Patrick Tracy I was looking for 2+ mixed field because it's hard for me to separate them from normal 1+ or normal 2+. I thought I could grade 2+ mixed field by using microscopic examination only?
I am not aware that there are different grades for mixed-field agglutination. When we see it, we report it out as mixed-field. The way we differentiate mixed-field agglutination from a regular 1 or 2+ reaction is as follows: In a 1 or 2+ reaction we will have several smaller clumps and a cloudy background. With mixed-field we will probably have just one larger clump and a cloudy background. For example, let's say a B patient received O units. When we forward type that patient's RBCs after the transfusions, the anti-B reagent will cause agglutination with the patient's own B cells resulting in the one clump. The transfused O cells will not agglutinate with the anti-B reagent and therefore will remain free causing a cloudy background.
![อยู่คนเดียวกับเขา - ส้ม มารี (Zom Marie) [Official MV]](http://i.ytimg.com/vi/gq1cWAIQDLg/mqdefault.jpg)
I am in a CLS program in Texas and your videos are VERY helplful.
THANK YOU!
+Selene Sosa You're welcome.
Hil
You should definitely do a video on determining what blood type a person is.
+Jessie Molly Hi Jessie,I think that's what the ABO/Rh video does.
What's the different between 2+mixed field and normal 2+?
Do you know what mixed-field agglutination is? Watch my video Mixed-field Agglutination.
In the Mixed field agglutination video you only demonstrated a 4+mf reaction and did not address other reactions.
Patrick Tracy I was looking for 2+ mixed field because it's hard for me to separate them from normal 1+ or normal 2+. I thought I could grade 2+ mixed field by using microscopic examination only?
I am not aware that there are different grades for mixed-field agglutination. When we see it, we report it out as mixed-field. The way we differentiate mixed-field agglutination from a regular 1 or 2+ reaction is as follows: In a 1 or 2+ reaction we will have several smaller clumps and a cloudy background. With mixed-field we will probably have just one larger clump and a cloudy background. For example, let's say a B patient received O units. When we forward type that patient's RBCs after the transfusions, the anti-B reagent will cause agglutination with the patient's own B cells resulting in the one clump. The transfused O cells will not agglutinate with the anti-B reagent and therefore will remain free causing a cloudy background.