted back to oxaloacetate (because of high amount of NAD+ in the cytosol) allowing phosphoenolpyruvate kinase to catalyze it into phosphoenol pyruvate. 😊
Thnxx for the information ❤
Thank you very much
Ok I'm here to learn a bit about a low carb high fat diet and I have absolutely no idea what you're talking about 🤣
Very clear description. Thank you
True Aiza....thumbs up 😌 for being keen
4:38 The enzyme used to convert Phosphonenolpyruvate to Pyruvate is Pyruvate Kinase not PFK1. It's written in biochem textbooks and can also be found online when you search for step 10 of glycolysis
100%
Yeah..fuck this video.
Yes bro you are correct actually small mistakes by him during editing,and copy and paste mechanism
Yes
you're right.
Hexokinase also converts glucose to glucose 6 phosphate. Glucokinase does the same job but specifically in the liver. Thanks for the video 👍
Glucokinase is 4th form of hexokinase. So you can also say it hexokinase.
@@Natasha_Mirza yeah i was about to reply with that
very helpful and simplifying, but please avoid mistakes such as the pfk mistake in the step 10 of glycolysis. this could be misleading for students who are having an exam. at least put a correction in the comments or the discription.
no donations no corrections,correct ion can be done at glucokinase/hexokinase,phosphofructokinase/pyruvate kinase, malate/oxaloacetate shunt. huh.
I'm a Masters student in Biomedical Science and this has helped me way more more my lectures have. THANK YOU.
wait the enzyme which converts PEP to pyruvate is pyruvate kinase not phophorfructokinase
u ar correct..its not PFK
yes you are right!!
terrible error!
Saria Sultan good eye, good eye
I saw it even me right now
I saw it and I rolled down to see an explanation and here you are lol
Sir I am watching your channel from last 1year I really love the way you share the topics to us,becouse you have been teaching us sir I have started my channel on TH-cam just getting inspiring from you i started my channel last weak only,I really thank you a lot for making me inspire sir.
Channel name ?
4: 38 it is Pyruvate kinase not phosphofructokinase. BTW good 👍video.
4:38
Pyruvate-->Acetyl coA-->OAA-->Malate(crosses mitochondria) ---> PEP ( in the cytosol)
Your channel makes my concepts always clear. This is really superb way to understand anyone.
Very helpful. Explain the topic in a very crisp and profound manner.
Thank you.
Found it on the last day of preparation. Helps a lot :)
Can you make me understand....in more simplified way..
I like your videos. They are short and easy but please don't make mistakes in enzymes or steps.
ههههههههههههههههههههه صح
Thank you so much sir from Maharashtra
Thank you very much sir. ❤ from Sri Lanka
Most welcome!
You should also mention that Glucose to G6P is converted by Glucokinse/Hexokinase as Glucokinase is only present in hepatocytes and B-cells of pancreas.
And also...the oxaloacetate inside the mitochondria is first converted to mate which is transferred to cytoplasm where it again become oxaloacetate
I think glucokinase which converts Glucose to Glucose 6 phosphate, only can be found in the Liver. Usually, Glycolysis can occur in all cells, so Hexokinase is the one that would replace glucokinase.
Glucose is converted to G6P by enzyme Kinase and it's called the phosphorylation step(1st step) and ADP is converted to ATP as it is energy investment phase with the cofactor Mg2+
From somalia thnks , for u lecture, it is very nice
thanku so much. this video helped me to understand gluconeogenesis.
Best explanation in short time every found in internet. Thanks for explaining once more about Glycolysis
Itz. Amazing Lecture __100% understanding thnks to MedSimplified
Such a great videos...splendid..
May u get success in every field.. Plz do it on anothers topics of biochemitry.
You should redo this video with the corrections because it really is very good on how your layout things so simply. It is excellent so I suggest you re- do the video with corrections.
I took your advise to the heart brother. New videos on carbohydrate metabolism coming in a few weeks ok. 😉😉😉😉😉😉
MEDSimplified thank you for teaching
TQ short and relavent for mbbs
Oxaloacetate is impermeable to mitochondrial membrane it is converted to malate and then transported to cytosol and oxaloacetate is regenerated by malate dehydrogenase enzyme
Thankyou soo much !!!!! U really help in understanding biochemistry easily 💛
best channel ever
Verithanam 🔥🌟
Thaaank you very much
I like your videos so much
Best wishes
Thanks a lot sir...Its really very helpful.. 💖 JazakAllah JazakAllah 🙏
cute you are muslim
Dear Sir, your videos are excellent. Please make a video on glycogenolysis also.
Thankyou ! Great revision for my metabolism exam 😄
+TheMGPforlife most welcome
sir this video is helpful for mbbs ?
You explain in the simplest way possible..just one request please try to cover the portion in details
man this stuff is like better than khan academy, very nice work
After from mitochondria where the oxalo go?
The third irreversible enzyme is pyruvate kinase
Great explanation
This is the best I have ever seen
I have a doubt. When glycerol is used as a substrate, gycerol-3-phosphate is formed and it gets converted to dihydroxy acetone phosphate for reverse reaction. Can't the glycerol-3-phosphate directly participate in the reverse reaction?
Great outline of this pathway sir👏👏
very useful video at last I got it thank u so much medsimplified...
very easy to understand the concepts and steps...pls add more topics
Wah!!! What explanation it easy to understand and write by own thanks alot sir ur explanation is superb 🙏
Thanks it's very sufficient and helpful
انت مبدع شكرا جزيلا لك from yemen thanks
I was under the impression that gluconeogenesis could be classified as the creation of new glucose from non-carbohydrate sources - i.e., shuttling fructose into gluconeogenesis at the liver so it can be released to the rest of the peripheral organs as glucose.
thank you for this!! saving me in my macro metabolism class!
The best explanation 👍🏻👍🏻👍🏻👍🏻👍🏻👍🏻👍🏻
Please make a video on pentose phosphate pathway
Enzyme that converts phosphoenol pyruvate to pyruvate is pyruvate kinase
this channel helped me ALOT , much thx for u
This video help me in my project 😊
It's Pyruvate Kinase which converts PEP to Pyruvate in glycolysis. By mistaken it was written Phosphofructokinase.
How often and in what amounts does this process actually happen during fasting? I've hear of gluconeogenesis happening with amino acids, but I think fatty acids actually convert to ketone bodies, not glucose, during fasting that lasts more than 24-48 hours. I'm willing to be that this reversal of pyruvate back to glucose happens on a very small scale, or maybe a temporary emergency situation when glyocogen stores are extremely low. I'm also willing to bet that muscle tissue used for energy is low as well during a fast. It only makes sense that during fasting, the body is smart enough to utilize the stored fuel in adipose tissue as it's primary source.
Also, when it comes to fatty acid metabolism; two glycerol backbones of triglycerides can form one glucose molecule. Perhaps this is what you mean't by fatty acids being converted into glucose. This form of gluconeogenesis happens on a small scale as well, but it does portent that dietary carbohydrate intake may be completely unnecessary.
Made it clear , voice was good too ☺
Please make the video on HMP shunt pathway!
I like your videos sir. These are helpful
very nice. also glycerol part of fat and not fat is converted to glucose
HELPED ME A LOT THANKS MAN!
Thank you.
Best teaching method 👍
But nice video
Thank you so much
When phosphoenol pyruvate will convert to pyruvate enzyme should pyruvate kinase......you are saying phosphofructokinase
So can you plzz guide what it should be sir...
Thank you❤ so much 😊
Thnx dude got the notes for gluconeogenisis😉
Why did you not discuss Regulation of gluconeogenesis?
Great work broda.👏👏😍
Very Good 🙂
Please make a video on HMP shunt, your videos are just easy and good
Thank you
7 minutes video worth more than my lecturer slides lmaooo
i was confused but after 7 minutes I now understand this process
thamisanqa deputy lmao what about the 04
Thank u so much sir....all ur videos are really awesome and i can understand well....can u please make a video on HMP shunt pathway ??
Very helpful
THE LAST ENZYME CATALYZING THE IRREVIRSIBLE STEP IS PYRUVATE KINASE PLEASE NOT PHOSPHOFRUCTOKINASE...
Phosphoenopyruvate is conveted to pyruvate by the enzyme Enolase not phosphofructokinase
Thank you sir 🙏
Your lec are best ... 👍👍
qn excellent work
Your videos are super 👌nice.
Put videos for Amino acid metabolism and lipid metabolism. Fast As soon as possible.
Please make video on Glyoxylate pathway
Thank you sir
Can you upload glycogenesis, glycolysis, etc and hmp shunt?
+Aishwarya Birje U can find the video on glycolysis but j our channel. Glycogenesis and hmp shunt will also be uploaded soon mam
MEDSimplified Glycogenolysis* 👅 my bad!
MEDSimplified and thanks!
MEDSimplified hmp shunt pathway ?
4:55 pyruvate is directly converted to oxaloacetate or is first converted to acytyl coA then oxaloacetate as in krebs cycle
Please upload protein metabolism..nucleic acid metabolism video...
it,s very simplicity thank you
NIce Explanation Thanks
Superb sir thanks very much
*Pyruvate Kinase
Thank u very much.
It is a nice video .
Try to make hmp shunt also.
I was trying to learn about fat being burned through gluconeogenesis
please make a video on cori cycle
It really helped me
Thanks
Please make more videos of biochemistry like on HMP shunt ,proteins metabolism , ketone bodies etc
Thanku so much sir
Plss Upload video on pentose sugar pathway, nitrogen metabolism and nucleic acid metabolism it will be helpful for my exam