Excellent job sir👍👍👍 You have good technical knowledge .You explained PCR in such simple way that I am finding easy to learn all this.Your hindi too is good.
Your Hindi/,Urdu video are much more good for understanding. Even though English are also v well elobarated. What about starting a separate new channel of shomu biology Hindi
Excellent efforts...special thanks❤❤❤ i have one question, where does the primer attach the DNA strand in reverse PCR??? Because when known sequence is in mid, primer is attached at its sides and continues to add nucleotides further next to itself in the direction of known sequence but now the known sequence is at ends so how will primer be attached???
Awsm sir ...thanku so much jo ap hm logo k liye itna struggle krte hai ...thanku sir ..God bless u Sir mujhe apse ek Favour chahiye plzz help us ... Sir microscopy ke kuch video ap jaldi krne ka try kr skte hai ... I m sure apki list me to hoge hi ye topic but me thoda jaldi krne ka abhi favour mag rhi hu ...i hope u accept my request..thanku sir
In the case of inverse PCR, if the sequence at the end are unknown then how do we identify the desired restriction enzyme to cleave and form sticky ends prior to the formation of circular DNA?
Moreover in inverse PCR the DNA molecule used initially is basically wat a whole genomic DNA or a small part of it. Coz of its whole genome then adding a restriction enzyme may produce several small segments. How will we then isolate the desired one to be used for inverse PCR.
Sir in nested PCR , then what is the need of normal primers? we can directly add the nested primers on the target sequence and amplify our desired gene. Then what is the need of normal primers then?
Sir i have a question related to nested pcr.. That we are adding both outer and nested primers in a definite concentration... It is true that outer primer will amplify the unwanted DNA and nested one will amplify the target DNA.. But the whole process is going on for 30 cycles lets say... And the unwanted DNA along with outer primer they too are in the reaction till the reaction ends for 30 cycles how come we are going to identify wheather the unwanted DNA is not amplified as the reaction has outer primer and DNA till the end.... I mean are we adding any enzymes that will. Specifically cut the target DNA from the rest unwanted DNA
Sir as we designed the second set of primers for the target dna in nested pcr why can't we designes the specific primers of the target dna for the common pcr.....sir kindly answer i really wanted to know
There is no such thing as only for research purpose if it is used in research, and found to be effective, we must use them for diagnostics every single diagnostic test that we see these days were sometimes a part of research work
Nested always keeps me wondering ABT it's need. Wn one already has the inner primer set then y to use the external one. Anyway practically things are not so common wd the theoretical aspects.
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Beautifully explained
Thank you
You are the best Sir! I salute your efforts towards teaching us with such integrity❤
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Awesome.....thnks....aapke videos bhut achhe hote h nd helpful hote h👏👏
Glad to hear that you are getting benefit from the videos
I like your Hindi you explained very well in Hindi 😍👍🏻
Thank you. Glad you liked my lectures
You’re the best sir!!!!!!!! You should get prize in biology 🧬
Thank you so much for appreciating my efforts
yes..he should be awarded
Yes indeed itny difficult topics itny asan tareqy sy explain kiye ha
He has csir net qualified
Sir I love you soo much ...mne bahut wait Liya ki kB aap hindi m daaloge...am soooooo happy ..thanks
Glad I can make you happy to learn
Really superb... Mr Suman. God bless you 😇
Thank you so much for appreciating my efforts
THANK YOU SO MUCH SIR ! U R THE BEST BIOLOGY TEACHER ON TH-cam
Thank you so much for appreciating my efforts
Sir you are amazing ...thank you sir
Thank you sir very nice explain in hindi ,,
🙏🙏
It was explained superbly❤❤❤
Excellent job sir👍👍👍 You have good technical knowledge .You explained PCR in such simple way that I am finding easy to learn all this.Your hindi too is good.
Thank You're very much for appreciating my efforts
Thank you for explaining the things in Hindi
You're welcome
Very nice explanation sir
You're welcome
Very well explanation sir.. Clearly understood by anyone.. Your way of explanation is so clear nd smooth... Thnku so much sir...
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@@shomusbiologyofficial sure sir..
sir your hindhi is best ever
Thank you
Shomu's Biology
si pls make a video on how to write thesis in scientific writing
pharaphases in hindhi pls pls
pls sir
Best of the best
Thank you so much for appreciating my efforts
First to watch. Thanks for every videos sir. I am going through your videos all my semesters. ♥️
Glad you liked my lectures
Greatest ever explanation Sir.
Thank you so much for appreciating my efforts
Welcome sir
Nice explanation
Thank you
Salute to you 👍👍👍🙏🙏🙏🙏🙏
Thank you so much for appreciating my efforts
Your Hindi/,Urdu video are much more good for understanding. Even though English are also v well elobarated. What about starting a separate new channel of shomu biology Hindi
Thank you
Thankyou so much sir 🙂
You're welcome
Very well explained 🙏🙏
Thank you so much for appreciating my efforts
too good sir
Thank you
Sir itna easy bana diya❤️
You're welcome
thanks sir hindi vedio bnaane ke liye
Hats off 🙏🙏🙏🙏 sir thanks for this video
Excellent efforts...special thanks❤❤❤ i have one question, where does the primer attach the DNA strand in reverse PCR??? Because when known sequence is in mid, primer is attached at its sides and continues to add nucleotides further next to itself in the direction of known sequence but now the known sequence is at ends so how will primer be attached???
very nice vdo loved it
Thank you so much for appreciating my efforts
Sir your teaching is just incomparable to anyone ....please can U explain ITS region Nd related topics
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
Very very nice 👏👏
You're welcome
Gjb sir
such me bahut hi achi vidio hai wo bhi hindi me
You're welcome
make ur profile on instagram too sir ...so that more people get to
know about you ...and get benefited by watching ur vdos..
Sure
Thanku sir..
It's really useful.
You're welcome
Awsm sir ...thanku so much jo ap hm logo k liye itna struggle krte hai ...thanku sir ..God bless u
Sir mujhe apse ek Favour chahiye plzz help us ...
Sir microscopy ke kuch video ap jaldi krne ka try kr skte hai ...
I m sure apki list me to hoge hi ye topic but me thoda jaldi krne ka abhi favour mag rhi hu ...i hope u accept my request..thanku sir
I will make many more videos in hindi
Thankyou so much sir
Thank you sir achha hai
You're welcome
U speck very nice hindi,
Thank you
Thanku very much sir for all videos
Nice video sir.
You're welcome
Aamr akhon Mone hoi.. amr jibone zoology r kono chapter e r kono somossa hbena .. karon shomus biology ache ✌️✌️✌️✌️
I am glad to hear that you're getting benefit from my lectures. Stay tuned
In the case of inverse PCR, if the sequence at the end are unknown then how do we identify the desired restriction enzyme to cleave and form sticky ends prior to the formation of circular DNA?
Same question
Thanks sir
You're welcome
Wow Sir your hindi 👌👌👌
You're welcome
Nice hindi sir
Thank you
Awesome
You're welcome
thankyou sir
You're welcome
Thanku sir make a video in Hindi medium ..... I wish u will make a video Hindi in future
Sure
Sambhu bro I love you
Thanks
Thank U sir........
Nice sir ji 🤗🤗 PDF mil sakta hai Kya
Sir please make a video on Carrier detection in Haemophilia A PATIENTS by using linkage markers
I am so used to your english videos that its weird to watch you explaining in hindi!😂
Yas
Moreover in inverse PCR the DNA molecule used initially is basically wat a whole genomic DNA or a small part of it. Coz of its whole genome then adding a restriction enzyme may produce several small segments. How will we then isolate the desired one to be used for inverse PCR.
Sir... Please make a video about how to help pcr for diagnosis disease
Okay sure
Sir, kindly make a tutorial on lymphocyte trafficking.. I really need to understand it..
Nice sir
Thank you.
Hello sir .
semi-nested pcr pr b vedio bna de .iska concept clear nhi ho rha
Plz presentation on blast and fasta
In Rt pcr why rna primer is used instead of dna primer?
While in other type of pcr dna primer is used
Please reply
I want to understand Degenerate PCR primer designing..Could you??
Sir in nested PCR , then what is the need of normal primers? we can directly add the nested primers on the target sequence and amplify our desired gene. Then what is the need of normal primers then?
If in the question paper given RT PCR then it is real time or reverse transcriptase PCR ???
Sir i have a question related to nested pcr.. That we are adding both outer and nested primers in a definite concentration... It is true that outer primer will amplify the unwanted DNA and nested one will amplify the target DNA.. But the whole process is going on for 30 cycles lets say... And the unwanted DNA along with outer primer they too are in the reaction till the reaction ends for 30 cycles how come we are going to identify wheather the unwanted DNA is not amplified as the reaction has outer primer and DNA till the end.... I mean are we adding any enzymes that will. Specifically cut the target DNA from the rest unwanted DNA
by gel electrophoresis. best example of nested PCR is identification of Rotavirus
Please suggest any book of molecular biology i m bsc mlt student
Nice video sir ekad uhi hindi m b video bana dijiyega
Thank you. Glad you liked my lectures
Sir as we designed the second set of primers for the target dna in nested pcr why can't we designes the specific primers of the target dna for the common pcr.....sir kindly answer i really wanted to know
Flanking sequence means sir
Means the sequence present as single stranded region of DNA
Shomu could you make this video in English as well please
It's already there
thk u sir
You're welcome
Sir, can you plz upload csir life science videos
Many videos are uploaded. There are more than 2300 videos
Texonomy k upper video upload karo please
I will.
if no plz tell the difference between nested or hemi-nested pcr
Why we don't use just nested primer ? Why we use 1st primer if it binds unwanted sequnces?
One primer set can make more unwanted amplification. Two set can reduce unwanted amplification
Flanking sequence we know
Ur hindi is not that bad sir...... 🙂 ur method of teaching best in all the ways
Thank you
Sir plz give complete information about ICAR - ARS exam
Sir ,types of PCR ,csir net me pucha jata h kya?
Yes
Late PCR kya hai please video sir
Okay
Kya PCR hiv test me bhi kaam aata hai, or iski accuracy kitni he
is hemi-nested or semi-nested pcr same?
👌👌👌☺
Thank you. Glad you liked my lectures
what type of polymerase we use in real time PCR as in taq man probe it uses exonuclease activity
Probe or dye
RNA RT-PCR is confermetory test and target not detected means
Can we use same pcr for testing dna of different species i.e human ,plant ,bacteria
Different chambers
Evolution video in hindi please sir
👍👍👍
I think
Ye topic eng m cover ho gya h?
Ues
Sir notes mil saktey hai?
Kitna DNA amplify kr rha hai, we can also measure it.
In quantitative real time pcr
Can you make videos in english plz
You will get this video in English in my channel as well
i need notes of this video urgently. can anybody have?
Sir agar iska test kit research purpose ke liye he but diagnosis purpose ke liye nahi he to aaj ki date me isko diagnosis ke liye kyo use kar rahe he
There is no such thing as only for research purpose if it is used in research, and found to be effective, we must use them for diagnostics every single diagnostic test that we see these days were sometimes a part of research work
Sir aap BSC zoology honours ki whole video hindi me bnao either payed video ya free depend on your .....bt mghe need h kaise hoga pls help me sir..
Nested always keeps me wondering ABT it's need. Wn one already has the inner primer set then y to use the external one. Anyway practically things are not so common wd the theoretical aspects.
Same here....
I am still not able to understand nested PCR 😢
PCR English plz
Already there
Will I eligible for UGC net exam after the M. Tech in cosmetics..?
sir ap pls hindi me bhi kuch vidio banyakariye pls ☺
I am making more videos in hindi