Touchdown PCR

Touchdown PCR - This lecture explains about the mechanism and principle of touchdown PCR reaction and the application of touchdown PCR in amplifying DNA fragments. Touchdown PCR is done to eliminate the formation of unwanted DNA amplification and it increases the specificity of the PCR reaction process. In touchdown PCR, we start with a very high annealing temperature which is close to the melting temperature of the primer and slowly reduce the temperature as PCR cycles are conducted. By this manner unwanted primer binding can be inhibited and specific PCR amplification of the target gene can be achieved.
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Thank you for watching the touchdown PCR lecture

[@amiraakilah8950]

all you said is correct unless one point is the definition of the melting temperature , you said it means the temperature at which the primer get damaged . however in PCR there is a beginning temperature of 95 degree to separate the 2 stands of DNA which is very high and do not damage the primer . so the correct defenition of the melting temperature is that it is the temperature that nearly below it the primer can bind specifically to the DNA strand and if we go far away below it , the primer bind unspecifically ti unwanted regions of the DNA.
thanks for the lecture , i like your work alot anyway

[@mominatayyab3659]

No , but the melting temp of primer is where primers get deattached from the strand i guess

[@yajurarora8285]

in google and various other sites, it is mentioned that the annealing temp at starting is 5-10 degrees higher than tm not lower. You had used the wrong term IG

[@MOHITKWATRA]

Very nice Shomu

[@shomusbiologyofficial]

Thank you

[@Farhab]

Awesome

[@shomusbiologyofficial]

Thank you

[@swapnilbaraskar6367]

Helpful!!!
Thanks a lot..

[@shomusbiologyofficial]

You're welcome

[@marianamarin8493]

TD-PCR purpose is to figure out which is the best annealing temperature?, and if that is correct, how do you know at which temperature the primers binded correctly?

[@kubraaslan4693]

thanks for the clear expressions. It was also very helpful to understand what exactly the critical point of running PCR and was inspiring for the trouble shooting of my Multiplex.

[@fatehmohammad7191]

Thank you sir well explained

[@shomusbiologyofficial]

You're welcome

[@AnkushSharma-zv5hv]

very well explained

[@soniamomnougui6032]

Thankss sooo much, it's so clear

[@ronaklashkari4124]

Good explanation

[@shomusbiologyofficial]

Thank you

[@harikeshyadav1011]

Sir nested PCR best hai ya rt PCR

[@shomusbiologyofficial]

Depending upon use

[@harikeshyadav1011]

@@shomusbiologyofficial HIV test me rt PCR best hai ya nested pcr

[@___yuuki6060]

thank you so much !!

[@shomusbiologyofficial]

You're welcome

[@belladas1475]

Thank you sir

[@shomusbiologyofficial]

You're welcome

[@ImranAhmad-df3mi]

Thanks a lot sir

[@shomusbiologyofficial]

You're welcome

[@johnnysong9781]

How can I get access to the privatized video clips?

[@swatichaudhury9933]

Thank you 🙂 sir....

[@shomusbiologyofficial]

You're welcome

[@hafsasaiyed5067]

Which type of primers are used in this process ?

[@Farhab]

No specific primers for this type

[@Ganesh-lt8km]

Mastt❤❤

[@shomusbiologyofficial]

Thank you

[@Mumma0712]

Can you explain about single cell pcr

[@shomusbiologyofficial]

Okay

[@jonaidahmadmalik8035]

Nice

[@shomusbiologyofficial]

You're welcome

[@anud6920]

dada tumi kono college e poraccho?

[@meerpeya5139]

Not so good we expect alot not limited from u

[@bavadharaniramalingam3771]

Plz teach very clearly
Ur voice are not clear

[@bavadharaniramalingam3771]

Avoid unnecessary explanation

[@triptyayesha8845]

Very well explained

[@shomusbiologyofficial]

You're welcome