Pyrosequencing
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- เผยแพร่เมื่อ 26 มิ.ย. 2024
- This lecture explains about the pyrosequencing technique. This is a type of next generation sequencing technology that explains the high throughput DNA sequencing method to know the DNA sequence. This lecture explains pyrosequencing steps in details.
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Hi, I am a great fan of yours as you are doing an awesome job and your videos really help me a lot. But still I have to make this comment so that a misunderstanding does not take place. In the video at 8:28 you said by mistake that ATP converts luciferin into luciferase which is not true. Rather ATP converts luciferin into oxyluciferin that gives luminescence in presence of luciferase enzyme. I know you must have a clear idea about that and you probably had a slip of tongue (because later you only said from luciferin, light is produced with the help of luciferase). So, if you could kindly mention the correct information in a pinned comment or in the description then I think no further confusion will take place. Cheers! And keep teaching us!
precisely!!
Thanku sir
Another mistake APS is Adenosine phosphosulfurase
@@MuhammadIslamag adenosine phosphosulfate, as it is a substrate and the enzyme that converts these ppi and substrate into ATP is ATP sulfurylase.
Thank you
Thumbs up to the instructor. Keep the good work. Bardhan, I appreciate and commend your mannerism of correction. Those are the values proper education instills in our minds.
I offer my salutations to all .. One of your kind from Nigeria
By far the most simplest video of pyrosequencing I've possibly ever seen. You make us students understand such complicated topics with such accuracy and ease. Thankyou so much. Keep Uploading Content Sir. You are legit a blessing to the science community
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When you said "if there is no thymine, then adenine will not attach and light will not be produced" I almost fell in love with you, you've saved my day. I am forever grateful. Keep doing your amazing work and congrats for the studying you have done in order to reach this level of understanding.
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Watching this video 4 years later in 2020 is a bliss. Finally was able to grasp the idea of Pyrosequencing in probably the simplest way possible.
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You are a legend, explain everything so clearly! Thank you so much, you are saving me in my exam!
We are blessed to attend such amazing lectures and clarify our concepts 😃👍
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Thank you so much bro. I live in Korea and I am a high school student studying to get admission to Seoul Medical School. Your lectures and tutorials are so helpful bro. Take care man, take care.
Really useful and simple explanations. Thank you and please keep uploading content! Greetings from Costa Rica
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APS is adenosine 5' phosphosulfate, not ammonium persulphate.
@@sinthy8447. does pointing out the mistake of someone is bad????
thank u brother
@@sinthy8447 What is wrong in pointing out mistakes. What is even wrong in making mistakes! It is just human error.
@@shubhagatasarkar exactly!
@@sinthy8447 chup
very clear explanation. thank you very much
Thank you!!! You are the only one who was able to explain this to me!
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You tha man Shomu. Thanks for helping out.
+H2OGOAu you're welcome
Thanku sir for helping me
Appreciate much for the clear and interesting explanation for pyrosequencing.Its helps more on my understanding!
+tai huili thank you. Glad it helped
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Thank you very much .It is very clear explanation.
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Nice explanation sir!
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Very nice and well explained lecture
Amazing explanation.. finally I get it!
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perfect ... thank you sooooo much
Sir ,when u say if u like the video then click the like option then i think that how anyone cannot like your teaching video. If i forget to click the like option then it does nt mean at all that i dont like.
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I understanded alot of topics from your videos, big thank you Dr.Shomu's.
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thank you very much, it is simple and perfect.
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THANKYOU so much youhave helped me to understand prosequencing very well
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@@shomusbiologyofficial you have no idea but u have been helping me a lot with my exams. The clear teaching with daigramatic representations.. God bless. I'm. Sure a lot feel the same too.
Dear sir, thank very much for your nice and informative lectures, we have a request if you could provide a lecture about RAD sequencing. it will be your kindness.
very useful ... thank you sir :)
hello. really liked thie video. can you please make such a video on Support oligo ligation and detection(SoLiD) technique too? that will be great.
well done May Allah ease every thing for you as you are doing for me
I AM A DOCTOR ….I LOVE YOUR VDOS….KEEP GROWING……GOD BLESS YOU
Thank you doctor.
thanks for that wonderful explaination
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I really like it bro. make a video about shotgun and clone contig sequencing
these lectures never disappoint..🎉😊
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explanation is awesome
+saranya krishnan thank you.
Thank you so much for your explanation it's really clear and it helps me a lot
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thank you brother.....
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this was helpfull Sir
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Thank you so much! I spent so much time trying to understand this from my lecture notes.
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@@shomusbiologyofficial thank you sir...plz teach us always like that I am having my Icar net exam in june I am following just ur videos lectures😁😁 only they r best!! For prep
Excellent video...helped me a lot
Thankyou so much sir 🙏😊 great explanation
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Really good teaching holding you have sir I can understand difficult concepts also thank you sir.
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best and the easiest I've seen so far
Thank you
Thank you for the lecture🤯🤯👏👏
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very convoluted lecture
Thank u soo much sir. It means a lot...
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Lovely!
This is very nice. Great!
I wanted to request for targeted next gene sequencing. please make videos on this for better understanding, your explanation is too good. Thank you so much for making us to understand the pyrosequencing.
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Brilliant ❤️
This is amazing!!! One question, how can we know if it’s a heterozygous?
thanks sir, helps a lot
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thnk u sir...
Thank you!❤
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Shomu da very informative video. There is one slight mistake : APS is adenosine 5' phosphosulphate. I have one doubt what is the use of adenosine alpha thiophosphate ?
Btw great video
Ur follower from KOLKATA ♥️♥️
thanks you very much!!!! can u explain why do we need an adaptor please ?
much useful .. thankyou
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Really love you Dada...
Thank you
Thks sir
please make video on genome finishing - gaps and their resolution
would be very helpful! ❤
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Very well explained.
I have query : In a well we have multiple fragments (having varied sequence), then how can we detect the sequencing because at the same time nucleotides are added at the multiple fragments ?
awesome
Thank you dada.....
APS doesnt stands for ammonium per sulphate but it stands for adenosine phospho sulphate. still your lectures are really helpful
Welldone sir....
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Good explaination
Thank you
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+Gurkamal kaur thank you. Glad you liked my lectures
thank u sir.
+Shilpa Subash you're welcome
Very well explained ! Only one thing .... APS is Adenosine 5' phosphosulfate !
Thanks, man 👍.
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Very well explained👌
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Well explained my dear !
+KOUA Patrick thank you very much
Thank you sir
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You are doing great job bro. APS is Adenosine phosphosulfurase
Yes it is
Sir this type of video gives more understanding rather than animated one...
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Our professor should learn from you
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@Shomu da, apni na thakle exam gulo te udhhar hote partam na kokhonoi! Happy Teacher's Day, Sir! Bhalo thakben! :)
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