Fantastic stuff. I really liked the end where you suggested that leaves of different colo(u)rs are worth looking at and also about the possible relationship between levels of chlorophyll and growth rates. To be honest, with a little bit of variety, this simple qualitative experiment could be easily modified into a detailed experiment covering the 90 minutes that I have to do chromatography with some visiting junior high school students (here in Japan). Thanks for the great video and ideas.
IB students that missed an ISC class here are the answers - What are the pigments and why do plants and algae need them? The pigment of the plant or algae shows which colors they will absorb from electromagnetic radiation and the colors they will reflect - Explain the materials needed to conduct the experiment - Piece of coffee filter - 15ml of 95% ethyl-alcohol - A pinch of sand - 2 toothpicks - A mortar and pestle - 50 ml graduated cylinder - 250ml beaker - Test tube and rack - Plastic wrap - Ruler - Pencil - Scissors - 5-10 fresh spinach leaves - Briefly explain the method of the investigation - Cut the leaves into piece with scissors - Grind the leaves with the pestle for a minute - Add a pinch of sand while grinding - Add 10 ml of the ethyl-alcohol - Mix for another minute - Place the mixture in the 250ml beaker - Cover the top with plastic wrap - Leave for 10 minutes - Cut a strip of the coffee filter and make sure it’s longer than the test tubes - Roll an end of the paper around the tooth pick - Place the toothpick with the paper wrapped around in the test tube, with the toothpick resting on top - The paper should not touch the bottom - Take the paper out and measure 1-2 cm of the bottom end of the paper and draw a line across - Take the other toothpick and dip it into the mixture and place the sample onto the line previously drawn - Pour 5 ml of ethyl-alcohol into the test tube - Place the filter paper with toothpick back into the test tube and cover the whole thing with plastic wrap - Leave for 20 minutes - Remove the paper and let it dry GL bros
Ditto oh I thought I cared lol, you really have done an excellent job. Keep it up and kiss Bill Nye good bye. You do this EXACTLY the way science is supposed to be done. Just outstanding. Effortless attention to detail. I love it.
Total Chlorophyll: 20.2(A645) + 8.02(A663) Chlorophyll a: 12.7(A663) - 2.69(A645) Chlorophyll b: 22.9(A645) - 4.68(A663) some one please tell me what are these values 12.7, 2.69, 22.9, 4.68
Why a plastic wrap? Is it so that the solution is not exposed to air? But whats gonna happen if it is? Will it not work then? Im asking this because its really hard to find a plastic wrap like that one where I live.
I would say the ethyl alcohol, which has a boiling point of ~78 °C, would evaporate and leave the exposed test tube. Ethyl alcohol is flammable and poisonous with skin contact or ingestion in its pure forms. So if you plan to do this, do it under caution.
@@morganm1931 ok thanks. I'm trying to make a bright green sprayable liquid varnish/glue that will slowly lose its bright green colour (chlorophyll) Do you have any tips?
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Reminds me of those 90's science videos you see as an elementary student.
Fantastic stuff. I really liked the end where you suggested that leaves of different colo(u)rs are worth looking at and also about the possible relationship between levels of chlorophyll and growth rates. To be honest, with a little bit of variety, this simple qualitative experiment could be easily modified into a detailed experiment covering the 90 minutes that I have to do chromatography with some visiting junior high school students (here in Japan). Thanks for the great video and ideas.
IB students that missed an ISC class here are the answers
- What are the pigments and why do plants and algae need them?
The pigment of the plant or algae shows which colors they will absorb from electromagnetic radiation and the colors they will reflect
- Explain the materials needed to conduct the experiment
- Piece of coffee filter
- 15ml of 95% ethyl-alcohol
- A pinch of sand
- 2 toothpicks
- A mortar and pestle
- 50 ml graduated cylinder
- 250ml beaker
- Test tube and rack
- Plastic wrap
- Ruler
- Pencil
- Scissors
- 5-10 fresh spinach leaves
- Briefly explain the method of the investigation
- Cut the leaves into piece with scissors
- Grind the leaves with the pestle for a minute
- Add a pinch of sand while grinding
- Add 10 ml of the ethyl-alcohol
- Mix for another minute
- Place the mixture in the 250ml beaker
- Cover the top with plastic wrap
- Leave for 10 minutes
- Cut a strip of the coffee filter and make sure it’s longer than the test tubes
- Roll an end of the paper around the tooth pick
- Place the toothpick with the paper wrapped around in the test tube, with the toothpick resting on top
- The paper should not touch the bottom
- Take the paper out and measure 1-2 cm of the bottom end of the paper and draw a line across
- Take the other toothpick and dip it into the mixture and place the sample onto the line previously drawn
- Pour 5 ml of ethyl-alcohol into the test tube
- Place the filter paper with toothpick back into the test tube and cover the whole thing with plastic wrap
- Leave for 20 minutes
- Remove the paper and let it dry
GL bros
Excellent video.. and I really liked your personality..
Keep it up.
What a clear explanation!I enjoyed it .
Excellent video..It helped me a lot.And awesome explanations
Ditto oh I thought I cared lol, you really have done an excellent job. Keep it up and kiss Bill Nye good bye. You do this EXACTLY the way science is supposed to be done. Just outstanding. Effortless attention to detail. I love it.
This is great, I did exactly the same stuff in biology back in the early 2000s!
he's so adorable, thats besides the point lmao, this video actually helped me and got me more enthusiazed for chem
Awesome video thanks for explain us very clearly
Great video! Informative and made me smile. If you don’t mi no me asking, what is your hair and skin care routine? You look radiant
Mind blowing explanation bro .it helpful for my practical exam tq bro
this is my brother omg never knew he had appeared here
well you should’ve asked him
REALLY
UR BROTHER YEETED THAT PLANT ITS FUNNY AHAHHAHAHHAHAHHAHAHA
ARE YOU HIS SISTER OR BROTHER???
Great video. Thanks
disappointed to see him tossing that potted plant at 0:44 :(
he really do be ruining that plant’s life doe 😳😳😳
Excellent explanation!
The explanation is fun and clear
This is like the informative version of the 'this is how you cut kale stems' eHow video
Great sir
Thank you teacher
He‘s a lefty 😱😱
Your voice reminds me of LeVar Burton, and that's pretty cool! Good video too!
what are the leaves we should use (other than spinach) to see the results distinctly and clearly ? PLS HELP !
trees leafs for example
thank you.... :-)
My teacher recommends Kale, Lettuce, Spinach, Collard Greens, or Romaine Lettuce. But you should avoid using endives, iceberg lettuce, or leeks.
Total Chlorophyll: 20.2(A645) + 8.02(A663)
Chlorophyll a: 12.7(A663) - 2.69(A645)
Chlorophyll b: 22.9(A645) - 4.68(A663) some one please tell me what are these values 12.7, 2.69, 22.9, 4.68
ITS VERY INTERESTING
THANKU THANKU
Did you try the red or yellow leaves? =] love this stuff!
The explanations were very helpful!
Thank you!
NC
Why a plastic wrap? Is it so that the solution is not exposed to air? But whats gonna happen if it is? Will it not work then? Im asking this because its really hard to find a plastic wrap like that one where I live.
I would say the ethyl alcohol, which has a boiling point of ~78 °C, would evaporate and leave the exposed test tube. Ethyl alcohol is flammable and poisonous with skin contact or ingestion in its pure forms. So if you plan to do this, do it under caution.
Nick Biggelaar
super video it's clearly understanding
Can i use 80 proof ethanol to extract the Chlorophyll?
Yes but add more than you would with 95%
@@morganm1931 ok thanks. I'm trying to make a bright green sprayable liquid varnish/glue that will slowly lose its bright green colour (chlorophyll) Do you have any tips?
How i can estimate vitamin A from a tomato
Thanks! :)
I did not understand why alcohol is used and water can not be used
has twenty minutes really passed or was there just another test tube already done? :)
Can u please say what is a filter paper and where we can get it
You can use a coffee filter or cheesecloth.
Very nice so thanks
do you boil perroleum ether and propanone when added in test tube ????
would this work with other things, like a banana for example, to extract other types of pigment molecules??
+Ashleigh Braganza It will work with any type of leaf, but not with bananas! A banana is a fruit! JEEZ!!
Thank you
Really helping video
Nyc..
What is the name of the liquid you put in the morter? (when you add the sand)
95% Ethanol (Ethyl alcohol)
thanks!
What solution he has added to spinach????
Laraib Sultan: ether alcohol
95% Ethanol (Ethyl alcohol)
Rubbing alchohol
how can we calculate RF value from Ur chromatogram,?what u add to grinding spinach before alcohol,why?
Poornima Kommoju rf equals to distance moved by pigment from original spot divided by distance moved by solvent from original spot
thanks
thank you😊😊😊☺☺😃😃
Thanks 👍🏼
What a nice guy
Op video xd
U naughty naughty u teasing me u naughty naughty
what are the names of the other pigments
Xanthophyll and Carotene
weird choice of music tottally
What pigments did he find?
2013??? Bruh
nice
good
Virk explained it poorly😕
thnx
Who is watching in 2024
👇
Hindi me video banaye sir
I like it...
❤️❤️
i love you
Noice
fais toi pousser le bouc et la moustache je te jure tu serais plus beau ma vie
You should not throw a plant like that .
Bruh it's just to teach Interestingly
And besides the explanation was very clear
You are taking it more serious WTH
5 Е привет я Кайсар
Lol
pretty stinky
Bad pictures
cringy af
Not more than you:)
I don't like it i give dislike
It would be better if you keep your opinions to yourself:)
terrible
He's trying his best and it wasn't terrible
Stop being nagative:)