Our SOP has us washing an aliquot of cord blood four times then making a cell suspension. I do this by labelling two glass tubes with patient info, adding 3 drops of cord blood to each tube, washing them 4 times in cell washer. Next I will add about 1/4 of saline to one tube, resuspending the cell button then transferring that cell suspension to the other tube and resuspending that button with the first cell suspension. This gets me to a 3 percent suspension that has a decent working volume for forward and DAT along with weak D testing.
JonBoat Bill DAT (direct antiglobulin test) tests for antibodies bound to RBCs in vivo (in the body) which would be present in hemolytic disease of a newborn (HDN) or a hemolytic transfusion reaction.
7 years later and I still found this very useful! Thank you! 👍
Thank u..its been a long time i did not do manual procedure..all in middle east need to to do with machine
Thank you ... it’s informative & brief
Nice and to the point. Great video, I wish I watched this before my blood bank clinical rotation yesterday it would have been a lot easier...
Thank you, I’m glad it was helpful.
You are amazing plss do more videos
Our SOP has us washing an aliquot of cord blood four times then making a cell suspension. I do this by labelling two glass tubes with patient info, adding 3 drops of cord blood to each tube, washing them 4 times in cell washer. Next I will add about 1/4 of saline to one tube, resuspending the cell button then transferring that cell suspension to the other tube and resuspending that button with the first cell suspension. This gets me to a 3 percent suspension that has a decent working volume for forward and DAT along with weak D testing.
Just looked at a disposable pipet :) more like 1/2 to 3/4 ml of saline to resuspend cell button
Would love to see one on manual body fluid counts.
That’s a great idea. I’ll have to try and rig a microscope to make it happen.
what does a DAT test for?
JonBoat Bill DAT (direct antiglobulin test) tests for antibodies bound to RBCs in vivo (in the body) which would be present in hemolytic disease of a newborn (HDN) or a hemolytic transfusion reaction.
Robert Bounds thanks Robert I really enjoy your videos you're very informative.