Disk Diffusion Test (Kirby-Bauer Test)

แชร์
ฝัง
  • เผยแพร่เมื่อ 14 มิ.ย. 2024
  • In this video, Biology Professor (Twitter: @DrWhitneyHolden) will cover the Disk Diffusion test, also known as the Kirby-Bauer test, and discusses the background information necessary to understand how this test works. Specifically, this diagnostic test uses a highly standardized procedure to assess the level of effectiveness of different antibiotics against a particular bacterial isolate, by measuring the size of zones of inhibition.
    Want a PDF of the final white board with all the answers written in? Download FREE STUDY NOTES here: tinyurl.com/BiologyProfessorN...

ความคิดเห็น • 69

  • @areebfatima8125
    @areebfatima8125 ปีที่แล้ว +2

    Wow! You have such an excellent teaching method. Goes straight into the head without creating any confusions.
    Please keep uploading videos 💚

  • @Richardscreoleblend
    @Richardscreoleblend 2 ปีที่แล้ว +4

    Thank you, Professor. This is the third of your videos that my instructor has shared with us in micro lab this semester. I love your videos.

  • @ghita7971
    @ghita7971 5 ปีที่แล้ว +5

    The explanation was easy to follow and very clear! Wish my professor explained it like that!

  • @shahabfarooqi59
    @shahabfarooqi59 4 ปีที่แล้ว +1

    Concise explanation with impressive personality.

  • @jameskiiru7966
    @jameskiiru7966 4 ปีที่แล้ว +1

    Excellent young professor. Keep up the good work.

  • @marcelkenion8201
    @marcelkenion8201 5 ปีที่แล้ว +1

    You are wonderful, thank you for making all your videos.

  • @yesisantos1999
    @yesisantos1999 3 ปีที่แล้ว +1

    You make microbiology so much easier to understand. Thank you for this video!

  • @honeypott01
    @honeypott01 5 ปีที่แล้ว +22

    Very INEFFECTIVE sensitivity should be very effective sensitivity

    • @nikoscharalampidis7153
      @nikoscharalampidis7153 4 ปีที่แล้ว +2

      Yes, guys we got it, it is written 'ineffective', but she said again and again EFFECTIVE. Move on with your lives.

    • @luwamisaak2231
      @luwamisaak2231 3 ปีที่แล้ว

      I was confused too when she said it effectet

    • @idk123
      @idk123 3 ปีที่แล้ว

      @@nikoscharalampidis7153 ok.

  • @olenak3112
    @olenak3112 3 ปีที่แล้ว +1

    Thank you so much, you are real life saver!

  • @AmitisL
    @AmitisL 3 ปีที่แล้ว +1

    Very helpful , thank you so much

  • @jocelync8236
    @jocelync8236 5 ปีที่แล้ว +1

    thank you so much! best explanation

  • @Kam16665
    @Kam16665 4 ปีที่แล้ว +1

    Very clear explanation! Thank you! :-)

  • @mieshahbacker
    @mieshahbacker 4 ปีที่แล้ว +1

    This really helped me

  • @rahmirahmawati4551
    @rahmirahmawati4551 3 ปีที่แล้ว +1

    Thanks for the explanation luv

  • @Thinker-gd8ck
    @Thinker-gd8ck 3 ปีที่แล้ว +1

    Thanks for sharing

  • @arshdeepbhangu8884
    @arshdeepbhangu8884 28 วันที่ผ่านมา

    most beautiful and helpful teacher ❤

  • @samahahmed1673
    @samahahmed1673 3 ปีที่แล้ว +1

    Thank you so much 🌹🌹🌹

  • @rn_jay3257
    @rn_jay3257 5 ปีที่แล้ว +2

    Thank you so much! Now I understand!! ;)

  • @thapelomotsabi2742
    @thapelomotsabi2742 4 ปีที่แล้ว +7

    It took only 9 minutes to understand this after thoroughly reading the lab manual for hours♥️♥️

  • @sallyrahma608
    @sallyrahma608 2 ปีที่แล้ว +1

    Thank you

  • @ptilongmarchimrankhan3678
    @ptilongmarchimrankhan3678 5 ปีที่แล้ว +1

    It was very helfpul ...love you maam

  • @muhammadsalihuabdallah2781
    @muhammadsalihuabdallah2781 4 ปีที่แล้ว +1

    very interesting

  • @ofoxofox1
    @ofoxofox1 5 ปีที่แล้ว

    Please could you also cover the linear regression for MIC estimation, based on the halo results?

    • @BiologyProfessor
      @BiologyProfessor  4 ปีที่แล้ว

      I will add it to my list! Thanks! For now, this might help: academic.oup.com/jac/article/61/6/1295/739753

  • @priyapinky468
    @priyapinky468 3 ปีที่แล้ว +1

    Tq u so much madam.....

  • @damankaur105
    @damankaur105 5 ปีที่แล้ว +2

    effective video

  • @evaafif7239
    @evaafif7239 4 ปีที่แล้ว

    Can I ask a question :
    As we know the Anthrax toxins consist from 3 main proteins, One of them is Edema Factor ( EF) which is adenylate cyclase class2 , the genes for this class are known as cyaA, one of which is anthrax toxin.
    This cyaA is Bifunctional hemolysin/ adenylate cyclase, after cleaved in two proteins a calmodulin-sensitive adenylate cyclase (cyaA-ACD) and hemolysin , and this hemolysin is responsible for hemolysis of RBCs ,
    So finally How B.anthrax does not produce hemolysis on blood agar in laboratory diagnosis?

  • @gabriellevu5393
    @gabriellevu5393 5 ปีที่แล้ว

    Regarding the procedure for the antibiotic susceptibility test, would it still work if a spread plate technique was done opposed to using a swap to cover the HE plate?

  • @sunheepark9652
    @sunheepark9652 2 ปีที่แล้ว +1

    nice. thank you!!

  • @Mona-vj5nm
    @Mona-vj5nm 4 ปีที่แล้ว

    Hello! Is it possible to adapt this method to see the effect of visible light (blue, red, ..) on bacterial growth/inhibition? If not, which other method can be utilised? Thankyou!

    • @BiologyProfessor
      @BiologyProfessor  4 ปีที่แล้ว

      I would put the bacteria on different plates and expose each plate to one wavelength of light. You could then count the number of colonies growing after exposure to different light. Or you could grow bacteria in tubes of broth exposed to different wavelengths of light and then measure growth in the tubes using a spectrophotometer.

  • @gker21
    @gker21 5 ปีที่แล้ว +15

    Where it says very ineffective should have been very effective. spelling error I guess

    • @BiologyProfessor
      @BiologyProfessor  5 ปีที่แล้ว +8

      Oops! Yes, you are right. It should be "very effective".

  • @YoutubePremium-bq9ut
    @YoutubePremium-bq9ut 4 ปีที่แล้ว

    Can u please upload the video about microbial assay of streptomycin by cup plate method

    • @BiologyProfessor
      @BiologyProfessor  4 ปีที่แล้ว +1

      I have added it to my list. Thanks for the suggestion

  • @nikhilsenthil4500
    @nikhilsenthil4500 4 ปีที่แล้ว +2

    Can nutrient agar be used in this test?

    • @BiologyProfessor
      @BiologyProfessor  3 ปีที่แล้ว +1

      I’ve used LB and nutrient agar with my students. We call it a “modified disc diffusion assay” since it is not the standard protocol.

  • @sachinkondguli8250
    @sachinkondguli8250 ปีที่แล้ว

    Tq mam

  • @spacecomma4678
    @spacecomma4678 4 ปีที่แล้ว

    Hi, is there a std method of testing effectiveness of a biostatic agent applied to a surface? I’ve synthesized several colloidal copper hydrosols (suspended copper nanoparticles) to see if spraying on PPE will increase its antimicrobial properties. I was thinking of spraying discs and placing them in a lawn culture, but the nanoparticles are not intended to diffuse through the agar but rather stay attached to the material (discs). Is there a simple “zone of inhibition” type test, or can I just spray the hydrosol directly on part of the agar and look for growth? Heeeeeeelp!

    • @BiologyProfessor
      @BiologyProfessor  3 ปีที่แล้ว

      You could use paper discs dipped in your hydrosols to “touch” inoculated agar, then remove, leaving a layer of hydrosol behind. Do it at various concentrations and see if bacteria are inhibited. Can you just culture bacteria in 96-well plates with varying concentrations for hydrosols?

    • @spacecomma4678
      @spacecomma4678 3 ปีที่แล้ว

      Biology Professor thanks for you reply. Here’s a quick synopsis of what I’ve done: th-cam.com/video/3TWMc3K_PAg/w-d-xo.html (sorry 4 the bad jokes in advance). I’d like to verify whether copper treated fabrics inhibit microbial growth and for how long. Unfortunately I only understand the chemistry part. Any direction would be greatly appreciated.

  • @carriefinneran3964
    @carriefinneran3964 2 ปีที่แล้ว +1

    I think she was supposed to put very effective not very ineffective on the large shadow/zone

  • @1313babinski
    @1313babinski 3 หลายเดือนก่อน

    Can u do more this is such plain language my book said impregnated paper disk. Why do they need to complicate learning. Dumb it down then build it up.

  • @ivanbliminse4325
    @ivanbliminse4325 3 ปีที่แล้ว

    Is this method also called cup plate or is that something different?

    • @BiologyProfessor
      @BiologyProfessor  2 ปีที่แล้ว

      They are similar, but not quite the same. Longer explanation here: labmonk.com/microbiological-assay-of-antibiotics-using-cup-plate-method#:~:text=In%20the%20cup%20plate%20method,measured%20around%20the%20antibiotic%20disc.

  • @A.A.S231
    @A.A.S231 5 ปีที่แล้ว +1

    Thanks young pretty teacher!

  • @manarsmaan2624
    @manarsmaan2624 5 ปีที่แล้ว +1

    Is it 37 c or 35 c

    • @BiologyProfessor
      @BiologyProfessor  5 ปีที่แล้ว +2

      Usually 37C, but sometimes even 30C. Here is a link to multiple examples from the American Society for Microbiology and sometimes different people use different temperatures - www.asm.org/index.php/kirby-bauer

    • @manarsmaan2624
      @manarsmaan2624 5 ปีที่แล้ว +2

      @@BiologyProfessor Thank you Professor.

  • @iswaranand7387
    @iswaranand7387 5 ปีที่แล้ว +8

    very effective is being written as ineffective.

    • @BiologyProfessor
      @BiologyProfessor  5 ปีที่แล้ว +6

      Whoops! You are right. I say it correctly, but it is written incorrectly.

  • @ManutheJKboy
    @ManutheJKboy 5 ปีที่แล้ว +1

    🤗

  • @KazEditz99
    @KazEditz99 3 ปีที่แล้ว

    Oh my gaad..you're eyes are so pretty and menacing at the same time...Is this love at first sight❤😍..Thanks for the i formation tho.😊

  • @josephngulube4006
    @josephngulube4006 3 ปีที่แล้ว

    I am coming across the reporting of 0 mm diameter for the first time. I know of reporting
    6 mm, which is the diameter of the disc.

    • @BiologyProfessor
      @BiologyProfessor  3 ปีที่แล้ว

      See #7 on page 16 of the American Society for Microbiology’s protocol: asm.org/getattachment/2594ce26-bd44-47f6-8287-0657aa9185ad/Kirby-Bauer-Disk-DiffusionSusceptibility-Test-Protocol-pdf.pdf
      I know it’s counterintuitive, but recording 0 mM for no zone of inhibition is pretty standard. It’s the only thing I’ve ever seen.

  • @lybomanolov5054
    @lybomanolov5054 5 ปีที่แล้ว +4

    This can't be right...
    aren't smart girls supposed to be ugly?

    • @camerontaylor7471
      @camerontaylor7471 4 ปีที่แล้ว +2

      Lybo Manolov the more you use stereo types to navigate life ... the deeper you fall down the rabbits hole...

    • @emerotnica
      @emerotnica 4 ปีที่แล้ว +2

      you see the world with a key hole