Antibacterial Activity Test by Disk Diffusion Method_A Complete Procedure (Kirby and Bauer Method)
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- เผยแพร่เมื่อ 22 พ.ค. 2024
- Antibacterial Activity Test is a very common & important microbiological test parameter by which we can determine the efficacy of any antimicrobial substance. This video represents a complete procedure of Antibacterial Activity Test. __________________________________________________________________________________________
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Your channel is very nice I am thankful to you as i recalled all the methods i forget, by watching your videos.
Thank you for videos such as these! You are the best. Learning a lot from your videos. Can you do Antimicrobial protection (Preservative efficacy) test for cosmetic samples? Thank you so much and keep up the good work. More power to you.
Thank you so much for your support. We will make video for preservatives efficacy test later. Stay with us.
Thank u so so much for such an amazing information
Thank you
Very Helpful. Thank you.
You are welcome
Sometimes it happens that the zone of inhibition isnot very clear, 2 shades are present,qhy does it happen and what should bedone,how should we measure the zone of inhibition me
Really very good very easy and simple way
Thank you
Excellent demonstration and explanation. Pl continue with nanobiotics
We will try with that
Share Antibacterial activity of CuO nanoparticles plz
Hi, thanks for the great video. I am trying to culture Pediococcus acidilactici on MRS Agar and see its resistance to a certain compound. How can I make a paper disc specific to the compound I want? Do I just dip the paper into my mixture?
Yes, absolutely
Well done 👍
Thank you 👍
Hi there, please help! I am conducting the antibacterial assay of my plant sample following the well diffusion method. But I have faced with a problem. Should I filter my plant sample with the help of 0.2 micron filter inside laminar flow cabinet before adding the samples into the wells. I found that when I filtered my sample, the concentration as well as the volume of my prepared sample also get decreased. I have also gone through severe literatures of my field but found no paper mentioning the use of 0.2 micron filter. So is it necessary to do so or I can skip this step?
No. You can do the test without filtering your sample. Though chance of contamination may remain, you can use 0.45 micron filter.
wonderful
Thank you
Awesome thanks
Thanks
Hi, Nice video. I am totally naive in this. I want to check the antibacterial activity of silver thin films and small scaffolds. Will you please guide me? Is it necessary to use Amoxycillin antibiotic disk along with our sample?
Antibiotic disk is necessary to check the validity of the test result.
But you can do this test without using antibiotics disk.
Hi! I am still quite naive in this topic. I have two questions I wanted to ask :
1. if I am testing an antifungal activity of a substance (garbage enzyme from citrus peels to be specific), can I use well diffusion method?
2. do I inoculate the fungi in the same way as shown how to inoculate the bacteria in the video (by streaking the entire surface of the plate)? Or is there a better way to do it with fungi (ascomycete)?
I am using Botrytis cinerea as the fungi btw.
From what I can understand so far (by researching the topic on the internet), the standardized/more reccomended method for testing against fungi is to use broth dillution, but I am also under the impression that well diffusion can also work to test antifungal activity againts Botrytis cinerea, from a study I had found titled "A Comparative Study of Antimicrobial and Antioxidant Activities of Plant Essential Oils and Extracts as Candidate Ingredients for Edible Coatings to Control Decay in ‘Wonderful’ Pomegranate"
Sidenote : thank you so much for the content you post here, as a student I find them extremely useful. Please know that is perfectly understandable if you don't find the time or need to respond to my very specific questions, and sorry for taking your time 😄
You can check antifungal activity by disk agar diffusion method. Only difference is the inoculation system. Collect spore and dilute in saline water and inoculae into agar surface. Rest of the method are same. We will upload a new video for it. Stay with us.
@@MicroChemsExperiments Omg thank you so muchhh 😄
This video was very helpful. However, I wanted to ask about the sample used and if you know any other samples that can be used in this kind of experiment.
I used an antibiotic disk and a treated fabric sample. You can analyze any sample with antimicrobial properties
Hey I'm working on a fabric treated with copper nanoparticles (turns out I actually synthesised copper *micro*particles but let's not talk about that). Do you think I can use this method to test the antibacterial activity? I've been searching for methods and was thinking between ATTCC 147 and JIS L 1092 but I've figured out my fabric samples were not fit with aatcc 147 and I couldn't find enough information about jis l 1902. I would appreciate some help :)
You can use this method to check the antibacterial activity of any treated fabric sample. You can use any bacterial strain and there will be no problem with it.
@@MicroChemsExperiments is the size of the fabric optional or should I use 6mm × 6 mm like you did?
@@stuckonthepuzzle using 6mm×6mm is better but you can use any other size.
@@MicroChemsExperiments thanks a lot!
@@stuckonthepuzzle you are most welcome
Thank you
You are welcome
Very good
Thank you
Hello! I've got a fabric treated with silver nanoparticles. Do I need to sterilize my samples before I test the antibacterial activity? If so, how do I sterlize them?
No. Your sample already have the antimicrobial property. So no need to sterilize the sample. But during the development of nano particle, you should take measures to avoide much contamination.
@@MicroChemsExperiments thanks!!
@@ece9655 Welcome
thank you
You're welcome
how about antifungal activity for seaweeds against m.fijiensis can be the same method? also the agar will be PDA
No. Not same. Fungal spore suspension is preprepared and the rest will be the same. SDA, PDA or DRBC can be used.
We will make the video for it soon.
Nice video😍 one question though What if the sample is liquid ?pls help me out 🥲🥲
For thoes who don't know we can use either one Nutrient agar or muceller hinton agar or Tryptone soya agar for in the petri dish
SUPER COOL
Thanks
Could one use a block sample of a filter paper treated with a sanitizer
Yes. You can use
Hi,, your video is so nice.. im here to know what method should i use to to test the effectiveness of anti bacterial hand soap that we are to going to make. Can you please share me a tip or method that i should use to determine if the antibacterial handsoap that im going to make is really effective for hands and how would i make it.(school research purposes only) your reply would really be appreciated... Thank youuu
Use a sterile black disk and soak in you soap sample solution. Then using this same experiment you can analyze the antimicrobial activity.
Sorry for late reply
@MicroChem's Experiments How can someone do the Antibacterial Activity Test for a powdered sample for example termite sample?
For powder sample, you can make a solution by dissolving your sample in a suitable solvent.
Then prepare the MHA plate inoculating with bacterial.
Now bore the media with the aid of sterile borer or something else and in this bore add 50 microliter sample solution. Then follow rest of the procedure according to this video.
how did you made the pure culture plate of staphylococcus aureus?
By steak plate technique using a reference culture
Dear Madam..u r doing well job but if u will explain addition of chemical.i.e why we are doing this chemical and reason behind it than it will be more helpfull to all..tx a lot
@Parmjeet Singh, wel said. I have plan to do it what you are saying. I will make additional videos for every test explaining the machanism and chemical reaction. Please give me some time and stay with me. Thank you.
Hi can u please show any video for antifungal activity by well diffusion method
Sure. We will make video on it. Stay with us.
@@MicroChemsExperiments have you made for antifungal activity ..pls do for black fungus
How to do antibiotic susceptibility test for Onion..can you make a video
Yes, soon
Educative
Thank you so much
What was the purpose of preparing Trypton soya agar? Or I'm missing something. Plz help! Overall video was very very useful. Thanks a lot and keep going.
TSA is needed to prepare pure culture of reference strain.
Ma'am how do you prepare the pure culture colony of bacteria?
Follow this method: th-cam.com/video/bm99zrq3ijo/w-d-xo.html
Hello! I know this comment is kinda late, but can this study use for testing of the effectiveness of antimicrobial gel of band aid?
Obviously you can use.
@@MicroChemsExperiments thank you so much! ^^
@@kiraolisatosa2636 you are welcome
@@MicroChemsExperiments oh btw, will there be no complication on it if we also use seaweed to make the gel bandaid and test it on this method? We can't find some sources in google and we lack sources in our city that's why. :
@@kiraolisatosa2636 No problem. You can use this method for that purpose.
Dear ma’am waiting for fungal susceptibility test especially for black fungii
Thank for recall my mamories. We will develop video soon.
Is it necessary to use MUELLER HINTON AGAR
You can also do this experiment using TSA or Nutrient Agar
Maam why we are using 2 types of agar here...and what is a nutrient agar ..are these examples for nutrient agar? I belong to chemistry background and dont have any idea about this method..but i need this method for my project .
Different media are used on different purposes. Study using Google resources
Hi! where did you use the TSA?
TSA is used to prepare a fresh culture of a specific bacterial which is not shown in this video
Maam, how much agar that we can make with 38g of MHA medium ? in the video, i only can see 1 complete agar plate? if i need to make about 8 agar plate, how much do i need to use? Thank you in advanced
In the media presentation, you need 25ml liquid MHA solution for each plate. So if you want to prepare 8 plates then you need total 200ml liquid MHA solution.
To prepare 200ml MHA solution, you need 7.6g MHA dehydrated powder (as 38g needed for 1000ml or 1L solution. So 7.6g needed for 200ml solution).
@@MicroChemsExperiments Thank you so much maam
@@nurafilashahira8610 you are welcome
@@MicroChemsExperimentsmam what is the use of tryptone soya agar we have seen the use of soya broth and mueceller hinton agar not that one?
Pls can TSA be used as the pure culture instead of TSB
Yes. You can
Maam I have made antimicroboal herbal handwash and now howI will check its antimicrobial activity
You can follow this method for checking the antimicrobial activity of your handwash sample
Can I perform the same test for anti-microbial activity ?
Yes
How to make a streptomycin disc? Please help me
1. Purchase sterile blank disk from Oxoid.
2. Prepare streptomycin solution.
3. Soak the blank disk into the streptomycin solution.
3. Dry the disk in air.
Use after drying
@@MicroChemsExperiments mam what is the temperature to be kept for drying the disc in hot akr oven?... For how many days
Ma'am, can you please let us know how the block of the sample was prepared?
It was a fabric sample treated with Silver Nano Particle.
@@MicroChemsExperiments ma’am can you please make a video on that also please ma’am
@@shwetapotdar6480 sure. We will prepare. Stay with us.
Can we use 100 microliter of bacteria solution instead of using swap??
Which doesn't follow the reference method but maybe you can try in that way.
can someone tell where TSA medium used?
Yes even me in want to know
I get it bro we can use any one either nutrient agar or muceller hinton agar in the plates
I still waiting for analysis video
Of sugar percentage in any samples
Please 🙏🏻🙏🏻🙏🏻🙏🏻
Thanks for being with us
Plz phasforus test in laboratory plzz
We already uploaded the test video.
Video link: th-cam.com/video/syTwwvQAA5I/w-d-xo.html
What is that sample
Treated fabric silver nano mat
THANK YOU MAM , BUT ONLY ONE WORD CAN CHANGED YOU CAN WRITE (DISK) THESE IS NOT RARE WORD THE WORD IS (DISC)
Both words are same in meaning. So you can use whichever you wan.
Plz remove background music
lol nothing about this video is sterile.
Oh, really? 🤣🤣
I still waiting for analysis video
Of sugar percentage in any samples
Please 🙏🏻🙏🏻🙏🏻🙏🏻
Please give us some more times. We will make it soon.
Ok thanks for notice to me🙏🏻
@@Wardi_lovers You are most welcome.