I promise you are a life saver!!!! I take Biology online and I'm not your typical science major. This video is a great breakdown of the process for my next lab. Thanks a bunch!!
A suggestion for keeping the filter paper from touching the side is to lay a pen or pencil across the top of the bowl/glass and then tape the filter paper perpendicularly down from there. It has worked well for me in the past with ink chromatography.
This video is amazing! I used Momordica charantia (bitter melon), Moringa oleifera (horseradish), and Psidium guajava (guava) and was astounded with the result of my experiment in paper chromatography. I watched your video and followed it closely and it helped me a lot throughout the process. With the help of your video, I witnessed how great science is when understood. I am still amazed at the partition where the solvent seeps along the paper carrying the substances from the mixture to the one end of the filter and the pigments were separated; the result was even impressive after it dried. Students like me are truly grateful for your amazing video. Thank you, Dr Dainis, I am looking forward to learning more about Science with you!
Chlorophyll is more polar than the carotenoids, so it sticks to the paper a bit more. This means the carotenoids are drawn up by the liquid faster and appear above the chlorophyll.
Alex, you have great enunciation. And just the right amount of movement, neither still as a statue nor spazzy. Knowing that your already good video skills will only get better earned you a subscription.
I have used the common leaves found near my house, it includes: Water Spinach (Kangkong), Oregano, and Malabar Spinach (Alugbatti). With the help of the video of yours, I followed the steps of this method, Chromatography, and I had observed that the molecules have different polarities interacted with the coffee filters differently, layer by layer.Thank you for sharing your knowledge, Dr. Dainis, I enjoyed doing it so!
This is because the isopropyl alcohol pulls the water out of the leaves through the porous membranes of the leaves by osmosis, right? And as the water is drawn out of the leaves it pulls the compounds with it, and the compounds like chlorophyll and carotenoids are drawn up the filter paper by diffusive capillary force, right?
If the leaf is colored, it has pigments and they can be separated. Now if the leaves are all brown the pigments have probably broken down already, but if you live in an area where the leaves don't change color, they still have pigments and can still be separated.
Strictly speaking this is not paper chromatography. The solution in the beaker is just the solvent, not the mobile phase of chromatography. The trick is to choose a mobile phase which separates the different components of the mixture.
I used oregano, chinese evergreens, and devils ivy. And the result was great the color of the isoprophyl inside the glass with some leaf really changed, and the paper changed color a bit but it was really amazing. Thanks for the info Doc. I've learned a lot.💙
You're right Alex. Xanthophylls are part of the carotenoid group. Leaves normally contain both xanthopylls and carotenes and as for which one dominates depends on the type of plant. Spinach leaves for example are famous for their high lutein (a xanthophyll) content because they have significantly more lutein than beta-carotene.
"the leaves are being seperated from alcohol phase, the mobile phase and Drawn up into the paper, which is the stationary phase." Is this where we get the name"stationary" for paper and paper supplies?
Yup, different trees will have different pigments in different amounts - this is what gives them each their unique color! For these kinds of pigments, I'm not sure about if you can influence them (my guess would be yes), but I know you can in many flowers. For example hydrangeas will produce more blue blooms if planted in acidic soil and pinker blooms if planted in more neutral soil.
Hah, it's definitely not - most Bostonians drop their R's and turn O's into long A's. Who knows where my crazy voice came from. And thanks for watching!
Idea for another video? Anthocyanins are not actually always red(I know you know), its colours depends on pH of surrounding. Another pretty experiments can be done with them. You can use chromatography to isolate them and then make solutions with different pH, few drops of anthocyanine solution and magic!
Yeah, we´ve made that experiment very recently. Tip for cooking enthusiasts : try to add some sodium hydroxide to your red cabbage next you are cooking(no, don´t actually do that...) What are you studying, biochemistry?mol. biology? Super-duper videos btw :) and sorry for eng, i´am trying...
I love epigenetics (It's what I want to study in grad school) but there's so much and I think it's so important that I want to make sure I do it right. I will definitely make a video (or two, or four...) on it, I just don't know when/how yet!
Hi, I'm from Indonesia, glad to see that you are full of enthusiasm in providing the knowledge you have, sis, may I ask, how to treat living plants so that their color changes from green to partially white, and permanently, what should I do, thank you sis for the time . GBU
I got here from the numberphiles video. I'm definitely going to subscribe. I love videos that teach you things. And plus, your smile is so very charming.
I have in fact been keeping up with your videos, and they have all been very interesting. Your presentation is awesome, and you pick some really good topics. I even go around and tell all of my friends about some of the stuff in your videos. You have so much interesting information.
Maybe you can talk about electrophoreisis since I think this is used for DNA (though I don't know how the DNA is died, colored I mean) On youtube search for "Emily's home gel electrophoresis experiment"
For some reason I started to think of Baphomet when you mentioned the story of Pan and his horn(s?). Look up a picture of Baphomet, I think it is closely related to what you talked about.
Great initiative, its nice to see someone who simplifies experiments so that kids can try these at home. It would be even better if you slowed down your talking a bit, its hard to follow with your hand movements and fast talking. If you had less liquid in the cup the separation would have been easier.
Awesome experiment instructional! Also more informative than nottinghamscience's "Why do leaves change colour?" from the other day. I'll try the experiment just as soon as there aren't 60mph winds outside.
I believe xanthophylls fall under the carotenoids. I didn't want to get into too many of the types of pigments (chlorophyll a and b, etc) but yes, there were probably xanthophylls in there!
Wow, I admire you!! I have a question I'd like to ask. I'm doing a science fair project based on this experiment so it's the beginning of January already. Luckily, I have got some leaves from before hand but I only have two colors of the same bush and another color from another bush. Should I just use the leaves from the same bush or use the extra leaves from the other bush too? And would you mind giving me any advice for my project? Thank you, and greetings from California! (PS I have watched this vid about 5 times)
I think it would be interesting to try all of the leaves! That way you could compare both the same bush at different times and a different kind of plant. The more kinds of leaves you use, the more interesting things you might find!
Great video! I remember we did chromatography with blue ink in school, the result was some colorful paintings in the paper :P I will try to do this at home :)
hi. i am a korean student. i was impressed by your experiment. i want to capture this video and use it in science club. and i want to put the course in the club cafe by using this picture. i will reveal the source. can i use your video?
Oh man, I gotta remember chemistry now...Hmmm... Isopropyl alcohol and ethanol (like tequila) have different chemical structures. Ethanol is two carbons with an -OH (alcohol) group, isopropyl is three carbons with the -OH stuck to the middle one. If I recall correctly, ethanol is more polar (which will affect the separation), and tequila's only about 40% ethanol, so it might be too dilute, BUT I'd wager it will still work. You're just going to have to try it and find out!
Craaaazy idea, would the trees turn their leaves red before they fall off to help them decompose maybe? Then the tree can re-absorb them or what ever? Tree cannibalism!
I promise you are a life saver!!!! I take Biology online and I'm not your typical science major. This video is a great breakdown of the process for my next lab. Thanks a bunch!!
+Amber Walker So glad I could help :)
or should u say leaf saver..
A suggestion for keeping the filter paper from touching the side is to lay a pen or pencil across the top of the bowl/glass and then tape the filter paper perpendicularly down from there. It has worked well for me in the past with ink chromatography.
you're wrong you idiot
This video is amazing!
I used Momordica charantia (bitter melon), Moringa oleifera (horseradish), and Psidium guajava (guava) and was astounded with the result of my experiment in paper chromatography. I watched your video and followed it closely and it helped me a lot throughout the process. With the help of your video, I witnessed how great science is when understood. I am still amazed at the partition where the solvent seeps along the paper carrying the substances from the mixture to the one end of the filter and the pigments were separated; the result was even impressive after it dried.
Students like me are truly grateful for your amazing video. Thank you, Dr Dainis, I am looking forward to learning more about Science with you!
Chlorophyll is more polar than the carotenoids, so it sticks to the paper a bit more. This means the carotenoids are drawn up by the liquid faster and appear above the chlorophyll.
Who is watching this for science class?
David Peters I am also watching for my science class
wow cool man
David Peters meh ....😊😁
you suck.
big shlong.
Alex, you have great enunciation. And just the right amount of movement, neither still as a statue nor spazzy. Knowing that your already good video skills will only get better earned you a subscription.
I have used the common leaves found near my house, it includes: Water Spinach (Kangkong), Oregano, and Malabar Spinach (Alugbatti). With the help of the video of yours, I followed the steps of this method, Chromatography, and I had observed that the molecules have different polarities interacted with the coffee filters differently, layer by layer.Thank you for sharing your knowledge, Dr. Dainis, I enjoyed doing it so!
This is because the isopropyl alcohol pulls the water out of the leaves through the porous membranes of the leaves by osmosis, right? And as the water is drawn out of the leaves it pulls the compounds with it, and the compounds like chlorophyll and carotenoids are drawn up the filter paper by diffusive capillary force, right?
Target alcohol! Up and Up products go on sale across the board during the fall months.
If the leaf is colored, it has pigments and they can be separated. Now if the leaves are all brown the pigments have probably broken down already, but if you live in an area where the leaves don't change color, they still have pigments and can still be separated.
Omg.. That was magic, one minute she didn't have her glasses on and the next minute the glasses reappeared, I'm impressed..
Strictly speaking this is not paper chromatography. The solution in the beaker is just the solvent, not the mobile phase of chromatography. The trick is to choose a mobile phase which separates the different components of the mixture.
I used Oregano, parsley, and mint. As I observed it separate substances based on their color as was the case with herbal pigments.
what can I use instead of isopropyl alcohol ?
OMG you saved my life, without this video my project would have been a straight 0.
I used oregano, chinese evergreens, and devils ivy. And the result was great the color of the isoprophyl inside the glass with some leaf really changed, and the paper changed color a bit but it was really amazing. Thanks for the info Doc. I've learned a lot.💙
You're right Alex. Xanthophylls are part of the carotenoid group. Leaves normally contain both xanthopylls and carotenes and as for which one dominates depends on the type of plant. Spinach leaves for example are famous for their high lutein (a xanthophyll) content because they have significantly more lutein than beta-carotene.
Why can’t the filter paper touch the glass?
"the leaves are being seperated from alcohol phase, the mobile phase and Drawn up into the paper, which is the stationary phase."
Is this where we get the name"stationary" for paper and paper supplies?
Yup, different trees will have different pigments in different amounts - this is what gives them each their unique color! For these kinds of pigments, I'm not sure about if you can influence them (my guess would be yes), but I know you can in many flowers. For example hydrangeas will produce more blue blooms if planted in acidic soil and pinker blooms if planted in more neutral soil.
You are awesome. Good to see that I'm not the only dork who finds all this stuff fascinating. Subscribed.
we are starting spring, so we may not get many different results from the leaves
Can we use normal alcohol instead of this
Thanks! I was so excited to be in that video with so many awesome, amazing TH-camrs! Hope you stick around and learn something :)
is this a photosynthesis expiriment?
It's actually just "I Love You" in American Sign Language. Just trying to spread some love!
Thanks! I keep trying to make each one better than the last, so I hope you continue to enjoy them!
I don't mind losing to science. But yes I find your videos awesome. Can't wait to watch future ones.
Some leaves will actually turn purple to nearly black in the fall because the anthocyanins are produced before the chlorophyll degrades
Hello,What is the advantages of doing this?Because i need to answer with the asker when i do it.
Yeah, it gets a little echoey at times... I'll work on it!
OOO LOZER
Hah, it's definitely not - most Bostonians drop their R's and turn O's into long A's. Who knows where my crazy voice came from. And thanks for watching!
Idea for another video? Anthocyanins are not actually always red(I know you know), its colours depends on pH of surrounding. Another pretty experiments can be done with them. You can use chromatography to isolate them and then make solutions with different pH, few drops of anthocyanine solution and magic!
Do you know what we are measuring in the project
i wanna see what happens if i use blood to water my plants and then do a chromatography on em.
they would probably die right?
why do you use alcohol instead of water? Please answer this my lab is due tomorrow LOL
I'm stoked that NurdRage sent me in the direction of this channel. I love me some science videos!
Hey! New subscriber, medicinal chemist from Australia. This is probably getting old to you now but you are amazing! Keep it up!
Yeah, we´ve made that experiment very recently. Tip for cooking enthusiasts : try to add some sodium hydroxide to your red cabbage next you are cooking(no, don´t actually do that...) What are you studying, biochemistry?mol. biology? Super-duper videos btw :) and sorry for eng, i´am trying...
What type of leaves did you use?
what is the purpose of this experiment?
great video!!!! i wonder what will happened if i change isopropyl alcohol with hand sanitizer.
Where you live
I love epigenetics (It's what I want to study in grad school) but there's so much and I think it's so important that I want to make sure I do it right. I will definitely make a video (or two, or four...) on it, I just don't know when/how yet!
and also can any alchole be used for this experiment or is it only the type you stated?
Question: I'm doing this for a science fair project but how am I supposed to arrange my data?
Is there a way to the separate the alcohol and the chlorophyll so it would leave a powder or paste?
how much alcohol did you use?
@Alex Dainis Did it blind her?
I owe him a bag full of nickels, I feel so honored to be in that video with so many cool people! I'm glad you like it!
but what if im doing on how they get old
i want to do that in grad school too! What's a good one for it? I'm looking at MIT but I might need to look elsewhere.
Your are a life saver for my science assignment 😊😊
Hi, I'm from Indonesia, glad to see that you are full of enthusiasm in providing the knowledge you have, sis, may I ask, how to treat living plants so that their color changes from green to partially white, and permanently, what should I do, thank you sis for the time . GBU
I got here from the numberphiles video. I'm definitely going to subscribe. I love videos that teach you things. And plus, your smile is so very charming.
OMG THIS IS SUCH A LIFESAVER AND VERY EASY TO UNDERSTAND THANKS!
I have in fact been keeping up with your videos, and they have all been very interesting. Your presentation is awesome, and you pick some really good topics. I even go around and tell all of my friends about some of the stuff in your videos. You have so much interesting information.
I cannot believe how much content you got into 4 minutes and 24 seconds. Does the mass of a video increase if you put more content into it?
Very nice experiment of science l know many things about the leaves
the video image is too poor, you need to fix it more
Xanthophylls?
@Michael Lewis You are far from alone i Taught myself how to Program at age 13
Can you do a Bit Scized on the effects of alcohol on the body? And how exactly does alcohol poisoning work?
Maybe you can talk about electrophoreisis since I think this is used for DNA (though I don't know how the DNA is died, colored I mean)
On youtube search for "Emily's home gel electrophoresis experiment"
For some reason I started to think of Baphomet when you mentioned the story of Pan and his horn(s?). Look up a picture of Baphomet, I think it is closely related to what you talked about.
Most cooking/chef stores will have a mortar/pestle
.
i did it in shoo with some norma leaves and there were green, yellow and purple
Did you mean shoe or "schoo" ?
I use oregano , makahiya and lagundi leaf in this experiment 🥰
Great initiative, its nice to see someone who simplifies experiments so that kids can try these at home. It would be even better if you slowed down your talking a bit, its hard to follow with your hand movements and fast talking. If you had less liquid in the cup the separation would have been easier.
I did that with weed once, but I was looking to isolate different compounds.
Awesome experiment instructional! Also more informative than nottinghamscience's "Why do leaves change colour?" from the other day. I'll try the experiment just as soon as there aren't 60mph winds outside.
They certainly do, but I just thought that they were so beautiful that they should not go without honorable mention.
I believe xanthophylls fall under the carotenoids. I didn't want to get into too many of the types of pigments (chlorophyll a and b, etc) but yes, there were probably xanthophylls in there!
Good little teacher, like that style of course
Wow, I admire you!! I have a question I'd like to ask. I'm doing a science fair project based on this experiment so it's the beginning of January already. Luckily, I have got some leaves from before hand but I only have two colors of the same bush and another color from another bush. Should I just use the leaves from the same bush or use the extra leaves from the other bush too? And would you mind giving me any advice for my project? Thank you, and greetings from California! (PS I have watched this vid about 5 times)
I think it would be interesting to try all of the leaves! That way you could compare both the same bush at different times and a different kind of plant. The more kinds of leaves you use, the more interesting things you might find!
Thanks for showing us this channel.
Great video! I remember we did chromatography with blue ink in school, the result was some colorful paintings in the paper :P I will try to do this at home :)
hi. i am a korean student. i was impressed by your experiment. i want to capture this video and use it in science club. and i want to put the course in the club cafe by using this picture. i will reveal the source. can i use your video?
Where to get filter paper
idk
@@Geneboie Just use a plain coffee filter and cut it into stripes like in the video.
@@GRosa yea idid that yesterday, thanks for the tip even though I did it already, I appreciate you!
Gosh I started watching UR vids at 9 and I looked at the clock and know its 10.
why doesnt it work with water
Water isn't an efficient solvent.
Made on my B day!!! I love how you explain all this science in a fun way!
Awesome! Make sure you leave a video response of your experiment, or post photos on the facebook page - I want to see what everyone gets!
Nicely done! Would you mind telling me what video editor you use to create your science videos?
i just want to search how to make a leave from natural to artificial without changing its color
OMGGGGGGG this was so good my class liked it
1:42 that is one awesome theory, I love it :D
Thank you so much!!! But I have a question, It can be any kind of leave????
Of course!
if i do this experiment, which questions can (Maybe) they ask me? :)
Oh man, I gotta remember chemistry now...Hmmm... Isopropyl alcohol and ethanol (like tequila) have different chemical structures. Ethanol is two carbons with an -OH (alcohol) group, isopropyl is three carbons with the -OH stuck to the middle one. If I recall correctly, ethanol is more polar (which will affect the separation), and tequila's only about 40% ethanol, so it might be too dilute, BUT I'd wager it will still work. You're just going to have to try it and find out!
I have to do some assignments from home ( because of Corona ) you are a lifesaver!!
I kinda want a mint julep now
It would be very cool to see how the different seasons affect the colors from the same tree!
Your kitchen setup was borrowed in v=ej2zXOwASVI.
Huh. And they put their own watermark on top of it. How annoying... (But thanks for the heads up!)
That is a good point.
Yes! I've seen a pH indicator made out of cabbage before using this idea: super cool.
Hi I found this link from NurdRage, Like what you are doing here.
P.S.
I like the glasses quick change/continuity thing.
Nice, that's a really cool experiment!
Your accent is so unique!
Craaaazy idea, would the trees turn their leaves red before they fall off to help them decompose maybe? Then the tree can re-absorb them or what ever? Tree cannibalism!
I think the sugars would help feed the mycelium in the soil. So, yes. Your idea sounds feasible to me (I'm just someone from the internet, tho.)