DONATED !!! Today I passed the final exam successfully in the subject of “ Horizontal Gene Transfer “ in medical school because of your video lectures !!! I can’t find the words to thank you honestly!!! Without you , I would have been lost or failed!!!
Andrey, really appreciate your videos; specifically writing down the sequences and showing with different colors really makes it easy to understand. Thank you very much!
just 2 minutes into ur lecture I had to pause the video and Comment on how informative this video was !!! This is amazing! I loved it ! Thank you so much, & keep doing what you do !
I'm seriously mind blown now..like all these were really confusing but after hearing this lecture everything connects and makes sense..Thank you so much
Thank you! I keep reading my book and looking up each section in your videos because I can't understand my book. Thank you for your great illustrations and repetition. You have a gift.
I go to school in Iran and English is my second language, but i understood this lecture more than i learn while reading my own biology book! Thank you so much!!!
Using this exellent videos of you , i can make more effective my studying and it takes to me less time to clearly understand things. I just wanted to thank you for your contribution to community, especially to students!
Thanks for this video! It was really informative but also simple to understand, you included a lot of information that put all of the little parts into perspective.
The ligase can rejoin the original DNA strands as well as it can form recombinants, which can be identified and separated by some techniques. To avoid self ligation of the DNA molecules, an enzyme called Alkaline phosphatase can be added to the DNA fragments before ligation. This enzyme removes the 5' phosphates from the DNA segments. Now the DNA fragments can only attach through h- bonding between the sticky ends of the DNA molecules. Inside the bacterial cell The DNA repair systems join the single stranded nicks. The recombinants can be purified later.
Great videos! Your teaching style is exceptional! They definitely clear up many of my questions in my readings. However, I did have one question pertaining to this. When you say that DNA ligase is added to combine the two sequences of DNA and form the recombinant DNA, how do you make sure that DNA ligase doesn't just form the two original circular forms of DNA?
I am probably too late to reply but... A phosphatase comes to take dephosphorylate the plasmid (so it doesn't reanneal) When the gene if interest comes along, it binds to the plasmid by its phosphates.
Very nice video, thank you :) I think there is a mistake though. The 5' and 3' sticky ends do not match in the upper left drawing and lower left drawing.
One thing I don't understand.... What makes the sticky ends of the two molecules bind to each other rather than just close their own molecules again once the DNA ligase is in the mix?
thanks a lot very well understanding..but I have a question, we say that bacteria use these restriction enzymes to digest the viral dna when infected by them and before doing that uses methyl transferase enzyme to protect it's dna from these restriction enzymes...my question is, are these enzymes always present in bacterial cell or they will be produced up on infection??? is there any mechanism for that ?? best regards
Mustafa Fahmi انا كنت هسقط اقسم بالله لولا الفيديوهات بتاعته !!! رهيب نجحتي فسنه اولي و سنه تانيه طب فمواد البيوكمستري و المولوكيلور بيولوجي و الهوريزونتال جين ترانسفير !!! عبقري !!! يستحق فلوس الدراسه مش الجامعه و الله
This is the best biotechnoogy lecture series I have ever watched.
You really are a good teacher. Thanks for doing this!
I wish I discovered your lectures earlier! Very good stuff!
And I see this 4 years later
@@tejasvisakhamuri1380 And I see this 4 years later
Amazing..I was skeptical at first but u did it for me..youre a great teacher..thank you
+nick smith thanks for giving it a shot! :)
great lectures sir..u always do well😀👍👍
*skeptical
DONATED !!! Today I passed the final exam successfully in the subject of “ Horizontal Gene Transfer “ in medical school because of your video lectures !!! I can’t find the words to thank you honestly!!! Without you , I would have been lost or failed!!!
Andrey, really appreciate your videos; specifically writing down the sequences and showing with different colors really makes it easy to understand. Thank you very much!
BEST explanation with details EVER, you really understand what your are explaining. Thank you, we needed you.
I love you, man. Like you’re saving me because your ten minutes videos are equal to a 2hr class. Than you.
Happy to hear it! :)
honestly just made everything so much more clear for me... no idea what any of this meant until watching this thank you !
How did I not find you before? You restored my faith in teachers. Absolutely brilliant.
I am truly have a crush on you AK. You are better than any professors i ever had. This is how you teach, not reading off the slides to your students.
I just love your way of teaching and especially the content!
You are absolutely amazing! You are saving someone whose in Egypt right now. Didn’t understand anything from my teacher, thank god you exist!
The same I also didn't understand my lecturer i am from Kenya 😂😊
Thank you, AK LECTURES. The best biology channel I´ve seen
just 2 minutes into ur lecture I had to pause the video and
Comment on how informative this video was !!! This is amazing! I loved it ! Thank you so much, & keep doing what you do !
I'm seriously mind blown now..like all these were really confusing but after hearing this lecture everything connects and makes sense..Thank you so much
Absolutely LOVE this video. Thank you for making hard topics easy to understand! You make studying for the MCAT a lot easier.
you have seriously gotten me through pre-med . i owe you my life
Thank you so much for creating this channel. Your videos are helping to get me through biochemistry.
Thank you! I keep reading my book and looking up each section in your videos because I can't understand my book. Thank you for your great illustrations and repetition. You have a gift.
I go to school in Iran and English is my second language, but i understood this lecture more than i learn while reading my own biology book! Thank you so much!!!
how is the coronavirus
keep on the good work, everytime i watch your lectures before exams i always pass
Still amazed!
Best lec. of biotec ❤ all the concepts becomes crystal clear. Thank you sir☺☺
Using this exellent videos of you , i can make more effective my studying and it takes to me less time to clearly understand things. I just wanted to thank you for your contribution to community, especially to students!
Without your lectures many concepts won't be that clear so thank you so much for effort
Thank you very much
You are my real teacher
I've followed your videos since year 2 and I'm at the end of 4year
Greetings from Brazil!
Your lessons are really very good! It seems to be easier after that, and you have a perfect "blackboard".
Thanks a lot!
João Carnero Guedes Thank you! glad to see they're helping you! :)
Lecture is 10/10.
Legendary teaching.
Wooow....it is explained so clearly and the explanatory diagrams are best .Great teaching sir..Thanks!
excellent way of teaching .....that was amazing .....now my concept is crystal clear thnx sir.....
I hate genetics but you are the best lecturer i have ever heard. Loved and understood each of your videos 😀 Thank you!
Ur excellent i wish u were my biology teacher
Omar Abouraya Thanks Omar! :)
same here 😔
Hands down best on youtube.
Everytime I search for my topic you come up...What DON'T you know
P.s you're a great teacher!
So easy to follow and understand. Thanks for taking the time.
Good lecture
very help fully
OMG you are the best. brilliant way of explaining hard subjects.
Hello AK...I'm back and as usual your videos give me a positive energy to go through my midterms 😄 I can't say how much do I love you ❤️
All ur videos are really helpful
Thanks from Egypt 👍
I love your lectures. Glad I found these. I do recommend using a different colored t-shirt though. White on white is jarring to my optical nerves.
Thanks for this video! It was really informative but also simple to understand, you included a lot of information that put all of the little parts into perspective.
Thank you! You work so hard and deliver everything so well!!!
The ligase can rejoin the original DNA strands as well as it can form recombinants, which can be identified and separated by some techniques.
To avoid self ligation of the DNA molecules, an enzyme called Alkaline phosphatase can be added to the DNA fragments before ligation. This enzyme removes the 5' phosphates from the DNA segments. Now the DNA fragments can only attach through h- bonding between the sticky ends of the DNA molecules. Inside the bacterial cell The DNA repair systems join the single stranded nicks. The recombinants can be purified later.
I am watching this video in 2024 and it helped me a lot. Thank you so much.
You are amazing and make us understand topic fully 👏
Very clear lectures thnx ...AK LECTURE
You are amazing! Thank you so much @AK LECTURES
Keep up the good work!
I adore the way he lectures
bushrahussein thanks! :)
thank you so much for this video! i have a genetic recombination midterm exam tomorrow. This video really helped me :) wish me luck!
Very well done sir, big shoutout from Japan!!
Very interesting, The lecture is well digested, thank you.
This was so helpful, and very well explain, thank you so much you saved me from getting a bad grade on my exam
this awesome! am really enjoying all of your class lectures, keep it up sir.
THIS IS SO HELPFUL
Thank so much 😍 I'm studing genetic engineering right now stage one and you're vedios really usefull for me ..great teacher 😁
Thank you so much.. You just saved my life!! You're really a great teacher
Great videos! Your teaching style is exceptional! They definitely clear up many of my questions in my readings. However, I did have one question pertaining to this. When you say that DNA ligase is added to combine the two sequences of DNA and form the recombinant DNA, how do you make sure that DNA ligase doesn't just form the two original circular forms of DNA?
Shayan Alam i have the same question
I think the DNA Ligase will join the DNA through reforming the bond
between the 5′-PO4 coming from the insert and the 3′-OH of the
plasmd
I am probably too late to reply but...
A phosphatase comes to take dephosphorylate the plasmid (so it doesn't reanneal)
When the gene if interest comes along, it binds to the plasmid by its phosphates.
What amazing explanation dude
Thanks alot.
Which enzyme cleaves hydrogen bonds when the restrictions enzyme cleaves phosphodiester bond , to separate the DNA segment ?
great work!thanks a lot. it helped me for my biology olympiad!
This make sooooo much more sense!! Thanks man 👍🏼👍🏼
You are awesome really, thank you for your lectures
Amazing video, great explanation. I don't understand why anyone would dislike this lol. Thanks!
You ARE my professor!
Love your lectures...thank you.
Great teaching. Thanks very much.
Very nice video, thank you :) I think there is a mistake though. The 5' and 3' sticky ends do not match in the upper left drawing and lower left drawing.
I fall in love with everything in ur video :) god bless u💕
Tabarak Ali is it clear to you
farah baqer haha! Omg ... that comment though!
thank you so much...you are such a good teacher.
Thanks mate, you're video's are very helpful
perfect AK as compare to bollywood AK.
Saviour, thank you!
Pretty detailed and very informative. Thanks a mil
You're just infinitely great. Thank you a lot.
awesme teacher.... well understood
This is so interesting!!! I had no idea of how useful genetics could be :)
Thank you. I wish I discovered your channel earlier
Thank you soo much for the explanation🌻🌻
Big thank you from France! :D
woow ,verrrrry gooooood ,iam biology teacher from KSA, thak u .i relly nedded this
123 4 awesome! :-) good luck with your class!
Amazing teacher !!
OMG YOU'RE A LIFE SAVER OMG
Thank you....from Kashmir
perfect! Amazing! very smart Lecturer. Thank you so much for uploading..You presented it as a piece of cake.
+alexander debru glad it seemed that way :)
Quite impressive!
He is great but, my, the way he speaks!
I can’t thank you enough man!!
Can you add a picture of the white board linked in the comments? Thanks by the way, I would have failed biochem without your video series.
I really loved the new beautiful videos with colors .. big like for that 😍
dyoomah17 Thanks! glad you appreciate the aesthetics ! :)
I freakin love you. Don't stop makin videos. Cause i dont wanna stop lovin u
thanks! i wont :) also your video with the two cats is hilarious.
@@AKLECTURES yaaay. You checked it out? Heehee thankies.
One thing I don't understand.... What makes the sticky ends of the two molecules bind to each other rather than just close their own molecules again once the DNA ligase is in the mix?
Great job.
thanks a lot very well understanding..but I have a question, we say that bacteria use these restriction enzymes to digest the viral dna when infected by them and before doing that uses methyl transferase enzyme to protect it's dna from these restriction enzymes...my question is, are these enzymes always present in bacterial cell or they will be produced up on infection??? is there any mechanism for that ??
best regards
Mustafa Fahmi
انا كنت هسقط اقسم بالله لولا الفيديوهات بتاعته !!! رهيب نجحتي فسنه اولي و سنه تانيه طب فمواد البيوكمستري و المولوكيلور بيولوجي و الهوريزونتال جين ترانسفير !!! عبقري !!! يستحق فلوس الدراسه مش الجامعه و الله
Dude this is really good. Thx man.
T Math thanks! :-)
Awesome job. keep it up!
thank you very much doctor you are the best
THANKS FROM NZ
why can't we use PCR for amplification?