Great in depth video about qPCR set up and data interpretation. Extremely useful for beginners and a great refresher for more experienced users. Lots of great practical tips and theoretical considerations. Dr. Scupham, you rock!
I am a biology graduate and working as an laboratory analyst for 1.5 years. For I only working with traditional microbiology testing procedures, it is so hard for me to understand how PCR works. This video helps me a lot as a total beginner in molecular biology. Thank you USDA and Dr. Alexandra.
Awesome video on qRT-PCR. Learnt alot for personal development. Thank you Dr Lexa Scupham for this educational video as a lab personnel. Thumbs to you and your team.
Thank you so much, Dr. Lexa Scupham, for this amazing video. It is clear and very informative. I took some notes to help me with my experiments and protocols. I can say that now I understand how real-time PCR is performed.
Thank you very much for sharing your insights and knowledge filled videos !! Professional and informative !! Outstanding !! Greetings from California … I wish you and folks good health , success and happiness !! Much Love ✌️😎💕
you did a great effort Dr. I must say that it is one of the best tutorial with practical information and details on real-time PCR. thankyou so much. you rocked. Dr. Sumaira
This video is so informative; I have learned a lot. Thank you for putting this together ❤ In the preparation of the standard, did you add 5 micro liters from the 10:1 tube to the 10:0 tube? I am a bit lost there.
sometimes when i take like 0.5ul dna or 12ul mix there will be always leftover liquid in the tip that doesnt want to come out. is it because my micropipette is not good anymore or it just the way i use it is not proper?
Dear Madam, pls accept my gratitudes. you are an an gel for me.I am struggling with qPCR and did not know how to start! can you please tell me the paper work you did for your lab pcr, how can I set mine for my lab?
Hi, madam I watched this video and really understand your explanation. Please do a favor, I need video like this for agrobacterium infiltration in tobacco or etc. Thank you
Wow, this is a great video. I’m not only talking about qPCR but in general lab work. Also amazing and clear explanations and suggestions. It was really interesting and I actually enjoyed it a lot. Thank you so much!!!
can anyone help mee pleasee... i want to separate mRNA(bax gene) from rat brain cells...and later wana do rt pcr to check bax expression level.. how will i know that the RNA which i separated is BAX...because there are many other mRNAs present in that cell.. please clear my confusion
Hi ; thank you for the explanation; how can we contact you if I have some queries; can you give your email? How to validate the process and what are the validation parameters for PCR
Great Job!, thanks. I wonder where to get/prepare the starting materials for the standard curve. How to know the number of copies of my plasmid in the sample. Is this a linear DNA plasmid or RNA transcript?
I have never seen such an informative and comprehensive video on qRT-PCR. Thank you so much, ma'am.
18:20
Not to be dramatic but this video changed my life. Thank you so much.
Great in depth video about qPCR set up and data interpretation. Extremely useful for beginners and a great refresher for more experienced users. Lots of great practical tips and theoretical considerations. Dr. Scupham, you rock!
I am a biology graduate and working as an laboratory analyst for 1.5 years. For I only working with traditional microbiology testing procedures, it is so hard for me to understand how PCR works. This video helps me a lot as a total beginner in molecular biology. Thank you USDA and Dr. Alexandra.
Awesome video on qRT-PCR. Learnt alot for personal development. Thank you Dr Lexa Scupham for this educational video as a lab personnel. Thumbs to you and your team.
Actually I have never found such an expertised content anywhere! I really do appreciate the quality of both information and quality of images!
Thank you very much for this detailed and comprehensive video, Ma'am. Please keep creating and sharing more such videos.
Very detail and informative , thanks a lot.
OMG .... what an excellent video
this is by far the best PCR demonstration I've seen. Thank you!
One of the best videos I have seen to date!
Wonderful thanks for your great work
The most comprehensive video on RT-PCR I have ever seen. Thank you very much Dr. Lexa Scupham 🙏
Thank you so much, Dr. Lexa Scupham, for this amazing video. It is clear and very informative. I took some notes to help me with my experiments and protocols. I can say that now I understand how real-time PCR is performed.
you really nailed it amazing each and everything very well explained it helped me in my research a lot
Thank you very much for sharing your insights and knowledge filled videos !! Professional and informative !! Outstanding !!
Greetings from California … I wish you and folks good health , success and happiness !! Much Love ✌️😎💕
Great demonstration to teach newcomers to PCR protocol
I Am A LAB Techinital and I werked For 3 yers and am learnig adegree program so thank you for this pcr system used you continu I like you Dr Alexandra
Great and in depth video about qPCR. It is really helpful and informative to beginners like me. Thank you Dr. Scupham.
very wonderful teacher, so fulfil and hope for more of this
very good and thorough video. Better than some undergrad labs. Thank you
Thank you Dr. Scupham for this wonderful video.
Thank you Prof!!
Really Appreciating
Great simple and straight to the point!
THE BEST TUTORIAL I HAVE EVER WATCHED. YOU ARE GREAT
Thanks for this wonderful tutorial!
This video is so informative; I have learned a lot. Thank you for putting this together ❤
you did a great effort Dr. I must say that it is one of the best tutorial with practical information and details on real-time PCR. thankyou so much. you rocked. Dr. Sumaira
Detailed step by step guide on qPCR analysis, great video, thank you so much for sharing!
extremely important video and an excellent teacher. Thank you
Good job Hannah and Dr. Lexa!
This video is so informative; I have learned a lot. Thank you for putting this together ❤
In the preparation of the standard, did you add 5 micro liters from the 10:1 tube to the 10:0 tube? I am a bit lost there.
It's been so informative and thanks for doing this video Dr.
well done, it was fantastic, I love the way you mentioned the details and cautions, truly thanks for that
Indeed helpful
it was greattt thank youuuu
I wish I could like this video a million times,Dr you did a massive job.Thank you😍
This is a really informative video! Well done.... Thank you very much!
Wow. Amazing
thank you Dr. Lexa the demonstration is simple for beginners like me, I quite enjoy the video
Is there a similar video preforming a gc-ms ?
Thank you very much for this great video. Love from Tunisia 🙏
Great effort dr. Lexa, highly appreciated your efforts in this regard.
This video is very informative. Thank you so much for Explaining the PCR. I had no idea what PCR was! Your video is very helpful!!!
Thank you for the video. But why didn`t you use gloves at the beginning?
This is a great full overview of qPCR. Thank you, Lexa.
wow i love this contenent. !!!
Thanks very good explanation along with the work
The lab safety/good manners tips were terrific!
Thank you Dr. Lexa. I enjoyed your video, its clear and informative . How about nested PCR?
sometimes when i take like 0.5ul dna or 12ul mix there will be always leftover liquid in the tip that doesnt want to come out. is it because my micropipette is not good anymore or it just the way i use it is not proper?
This was helpful and very informative. thumb's up
It was really greate.. But can u provide the softcopy of the information sheet you used in the video.
Thank you. You are life saver. 🙏
Thanks for your sharing.It's very helpful
Thank you so much. Indeed, very informative.
Do you have any spots for hiring?
Dear Madam, pls accept my gratitudes. you are an an gel for me.I am struggling with qPCR and did not know how to start! can you please tell me the paper work you did for your lab pcr, how can I set mine for my lab?
Can someone explain the dilution series she did I am lost by the math on those for this video.
Hi, madam I watched this video and really understand your explanation. Please do a favor, I need video like this for agrobacterium infiltration in tobacco or etc. Thank you
Wow, this is a great video. I’m not only talking about qPCR but in general lab work. Also amazing and clear explanations and suggestions. It was really interesting and I actually enjoyed it a lot. Thank you so much!!!
can anyone help mee pleasee...
i want to separate mRNA(bax gene) from rat brain cells...and later wana do rt pcr to check bax expression level..
how will i know that the RNA which i separated is BAX...because there are many other mRNAs present in that cell..
please clear my confusion
pls send some some sheet if possible because sheet shown in this video are not properly visible.
thanking you.
Hello, could you please give us the information in the technical shit the paper contening all information about hte volume of each component thanks
Well done description fully understood
Great. Learned a lot.
Nice video but can't you use the autoclave machine to sterilize the tools
Thank you sooo much mam for beautiful detailed explanation.😃😇😊
Dude this is such a good video holy shit
What is the differences between qPCR and RT PCR?
Is it absolutely necessary to linearise plasmids before RTPCR?
Yes , I think so.
Really Easy and very Realistic.
me gusto mucho
Best video on TH-cam.
Thank you so much madam for this detailed and in depth video.
Hi ; thank you for the explanation; how can we contact you if I have some queries; can you give your email?
How to validate the process and what are the validation parameters for PCR
Thank you so much!
Great video!😆
Thankyou .Madam..it was a great real time experience with your beautifully explaination in simple way..
Dear madam nice over view on Real time PCR. The primers used in real time PCR can be used in normal PCR without probe
Great job! ma'am thanks
Great Job!, thanks. I wonder where to get/prepare the starting materials for the standard curve. How to know the number of copies of my plasmid in the sample. Is this a linear DNA plasmid or RNA transcript?
best video ever seen
Thanks Ma'am 🙏😊
这就是专业
Many thanks to you, doctor.
I wonder if you generously explain how I can get the " working paper" 👍🌹
excellent explanation madam
Great videoo maaam.
💯
Thankyou Very much!
thanks for explain maam
Good explanation , keep it up ,
Plzzz told about optimisation and cDNa dilution also
I watch Twiv which is a virology podcast and they mention the use of PCR for testing of all kinds of viruses including SARS.
Pls send this sheet not visible
Mam can you share soft copy of it to us???
vortexing for a minute lol
why no gloves?
She did not glove up during instrumentation however she did during the process
Can I have a copy of your datasheet in this video to use as a template for my experiment -- to model another datasheet on? Just asking.
could anyone share the chart shown in 08：00 ？