Paper Chromatography Experiment
ฝัง
- เผยแพร่เมื่อ 29 ธ.ค. 2019
- This is an experiment to separate the various pigments found in green plants, using a popular and simple technique called Paper Chromatography.
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This really helpful for me before start self study of the lesson.. Thanxs so much..
Clear and concise! Thanks for making this :)
Thank you for this video! Is very helpfull! I have two questions, what plant did you use? I'm reading an article where the xanthophyll has a Rf of 0.16 (it seemed very polar to me) is it possible that the differences between your experiment and the experiment from the article (they used spinach) in the xantophyll are because there are many types of xanthophylls (like lutein and violaxanthin) in different plants? Or why did the xanthophyll in the spinach stood behind chlorophyll b and yours didn't?
I really appreciate your work 😊..ND need lots more videos like this
Clear and cut explanation with a well defined experiment. Thank you very much.
Thank you so much! Just by watching this video, I understood the principle of chromatography. Keep up the good work!
So easy to understand. Thank you.
I listen to this everyday before to I go to bed! Very calming voice and gives me great long sleep similar to death.
I have no idea what you’re actually saying tho ;)
Can’t wait for my chem test tomorrow!!!!
Goodluck on your test!
Aliia T thank you!
Hope you did good on your test lmao
tf
Very helpful during this pandemic👍
An excellent video lessons for layman like me..thank you
This is great thank you!
This is very easy and helpful experiment for our practical exam..
Great job... Well explained 👍
Thanks for showing best video
very helpful while i can not afford offline classes for practical during pandemic.
Thnx for the wonderfull expaination👍👍👍👍
Sir, Why should we remove mibrib?
Are there any substances released from mibrib that affect the pigments?
Wow you are a great asmr artist! This was so relaxing and helped me fall asleep easily! Keep it up man👍
Aliia T same!!!!
(in asmr voice) Happy to know that my videos have started to have more than one application; helping some in their bio while also taking the life out of others ;) (No pun-casm intended)
Thank you.Actually i was looking for this
Great video!! Very helpful
wahhhh bete moj moj moj ji karda very helpful video for student sir very good sir
Really helpful
Thanks😊🙏
Thank you for this helpful video
At 5:54 I just had a question
Can I say that the first pigment spot which has been mentioned as chlorophyll b does it contain only the pigment chlorophyll b or it is a mixture of all the other pigments as well and gradually only carotene is left
Each spot is a single phytopigment. From the first spot (chl b) the remaining pigments except chl b will migrate to the second spot (chl a spot). From second spot, all other pigments except chl a will migrate further up to the third spot and so on. This groups migration and the leaving behind of one pigment type at each "stop/station" will continue until all the pigments have been resolved into different spots on the paper.
Amazing explanation 👏👏👏👏👏👏👏👏👏
Hi can i kow what plant did u use ?
Very nice explanation
Why we only use petroleum ether: acetone combination instead of using any other solvent for chlorophyll
more helpfully thank u
Thx sir! It'll be really helpful for my practical exam tomorrow
Thanks a lot for helping me... 🙏
Thank you ☺️
Very useful 👍
Impressive video...
Hey, thanks so much for this video. I have a science fair project and I’m doing this but I’m not sure what I want to test and compare in my experiment. If you have any ideas please tell me. I would appreciate it a lot.
Hi, you can try leaves of different colours and shades. I've also seen some videos where they separate the pigments contained in ink. You can also separate amino acid components in a protein rich food source. Although they will need a visualizing agent to be sprayed on to them and then slightly heated to be able to see the amino acid spots on the paper. But for this u will need Ninhydrin (a potent carcinogen) so i highly doubt if thats the right thing for a sc fair projct. So u can work on Leaves and inks. Good luck 👍 i'm here if u need help.
Thank you so much man!!
thank you!
Ty sir good video
Good work 👏 ♥️
hello sir how to know that our paper chromatography is fail?
If u dont get the chlorophyll and carotenoid bands/spots. U need to recheck your solvent and use quality whatman paper.
What type of leaves hv nice colours to show on the paper?
You can perhaps use a mix of leaves of different colors; green, red, yellow, orange...... Or you can use a mix of different coloured inks. Never actually tried these "fun" experiments before though. Good luck 🙂 do lemme know the results. 👍🏾
thank you
Very inf. And helpful tq
what can be a substitute for the petroleum ether:acetone?
you may substitute it petroleum ether with something that is non-polar such as hexane.
What happened to the "My lab" video?
Had to pull it down for various reasons. Will come up with a better vdo in the foreseeable future 😁
Thankyou so much
Question, how are you able to determine the kind of pigment it shows??
standard textbook knowledge. This is a highschool level experiment performed as part of syllabi, based on experimental facts already investigated decades ago by pioneers in chromatography.
@@TheSingtangpaScienceGuy thank you very much
Thanks
Where is the measurement by the way?
so, where is the measurements?
May i know, what type of leaf it is?
i used amaranth leaf, as it was just nearby me. You can use any leaf as long as its green.
Nice video sir
hi there i did this experiment last week but the phaeophytin was invisible. why is that? is there any scientific reason for that ? thank you
What plant species, what solvent (or solvent combination), did u use?
@@TheSingtangpaScienceGuy we used Sauropus sp as the plant species and an ether acetone solvent
@@vishesharrvansashitharan3593 its probably because the leaves from which you prepared the extract, are still young, green and actively photosynthesizing. Pheophytin, as you might have already known, is one of the breakdown intermediate products of chlorophyll, and this breakdown into pheophytin, takes place usually during leaf senescence. So, even if the fresh green leaf might contain a little bit of pheophytin, the quantity is not so much as to to be seen on a chromatogram. Ofcourse, if you do a spectrophotometric analysis , you will detect some amount. But If, for any reason, you still want to see pheophytin on the paper, you can acidify the leaf extract prior to loading on the paper, in that way the chlorophylls lose their Magnesium and become pheophytin. Or you can make a leaf extract from old, senescing leaves that have yellowed. You are sure to find a nice pheophytin band/spot from old leaves. This is all i know from my textbook knowledge. Correct me if i'm wrong. Goodluck.
@@TheSingtangpaScienceGuy alright that was very helpful, thank youuu soo much , god bless you , this is the actual experiment for my practical test next week, again you've helped sooo much , thanks🙏
helpful!
Very good
Whts the ratio of acetone petroleum ether mobile solvent??
1:9
I thank God how as given you wisdom
Wow it blown my mind sir
thanks sir tomorrow my biology practical and IAM well prepared for that so thanks again...
I would like to know where are you from plz tell
Hope u did well in ur exam. I am from Manipur
Nice
plz ,Full notes send this experiment
Wow
🙏🙏👌👌
4.00
Hey Random People Scrolling Down The Comments
Vai zara zoor se bol yaar😕
Hi. Volume problem has been adjusted from subsequent videos. Enjoy !
Potangina gusto ko lang magsagot ng modules huhuhu
Thanks