Thanks for the video! May I know is there ant reason for using 2 cotton stick to swab 2 directions ? Can I use the same stick for both direction. And, do you know what type of bacterial is on the plate? I often saw similar bacterial colony on my agar plate but I am not sure what type of bacterial it is.
Yes. You can use same stick. Type of bacteria depends what we used for the broth culture preparation. We must know the bacteria name and identity. If you work with unknown bacteria then you could know by the bacterial identification tests (biochemical, immunological and molecular tests).
What is procedure if swabbed area is not possible to measure...in food industry many areas where is not possible to measure by using template like tubes, bends etc....at this situation what we can do...
Culture suspensions are prepared in saline solution because 0.85 % sodium chloride solution is isotonic in nature. Normal saline solution is routinely used as a diluent to adjust the turbidity of bacterial cell suspensions to help maintain cell integrity and viability.
@@MicroChemsExperimentsNo sir i don't have any equipments. Iam working at soap industry . so that iam asking how to check talcum powder surface using chemicals titaration method or any other options
Hello, what to do if the surface i will swab is in the gym equipments. Like how many hrs it will take for the swabbed samples to tranpsort in the laboratory? Fyi i am the researcher
Culture suspensions are prepared in saline solution because 0.85 % sodium chloride solution is isotonic in nature. Normal saline solution is routinely used as a diluent to adjust the turbidity of bacterial cell suspensions to help maintain cell integrity and viability.
Thank you so much for your videos.
They're so practical, easy to understand and applicable in the real world.
God bless you.
Thank you.
thanks a lot. all these videos were very helpful.🥰🥰
Hi can you show how to do and intepret/read contact plates for environmental surface sampling? Thanks
I love you for your work ❤❤
Thank you. Stay with us
Is that right (9x10x10)/100cm2
Cause you have diluted twice
What is the reference you are following the steps?
Thanks for the video! May I know is there ant reason for using 2 cotton stick to swab 2 directions ? Can I use the same stick for both direction. And, do you know what type of bacterial is on the plate? I often saw similar bacterial colony on my agar plate but I am not sure what type of bacterial it is.
Yes. You can use same stick.
Type of bacteria depends what we used for the broth culture preparation. We must know the bacteria name and identity. If you work with unknown bacteria then you could know by the bacterial identification tests (biochemical, immunological and molecular tests).
Please make a video on detection of campylobacter
Thanks sir please share more video microbiological testing releted food product dairy
Plz make an video on salmonella, shegilla and s. aureus species testing by swab method
Noted.
can we use peptone water instead of saline water
Is this formula not calulation correct?
Why use NACL in SPC agar medam
Base on which u done in this procedure?
Hi, may I know what material did you use to make the frame?
Can I use macconkey agar?
Do you have any article that supports this formula?
Please find from journal websites
Could you please refer any article using same formula? @@MicroChemsExperiments
Thanks
Please, create a video on Shigella species detection.
Noted
What is procedure if swabbed area is not possible to measure...in food industry many areas where is not possible to measure by using template like tubes, bends etc....at this situation what we can do...
Swab the whole surface and calculate using the surface name, as CFU/tube, CFU/bends
For hand swab same procedure shld followed??
Yes
Thanks you
You are welcome
Based on which standard u have done this?
FDA
Can we use PBS buffer for swab test?
Yes. You can use
Instead of TSA can I use plate count agar
You can
9×10×0.1=9 /100= 0.09 right
Can you explain ur calculation
Sorry, we can't explain here. We will develop a video later for chemical reactions and calculations. Stay with us.
Can i get reference of this method please
Environmental Monitoring, FDA, 2004
mam, can you create video about how to autoclaf your media ?
Noted
@@MicroChemsExperiments i can't wait this video 🤭
@@assyifa5033 soon
Why saline solution is used
Culture suspensions are prepared in saline solution because 0.85 % sodium chloride solution is isotonic in nature. Normal saline solution is routinely used as a diluent to adjust the turbidity of bacterial cell suspensions to help maintain cell integrity and viability.
Can I use buffered peptone water?@@MicroChemsExperiments
How to check talcum powder surface?
You need different mechanical device or instrument
@@MicroChemsExperimentsNo sir i don't have any equipments. Iam working at soap industry . so that iam asking how to check talcum powder surface using chemicals titaration method or any other options
Hello, what to do if the surface i will swab is in the gym equipments. Like how many hrs it will take for the swabbed samples to tranpsort in the laboratory? Fyi i am the researcher
Please write us in details at mail.mic.chem@gmail.com
Why sodium chloride is used ?
Culture suspensions are prepared in saline solution because 0.85 % sodium chloride solution is isotonic in nature. Normal saline solution is routinely used as a diluent to adjust the turbidity of bacterial cell suspensions to help maintain cell integrity and viability.
Thank you for the clarification ♥️♥️
@@wesleychebii5684 you are most welcome
9×10×0.1 =9 /100= 0.09
can u explain this calculation
please describe the reference document.
This is a FDA method
Can you told us where can find the method. I can find it. Please cited..@@MicroChemsExperiments
@@MicroChemsExperimentswhat number in FDA
mam, can you create video about how to make media Nutrien broth or others,please
Noted
@@MicroChemsExperiments i Will wait this video
I am Microbiologist help full video
It's error in calculation
How 9×10×0.1 =
How could plastic vials survived in 121 degree temp. 😮
Those plastics are autoclavable in quality to survive 121 degree Celsius moist heat
iam microbiologist please pharama company microbiology test videos
Wrong calculation formula, 0 .1 will be on denominator, Rest video is good.,
Superb. You are right. We did mistake here. Sorry
Hi, so may I know what is the correct equation and right answer?
@@Bavanigirl90 N= nV1/AV2
The same question ❓
Aluminium foils is not used for sterilisation it block steams penetration kindly read before making video
It can be used as a second barrier but not for primarily wrap