You wouldn't know definitively without standards, but it's typical in HPLC methods that the first few minutes will be chemicals that passed through the HPLC column without absorbing to the column. Chances are they designed the HPLC method so that happened. The 'other ingredients' are usually called 'excipients' in the pharmaceutical industry, and they may be proprietary mixtures of stabilizers/preservatives/flavor additives/ etc...
hi everybody, i'm from Vietnam, my daughter want to research this HPLC video, but we can't catch the speed of the reader. Can anybody help me to give my daughter the subtitile to understand what this video talk about ? please help us ... many thanks
I use one of these machines everyday, and don't know much about them. (neither does any of the other employees tho) This was somewhat informative. I've been self learning chromatography ever since I started this job. Kinda hard to do tho, as I'm not a chemistry buff...
@282sanjay Yes you can use HPLC to measure these. However, I work in a hospital and we measure T3, T4 TSH and calcitonin via immunoassay. Its a much cheaper, faster and more sensitive method. You can obtain results within 30minutes, compared to HPLC which takes atleast a few hours and more working time to extract
The presentation was great, and the lab work too. But there were some glitches. The chart didn't show perfectly defined peaks for the strong substance. Then, the needle of autosampler was not shielded.
Very amazing video nice and simple , but why u didn't add the applications of the HPLC it would be of great important to those who use it in pharmaceutical industry ,,
the liquid phase is polar. h2o is a polar substance which means that things that are polar will more easily dissolve in it. that means that the more polar substances will have an easier time following the water. don't forget that in solutions like dissolves like.
exactly! Because the stationary phase is non-polar, the non-polar component had a greater affinity to it. In other words, it would spend more time being attracted to the stationary phase in comparison with the other component.
The quality of those peaks, tho... I do LCMS data review and you have no idea how happy I get when I see perfect chromatography. If the peak is messed up I have to give it a makeover with the software and possibly reinject the whole damn batch. Not a fun time.
Hi, This is likely because, as implied in the video at 3:41, the light needs to be set at a wavelength that is absorbed by all the components to be separated.
You tube University does it again... thank you
Very informative, simple and clear..specially for students..thanks for uploading
You wouldn't know definitively without standards, but it's typical in HPLC methods that the first few minutes will be chemicals that passed through the HPLC column without absorbing to the column. Chances are they designed the HPLC method so that happened. The 'other ingredients' are usually called 'excipients' in the pharmaceutical industry, and they may be proprietary mixtures of stabilizers/preservatives/flavor additives/ etc...
hi everybody, i'm from Vietnam, my daughter want to research this HPLC video, but we can't catch the speed of the reader. Can anybody help me to give my daughter the subtitile to understand what this video talk about ? please help us ... many thanks
It's a quite beneficial video. Thanks to all contributors.
I use one of these machines everyday, and don't know much about them. (neither does any of the other employees tho) This was somewhat informative. I've been self learning chromatography ever since I started this job. Kinda hard to do tho, as I'm not a chemistry buff...
Thank u so much for making this video I did not see hplc operates before this video
British people are good at explaining things.
@282sanjay
Yes you can use HPLC to measure these. However, I work in a hospital and we measure T3, T4 TSH and calcitonin via immunoassay. Its a much cheaper, faster and more sensitive method. You can obtain results within 30minutes, compared to HPLC which takes atleast a few hours and more working time to extract
thank you, is very academic for people that want learning HPLC
Yes, but only in aid of the separation. Then you would do 1H-NMR/13C-NMR or mass spect for identification.
cheers
@saurabhCherished I would suggest normal chromatographic columns, if the product is well known, an affinity column might be aqble to do the job.
The presentation was great, and the lab work too. But there were some glitches.
The chart didn't show perfectly defined peaks for the strong substance. Then, the needle of autosampler was not shielded.
Very simple explantion! Very helpful!
Very amazing video nice and simple , but why u didn't add the applications of the HPLC it would be of great important to those who use it in pharmaceutical industry ,,
brillant work,very informative and thanks
Acetonitrile is the primary NIST and IUPAC name, and I've never seen it bottled commercially other than Acetonitrile.
Yes, for quantitation or identification.
nice vidio this is very usefull for clear basics about hplc
@Yibitech
So which technology is used for large quantities?
The video appears to be quite useful... thanx for that
This is a brief but good lecture.
Very so helpful, thank you so much!
Can HPLC be used to extract the individual ingredients from the injected solutions?
Thx, helps me for my test in 2 days :)Greetings from germany!
Its a Jasco HPLC system, do a quick google search for "jasco HPLC" it should yeild some results
Brilliant video. Very clear.
Coooool can’t wait for tomm!
I need to determine residual sugars in the shake flask cultures. I need to prepare culture supernatant for analysis by HPLC. How can I do that?
What is the price of a unit like this?
can HPLC be used for finding the chemical content of fuel oils (bio-diesel)....?????
thnx helped a lot with my chem cswrk!
oh god you simply opened the column?!?! is it recommend??? first time I could see the powder of silica from the coulmn...
anybody know whre to buy this product?can gime me web site or company supply this product.thanks.
can anyone explain why the more polar component came out first? is it because the stationary phase is non- polar?
Ivan Torres you got it
the liquid phase is polar. h2o is a polar substance which means that things that are polar will more easily dissolve in it. that means that the more polar substances will have an easier time following the water. don't forget that in solutions like dissolves like.
Ivan Torres Yup.
Ivan Torres yeah and affinity too
exactly! Because the stationary phase is non-polar, the non-polar component had a greater affinity to it. In other words, it would spend more time being attracted to the stationary phase in comparison with the other component.
Thanks! this really helped. I wonder how they use this along with MS.
very good illustration !!
The quality of those peaks, tho... I do LCMS data review and you have no idea how happy I get when I see perfect chromatography.
If the peak is messed up I have to give it a makeover with the software and possibly reinject the whole damn batch. Not a fun time.
Is there any recommendation for effective HSCCC (high speed counter current chromatography) ?
hey really great video guys, keep up the good work ladzzzzzzzzzz
great for university students in the first and second grades
Thanks for posting! Very Informative!
what would a home set up cost?
Thank you for this video.
very useful for proteomics studies.
no i disagree all of what she said is wrong, she got her a and her bs mixed up
How to use in only q c
I have an essay ... hplc .. not sure how to start or structure it sooo much research out there 🙄
Great video! Thanks
Excellent, very informative
Very very good! Thanks....
ethanenitrile that's the correct way of saying it acetonitrile is old terminology although many books are still using it unfortunately
really a nice one for d beginners
This are very interesting importance instruments
This is what i do for work!
Gud 4 u
Nice. Do you work with the machine itself or do you do data review?
Jah Kah me too. But this equipment is very old...
great video that we want
its really great
Good explanation...
Thanks a lot ! very useful
very good !!
good video....................thanks
ethane nitrile... also known as acetonitrile. If it says ACN on the bottle, why not use the more common name :) good video though.
because British
Because those who know - know? To those who don’t know, it really doesn’t make much difference.
Thanks for the vid !
thnks a lot. its really helpfull
Thank you great video
Great video
Well explained tutorial, thank you.
Thanks for this video......good...
So nice
manual injection, dang that's an old instrument
***** 30 years ago, we used to have to pack our own columns!
for each run?
I'm more concerned with consistency between column to column. lol.
thank you. it was helpful
thank you very much.
thanks for uploading
amazing thank you
thanks for this video
awesome
10years ago 😯😶
nice video,
amazing
gooooooooooood and very nice
thanku..
thank U ! That will help me :)
Thanks ...
lifesaver
nice one
great
anyone can explain me why do we use UV spectra to get a peak in HPLC method. Even though there is other spectrum such as IR, X-ray,..etc. ?
Hi,
This is likely because, as implied in the video at 3:41, the light needs to be set at a wavelength that is absorbed by all the components to be separated.
Thanks good video
agreed, visuals very clear
cool video, makes more sense..i think!
nice video
bravo bravo score
super
Tomorrow we have exam in HPLC , chemical science Baghdad university by Dr.yahia 😍😂
good
Useful for a lvels
nice vedio
In other words, if you have to ask...
My Professor told me its about $30,000
biri bunu türkçeye çevirse muhteşem olur :)
70,000AU
Nice try ...i more study and more impressive poster prsentation...