2.9 Separation of Photosynthetic Pigments by Chromatography (Practical 4)

Instructional video demonstrating how to carry out chromatography of photosynthetic pigments in leaves (including taking measurements and calculating Rf values). This is a required practical in the IB Biology syllabus (Practical 4) for all students as part of Topic 2 Biochemistry. You can be examined on your understanding of this practical in Part A of Paper 3 of the final IB exams.

[@rondayaram5792]

Incredibly detailed. Well done. Thanks for sharing

[@a-171fac]

Absolutely helpful and on the point. Thanks. My lab practical is on it's way. It helped a lot

[@DeepikaSharma-io7bl]

Well done mam it's really very helpful video for us keep create video for us

[@Kwork1104]

Helpful video
Must all bsc students watch this before exam❤❤

[@shristiasylum]

Good job dear👍

[@nguyenkienam2331]

this helped me a lot in lab mixture separation report

[@37KA4]

Hi please can I contact you via email ..

[@breeze3430]

Thank you! This is a helpful guide for our Plant physiology laboratory activity

[@teacherjayzeelynvanessacor6889]

9
⁹k

[@drewhenderson8223]

Your welcome

[@idkhahahaha]

but what does the Rf value actually tell you about the pigment?

[@deminigamage9438]

Thank you so much! I didn't understand anything given in my lab manual. But this is very clear.

[@sabiha23467]

Thank you madam I understand very well

[@TheHexbugfan]

Ms. Webber sends her hellos!

[@climax7260]

Thank you it helps

[@peneloppedjeakoua1570]

Very useful for my experiment tomorrow

[@sashaart781]

Can I ask what solvent did she use? Please answer me 🙏

[@zmazn]

what should I do or what does it mean if the carotene keeps ending up being the last one below? I tried it 2 times and its always the same and im afraid the school lab wont let me do it again and i have to make a lab report about it with my picture in it.. I used spinach leaves btw

[@khushiparmar7444]

Solvent system which solution use??
Can you please tell me

[@phenyotshabang6394]

Can someone please explain why acid washed sand and anhydrous MgSO4 are used in the preparation of the extract of this kind of chromatography.

[@hotpepper7782]

Well my bio teacher said the acid washed sand was just there to make the grinding process easier about the anhydrous MgSO4 idk y it's there.

[@pawsitivebhumika6462]

Thanku so so much❤

[@siddikpk8385]

😊 🙏 thanks

[@misskhan8328]

your english is too good

[@kennyyong7694]

Excuse me, it is the same procedure for thin layer chromatography right?

[@Palak..15]

Yup:)

[@Idk-ij1yy]

Is there any way to improve this method

[@niloypaul34]

thank you

[@afolabifakanbi7782]

Tanks

[@Leen-ih8ql]

what are the methods or ways of separating plant pigments?? only TLC and paper chromatography ?? please I need help

[@videoteaching47]

u can do through chemical method as well.

[@judeigho7254]

Is the eluting solvent polar of non polar?

[@ayodelebello1145]

How much acetone is used for each leaf sample?

[@detss3795]

not just acetone 5 ml solvent which contain 9 part Petroleum ether and 1 part acetone

[@detss3795]

@Elijah Tam-od Ok

[@roshnimathew3209]

Instead of ether which solvent can be used?

[@Time12104]

Which solvent used?

[@kiransharma-oo2wq]

I think acetone

[@detss3795]

5 ml solvent 9 part petroleum and 1 part acetone sis

[@roshnimathew3209]

Instead of ether which solvent can be used?

[@anishakumarisingh581]

Plss give me the exact amount of solvent

[@geetaikalpande8471]

AND WHAT HAS SHE ADDED???

[@daphnesaipelekomiti7254]

Can someone please my lab

[@evelynt.britos2863]

What’s the significance and use of Rf value?

[@evelynt.britos2863]

After determining Rf, what’s next? Does it end in finding the Rf? Thanks.

[@martajackson11]

Hi, biology student here! From what I know, the Rf value is one of the methods you have to distinguish between the several photosynthetic pigments you can find on a leaf. You can also identify the pigments just by looking at their color. Basically if you have a Rf value close to 1, that means that the pigment moved quite a lot and it was abel to do that since it a polar pigment. If on the contrary you have a low Rf value, that means that the pigment didn't move that much and thus has non polar groups in its constitution. Please correct me if I am wrong

[@xyrildanmanuel783]

hi chemistry student here. the Rf value is one of the information we use to pinpoint what the pigment might potentially be. chemists around the world do this chromatography on initially known compounds and they share and confirm the Rf values they have obtained in a database (or multiple databases like the one presented at the end of the video) so that future analysts would have a reference when repeating the same chromatography on the same compound. think of it like a pseudo-fingerprint of a certain compound although i believe not all compounds have unique Rf values

[@ahappycoder2925]

RF value is used to identify the pigment

[@jimsamson7934]

It allows you to get a measurement for 5G or 3G radiation frequencies. That way, you can gauge how thick your tinfoil needs to be in order to block those signals

[@ammarsyah267]

thanks ..
"a few drops of acetone" 🗿

[@keerthicreations2153]

Hii

[@keerthicreations2153]

FF

[@keerthicreations2153]

FF

[@keerthicreations2153]

FFFFFFFFFFFFFFF

[@detss3795]

@@keerthicreations2153 hi yes What u want to ask

[@randomgaming9869]

Result

[@realism6793]

wat the pigments i got 10 mins

[@ranaumararshad3351]

Tare pan nu lun