HPLC | High performance liquid chromatography
ฝัง
- เผยแพร่เมื่อ 16 มิ.ย. 2024
- HPLC is also known as high performance liquid chromatography or high pressure liquid chromatography. HPLC is usually a modified column chromatography. In HPLC, the a high pressure pump is attached with with the column. This high pressure pump can generate pressure upto 40Mp. The column is further filled with the adsorbent material that has very small particle size.
The small particle size gives a very large surface area for the sample molecules to interact. As a result the efficiency of separation increases giving high resolution.
Following are the different components of HPLC.
The column
The column is made up of stainless steel and can withstand very high pressure upto 50MP. The length of column can vary from 5 to 25cm and has an internal diameter of 4.5mm.
The stationary phase
The stationary phase is made uo of an adsorbent material that has very small particle size. The particle size is kept uniform to obtain better performance.
The mobile phase
Mixture of different solvents is used as a mobile phase. The solvents used depends on the type of sample molecules to be separated. The mobile phase solvents can be polar or non polar.
The detector
In order to detect the eluite (ie sample coming out of the column) the HPLC column is attached with the detector.
Here cuz I lied on my resume
How’d it go?
@@haven3911
It actually went really well. It’s not that I have no idea what an hplc is but I was not proficient enough to put it down in my resume by any means. I didn’t get that particular job but I applied to a few other and within a couple of weeks I became an analytical chemist and I am very well proficient in it now :)
Hey can you help me
youtube once again helping more than the university instructors
TH-cam is ❤️
Yes
Your absolutely 💯 correct
Exactly!!
O yeah
Well, as a guy who daily works with HPLC have to say video is very good for brief introduction. Obviously HPLC is more complicated, with much more details. Just want to give shout out to all chemistry students and tell them to not be afraid of HPLC. In most cases, it is easy to develope a method on HPLC to determine some kind of compound, it might take just a few tries to make sure your compound of interest is separated from rest. Just always remember to filter your samples using 0.45 um filters and inject clean samples, also use guard columns :)
Dear, It is grateful when I read your comment. Could you suggest some videos which relate to developing method analysis on HPLC. I am a student who is learning HPLC to support for Master's and phD courses. Therefore, I appreciate if you could support me. Thank you so much.
don t forget to mesure you r standards corectly and shake them on ultrasonyc device It happends to my colegue and he finds out after 3 days XDDDD but I have a question for you if you work on hplc are you working on spectofotometers that use uv light?
@@animaluveteranu9523 UV/VIS thermo Dionex Ultimte
Hi there, your latest post about hplc really caught our attention. We are the manufacturer of hplc and have 20 years experience, if you are interested, provide an email address and we can send you the case study proposal.
hey, uhh.... can you please tell me if he said absorbent or adsorbent material? I mean when he is talking about the material in the column...
1:34 Edit: Nevermind, it's adsorbent material
Awesome explanation. No need to search for any other topic. Its a complete combo package.
Thank you very much for making such a video.....
Real-life heroes don't wear capes, a man once said.
Thanks a lot!
thank you for this video! i'm in a biochem grad program but coincidentally never took an actual biochem in undergrad, so these videos are helping a lot!
Thanks!!
I am truly amazed by chemists and engineers that develop these analytical instruments
Wow, a really great explanation! If this video was launched during my college time six years ago, I would have been a great Chemist who confident about using HPLC.
Hi there, your latest post about hplc really caught our attention. We are the manufacturer of hplc and have 20 years experience, if you are interested, provide an email address and we can send you the case study proposal.
perfect explanation, thank you!
Great video and very simply explained.Thank you so much!
Explaining amazingly giving maximum concepts
Thank you so much sir it's really a clearcut explanation i can't believe this almost a huge explanation is covered in 7mins 36sec
This video is a life saver. Thank you very much . Importantly, I have subscribed to this channel.
described very well.Understood the content clearly.Thank you very much.Plz do more clear lessons.
The best explanation of HPLC. Thank you so much
love this channel. from bangladesh.
Currently working on my internship report and needed a quick refresher. This video has been one of the most time efficient summaries i've found.
Cheers!
Hi there, your latest post about hplc really caught our attention. We are the manufacturer of hplc and have 20 years experience, if you are interested, provide an email address and we can send you the case study proposal.
Excellent explanation i undertood very easily
that was awesome, thanks sir.
SO SIMPLE AND INFORMATIVE! THANK YOU
Great video as always, thanks so much!
Thank you so much..With in a short time you've explained all the basic things in a wonderful manner with the help of drawings.
My phone fell off and that's why I'm here, I've always been curious how the experiment I had in my physics class worked 🥰🥰 thanks for that
This TH-cam helps me more than my teacher in the college.
Absolutely ,This was helped me to undestand about HPLC.Thank you ❤.
Brief and clear explanation... Thank u❤️
Within 2 hrs I have my xam. It's a 💎 for last min preparation
.
..
very helpful, thank you for making the video. much appreciated
superb clearly understand
Back ground music is not needed ,,it disturb the understanding
You saved my life. Thanks man
Very well explained 👏
Thanks for this video
You're the real MVP.
Thank you so much. Very nicely explained with animation & way u speak. Plz keep it up.👍👍👍
Good video! Thanks for that :) Cheers from Germany
Bastante útil para abordagem preliminar sobre HPLC
tHANK YOU SO MUCH FOR THIS SHORT INFO!! SO USEFUL AND HELPFUL!BINGO!
Awesome really we are learning a lot freely. Thank you so much
this video helped me so much for a apresentation, thank you so much!!!
Thanks for video. It helped me to understand HPLC!!
Excellent video, thanks!
That is a very good explanation! Thank you, is there any paper that you suggest that explains the function of HLPC? In order to detect vitamin K with the technique of HLPC, is there only that procedure or is a combine procedure with another technique? Thanks
Lmao. I still did’t get it why my class need 8hour to explain HPLC instead of 10 min. Good work btw really appreciate your work ,very helpful for overview.
Love u & thanks ur making very good vedio ....I'm ur new subscriber from India biochemistry student
thank you for such excellent explanation
Simple and clear thank you 😊
I love your videos thank you so much!!!
That minecraft noteblock beat at the end tho, lit
Really really thank you for the video
The very best explanation
This was super helpful!
Best explained ever tysm♥️♥️♥️
Thanks, it was very clearly explained and specific. Neat diagrams and good presentation. Easy and not messy ❤
This was helpful. Thank you sir.
It's just awesome. Shorter video , better explanation.
Outstanding, Thank you ♥️
Amazingly explained!! 😍👍
Osmm it's enough for exam . 🔥
Awesome explanation,Thank you so much
What ye class explanation,,great video
Very well explained. I can now answer any question in HPLC with ease.thank you
O yeah
Very nice explanation. Thank you
Best video on HPLC
Thank you very much. nice explanation :)
Thank you for the vedio and by this vedio i understood the topic very clearly
Great Video ❤, Keep it up ..
Really awesome videos
explained very clearly and nicely
Thank you soo much for helping us
Love this Channel From Bangladesh
Great efforts thank u
당신은 한국의 한 고등학생에게 매우 큰 도움을 주었어요. 정말 감사합니다!!
The best lecture here
good explanation ..thanks
Fantastic video!
Very good, thanks a lot.
Very helpful review!
Thanks for the explanation
Very nice explanation...thank you
your video is excellent. can you tell us how you integrate HPLC- ICP
Nice xplanation
Best vedio to understand hplc
Very well described.
Thanks. You saved me
Thank you!
Amazing video THANK YOYUUUU
Easy to understand 👍🏻
Loved it!
Thank you very much . This video was very useful for my exam❤️
This was just randomly recommended to me. I don't even know what this is.
Lol🤣🤣🤣
I wish this was recommended to me earlier ...😂
Thankkkkk you so so much
Tq so much sir it's easy to exam preparation😇
here with a typical night of due paper in post-grad after not knowing anything about chemistry for the last 3 years
reconsider your chosen field maybe.....
Haha
Great video
Thank you so muchhhhhh!
God video, drenge!
Superb!!! 🙌
Awesome
Well explained 🧡
oh wow its very understanding
It was very much helpful 🤩🥳🥳