Southern blotting

แชร์
ฝัง
  • เผยแพร่เมื่อ 18 พ.ย. 2024

ความคิดเห็น • 965

  • @nilutpaldas18
    @nilutpaldas18 ปีที่แล้ว +42

    Sometimes I think why don't we get professor like you in university?
    U r like a....... Complete this line friends

  • @shamimahmed-pk6ug
    @shamimahmed-pk6ug 5 ปีที่แล้ว +200

    I'm a student of biotechnology. I found that ur 100time's better than my prof. Thank you sir. May god bless you.

    • @shomusbiologyofficial
      @shomusbiologyofficial  5 ปีที่แล้ว +24

      Glad to hear that you are getting benefit from the videos. Please subscribe and share

    • @taslimkilledar9359
      @taslimkilledar9359 4 ปีที่แล้ว +5

      Exactly...I am also biotechnology student in msc..

    • @tanaya_ayarekar
      @tanaya_ayarekar 3 ปีที่แล้ว +1

      Exactly 💯

    • @saranshnigam3896
      @saranshnigam3896 3 ปีที่แล้ว +1

      I also

    • @forbii8778
      @forbii8778 ปีที่แล้ว +2

      ​@@taslimkilledar9359Bro is it worth doing msc in biotechnology??

  • @TheRealNG9
    @TheRealNG9 7 ปีที่แล้ว +90

    You are a machine my friend. I've watched a lot of videos about SB but a clear explanation of the principle of blotting was nowhere to find until I found your video. Thank you for helping me and you have my respect for what you do here. Sharing and explaining the information is an honorable thing to do. I am glad that there are such people as you.

  • @강아지-m5j
    @강아지-m5j 5 ปีที่แล้ว +70

    I LOVE your accent. I can easily understand because you point out all the important parts with accent. And also you know what kind of questions listeners would have and you answered all of them. It is like taking a private lesson. Thank you so much! From Korea :)

  • @BTSARMY-sb8sh
    @BTSARMY-sb8sh 2 ปีที่แล้ว +57

    SAYING "THANK U" WILL NEVER ACTUALLY THANK YOU SIR.
    YOU HAVE MADE THIS TOPIC CRYSTAL CLEAR TO ME BY YOUR AMAZING TEACHING STYLE. U. ARE. THE. BEST. I NEEDED U. YOU HELPED ME FOR THE PREPARATION OF MY PRESENTATION. U ARE AN ABSOLUTE GREAT TEACHER.I CAN NEVER THANK U ENOUGH EVER!!!
    LOTS OF LOVE & RESPECT FROM PAKISTAN 💜🌸 , FEELING BEYOND LUCKY TO FIND YOUR CHANNEL.💜😇 YOUR NEW SUBSCRIBER 👉💜👈

    • @shomusbiologyofficial
      @shomusbiologyofficial  2 ปีที่แล้ว +8

      Thank you so much for appreciating my efforts

    • @BTSARMY-sb8sh
      @BTSARMY-sb8sh 2 ปีที่แล้ว +2

      @@shomusbiologyofficial U are most welcome Sir 💜

  • @drbabaranwarkhan2360
    @drbabaranwarkhan2360 4 ปีที่แล้ว +36

    Very comprehensive.
    I am a doctor and you have very well explained about all blotting methods.Thank you

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว

      Glad to hear that you're getting benefit from my lectures

    • @normadicn5700
      @normadicn5700 4 ปีที่แล้ว

      Same here. He is so on point

    • @kathmandunepal9547
      @kathmandunepal9547 4 ปีที่แล้ว

      I think the lab technicians would know more about blotting than doctors? Because it's literally what they do everyday!

  • @chayanikagogoi640
    @chayanikagogoi640 3 ปีที่แล้ว +22

    Sir your biology lectures has saved me in my graduation and now they are still a blessing for me in my master's degree.🥺..thankyou so much sir

    • @shomusbiologyofficial
      @shomusbiologyofficial  3 ปีที่แล้ว +1

      Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

  • @aashu1565
    @aashu1565 4 ปีที่แล้ว +91

    Thank you sooo much sir for your efforts .... i must say that you are the best biology teacher i ever have

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว +7

      Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

    • @baikunthdwivedy5447
      @baikunthdwivedy5447 2 ปีที่แล้ว

      Abhinav goyal sir ka naam suna ha ?

  • @shereen3566
    @shereen3566 6 ปีที่แล้ว +21

    I have an exam on Thursday on this and I never understood this process until I listened to you explain it. Thank you so so much for your help❤️

  • @misbahmalik9752
    @misbahmalik9752 6 ปีที่แล้ว +5

    This man is MASHALLAH :) I've just started watching his tutorials and getting information that I couldn't get during my B.S and M.S. No one can teach better than him. ALLAH bless u

    • @sarmadghafoor1484
      @sarmadghafoor1484 5 ปีที่แล้ว

      Welllll....allah is best at everything so your wrong XD

  • @devonfernando727
    @devonfernando727 3 ปีที่แล้ว +3

    No professors explained what southern blotting really is. So you are the best professor for me. Thank you 😊
    Good luck sir,
    From Sri Lanka 🇱🇰

  • @MrLongmei
    @MrLongmei 4 ปีที่แล้ว +12

    This is the first ever lecture that I cried out of satisfaction ! As I have quite being long struggling for this topic .Thank you so much sir.😊😊😊

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว +2

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @lauraprince9949
    @lauraprince9949 8 ปีที่แล้ว +77

    Thank you for making this video. You've explained this way better than my prof!

    • @shomusbiologyofficial
      @shomusbiologyofficial  8 ปีที่แล้ว +2

      +Laura Prince thank you very much.

    • @sourabhsingh2934
      @sourabhsingh2934 6 ปีที่แล้ว +3

      My teacher finished in 5 min and i was not able to understand😑

  • @ponktiaddrita6274
    @ponktiaddrita6274 ปีที่แล้ว +2

    You are the best teacher! I am doing undergrad in Biotechnology and class lectures and slides exhaust me. You are the one who is making the graduation process a little less harder! Respect.

  • @shireenroy2642
    @shireenroy2642 5 ปีที่แล้ว +1

    You are a life savior sir! I tried to understand this multiple times for 1and half month couldn't get but now I have got it clearly. U just saved me

  • @lonelygamerzwala4131
    @lonelygamerzwala4131 2 ปีที่แล้ว +3

    Sir you are a savior of my life specially before exam . You teach every portion in a very simplified and lucid way . Always keep us helping like this. Thank you sir.

  • @midnightdreamer1682
    @midnightdreamer1682 6 ปีที่แล้ว

    it took more time to read d same thing from book but just some minutes r required to understand d same thing in more clear way from your lectures...the way u taught d thing and make us learnt is really awesome.

  • @pallavikhele1822
    @pallavikhele1822 6 ปีที่แล้ว +5

    somus biology is one of the most interesting online class an I can able to get any topic easily in my mind I love this channel in the form of understanding level

  • @xurshidbekumarov3461
    @xurshidbekumarov3461 2 ปีที่แล้ว +2

    Thank you very very much. There is no such explanation in the youtube even in 2022. May God bless you.

  • @pleasemusic3314
    @pleasemusic3314 4 ปีที่แล้ว +4

    I am a medical student and you explained it so well. Thank you

  • @rishita_sharma4805
    @rishita_sharma4805 9 หลายเดือนก่อน +2

    Thank you so much sir I'm graduating just by watching your videos you have saved my life ❤

  • @tauseefwani9636
    @tauseefwani9636 3 ปีที่แล้ว +3

    Thanks Sir doing a incredible job towards learning community irrespective of age, degree or position. Thanks a lot. Sir, u r better than most of teachers, Professor's who don't know how to teach learners. Kudos to u Sir.

  • @hippoenitsirk
    @hippoenitsirk 11 หลายเดือนก่อน +1

    I had a chemistry exam today at uni and now i am studying for a molec genetics exam for tmrw and i just wanted to say that god may bless him. 7 yeqrs later and he has helped countless of students.

    • @shomusbiologyofficial
      @shomusbiologyofficial  11 หลายเดือนก่อน

      Thank you so much for appreciating my efforts. Stay tuned for more

  • @minaristic6006
    @minaristic6006 5 ปีที่แล้ว +6

    Great video! I have Southern Blotting as the subject for my biology presentation, and this is the only thing that helped me understand what Southern Blotting is. Thank you!

  • @Gaeforhae
    @Gaeforhae 11 หลายเดือนก่อน +2

    Whenever i need to clear my doubts i type the topic name in youtube followed by shomu’s biology because literally nobody clears my concepts like he does , he is a blessing sent to us by gods 😭😭

    • @shomusbiologyofficial
      @shomusbiologyofficial  11 หลายเดือนก่อน +1

      Glad to hear that you're getting benefit from my lectures. Stay tuned for more. Thank you for your appreciation

  • @mbbslife3075
    @mbbslife3075 4 ปีที่แล้ว +3

    Your teaching style is best sir 🥰🥰

  • @ummekhadiza7288
    @ummekhadiza7288 3 ปีที่แล้ว +1

    I have been watching your vedio since 4 years. Your lessons help me so much to learn clearly about the different branch of biology. Thank you so much sir.

  • @jahnavip1307
    @jahnavip1307 2 ปีที่แล้ว +4

    New subscriber here....I always left out the genetics topics without studing in detail in school, even in puc and degree....but now when I started knowing about genetics in master's degree, your videos helped alot for me to understand the basics and made me to learn and know more about molecular biology and genetics...

  • @sanjidasamadsnigdha7050
    @sanjidasamadsnigdha7050 3 ปีที่แล้ว +2

    You are the best biology teacher. Thank you so much sir. Before all of my exams, your videos always give me a crystal clear concept.

  • @vebexplaeya
    @vebexplaeya 6 ปีที่แล้ว +4

    Thanks bro for simplifying such techniques so efficiently..It is far better than those complex contents given in the books....

  • @heloisemathew3490
    @heloisemathew3490 4 ปีที่แล้ว +2

    Thank u sir for getting concepts clear within minutes..

  • @ACHIEVEWN
    @ACHIEVEWN 8 ปีที่แล้ว +4

    i have been watching your vids since many years now.. and you have really improved in ur explanations... ur older vids are nice as well.. but the newer ones are even better.. i prefer this format over the older s.blot video!

  • @annmartin1242
    @annmartin1242 7 ปีที่แล้ว

    u r that friend who helps us during xams by explaining everything for a person like me who is a visual thinker. thanx a ton buddy

  • @kuchbhicontent2092
    @kuchbhicontent2092 3 ปีที่แล้ว +5

    What an excellent way to teach Man!! And covering all the contents in easy way with Full of Interest!!!
    Shomu Sir hats off to you,
    You are my inspiration, and i wish i will be as intelligent as you, and explain all topics like you
    Ameen
    Tnx for these excellent way to explain topics that makes them interesting.

    • @shomusbiologyofficial
      @shomusbiologyofficial  3 ปีที่แล้ว +1

      Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

  • @sevodar
    @sevodar ปีที่แล้ว +1

    Mr Shomu I had a presentation for university about Southern blot , I didn't get it at all before but ur video literally was satisfying and amazing, thank u sooo much man , I did a rly nice presentation today all based on your video with the same way of explication ❤

  • @meenugarg2443
    @meenugarg2443 7 ปีที่แล้ว +3

    thank you sir... your video has actually cleared all my concepts... this is the first time when i actually understood the exact meaning of probe n need of using nitro cellulose filter..... thanks a lot sir... god bless u.

  • @mikaelanicolecelo4006
    @mikaelanicolecelo4006 4 ปีที่แล้ว +2

    The video made me understand southern blotting very easily. Off to watch northern blotting! Looking forward to more of your content!

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว +1

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @divyeshpant7482
    @divyeshpant7482 2 ปีที่แล้ว +3

    I know I'm late but I have an exam tomorrow (Btech Biotechnology) and this is simply the best video on Southern Blotting on TH-cam. Not only the content but the enthusiasm with which you explain is so good to see that I understood everything and am left with a smile on my face. Hats off to you sir!

    • @shomusbiologyofficial
      @shomusbiologyofficial  2 ปีที่แล้ว

      Thank you so much for appreciating my efforts. Glad you liked it

  • @divineconnectiontarot
    @divineconnectiontarot 7 ปีที่แล้ว

    again after searching so many sites ,I have reached to the best site .....best explanation

    • @shomusbiologyofficial
      @shomusbiologyofficial  7 ปีที่แล้ว

      +chhavi saxena thank you. Glad you liked my lectures. Please subscribe and share

  • @snehasharma9204
    @snehasharma9204 7 ปีที่แล้ว +7

    thank you very much. i have my exams the day after tomorrow and this really helped.

  • @jessicaneamtu9053
    @jessicaneamtu9053 ปีที่แล้ว +1

    This video was so helpful! It was the best explanation on Southern blotting I have ever seen. Thank you so much!

  • @amritarathore2119
    @amritarathore2119 7 ปีที่แล้ว +16

    Amazing teacher.....
    Thank you soo much sir...and respect u a lot.....

  • @jahnvisadrani1394
    @jahnvisadrani1394 4 ปีที่แล้ว +2

    You have made a great explanation sir.Your technique of teaching is very effective that clears many concepts. Thank you so much.

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @sumiyazafar2326
    @sumiyazafar2326 ปีที่แล้ว +3

    Thank you so much sir❤ your way of teaching is very understandable..ALLAH pak bless you🤲

  • @omarmhenni8540
    @omarmhenni8540 5 ปีที่แล้ว +1

    bro u r a thousand time better than professors in my university
    god bless u

  • @tendoeschate
    @tendoeschate 8 ปีที่แล้ว +14

    excellant video... even books also dont explain this well. thanks...

    • @shomusbiologyofficial
      @shomusbiologyofficial  8 ปีที่แล้ว +4

      +indranil bhattacharjee thank you very much. Glad you liked this lecture.

  • @priyapandhare8906
    @priyapandhare8906 2 ปีที่แล้ว +1

    Couldn't go back without commenting,, Amazing teaching!!!!👌

  • @Alex-ml7iz
    @Alex-ml7iz ปีที่แล้ว +1

    I have check severals youtube video looking for some tutoral for my master in microbiology, as a physician i find this concepts very difficult to understand, I am a teacher myself i can notice this is pretty good explaining material you just dont explain the technique itself you relate everything in a way that is easy to learn and you also explain for what is gonna get usefull. Kudos to you. Wish me an approval in my exam

  • @ChrististhereasonLecheKoza
    @ChrististhereasonLecheKoza 3 ปีที่แล้ว +3

    Wow! Best explanation ever. I love your videos. They are so easy to understand and it makes the subject more interesting. Thank you for your efforts sir.

  • @e.mondalsclassroom5209
    @e.mondalsclassroom5209 4 ปีที่แล้ว +2

    Very nice explanation.
    Very much easy to understand.
    Thanks

  • @amishapatel8012
    @amishapatel8012 7 ปีที่แล้ว +3

    Sir your lectures are awesome. Thanks for your such effort.

  • @pavithrakrgowda7975
    @pavithrakrgowda7975 4 ปีที่แล้ว +2

    I think there is no other person who explains like you in a simpler way Even though it is complicated.....

  • @saulatzaba7104
    @saulatzaba7104 4 ปีที่แล้ว +4

    U r awsm sir I am going to see ur all lecture ...to crack CSIR..I need this way of lecture to understand 😊
    THANK YOU SO MUCH

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว +1

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @priyankanandi1173
    @priyankanandi1173 2 ปีที่แล้ว +2

    Your like an gurdian angel sir ...u explain so well .....i watched approximately all of ur videos it's soo easy to understand 💕💕 thank you soo much sir.....✨✨

    • @shomusbiologyofficial
      @shomusbiologyofficial  2 ปีที่แล้ว +1

      Thank you so much for appreciating my efforts. Stay tuned for more

  • @dipayansharma4956
    @dipayansharma4956 7 ปีที่แล้ว +3

    i regret why didn't i find it before... thanks a lot

  • @sanababer4517
    @sanababer4517 5 ปีที่แล้ว +1

    i have no words to say thanks to u ...thank u soooooooo much sir for this effort ..Allah ap ko izat r kamyabia dy

    • @shomusbiologyofficial
      @shomusbiologyofficial  5 ปีที่แล้ว

      Thank you. Glad you liked my lectures. Please subscribe and share

  • @manjulasubash6254
    @manjulasubash6254 6 ปีที่แล้ว +3

    Thank you soo much sir...I love Ur passion about teaching..

  • @aabhaghoradkar6453
    @aabhaghoradkar6453 5 ปีที่แล้ว +4

    Sir when i search my topic on you tube and i see ur video of that topic my tension becomes half becoz u r great and your videos are too much helpful thank you so much sir😊

  • @reshmimishra648
    @reshmimishra648 5 ปีที่แล้ว +3

    ☺️ that's yu sir... ever i get understood all d concept nd i feel Gud fr it... 💯 teaching no error... love wd teaching... untill i saw ur vd i throught wt a sucking topic.. but now im gud wd it... Can score Gud
    ❤❤ 💯... wish u to upload more

    • @shomusbiologyofficial
      @shomusbiologyofficial  5 ปีที่แล้ว +1

      Glad you liked my lectures

    • @reshmimishra648
      @reshmimishra648 5 ปีที่แล้ว +1

      😊😊.. thanku sooo much 💓.. just xam got over wrote Gud

  • @asi6366
    @asi6366 3 ปีที่แล้ว +1

    Sir you are the best. I have followed you for my icar jrf exam. Now I am following you to complete my masters in plant pathology ☺☺☺

  • @dhunganalaxmi8277
    @dhunganalaxmi8277 ปีที่แล้ว +3

    U are a best teacher ever found ❤❤

  • @vaishnavinimbalkar6522
    @vaishnavinimbalkar6522 ปีที่แล้ว +2

    Hello Shomu Bhattacharjee !
    I'm a 2nd year student who had to deliver seminar about the same topic .The whole concept of SBT has been told by you in a very easy and understandable manner , you video has been very beneficial for me , Thanking you for the same .

  • @tanuj...5610
    @tanuj...5610 6 ปีที่แล้ว +3

    Love the way of your teaching..😊😊😊

  • @ganwalb
    @ganwalb 7 ปีที่แล้ว +2

    Today i learnt southren blotting by this video... 😁😍
    Yesterday i learnt Western blotting. Hats off for uploading these type of informative lectures.

  • @nehayadav-on1lv
    @nehayadav-on1lv 5 ปีที่แล้ว +18

    Denaturation of fragmented dsDNA is done before transferring to NItro cellulose membrane. Otherwise how this DNA will attach to the membrane.

    • @batkuparpohtam8070
      @batkuparpohtam8070 5 ปีที่แล้ว

      U r right it should have done before tranfering into nitogenous paper

    • @saneha7020
      @saneha7020 4 ปีที่แล้ว

      Hey can you tell me why those ss dna dont bind to each other again before binding to probe ???

  • @shikidixit5144
    @shikidixit5144 8 ปีที่แล้ว +1

    Southern blotting was a foreign concept to me before I watched this video. Thank you, Shamu....as usual!

  • @beingcreative7817
    @beingcreative7817 2 ปีที่แล้ว +3

    Wow what a lecture..♥️

  • @aneormaetxea3869
    @aneormaetxea3869 4 ปีที่แล้ว +1

    Excelent explanation! Very easy to follow and very clear. Thank you so much!

  • @JoannaPrzybyszewska
    @JoannaPrzybyszewska 7 ปีที่แล้ว +8

    an amazing video!!!!! thank you very much!!!!

  • @she_is_a_hungry_raccoon
    @she_is_a_hungry_raccoon 4 ปีที่แล้ว +2

    you're the number 1 reason i'll manage to get a degree. i'm your biggest fan!
    hope someday i'll learn to be such a great teacher as you are

  • @Jarshivam
    @Jarshivam 8 ปีที่แล้ว +3

    please keep doing these stuffs

  • @indraninayek4892
    @indraninayek4892 3 ปีที่แล้ว +1

    স্যার আপনি অনেক ভালো করে conceptual বোঝান এতে আমার অনেক প্রবলেম গুলো clear হয়ে যায়।। thank u sir

  • @jakkuwolfinsomnia8058
    @jakkuwolfinsomnia8058 8 ปีที่แล้ว +5

    Why do you need alkaline buffer? What would happen if I used acidic buffer, water or Histidine? Why does it HAVE to be alkaline buffer for the imprint to work?

    • @jakkuwolfinsomnia8058
      @jakkuwolfinsomnia8058 7 ปีที่แล้ว

      Thank you ^.^ I understand now

    • @kristinwade344
      @kristinwade344 6 ปีที่แล้ว +3

      dna is negatively charged(phosphate group). alkaline buffer is more basic (ready to share an H+) and acidic buffer is ready to take an H+. in this reaction dna is the acid and is reacting with the basic buffer... this is my thinking..and reference from my orgo chem class... i think the phosphate group which consists of P(-)H3, where the phosphate atom is negative, would give away other H if combined with something more acidic or some compound wanting a H more. Causing the dna to become more reactive.

    • @steadycruisin619
      @steadycruisin619 5 ปีที่แล้ว +1

      Acids contain excess H+ ions, which bind quickly to the phosphate (PO4)- backbone and cancel out the negative charge. Without charge, DNA can't migrate because it won't be attracted to the (+) electrode. The alkaline buffer does the opposite, neutralizing any H+ (or Ca2+, Mg2+, or other + ion) by pulling them off the DNA, into solution. Alkaline means excess OH-, so by dissolving any (+) ions, it makes sure that the DNA will be (-) and be attracted to the positive electrode (allowing migration and development of bands).

  • @ruchitapatel5316
    @ruchitapatel5316 3 ปีที่แล้ว +2

    Your videos are really amazing and life saving for exam preparation.

  • @waseemgilani6085
    @waseemgilani6085 8 ปีที่แล้ว +5

    Great man

  • @FatimaZahra-nx4zx
    @FatimaZahra-nx4zx 6 ปีที่แล้ว

    Ur super duper a1 .... ur way of teaching is very easy... u usually don't left any questions in our minds we got whole fully info about our desire topics...

  • @peepdi
    @peepdi 5 ปีที่แล้ว +3

    Sir I am have a doubt.
    How do we know that the DNA of the required sequence is 100% present?
    Cause we have prepared complementary probe of that sequence right? How do we know that's sequence is present? :/
    Also we can do sequencing to know whether it's there or not? I just don't understand.
    And also sir can you please explain regarding restriction mapping and restriction digestion. Cause I've watch all of those videos but you haven't mentioned about the partial digest double digest and all. And no one can explain better than you its a requesttttttttttt. And i am in th ir d year biotechnology can you suggest reference books for RECOMBINANT DNA TECHNOLOGY.

    • @malikmudasir3568
      @malikmudasir3568 5 ปีที่แล้ว

      actually as far as i know we do sB in order to confirm the gene of interest.
      we have gene banks where we have sequenced the genome
      and we make the probes against the segments whose secquuences we have already done
      and because of hybridization technique ie probes we can diagnose

    • @malikmudasir3568
      @malikmudasir3568 5 ปีที่แล้ว

      I hope u have got it
      this fanda is diagnosis ☺️

  • @sehar573
    @sehar573 10 หลายเดือนก่อน +1

    Your lecture are easy to understand and you are amazing 👏

  • @meghasharma4936
    @meghasharma4936 7 ปีที่แล้ว +3

    But what if the restriction endonuclease (DNase I ) cuts in between the target DNA , Then how our probe will bind to target DNA?

    • @tanyaguduru2606
      @tanyaguduru2606 7 ปีที่แล้ว +4

      megha sharma the restriction endonuclease cuts in specific places only. If you know the sequence of the target dna then you can control which endonuclease to use to cut in what location.

    • @julianunez7489
      @julianunez7489 7 ปีที่แล้ว

      creo que la enzima de restricción solo corta en ciertas partes.

  • @shahbaloch4814
    @shahbaloch4814 3 ปีที่แล้ว +1

    Sir you teach very will and the best method of teaching very helpful best teacher from the youtube great sir keep making videos we will be supporting you Brother

    • @shomusbiologyofficial
      @shomusbiologyofficial  3 ปีที่แล้ว

      Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

  • @aishwaryad7618
    @aishwaryad7618 7 ปีที่แล้ว +3

    Don't we use nylon membrane in Southern Blotting and nitrocellulose in Western Blotting? Im confused..

    • @shadowxxrami6669
      @shadowxxrami6669 7 ปีที่แล้ว +1

      Aishwarya D my same initial thought . can anybody explain ? I'm confused

    • @jeevankishore8196
      @jeevankishore8196 7 ปีที่แล้ว +5

      Aishwarya D either nitrocellulose or nylon membrane can be used in both the technique .

    • @ner0xic537
      @ner0xic537 6 ปีที่แล้ว +1

      both can be used

  • @anooptitus6945
    @anooptitus6945 6 ปีที่แล้ว +1

    Awesome video :) Keep up the great work! Im a doctor and I love teaching too, and your videos show me how to be a better teacher. Cheers!

  • @ОбычныйПерсонаж
    @ОбычныйПерсонаж 6 ปีที่แล้ว

    Finished the video, thanks so much again. Can't stop complimenting you. Such a job has been made... I hope you're continue progressing, evolving.

  • @sudipnale7664
    @sudipnale7664 4 ปีที่แล้ว +2

    Thank you sir soo much due to this lock down I was not able to understand blotting in online lectures from my college but now you have completely made me understand.

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @tharindulakshanbandara7625
    @tharindulakshanbandara7625 2 ปีที่แล้ว +2

    Thank you so much sir.. your very talented and u can teach very clearly and simply.. Better than some prof.s Well done good job.. Again THANK YOU SOO MUCH..!!!

    • @shomusbiologyofficial
      @shomusbiologyofficial  2 ปีที่แล้ว

      Thank you so much for appreciating my efforts. Stay tuned for more

  • @prajakta7817
    @prajakta7817 7 ปีที่แล้ว +1

    Thank you so much...You explained very well...It really helps me to understand the whole process thoroughly...😊 But the only thing that I think you miss to tell is the washings of the membrane filter which is important for removal of unwanted stuff....

  • @sanjidaislam5848
    @sanjidaislam5848 4 ปีที่แล้ว +1

    I am a student of biotechnology. Thise class is so helpful. Thank you sir

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว +1

      You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

  • @estheradigwe3440
    @estheradigwe3440 4 ปีที่แล้ว +1

    You made this much easier to understand, God bless you sir.

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @megashree60
    @megashree60 3 ปีที่แล้ว +2

    Thank you so much for this lecture sir.. Love from Tamil Nadu 😇😇🙏🙏

    • @shomusbiologyofficial
      @shomusbiologyofficial  3 ปีที่แล้ว

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @priyachowdarybejawada9519
    @priyachowdarybejawada9519 4 ปีที่แล้ว +1

    All your lectures are constructive thank you sir...

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @sumayahihsan3016
    @sumayahihsan3016 4 ปีที่แล้ว +1

    Ur videos are helping me out in my finals... God bless u.... 😇

  • @sen6277
    @sen6277 3 ปีที่แล้ว

    Easy to understand after your explanation sir. Thank you🙏

  • @revathyb5526
    @revathyb5526 6 ปีที่แล้ว

    you are entire indian biotech student's best teacher....

  • @AbhishekSharma-cm2st
    @AbhishekSharma-cm2st 3 ปีที่แล้ว +1

    Your lectures are so much concept clearing sir
    Two doubts i have from this lecture
    1. How to place the gel on the buffer as it is fragile??
    2. How to confirm that all the DNA fragments from gel had migrated to the nitrocellulose paper??
    Thank you for clearing concepts of me and many lakhs of students

  • @manpreetsandhu2483
    @manpreetsandhu2483 4 ปีที่แล้ว +2

    Thank u sooo much sir for providing good information about any topic your all classes are very intesting😊😊😊😊

  • @duaabbas3628
    @duaabbas3628 6 ปีที่แล้ว +1

    You have so much knowledge and the best thing is you know how to share it as well, keep up the good great.. you're really amazing 💎💎

  • @rushipharmacology9934
    @rushipharmacology9934 4 ปีที่แล้ว +1

    Superb conceptual way of teaching...

  • @SarojTamang-kv2ne
    @SarojTamang-kv2ne 2 ปีที่แล้ว +1

    One of the best teacher ever💗

  • @sorenaspartacusthegreat1982
    @sorenaspartacusthegreat1982 4 ปีที่แล้ว +2

    Thank you so much, you are one of the best Biologist

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว

      Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

  • @funnytakarar4105
    @funnytakarar4105 6 ปีที่แล้ว +1

    Your lecture help me to understand boltting ...thank you Soo much