How to do a Kirby-Bauer Disk Diffusion Antimicrobial Susceptibility Test

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  • เผยแพร่เมื่อ 14 มิ.ย. 2024
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    The purpose of the Kirby-Bauer disk diffusion susceptibility test is to determine the in vitro susceptibility of bacteria and fungi to various antimicrobial compounds. It is a standardized test that can be performed in a laboratory setting and is a valuable tool for both clinicians and microbiologists. The main benefits of this test are the flexibility of drug selection and obtaining overnight results. It is used to help guide the selection of antimicrobials for the appropriate treatment of infections caused by bacteria and fungi.
    The test is performed by placing antimicrobial impregnated filter paper disks on the surface of an agar-containing medium that has been inoculated with the patient’s microorganism. Upon contact with the agar surface, the antimicrobial agent immediately diffuses from the disk into the surrounding agar where it may inhibit the growth of the microorganism. If the microorganism is inhibited by the antimicrobial agent, a clear zone of ‘no growth’ will be present around the paper disk after incubation. The presence or absence of a zone of inhibition around the disks is a measure of the microorganism’s susceptibility to the antimicrobial agent.
    The Kirby-Bauer disk diffusion test is a standardized method for determining the susceptibility of bacteria and fungi to various antimicrobial compounds. It is a valuable tool for clinicians and microbiologists and can be used to help guide the selection of antimicrobials for the treatment of bacterial infections. By following these steps and referencing the relevant standards for current procedures, recommendations, and interpretive criteria, you can ensure that your Kirby-Bauer disk diffusion tests are accurate and reliable.
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    Mueller Hinton Agar, for susceptibility testing (Kirby-Bauer) Cat. no. G45
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    Mueller Hinton Agar (MHA), for Kirby Bauer susceptibility testing Cat. no. H11
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    Blood Agar Plate, 5% Sheep Blood in Tryptic Soy Agar (TSA) Base Cat. no. A10
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    ReadyRack™ Tryptic Soy Broth (TSB), USP, 10ml, Glass Tube Cat. no. K82BX
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    McFarland Latex Standard # 0.5 Cat. no. ML05
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    Wickerham Card, for Turbidity Adjustments to a McFarland Standard, 2x3 inches Cat. no. Z08
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    ANTIMICROBIAL DISKS
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    Filmed and Edited By Brian Tom
    00:00 History
    00:22 Benefits
    00:41 Overview
    01:40 Susceptibility test interpretive criteria
    03:58 Materials needed
    04:40 Procedure starts here
    05:24 McFarland standard
    06:49 Streak Plate
    07:41 Place disks
    09:02 Incubation
    09:31 View results
    10:07 Measuring
    10:48 Refer to breakpoint tables
    11:06 Conclusion
    11:45 About us
    Tags:
    kirby-bauer method of antibiotic sensitivity testing, antimicrobial susceptibility testing, kirby bauer disk diffusion method, antimicrobial susceptibility testing microbiology,antibiotic susceptibility testing,antibiotic sensitivity test, antibiotic susceptibility testing microbiology, antimicrobial susceptibility test, disk diffusion method microbiology, susceptibility test, zone of inhibition test,antibiotic susceptibility testing by disk diffusion, disc diffusion method
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ความคิดเห็น • 2

  • @mel-j
    @mel-j 5 หลายเดือนก่อน

    What happens if you keep for more than 15 minutes?

    • @HardyDx
      @HardyDx  3 หลายเดือนก่อน

      @mel-j The 15 minutes recommendation is part of the general guidelines provided by CLSI for a standardized procedure. It is important to use optimal inoculation suspensions, media, and incubation conditions for the most accurate and repeatable results. Please refer to the CLSI website and guidelines for more information.