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I thought with C3d you have to let the tubes sit for 5 mins at RT to allow complement to bind.
Thanks to you
This is a good job
I'm wondering if there is an issue with the cell washer after seeing your AHG results.
I was thinking that also. I probably should have spun them and dumped the supernatant off before adding the AHG. There wasn't that much left in the tubes but that was all I could think could have been wrong.
Good job
I thought with C3d you have to let the tubes sit for 5 mins at RT to allow complement to bind.
Thanks to you
This is a good job
I'm wondering if there is an issue with the cell washer after seeing your AHG results.
I was thinking that also. I probably should have spun them and dumped the supernatant off before adding the AHG. There wasn't that much left in the tubes but that was all I could think could have been wrong.
Good job