How to do a Kirby-Bauer Disk Diffusion Antimicrobial Susceptibility Test

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The purpose of the Kirby-Bauer disk diffusion susceptibility test is to determine the in vitro susceptibility of bacteria and fungi to various antimicrobial compounds. It is a standardized test that can be performed in a laboratory setting and is a valuable tool for both clinicians and microbiologists. The main benefits of this test are the flexibility of drug selection and obtaining overnight results. It is used to help guide the selection of antimicrobials for the appropriate treatment of infections caused by bacteria and fungi.
The test is performed by placing antimicrobial impregnated filter paper disks on the surface of an agar-containing medium that has been inoculated with the patient’s microorganism. Upon contact with the agar surface, the antimicrobial agent immediately diffuses from the disk into the surrounding agar where it may inhibit the growth of the microorganism. If the microorganism is inhibited by the antimicrobial agent, a clear zone of ‘no growth’ will be present around the paper disk after incubation. The presence or absence of a zone of inhibition around the disks is a measure of the microorganism’s susceptibility to the antimicrobial agent.
The Kirby-Bauer disk diffusion test is a standardized method for determining the susceptibility of bacteria and fungi to various antimicrobial compounds. It is a valuable tool for clinicians and microbiologists and can be used to help guide the selection of antimicrobials for the treatment of bacterial infections. By following these steps and referencing the relevant standards for current procedures, recommendations, and interpretive criteria, you can ensure that your Kirby-Bauer disk diffusion tests are accurate and reliable.
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Mueller Hinton Agar, for susceptibility testing (Kirby-Bauer) Cat. no. G45
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Mueller Hinton Agar (MHA), for Kirby Bauer susceptibility testing Cat. no. H11
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Blood Agar Plate, 5% Sheep Blood in Tryptic Soy Agar (TSA) Base Cat. no. A10
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ReadyRack™ Tryptic Soy Broth (TSB), USP, 10ml, Glass Tube Cat. no. K82BX
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McFarland Latex Standard # 0.5 Cat. no. ML05
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Wickerham Card, for Turbidity Adjustments to a McFarland Standard, 2x3 inches Cat. no. Z08
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ANTIMICROBIAL DISKS
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Filmed and Edited By Brian Tom
00:00 History
00:22 Benefits
00:41 Overview
01:40 Susceptibility test interpretive criteria
03:58 Materials needed
04:40 Procedure starts here
05:24 McFarland standard
06:49 Streak Plate
07:41 Place disks
09:02 Incubation
09:31 View results
10:07 Measuring
10:48 Refer to breakpoint tables
11:06 Conclusion
11:45 About us
Tags:
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[@mel-j]

What happens if you keep for more than 15 minutes?

[@HardyDx]

@mel-j The 15 minutes recommendation is part of the general guidelines provided by CLSI for a standardized procedure. It is important to use optimal inoculation suspensions, media, and incubation conditions for the most accurate and repeatable results. Please refer to the CLSI website and guidelines for more information.