Northern blotting technique

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  • เผยแพร่เมื่อ 24 ธ.ค. 2024

ความคิดเห็น • 274

  • @cindrellaray7197
    @cindrellaray7197 10 หลายเดือนก่อน +6

    A teacher like you is a blessing of God. The way you break down such complex theories which are so boring to read from books and manuals into this simple way it feels like a story telling. Thank you Sir for this genuine effort. Your dedication and excellent way of teaching has changed the lives of millions of students for the better.

    • @shomusbiologyofficial
      @shomusbiologyofficial  10 หลายเดือนก่อน +2

      Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures. Please stay tuned for more

  • @FootyShorts510
    @FootyShorts510 3 ปีที่แล้ว +31

    This guy is the goat. Very informative and easily digestible material, keep up the great videos!

  • @nidhinmangalasseri4061
    @nidhinmangalasseri4061 7 ปีที่แล้ว +110

    Im a biotechnology student......if i got a good mark in the exam its only because of you..... thanks.... All the best

    • @mercypeach9919
      @mercypeach9919 3 ปีที่แล้ว +2

      I am a biotechnology student too, but I love his explanations I really understand. I am in Uganda but I want to work from USA how can I get a chance?

    • @iflafayaz576
      @iflafayaz576 ปีที่แล้ว

      Thank you 😘having exam in biotechnology on 22🙏😘

  • @Emma-dm2cd
    @Emma-dm2cd 5 ปีที่แล้ว +27

    Hi Shomu! I've been watching your videos for a few years now, and never really took the time to express my gratitude. Every video furnishes a detailled and clear explanation, with a calm and slow voice. I really enjoy it every time, and always am less ignorant when leaving your channel. Thanks again, I wish you all the best.

  • @swatisucharita7557
    @swatisucharita7557 5 ปีที่แล้ว +27

    Best Bio teacher ever in my life. thank you sir😇

  • @lexfilyk4704
    @lexfilyk4704 5 ปีที่แล้ว +39

    this was actually way better than the way my prof explained it.

  • @tig_ress
    @tig_ress 3 ปีที่แล้ว +3

    yaaaaaaaaaar someone tell this guy i fell in love with him

  • @falgunigurjar5479
    @falgunigurjar5479 ปีที่แล้ว +4

    For 4 years of my btech journey i paid to university but got to understand topics only because of sir.
    Each and every topic of biotechnology is explained in such a detail that we just need to see his video and ur prepration is done.
    One of the best teacher 💯

  • @yashashreejoshi1050
    @yashashreejoshi1050 4 ปีที่แล้ว +12

    Sir I'm a bioengineering student nd we ve genetic engineering molecular biology nd genetics all these subjects i have studied through your coaching sir nd had got vevry good marks thank u so much sir God bless u a lot u are a true passionate teacher

  • @timotejjadud494
    @timotejjadud494 7 ปีที่แล้ว +2

    absolutely fantastic..Southern blotting or Northern blotting, all clear now. Very gifted man, wish you all the best!

  • @lyanadhamirah7381
    @lyanadhamirah7381 5 ปีที่แล้ว +6

    thank you so much sir, for deciding to become a youtube tutor. all your videos help me so much!!

  • @Nayl.Photography
    @Nayl.Photography หลายเดือนก่อน +1

    i did not understand even by watching 3d animation of the technique but by watching this video now i understood the whole process thankyou for existing

    • @shomusbiologyofficial
      @shomusbiologyofficial  หลายเดือนก่อน

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @Kysersozé1
    @Kysersozé1 4 ปีที่แล้ว +8

    You are absolutely brilliant. Thank you for your help.

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @dikshagaunkerd.5973
    @dikshagaunkerd.5973 8 ปีที่แล้ว +5

    Sir, I am having my presentation on Blotting techniques. After watching this video (northern blotting, southern blotting and weatern blotting techniques) i am very confident that i will defenatly do better and get good marks.
    Thank you.

    • @shomusbiologyofficial
      @shomusbiologyofficial  8 ปีที่แล้ว

      +Gavker Dixa thank you. It's nice to hear that you are getting benefit from the videos

  • @aliraza-hc4nw
    @aliraza-hc4nw 6 ปีที่แล้ว +4

    bhai im from pakistan ur method off teaching isssss greaaat keep ittt up

  • @subhadipghosh9227
    @subhadipghosh9227 3 ปีที่แล้ว

    Someone is there to teach you so easily.

  • @KS1998-e4s
    @KS1998-e4s 4 ปีที่แล้ว +5

    Hyy,... I am Be-tech Biotechnology student of 2nd year..... Jst want to tell you that u became a inspiration to all the biology students and also to the Teachers of science section across the world..... thank u. so.. much for helping us.... and not going frustrated during this lockdown... Teachers has started sending the notes and also they will be conducting the test next week......... without any explaination to students they jst provided a book pdf which will not help the students....... and here u came as a Saviour for the students..... thnk u so...much... now i m nt frustrated.......

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว +1

      Glad to hear that you're getting benefit from my lectures. Please subscribe and share

  • @sugandhajha7740
    @sugandhajha7740 6 ปีที่แล้ว +5

    Before exams I assure to watch all your videos from my syllabus . 🙏

  • @hodannuuh1254
    @hodannuuh1254 5 ปีที่แล้ว +5

    He explained it very well , thanks a lot

  • @PriyaSingh-iz2qc
    @PriyaSingh-iz2qc 5 ปีที่แล้ว +1

    U explained it in easiest way... 🙂🙂🙂

  • @rajlaxmiprasad7087
    @rajlaxmiprasad7087 4 ปีที่แล้ว +5

    Watched many times.helps lot.। Thank U sir

  • @sehar573
    @sehar573 11 หลายเดือนก่อน +1

    Hi brother your method of delivering lecture really make me to fall for study 😁lots of love brother

  • @hayakhan1211
    @hayakhan1211 8 ปีที่แล้ว +8

    thank u so much shomu. ur videos are a gem. i mastered blotting concept which i didnt even understood in med school. i wish u also had explained northwestern blotting. if u can then please also upload that

  • @risharajkhowa5060
    @risharajkhowa5060 ปีที่แล้ว +1

    Sir ,you r just amazing at your teaching skill

  • @obeddosty2938
    @obeddosty2938 2 ปีที่แล้ว +1

    You're born to teach

  • @madhuparnabannerjee8180
    @madhuparnabannerjee8180 5 ปีที่แล้ว

    My niper exams are in June and if I can make it through with biotechnology then all thanks to you coz I don't have to read books I just watch your videos and understand the concept. Thank you

  • @velushetty6709
    @velushetty6709 2 ปีที่แล้ว +2

    This video was very useful to my exam
    Thank you❤

  • @dipaliburle5733
    @dipaliburle5733 4 ปีที่แล้ว +2

    Nice shomus, u have very good commamd on all terms of life science, nice explanation

  • @physiologyfun1689
    @physiologyfun1689 3 ปีที่แล้ว

    Its amazing. ....huge respect from Pakistan

  • @Heyitsanuradha
    @Heyitsanuradha 3 ปีที่แล้ว

    I'm biotechnology student.. nd to be honest you teach much much better than my faculty.

  • @sudipnale7664
    @sudipnale7664 4 ปีที่แล้ว +1

    Sir your videos are really helpful for me.

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @lahariramakrishna1868
    @lahariramakrishna1868 4 ปีที่แล้ว +2

    its really gud that u reply most of the ppl dropping a comment ,anyways thank u

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @mehulstar96
    @mehulstar96 8 ปีที่แล้ว +1

    Thank you so much!!! All your videos are so helpful!

  • @shwetagupta9004
    @shwetagupta9004 5 ปีที่แล้ว +1

    You are genius suman sir .

  • @sabasafura
    @sabasafura 7 ปีที่แล้ว

    thank u...I wrote dis technique in my exam today...I was easily writing it...

  • @hurmajaved4869
    @hurmajaved4869 8 ปีที่แล้ว

    thanks alot sir ur videos are really helpful people all over the world are getting benefits from them including me.....i am from pakistan .GOD bless u sir

  • @vyghavinod2809
    @vyghavinod2809 2 ปีที่แล้ว +1

    Thank u sir..U r such a wonderful teacher

  • @starnancy6173
    @starnancy6173 5 ปีที่แล้ว +1

    Hats off to your effort..Keep it up..

  • @rohitasgaonkar8479
    @rohitasgaonkar8479 5 ปีที่แล้ว

    I was confused in northern blotting thanks sir for explaining so precisely

  • @mayukhroy5393
    @mayukhroy5393 4 ปีที่แล้ว +1

    Your Video was too helpful, Sir.

  • @abithavarshinisivakumar7279
    @abithavarshinisivakumar7279 3 ปีที่แล้ว +3

    I'm a microbiology student ur lectures helping out a lot

  • @mamtakol9575
    @mamtakol9575 6 ปีที่แล้ว +1

    Thank you so much sir .ur videos are very useful for my study. U teach very well, i like so much ur ability to teach....

  • @akashnandy6802
    @akashnandy6802 6 ปีที่แล้ว

    Shomu da.... Tumi guru shera

  • @pradeepsandaruwangunawardh8811
    @pradeepsandaruwangunawardh8811 7 ปีที่แล้ว +3

    very perfect and simply explained.. and thank you very much.

  • @Zhi-u6j
    @Zhi-u6j 11 หลายเดือนก่อน +1

    Great explanation thanks ❤

  • @ritikasoni3490
    @ritikasoni3490 5 ปีที่แล้ว +1

    amazing lecture sir. you explained it so easily to make it understand for us. 👍👍👍. very nice

  • @amisharastogi9109
    @amisharastogi9109 3 ปีที่แล้ว +1

    Thankyou sir for such a nice explanation.

  • @anagantisandhya644
    @anagantisandhya644 4 ปีที่แล้ว +1

    Thank u sir for ur classes

  • @bassomalbarasy2214
    @bassomalbarasy2214 ปีที่แล้ว +1

    Thank you shomu 💗

  • @pascalineuwamariya605
    @pascalineuwamariya605 5 ปีที่แล้ว +1

    you did an amazing job explaining this!!!

  • @spmaheriya9935
    @spmaheriya9935 6 ปีที่แล้ว +2

    I'm your fan sir ! Awesome your all video's..👌

  • @sidrakhan8589
    @sidrakhan8589 5 ปีที่แล้ว

    Sir you are doing a great job. Keep up good work

  • @jorgeribeiro9591
    @jorgeribeiro9591 8 ปีที่แล้ว +3

    You're amazing dude. Thanks a lot

  • @aienchopz4648
    @aienchopz4648 5 ปีที่แล้ว

    Thanks bro..it helps alot..got this particular topic presentation on monday 😄

  • @amanialyaseer5129
    @amanialyaseer5129 6 ปีที่แล้ว

    Ur like the best on youtube u rock

  • @angellesmith7585
    @angellesmith7585 5 ปีที่แล้ว +2

    Amazing work!!! Thanks for sharing!!!

  • @fahmeedashaikh7793
    @fahmeedashaikh7793 3 ปีที่แล้ว +1

    You explained everything very well.
    Thank you❤️

  • @nyashagava9476
    @nyashagava9476 4 ปีที่แล้ว

    iwewe shomu mazivideo ako akarebesa aya shaaaa... apa unowanza ngano futi.

  • @nehabhardwaj3483
    @nehabhardwaj3483 3 ปีที่แล้ว +1

    Waaooo sir amazing thankuuu so much sir

  • @helanpeter2429
    @helanpeter2429 4 ปีที่แล้ว +1

    Sir, thankyou so much . It's so helpful.

  • @kainatikhlaq4221
    @kainatikhlaq4221 5 ปีที่แล้ว

    beautiful method of teaching....thank you sir

  • @bongskitchen649
    @bongskitchen649 4 ปีที่แล้ว +1

    thank sir very helpful video real i can understand each and very point

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @raibhangaikwad1818
    @raibhangaikwad1818 6 ปีที่แล้ว +1

    best videos sir . keep it up!

  • @johntindell9591
    @johntindell9591 3 ปีที่แล้ว +2

    Thank you so much.

  • @shrutikachawla2734
    @shrutikachawla2734 5 ปีที่แล้ว +1

    Amazing lectures ,Thankyou so much Sir

  • @chakkravarthyv8333
    @chakkravarthyv8333 2 ปีที่แล้ว +3

    Please teach something apart from the book

    • @shomusbiologyofficial
      @shomusbiologyofficial  2 ปีที่แล้ว

      That's called research. You need to do PhD for that

    • @chakkravarthyv8333
      @chakkravarthyv8333 2 ปีที่แล้ว +1

      @@shomusbiologyofficial Dear Mr. Shomu,
      I think you have mistaken me. Your way of explaining the fundamentals are good and I really appreciate your efforts. I am just a mechanical engineer but I have some few suggestions for you, hope you don't take it wrong. If you are teaching about western blotting you can take a real time example. You can start like For eg: If a patient is undergoing hip transplant or bone transplant surgery then how will you find that the wound is getting healed or not? Lets see in what way Western Blot technique is going to be helpful. There are particular bone proteins ( RunX2, GAPDH, RANKL, Col-1) which can promote the bone joining and there are other sets of inflammatory proteins (TNF Alpha, TNF Beta, Interleukin 1,2) that are responsible for bone cell apoptosis and necrosis. So we need to find which are the proteins are upregulated and which are the proteins are down regulated. If bone joining proteins are upregulated then it means the patients bone joining ability is good , if inflammatory proteins are upregulated then wound healing process is hindered. To know either proteins are upregulated or downregulated we need to perform western blot analysis. After saying these things you shall explain all the fundamentals then all students can learn from the application perspective. I have one more kind suggestion for you. In SDS page in western blot is based on the molecular weight of the protein. Please give some examples. ( For an Example : The molecular weight of Col-1 is 138kDA, GAPDH is 36kDA). When we observe SDS page we shall find is there any bands that are present close to 138kDA or not . If a band is present but very less intense then it means that protein reaction is less intense. If it is a dark band then it means that particular protein has been upregulated. If there is no band in SDS page corresponding to 138kDA then that particular protein is absent. Finally we shall quantify the band intensity using Image J software which is a universally accepted software and free software. Like wise if you take a real time problem and if you explain in this way then it will quickly reach everyone and it will be more beneficial. In our Indian education we have read matrices, integral calculus during our school days and college days. But still now we never used any. Because we know how to do integral calculus but we don't know where to use it. So always teach from application perspective. A budding teacher like you should reach great heights. Good luck.

  • @JyotiYadav-pz9ko
    @JyotiYadav-pz9ko 2 ปีที่แล้ว +1

    Sir u r amazing

  • @ahangerruqiya5882
    @ahangerruqiya5882 6 ปีที่แล้ว

    Thanks sir aap bohut aache se explain karte ho.God bless u sir.

  • @faizanalam3789
    @faizanalam3789 7 ปีที่แล้ว +3

    thank u so much its more helpful ,understand easily

  • @ujjwalchauhan.in.
    @ujjwalchauhan.in. 4 ปีที่แล้ว +1

    In 1977 , Northern Blotting technique was discovered not in 1979.
    Question . Is northern blotting used only for m-Rna ?

  • @afsajunaid3743
    @afsajunaid3743 5 ปีที่แล้ว +3

    what is the advantage of using aminobenzoxymethyl filter paper over nitrocellulose filter paper?

    • @abuzarkhan527
      @abuzarkhan527 4 ปีที่แล้ว

      Southern techniques has been of enormous value but it was thought that it cud not be applied directly to the blot transfer of rna separated by gel electrophoresis since rna was found not to bind to nitrocellulose. Therefore james alwine david kemp amd george stark devised a procedure in which rna band are blot transferred from the gel on to the chemically reactive paper where they are bound covalently. The reactive paper is prepared by diazotization of aminobenzyloxy methyl paper.

  • @femina129
    @femina129 2 ปีที่แล้ว +1

    Very impressive

  • @markeshawtiruneh9591
    @markeshawtiruneh9591 7 ปีที่แล้ว +2

    Thank u sir for your valuable videos

    • @shomusbiologyofficial
      @shomusbiologyofficial  7 ปีที่แล้ว +2

      +Markeshaw Tiruneh you're welcome

    • @markeshawtiruneh9591
      @markeshawtiruneh9591 7 ปีที่แล้ว

      thank u Dear I proud of you would you tell me the specifications for Real Time PCR, DNA sequencer, Gell ellectrophoresis, DNA extaction kit, and then laboratory design since we are going to establish molecular laboratory and I am from Ethiopia

  • @ameeshakushwaha9302
    @ameeshakushwaha9302 2 ปีที่แล้ว +1

    Thank you so much sir

  • @MSCstudent-gk1kz
    @MSCstudent-gk1kz 2 ปีที่แล้ว +1

    thanks my teacher

  • @tejasvisakhamuri1380
    @tejasvisakhamuri1380 4 ปีที่แล้ว +2

    Awesome!, thank you

  • @PriyaSingh-iz2qc
    @PriyaSingh-iz2qc 5 ปีที่แล้ว +1

    Thnq you.. 🙂🙂

  • @ssss...7896
    @ssss...7896 6 ปีที่แล้ว

    tq sr...its helpfull for my exam

  • @kalpanajaiswar5025
    @kalpanajaiswar5025 5 ปีที่แล้ว +1

    Thank you so much sir 🤗

  • @ishangarg5229
    @ishangarg5229 6 ปีที่แล้ว

    veere awesome ae yr tu....... 😃

  • @nidhinmangalasseri4061
    @nidhinmangalasseri4061 7 ปีที่แล้ว +1

    I have an question....that when we add dna probe how it hybridise with the targeted rna fragment... Bcz it consist of many uracil nitrogen bases... Can you please tell..... i understood all the blotting techniques all credits go to you

    • @nayanavinayan7985
      @nayanavinayan7985 5 ปีที่แล้ว +1

      Uracil can naturally pair with adenine through double hydrogen bonds just like thymine.thymine and uracil are similar expect that thymine has an additional methyl group

  • @esthersharon366
    @esthersharon366 6 ปีที่แล้ว

    Very helpful.. loved the video...

  • @gayatribhatt4869
    @gayatribhatt4869 5 ปีที่แล้ว +2

    Sir thanks a ton

  • @manpreetsandhu2483
    @manpreetsandhu2483 4 ปีที่แล้ว +2

    Thank u sir 😊

  • @jarintasneemjita733
    @jarintasneemjita733 3 ปีที่แล้ว

    ধন্যবাদ স্যার! 😍

  • @joelfernandez5141
    @joelfernandez5141 4 ปีที่แล้ว +1

    Sir how are probes prepared and how we would know the exact sequence of target DNA

    • @sangeetadevi5819
      @sangeetadevi5819 4 ปีที่แล้ว +2

      I also wanted to ask the same question...😅🤔

  • @mirudulae9396
    @mirudulae9396 5 ปีที่แล้ว +5

    why can't we use RNA probe?

    • @ujjwalchauhan.in.
      @ujjwalchauhan.in. 4 ปีที่แล้ว

      We can use , but rna probe is more prone to degradation while DNA probe is more stable.

  • @oinambobochandsingh89
    @oinambobochandsingh89 4 ปีที่แล้ว +1

    Wonderful

  • @anjacerovsek6637
    @anjacerovsek6637 5 ปีที่แล้ว +1

    The best! Thank you

  • @satabdideka31
    @satabdideka31 4 ปีที่แล้ว

    Sir you mentioned the blotting buffer. Can you please tell what does the buffer contain?

  • @charlesmendith2444
    @charlesmendith2444 4 ปีที่แล้ว +1

    Thank you

  • @raibhangaikwad1818
    @raibhangaikwad1818 6 ปีที่แล้ว +1

    sir I have a doubt , we already know the neucleotide sequence which we want to use in preparation of genetically modified organism , then why extract it from some other organisms genome by using so many techniques ? why not prepare that gene itself artificially ? can we prepare such genes ? if not then how we are able to prepare probes ?

  • @m.krithikashri6246
    @m.krithikashri6246 2 ปีที่แล้ว

    Transfer of RNA from genetics northern bloating I want the practical protocol?

  • @jasmint7822
    @jasmint7822 6 ปีที่แล้ว

    some times board is not clear ,,,but ur amazing

  • @Amruthasbiology
    @Amruthasbiology ปีที่แล้ว

    Sir I have a doubt that why don't we use restriction enzymes to make the complex mRNA to linear RNA

  • @niteshsharmasharma7626
    @niteshsharmasharma7626 6 ปีที่แล้ว +1

    Thank you sir😊👌✌

  • @abumohammed9659
    @abumohammed9659 3 ปีที่แล้ว +1

    WONDERFULL

  • @swetaxavier6290
    @swetaxavier6290 6 ปีที่แล้ว +1

    Is this technique similar to dot blot technique?

  • @manvipathak1111
    @manvipathak1111 3 ปีที่แล้ว

    Bhaiya ek question hai apne kha gel is fragile isliye hum usme probing nhi kr skte ,Phir bhaiya gel ke upar jb weight rakha paper towel rakhe to vo kyu nhi break huwa

    • @manvipathak1111
      @manvipathak1111 3 ปีที่แล้ว

      Also very good mentor you are.Thanks for all wonderful lectures.

  • @sumantsahoo75
    @sumantsahoo75 5 ปีที่แล้ว

    Sir that buffer solution is SSC(sodium saline citrate)