im a biochemist. but has been posted to incharge for haematology laboratory. now im been ordered to incharge blood bank too. and tomorrow i need to attend a workshop of immunohematology. i know nothing about BB because just take over recently. now im watching this video to prepare myself. im biochemist. then im biomedic. now? i dunno.. so many job description in my 1 job title. the F.
For the Rh control tube, was the first bottle given by the manufacturer? I dont remember doing it like that in my immunohematology lab. Cant we also do Rh control with 1 drop patients suspesnion, 1 drop saline and 1 drop albumin?
+fah moh Rh reagent is basically protein with anti-D antibody. Rh control reagent is just protein. The Rh control is meant to detect a false positive in the Rh tube caused by the protein. Yes the Rh control comes from the manufacturer.
I have a question? My professor told me not to shake my tubes too aggressively because i could break up the button and give a false reading on the agglutination reaction (for instance a 2+ instead of a 4+). On Board exam questions, I've seen them ask questions on the discrepancies of forward and reverse typing, in particular the A group because of possible A2 cells. Are you shaking your tube too much to break that button up and how can i get a better understanding of forward and reverse discrepancies? Thank you so much
Yes, one has to be cautious when getting the button off the bottom of the tube. There are different ways of doing this, so you will have to find what works best for you. See my video Tube Reaction Grading for examples.Regarding ABO discrepancies, I break them into three categories: missing reactions, unexpected reactions and weak reactions. I believe most blood bank textbook should address this. There certainly are many videos addressing this as well.
@@patricktracy9947 thank you so much, I appreciate the quick response and the wonderful feedback. Truly has been a pleasure watching and learning from these videos.
Sir, my question about temperature. Which temprature is best for blood grouping test? Rh factor need 37c and ABO group are need 4c or 26c room temperature. Why they need difference temperature for perfect reading
We do the reverse-group testing to confirm the forward-group testing. Sometimes there are discrepancies between the forward and reverse, which need to be investigated.
Concurso, I cannot advise what your lab should do. Some blood bankers wash the RBCs before testing, but some don't. When I do testing, I wash the RBCs.
Hi, nice videos thank you , but can you explain the reaction when you are performing video. The reaction is what i'm not super understanding, when the anti-A gave a 4+ and the Anti-B 0 and the A1cell 0 and B cells 2+ is the pt Type A blood group ?
+Shanelle Pou Antibody identification is mainly done when someone is going to receive blood. In order to safely give the person blood, we have to ensure that they don't have any antibodies that will attack the blood. If they do have antibodies to the blood and we give it to them, it can have disastrous results. Therefore, detecting and identifying antibodies is extremely important.Another situation when antibody ID occurs is in pregnant women. If a mother has antibodies against the baby's blood and if those antibodies can cross the placenta, again it can have disastrous results.
Hello sir, i just came across your video, i hope you reply to my video.. A little bit worry because its an old video so i dont know if you still can read my comment. Sir, I want to know about the tube that labelled C. what kind of reagent put in there?( im not hearing it well. English is not my 1st language tho) You said it was for control. Can you explain a bit about that control..I dont understand.
Hi Miera, The control is for the D test. In the past, there were false positives with the D reagent because of the protein in it. The D control reagent is the D reagent without any antibodies. The control must be negative for the D test to be valid.
cool black gloves:) thanks for making these videos, they're a perfect review before clinicals
+Constance Spoor Thanks Constance :)
Hey thanks for the video Patrick, that was very educating.............needed to refresh my mind on that
Glad I found you
Thx from India dear sir
im a biochemist. but has been posted to incharge for haematology laboratory. now im been ordered to incharge blood bank too. and tomorrow i need to attend a workshop of immunohematology. i know nothing about BB because just take over recently. now im watching this video to prepare myself. im biochemist. then im biomedic. now? i dunno.. so many job description in my 1 job title. the F.
Hi Fariz,That sounds very challenging. I wish you luck in your new responsibilities.Patrick
@@patricktracy9947 thank you for your wishes.. :)
Thank you for the information. To tell you the honest true am lost. I only use the Anti D AntiA and Anti B in class
Do you use the A1 and B reagent cells for back typing?
nice one
For the Rh control tube, was the first bottle given by the manufacturer? I dont remember doing it like that in my immunohematology lab. Cant we also do Rh control with 1 drop patients suspesnion, 1 drop saline and 1 drop albumin?
+fah moh
Rh reagent is basically protein with anti-D antibody. Rh control reagent is just protein. The Rh control is meant to detect a false positive in the Rh tube caused by the protein. Yes the Rh control comes from the manufacturer.
Hi sir, what brand of grouping products are you using?
Great really thx sir
Thank you Sanjay.
That's very good. Thank you so much.
Thank you...I am glad it was helpful.
I have a question?
My professor told me not to shake my tubes too aggressively because i could break up the button and give a false reading on the agglutination reaction (for instance a 2+ instead of a 4+).
On Board exam questions, I've seen them ask questions on the discrepancies of forward and reverse typing, in particular the A group because of possible A2 cells. Are you shaking your tube too much to break that button up and how can i get a better understanding of forward and reverse discrepancies?
Thank you so much
Yes, one has to be cautious when getting the button off the bottom of the tube. There are different ways of doing this, so you will have to find what works best for you. See my video Tube Reaction Grading for examples.Regarding ABO discrepancies, I break them into three categories: missing reactions, unexpected reactions and weak reactions. I believe most blood bank textbook should address this. There certainly are many videos addressing this as well.
@@patricktracy9947 thank you so much, I appreciate the quick response and the wonderful feedback. Truly has been a pleasure watching and learning from these videos.
follow slow shaking ,if yu not shake you give false positive
Sir, my question about temperature. Which temprature is best for blood grouping test? Rh factor need 37c and ABO group are need 4c or 26c room temperature. Why they need difference temperature for perfect reading
Rizwan Riz because anti Rh is IgG but anti ABO is IgM,
I just have one question
What is benefit from reverse group like A1cells and A2 and B
We do the reverse-group testing to confirm the forward-group testing. Sometimes there are discrepancies between the forward and reverse, which need to be investigated.
Thanks man
And we will wait for more videos relates blood transfusions
Have good one
If my lab has no A1 and B cells to do the reverse test, should I wash the patient cells before doing the forward-group testing?
Concurso, I cannot advise what your lab should do. Some blood bankers wash the RBCs before testing, but some don't. When I do testing, I wash the RBCs.
@@patricktracy9947 Thanks. Another question: Do I really need to prepare a 3-5% suspension? Can't I just use 1 drop of the whole blood instead?
@@concursoebserh1322You must make a 3-5% RBC suspension.
Thanks, can u also do a video on g6pd?
Sorry, I probably won't make that.
Hi, nice videos thank you , but can you explain the reaction when you are performing video. The reaction is what i'm not super understanding, when the anti-A gave a 4+ and the Anti-B 0 and the A1cell 0 and B cells 2+ is the pt Type A blood group ?
In your example, do you not understand why the reactions you gave are
for a type-A blood group because the reaction with the B cells is only
2+?
No, sorry I was just confusing myself I talked to my professor and have figured it out. Thank you again!!!!
Good!
Explain antibody identification. Why we do it. Step by step procedure
+Shanelle Pou Antibody identification is mainly done when someone is going to receive blood. In order to safely give the person blood, we have to ensure that they don't have any antibodies that will attack the blood. If they do have antibodies to the blood and we give it to them, it can have disastrous results. Therefore, detecting and identifying antibodies is extremely important.Another situation when antibody ID occurs is in pregnant women. If a mother has antibodies against the baby's blood and if those antibodies can cross the placenta, again it can have disastrous results.
Hello sir, i just came across your video, i hope you reply to my video.. A little bit worry because its an old video so i dont know if you still can read my comment. Sir, I want to know about the tube that labelled C. what kind of reagent put in there?( im not hearing it well. English is not my 1st language tho) You said it was for control. Can you explain a bit about that control..I dont understand.
Hi Miera, The control is for the D test. In the past, there were false positives with the D reagent because of the protein in it. The D control reagent is the D reagent without any antibodies. The control must be negative for the D test to be valid.
@@patricktracy9947 Aaa okay sir. Thank you🤗
Do you have a website where you post the steps?
Davion, send me an email and I will send you the procedure. ptracy@wvc.edu
where from i get Antisera.
From a manufacturer.
More videos!!!!!!!!!!
+Shanelle Pou
What kind of videos?
What that adding cc called in red Bootle
Munef, I am sorry but I can't clearly understand what you are asking. I cannot answer the questions if I do not understand them.
Can I have these notes emailed to me?
Email me at ptracy@wvc.edu