Site directed mutagenesis

mutagenesis lecture - This lecture explains about the site directed mutagenesis including other techniques of mutagenesis like site specific recombination and transposition.
Site-directed mutagenesis is a molecular biology procedure that's used to make particular and intentional changes to the DNA sequence of a gene and any gene products.
Site-directed mutagenesis is likely one of the major techniques in laboratory for introducing mutation into a DNA sequence.
This video lecture deals with the mechanism and steps of site directed mutagenesis and the importance of side directed mutagenesis.
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[@netaburnum1359]

Great lecture on the topic of site directed mutagenesis, thanks for the share, @Shomu's Biology.
Alternatively, it is used for investigating the structure and biological activity of DNA, RNA, and protein molecules, and for protein engineering. ---by Profacgen.

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[@harshabharwani4394]

Very well explained. Doubts clarified. Thank you!

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I am also biotech student

[@lanoanhduongthi3536]

Explain very easy to easy understand. Thank you sir

[@TheUsermam]

Thank you for nice explain. I'm just start study about this topic and I'm so confuse. If you and your team have enough time can you explain about Iterative saturation mutagenesis?
Thank you

[@aldocastellani3536]

very helpful video. Very clear as usual. Thanks Shomu!

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[@hebaalghol4497]

Thank you very much.
I would like to ask if you do have a video about Triplet repeat primed PCR (TP-PCR), or if you can recommend a book or website that might help me understand it in details and thank you in advance.

[@shivpuranupay5840]

Beautiful explanation as always

[@marammokhtar1799]

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[@spulwasser]

Nice work ! Now that I understand what was meant with "nicks", I can also imagine how the thing works with deletions and insertions. Thx a lot

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[@chriscarbe1042]

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[@shomusbiologyofficial]

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[@aratimaurya8464]

Nice explanation...I am mathematics student in Bsc. Now doing Ph.D in biochemistry so I have lots of problems in basic of biology...please suggest something how I clear my doubts?????😔😔😔

[@anythingsahaj7467]

i see a drastic transition your course subjects ? any particular reason for biology preference ?

[@swastikmukherjee9648]

can I introduce 3-5 point mutations in this way? Also, do i have then design overlapping primers?

[@nickrhoades2228]

Would the nicks cause a transformation efficiency decrease? Would it be better to use T4 ligase prior to transformation?

[@sharvanisivakumar4792]

Thank you SO much - this was so clearly explained!!

[@shomusbiologyofficial]

You're welcome

[@bharathraj5862]

Sir, what is the difference between the Site Directed Mutagenesis and Single Nucleotide Polymorphism?
Please clarify

[@rafiullah8351]

AOA dear Shomu! What will be the result if only a single primer is used with a change in a single nucleotide please. Thanks

[@raquel838]

muchas gracias aunque no hablo imgles me ayudo mucho....

[@rishabhchandna787]

Sir can u explain how the primer will bind to the double stranded DNA ?????

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[@ivannabby15]

This is so helpful!! thank you for making this video

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[@malabikabhowmik7933]

Very well explained, thank you so much Sir

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[@kashafseducationalsite363]

one confusion sir.. how will this primer bind with dsDNA..? do we have to first denature the dsDNA of vector.. as usual occur in DNA replication...?

[@hamzamasood9933]

bht acha explain keya h AP NY , but kindly types of mutagenesis bhi add kijye , thank u

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[@marilabrune1850]

thank you very much. m from algeria and this video helped me a lot

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[@andresparra747]

Hmm, I think for this overlapping PCR you forgot to talk about kunkel or Quickchange approach in more detail, downstream treatments such as DpnI or enzyme digestion are quintessential for the application and sometimes is better to add the disadvantages such as mispriming or primer dimer hybridization. A part from that you are always in every search on youtube.

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Thank you. Glad you liked my lectures

[@yayahwinarti95]

I still wonder, if we would like to see the effect of mutation of a human gene, so does it mean we should insert the gene first on the vector and then we design a mutated primer for this gene?

[@vibez9630]

is the klenow fragment that seals the nick instead of ligase?

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[@julikavolkmann288]

could you please make a video about signature-tagged mutagenesis? :)

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watched this for my upcoming biotech exam on july 20th !

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It was fine

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Good job bro!

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Excellent teaching thank you

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[@sidrakhan8589]

How to separate the mutated double stranded Dna from the parental dna that of plasmid???

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[@Abir_32133]

Sir how two newly synthesized strand are combined🤔..

[@manognakuchibhotha7018]

sir i have a doubt that in the video at around 4:28 you have said that its different primer sequencing and is not same as PCR but again at last of the video you had presented it as a PCR method..... is it a PCR based primer designing method or no?

[@rasikakute537]

Very nice .. thank you!

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[@debbiesoto4664]

tysm ly Shomu

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[@sofiane4148]

Thx sir
Can you help me with mutagenese with pcr! !

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[@sahabuddinsahabuddin6216]

Thank you very much !

[@shomusbiologyofficial]

You're welcome

[@sunmathyk2360]

please do videos on PCR based site directed mutagenesis & cassette mutagenesis

[@archanasatheesan7064]

Sr plzz upload a class on clb method of mutation detection

[@carlostettey2889]

How do i set the thermocycler to achieve this mutation?

[@dipikasonkar8565]

sir if we will change the particular sequence for mutagenesis their is a possibility of occurence of some genetic disease also ?

[@kashikachowdhry2078]

Thankyou so much sir

[@shomusbiologyofficial]

You're welcome

[@nrzart1612]

Sir, is it same with Tilling method?

[@twisampatidas9965]

Excellent ❤🎉

[@shomusbiologyofficial]

Thank you

[@surjitdeka7092]

Other parts are available or not sir

[@asimkumarroy7471]

Sir, if you elaborate the full and another steps of Site Direcred mutagenesis. It will must help us to a broad idea about these topic. Thank you.

[@shomusbiologyofficial]

Okay

[@sonan2187]

I didn't got your point....when usually replication occurs the daughter cell will have one parental and one new strand ....so how in this both newly formed joined??

[@pushpasharma1813]

mahrukh mahboob the newly formed sequences are mutated and are exactly complementary to each other the reason for which they form duplex

[@rabindrakhatiwada3558]

Sir I need linker DNA mutagenesis I am not clear about it please explain.

[@lakshmiamruthavalli7791]

Tnqu sir 👨‍🏫👨‍🏫

[@akashvani3836]

Sir, please make a video on conventional mutagenesis

[@suyashsachan74]

Loved it

[@shomusbiologyofficial]

Thank you

[@zohrahashmi9488]

How will u take the mutated plasmid

[@writerexpertqueen1724]

Thank you sir👍

[@shomusbiologyofficial]

You're welcome

[@user-go6xz4rb3f]

Thanksss for video

[@shomusbiologyofficial]

You're welcome

[@Abir_32133]

However , sir pls make a detailed video on pcr based site directed mutagenesis including.. overlap extension method,megaprimer PCR ,incerse PCR

[@shomusbiologyofficial]

Okay

[@shraddha8098]

Thank you ❤️

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[@kashafseducationalsite363]

well explained .. from Pakistan..

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[@swatimishra7565]

Thanks sir.... It's really very helpful for me

[@shomusbiologyofficial]

You're welcome

[@naushinahmad8880]

explain random site directed mutagenesis plz