A teacher like you is a blessing of God. The way you break down such complex theories which are so boring to read from books and manuals into this simple way it feels like a story telling. Thank you Sir for this genuine effort. Your dedication and excellent way of teaching has changed the lives of millions of students for the better.
Hi Shomu! I've been watching your videos for a few years now, and never really took the time to express my gratitude. Every video furnishes a detailled and clear explanation, with a calm and slow voice. I really enjoy it every time, and always am less ignorant when leaving your channel. Thanks again, I wish you all the best.
For 4 years of my btech journey i paid to university but got to understand topics only because of sir. Each and every topic of biotechnology is explained in such a detail that we just need to see his video and ur prepration is done. One of the best teacher 💯
Sir I'm a bioengineering student nd we ve genetic engineering molecular biology nd genetics all these subjects i have studied through your coaching sir nd had got vevry good marks thank u so much sir God bless u a lot u are a true passionate teacher
i did not understand even by watching 3d animation of the technique but by watching this video now i understood the whole process thankyou for existing
Sir, I am having my presentation on Blotting techniques. After watching this video (northern blotting, southern blotting and weatern blotting techniques) i am very confident that i will defenatly do better and get good marks. Thank you.
Hyy,... I am Be-tech Biotechnology student of 2nd year..... Jst want to tell you that u became a inspiration to all the biology students and also to the Teachers of science section across the world..... thank u. so.. much for helping us.... and not going frustrated during this lockdown... Teachers has started sending the notes and also they will be conducting the test next week......... without any explaination to students they jst provided a book pdf which will not help the students....... and here u came as a Saviour for the students..... thnk u so...much... now i m nt frustrated.......
thank u so much shomu. ur videos are a gem. i mastered blotting concept which i didnt even understood in med school. i wish u also had explained northwestern blotting. if u can then please also upload that
My niper exams are in June and if I can make it through with biotechnology then all thanks to you coz I don't have to read books I just watch your videos and understand the concept. Thank you
thanks alot sir ur videos are really helpful people all over the world are getting benefits from them including me.....i am from pakistan .GOD bless u sir
@@shomusbiologyofficial Dear Mr. Shomu, I think you have mistaken me. Your way of explaining the fundamentals are good and I really appreciate your efforts. I am just a mechanical engineer but I have some few suggestions for you, hope you don't take it wrong. If you are teaching about western blotting you can take a real time example. You can start like For eg: If a patient is undergoing hip transplant or bone transplant surgery then how will you find that the wound is getting healed or not? Lets see in what way Western Blot technique is going to be helpful. There are particular bone proteins ( RunX2, GAPDH, RANKL, Col-1) which can promote the bone joining and there are other sets of inflammatory proteins (TNF Alpha, TNF Beta, Interleukin 1,2) that are responsible for bone cell apoptosis and necrosis. So we need to find which are the proteins are upregulated and which are the proteins are down regulated. If bone joining proteins are upregulated then it means the patients bone joining ability is good , if inflammatory proteins are upregulated then wound healing process is hindered. To know either proteins are upregulated or downregulated we need to perform western blot analysis. After saying these things you shall explain all the fundamentals then all students can learn from the application perspective. I have one more kind suggestion for you. In SDS page in western blot is based on the molecular weight of the protein. Please give some examples. ( For an Example : The molecular weight of Col-1 is 138kDA, GAPDH is 36kDA). When we observe SDS page we shall find is there any bands that are present close to 138kDA or not . If a band is present but very less intense then it means that protein reaction is less intense. If it is a dark band then it means that particular protein has been upregulated. If there is no band in SDS page corresponding to 138kDA then that particular protein is absent. Finally we shall quantify the band intensity using Image J software which is a universally accepted software and free software. Like wise if you take a real time problem and if you explain in this way then it will quickly reach everyone and it will be more beneficial. In our Indian education we have read matrices, integral calculus during our school days and college days. But still now we never used any. Because we know how to do integral calculus but we don't know where to use it. So always teach from application perspective. A budding teacher like you should reach great heights. Good luck.
Southern techniques has been of enormous value but it was thought that it cud not be applied directly to the blot transfer of rna separated by gel electrophoresis since rna was found not to bind to nitrocellulose. Therefore james alwine david kemp amd george stark devised a procedure in which rna band are blot transferred from the gel on to the chemically reactive paper where they are bound covalently. The reactive paper is prepared by diazotization of aminobenzyloxy methyl paper.
thank u Dear I proud of you would you tell me the specifications for Real Time PCR, DNA sequencer, Gell ellectrophoresis, DNA extaction kit, and then laboratory design since we are going to establish molecular laboratory and I am from Ethiopia
I have an question....that when we add dna probe how it hybridise with the targeted rna fragment... Bcz it consist of many uracil nitrogen bases... Can you please tell..... i understood all the blotting techniques all credits go to you
Uracil can naturally pair with adenine through double hydrogen bonds just like thymine.thymine and uracil are similar expect that thymine has an additional methyl group
sir I have a doubt , we already know the neucleotide sequence which we want to use in preparation of genetically modified organism , then why extract it from some other organisms genome by using so many techniques ? why not prepare that gene itself artificially ? can we prepare such genes ? if not then how we are able to prepare probes ?
Bhaiya ek question hai apne kha gel is fragile isliye hum usme probing nhi kr skte ,Phir bhaiya gel ke upar jb weight rakha paper towel rakhe to vo kyu nhi break huwa
A teacher like you is a blessing of God. The way you break down such complex theories which are so boring to read from books and manuals into this simple way it feels like a story telling. Thank you Sir for this genuine effort. Your dedication and excellent way of teaching has changed the lives of millions of students for the better.
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures. Please stay tuned for more
This guy is the goat. Very informative and easily digestible material, keep up the great videos!
Thank you so much for appreciating my efforts
Im a biotechnology student......if i got a good mark in the exam its only because of you..... thanks.... All the best
I am a biotechnology student too, but I love his explanations I really understand. I am in Uganda but I want to work from USA how can I get a chance?
Thank you 😘having exam in biotechnology on 22🙏😘
Hi Shomu! I've been watching your videos for a few years now, and never really took the time to express my gratitude. Every video furnishes a detailled and clear explanation, with a calm and slow voice. I really enjoy it every time, and always am less ignorant when leaving your channel. Thanks again, I wish you all the best.
&जीजी
Best Bio teacher ever in my life. thank you sir😇
Thank you so much for appreciating my efforts
this was actually way better than the way my prof explained it.
Glad to hear that you are getting benefit from the videos
yaaaaaaaaaar someone tell this guy i fell in love with him
For 4 years of my btech journey i paid to university but got to understand topics only because of sir.
Each and every topic of biotechnology is explained in such a detail that we just need to see his video and ur prepration is done.
One of the best teacher 💯
I am glad to help you out
Sir I'm a bioengineering student nd we ve genetic engineering molecular biology nd genetics all these subjects i have studied through your coaching sir nd had got vevry good marks thank u so much sir God bless u a lot u are a true passionate teacher
Thank you so much for appreciating my efforts
absolutely fantastic..Southern blotting or Northern blotting, all clear now. Very gifted man, wish you all the best!
thank you so much sir, for deciding to become a youtube tutor. all your videos help me so much!!
Glad to hear that you are getting benefit from the videos
i did not understand even by watching 3d animation of the technique but by watching this video now i understood the whole process thankyou for existing
You're welcome. Glad to hear that you're getting benefit from my lectures
You are absolutely brilliant. Thank you for your help.
You're welcome. Glad to hear that you're getting benefit from my lectures
Sir, I am having my presentation on Blotting techniques. After watching this video (northern blotting, southern blotting and weatern blotting techniques) i am very confident that i will defenatly do better and get good marks.
Thank you.
+Gavker Dixa thank you. It's nice to hear that you are getting benefit from the videos
bhai im from pakistan ur method off teaching isssss greaaat keep ittt up
Thank you. Glad you liked my lectures
Someone is there to teach you so easily.
Hyy,... I am Be-tech Biotechnology student of 2nd year..... Jst want to tell you that u became a inspiration to all the biology students and also to the Teachers of science section across the world..... thank u. so.. much for helping us.... and not going frustrated during this lockdown... Teachers has started sending the notes and also they will be conducting the test next week......... without any explaination to students they jst provided a book pdf which will not help the students....... and here u came as a Saviour for the students..... thnk u so...much... now i m nt frustrated.......
Glad to hear that you're getting benefit from my lectures. Please subscribe and share
Before exams I assure to watch all your videos from my syllabus . 🙏
Glad to hear that you
He explained it very well , thanks a lot
You're welcome
U explained it in easiest way... 🙂🙂🙂
Watched many times.helps lot.। Thank U sir
You're welcome
Hi brother your method of delivering lecture really make me to fall for study 😁lots of love brother
Thank you so much for appreciating my efforts
thank u so much shomu. ur videos are a gem. i mastered blotting concept which i didnt even understood in med school. i wish u also had explained northwestern blotting. if u can then please also upload that
+haya khan thank you. Glad you liked my lectures
Sir ,you r just amazing at your teaching skill
Thank you
You're born to teach
Thank you so much for appreciating my efforts
My niper exams are in June and if I can make it through with biotechnology then all thanks to you coz I don't have to read books I just watch your videos and understand the concept. Thank you
This video was very useful to my exam
Thank you❤
You're welcome
Nice shomus, u have very good commamd on all terms of life science, nice explanation
Thank you. Glad you liked my lectures
Its amazing. ....huge respect from Pakistan
I'm biotechnology student.. nd to be honest you teach much much better than my faculty.
Thank you so much for appreciating my efforts
Sir your videos are really helpful for me.
You're welcome. Glad to hear that you're getting benefit from my lectures
its really gud that u reply most of the ppl dropping a comment ,anyways thank u
You're welcome. Glad to hear that you're getting benefit from my lectures
Thank you so much!!! All your videos are so helpful!
+Mehul Patel thank you
You are genius suman sir .
Thank you so much
thank u...I wrote dis technique in my exam today...I was easily writing it...
thanks alot sir ur videos are really helpful people all over the world are getting benefits from them including me.....i am from pakistan .GOD bless u sir
Thank u sir..U r such a wonderful teacher
You're welcome. Glad you like it
Hats off to your effort..Keep it up..
Thank you. Glad you liked my lectures
I was confused in northern blotting thanks sir for explaining so precisely
Your Video was too helpful, Sir.
You're welcome
I'm a microbiology student ur lectures helping out a lot
Glad to hear that you're getting benefit from my lectures
Thank you so much sir .ur videos are very useful for my study. U teach very well, i like so much ur ability to teach....
Thank you. Glad you liked my lectures
Shomu da.... Tumi guru shera
very perfect and simply explained.. and thank you very much.
Great explanation thanks ❤
You're welcome
amazing lecture sir. you explained it so easily to make it understand for us. 👍👍👍. very nice
Thank you. Glad you liked my lectures
Thankyou sir for such a nice explanation.
You're welcome
Thank u sir for ur classes
You're welcome
Thank you shomu 💗
You're welcome
you did an amazing job explaining this!!!
I'm your fan sir ! Awesome your all video's..👌
Thank you.
Sir you are doing a great job. Keep up good work
You're amazing dude. Thanks a lot
+Jorge Ribeiro glad my lectures helped you out
Thanks bro..it helps alot..got this particular topic presentation on monday 😄
Ur like the best on youtube u rock
Amazing work!!! Thanks for sharing!!!
Glad to hear that you are getting benefit from the videos
You explained everything very well.
Thank you❤️
You're welcome
iwewe shomu mazivideo ako akarebesa aya shaaaa... apa unowanza ngano futi.
Waaooo sir amazing thankuuu so much sir
You're welcome
Sir, thankyou so much . It's so helpful.
You're welcome
beautiful method of teaching....thank you sir
You're welcome
thank sir very helpful video real i can understand each and very point
You're welcome. Glad to hear that you're getting benefit from my lectures
best videos sir . keep it up!
Thank you so much.
You're welcome
Amazing lectures ,Thankyou so much Sir
You're welcome
Please teach something apart from the book
That's called research. You need to do PhD for that
@@shomusbiologyofficial Dear Mr. Shomu,
I think you have mistaken me. Your way of explaining the fundamentals are good and I really appreciate your efforts. I am just a mechanical engineer but I have some few suggestions for you, hope you don't take it wrong. If you are teaching about western blotting you can take a real time example. You can start like For eg: If a patient is undergoing hip transplant or bone transplant surgery then how will you find that the wound is getting healed or not? Lets see in what way Western Blot technique is going to be helpful. There are particular bone proteins ( RunX2, GAPDH, RANKL, Col-1) which can promote the bone joining and there are other sets of inflammatory proteins (TNF Alpha, TNF Beta, Interleukin 1,2) that are responsible for bone cell apoptosis and necrosis. So we need to find which are the proteins are upregulated and which are the proteins are down regulated. If bone joining proteins are upregulated then it means the patients bone joining ability is good , if inflammatory proteins are upregulated then wound healing process is hindered. To know either proteins are upregulated or downregulated we need to perform western blot analysis. After saying these things you shall explain all the fundamentals then all students can learn from the application perspective. I have one more kind suggestion for you. In SDS page in western blot is based on the molecular weight of the protein. Please give some examples. ( For an Example : The molecular weight of Col-1 is 138kDA, GAPDH is 36kDA). When we observe SDS page we shall find is there any bands that are present close to 138kDA or not . If a band is present but very less intense then it means that protein reaction is less intense. If it is a dark band then it means that particular protein has been upregulated. If there is no band in SDS page corresponding to 138kDA then that particular protein is absent. Finally we shall quantify the band intensity using Image J software which is a universally accepted software and free software. Like wise if you take a real time problem and if you explain in this way then it will quickly reach everyone and it will be more beneficial. In our Indian education we have read matrices, integral calculus during our school days and college days. But still now we never used any. Because we know how to do integral calculus but we don't know where to use it. So always teach from application perspective. A budding teacher like you should reach great heights. Good luck.
Sir u r amazing
Thank you
Thanks sir aap bohut aache se explain karte ho.God bless u sir.
thank u so much its more helpful ,understand easily
+Faizan Alam you're welcome
In 1977 , Northern Blotting technique was discovered not in 1979.
Question . Is northern blotting used only for m-Rna ?
We need it for mRNA only
what is the advantage of using aminobenzoxymethyl filter paper over nitrocellulose filter paper?
Southern techniques has been of enormous value but it was thought that it cud not be applied directly to the blot transfer of rna separated by gel electrophoresis since rna was found not to bind to nitrocellulose. Therefore james alwine david kemp amd george stark devised a procedure in which rna band are blot transferred from the gel on to the chemically reactive paper where they are bound covalently. The reactive paper is prepared by diazotization of aminobenzyloxy methyl paper.
Very impressive
Thank you
Thank u sir for your valuable videos
+Markeshaw Tiruneh you're welcome
thank u Dear I proud of you would you tell me the specifications for Real Time PCR, DNA sequencer, Gell ellectrophoresis, DNA extaction kit, and then laboratory design since we are going to establish molecular laboratory and I am from Ethiopia
Thank you so much sir
You're welcome
thanks my teacher
You're welcome
Awesome!, thank you
You're welcome
Thnq you.. 🙂🙂
tq sr...its helpfull for my exam
Thank you so much sir 🤗
You're welcome
veere awesome ae yr tu....... 😃
I have an question....that when we add dna probe how it hybridise with the targeted rna fragment... Bcz it consist of many uracil nitrogen bases... Can you please tell..... i understood all the blotting techniques all credits go to you
Uracil can naturally pair with adenine through double hydrogen bonds just like thymine.thymine and uracil are similar expect that thymine has an additional methyl group
Very helpful.. loved the video...
Sir thanks a ton
You're welcome
Thank u sir 😊
You're welcome
ধন্যবাদ স্যার! 😍
Thank you
Sir how are probes prepared and how we would know the exact sequence of target DNA
I also wanted to ask the same question...😅🤔
why can't we use RNA probe?
We can use , but rna probe is more prone to degradation while DNA probe is more stable.
Wonderful
Thank you
The best! Thank you
You're welcome
Sir you mentioned the blotting buffer. Can you please tell what does the buffer contain?
Thank you
You're welcome
sir I have a doubt , we already know the neucleotide sequence which we want to use in preparation of genetically modified organism , then why extract it from some other organisms genome by using so many techniques ? why not prepare that gene itself artificially ? can we prepare such genes ? if not then how we are able to prepare probes ?
Same doubt,!!
Transfer of RNA from genetics northern bloating I want the practical protocol?
some times board is not clear ,,,but ur amazing
Sir I have a doubt that why don't we use restriction enzymes to make the complex mRNA to linear RNA
Thank you sir😊👌✌
You're welcome
WONDERFULL
Thank you
Is this technique similar to dot blot technique?
Bhaiya ek question hai apne kha gel is fragile isliye hum usme probing nhi kr skte ,Phir bhaiya gel ke upar jb weight rakha paper towel rakhe to vo kyu nhi break huwa
Also very good mentor you are.Thanks for all wonderful lectures.
Sir that buffer solution is SSC(sodium saline citrate)