Plant Pigments, Chromatography
ฝัง
- เผยแพร่เมื่อ 14 มิ.ย. 2016
- Using Chromatography to Investigate the Pigments Isolated from Leaves of Different Plants. Required A level Biology practical activity. Make sure you see the end where calculation of Rf values is explained. Method could be used to compare pigments in different plants such as shade loving and sunlight loving species.
![เบิ้ล ปทุมราช - อ้ายกินดอง น้องลาบงัว ft. สแน็ก อัจฉรีย์ [OFFICIAL MV]](http://i.ytimg.com/vi/-OK--KaSPcI/mqdefault.jpg)
“Stop Richard”
A small correction here though, at 8:15, the RF value must be smaller than one, not zero 👍
Thanks. I'm using this during the COVID19 quarantine for my class
Lindsey Mao Me too. Glad it’s useful.
Please make some more videos on the experiments of A2. Your videos are really helpful
Thank you so much.... Really saved me big time
Thank you I did a test with a 6 mark question about this experiment they did in class but i was out of lesson so got not enough marks and now resitting test
Ohh.. thank you so much 👏👏👏👏
Very interesting thank you
Thank you.
omg I found this so late
Thenk you
When grinding the leaf and sand and acetone...what is the liquid called that is produced?
Thanks thanks a lot because of you I learn this experiment tomorrow is my intermediate practical exam
you've saved my alevel thank you so much
Yes u r absolutely true.
Does the solvent have a collective name?
thank you too much mam
Thank you, it is a very informative video indeed. But is there really no substitute for TLC paper that can be used?
Instead of alumina plate, one can use silica gel plate that can be made from silica gel added with solvent and poured on a glass plate, leveled and kept in oven.. silica gel plate ready to use now
8:15 Smh "A value of less than zero"
Lol
What is the concentration of the acetone?
Nice work
so you don't need to strip the solvent from the pigments?
Is there any way to improve this method
What is the purpose of using sand?
Hanna Bouftah it’s gritty texture just helps to break up the lead tissue.
Biology Practicals and Revision Biology Tutor thank you
why did you add acetone during the extraction ?
Nurarihyon hafiz it dissolves the phospholipid membrane so that more pigment is released and acts as a solvent for the pigment.
@@biologypracticalsandrevisi8066 Ohhh I see..
Thank you
Does anyone know why you use a mixture of solvents instead of just propanone
The different pigments have different solubilities in the various components of the solvent. The mixture gives better separation.
@@biologypracticalsandrevisi8066
Thank you very much! :)
what does the propanone do?
Spoken word Spoken from the heart it’s is a good solvent for some of the compounds in the leaf.
Can you please say the exact pigment distance and solvent distances?
Sorry I can’t help with that. It was a while ago and I don’t have the raw results. The distances will change depending on the type of stationary phase and exact composition of solvent used.
Can u use water to separate the colors 🤔 I'm doing a PD lab for the first time
kaylia walker You could try it. You are unlikely to get a good separation but you should get something.
@@biologypracticalsandrevisi8066 but so I think I should try using the distilled water and the alcohol
@@africanqueen9820 Hi biology student here. From what I know, you can't use distilled water or even water to separate the pigments since the pigments are located in the thylakoid membrane inside the chloroplast, which means we are dealing with a membrane and membranes usually have a lipid constitution and if you want to 'break' the membrane to dissolve the pigments, you absolutely need an organic solvent, which is why you use acetone and petroleum ether. Please correct me if I am wrong.
@@martajackson11 thank you very much
where did you get the rf values from?
Theo You calculate it and compare to standard values for pure pigments.
@@biologypracticalsandrevisi8066 Sorry for the misunderstanding! I meant the source of the pre-determined Rf values that you compare your results to!
Theo there are a few txt books with them in or you can run pure pigments yourself with the same solvent .ill see if I can send you a link to them.
@@biologypracticalsandrevisi8066 Thanks!
Why would you use a sand and plant to grind the mixture
The rough texture of the sand helps to break apart the plant tissue.
@@biologypracticalsandrevisi8066 thanks
Mr kirsten man allow it
It would be helpful if you explained WHY you're doing what you're doing and not just describe it
Rachel Munday to find the rf value
"Stop Richard"
8:29
Why must it be a pencil line and not a pen line
Jasmine Wallace-Thompson if you use pen, the ink will dissolve in the solvent and run up the paper obscuring the results.
Biology Practicals and Revision Biology Tutor thank you so much! Another question why must the pigment spot be above the solvent surface?
Jasmine Wallace-Thompson if it wasn’t it would dissolve in the solvent rather than being carried up the paper by the solvent.
guzzo è un sottosviluppato
Ma cosa 😂😂😂
@@auroradeluca3898 ciao a tutti figaaaaaaaaaaaaaa
@@saratarantino3409 stiamo invadendo pure you tube
@@auroradeluca3898 infatti ;)
stop it char.......
Stop zooming already