you didnt show the place where the 1550 and 1450 fragment shoud have been added to the plasmid....you made two plasmid on with only Enzyme 1 and 2. can you please add it to the plasmid
Awww.. looks like you missed it. Ok, remember a specific restriction enzyme only cuts at a specific DNA sequence. So mix your DNA with that enzyme, and you will find the cut- the location. If you have more than one enzyme, you can build "a map" of restriction sites- cuts. Here's an analogy: take a circular string, cut it into different length pieces, then try re-assembling the same circle... like a puzzle. The location where the pieces meet- is the location where the specific enzyme cuts.
very helpful
you didnt show the place where the 1550 and 1450 fragment shoud have been added to the plasmid....you made two plasmid on with only Enzyme 1 and 2. can you please add it to the plasmid
this was very helpful thank you so much!!
a very great job
This was very helpful!
Thank you so much... This video helped me solve a problem I've been battling with... Wonderful Job🎉
So glad I could help
Wow thanks 👍🏻👍🏻
You’re welcome 😊
What if E2 also gives two fragments. How can we do the mapping then?
Thanks a bunch 🌟🌟
you did not add enzyme 3 to the final map, is there any reason for that?
Not sure what you mean. Enzyme 3 is definitely there :)
You didn’t show how to find the specific location of the restriction enzymes
Awww.. looks like you missed it. Ok, remember a specific restriction enzyme only cuts at a specific DNA sequence. So mix your DNA with that enzyme, and you will find the cut- the location.
If you have more than one enzyme, you can build "a map" of restriction sites- cuts. Here's an analogy: take a circular string, cut it into different length pieces, then try re-assembling the same circle... like a puzzle. The location where the pieces meet- is the location where the specific enzyme cuts.