Thank you very much! We have ImageJ on our School PCs and we are very glad that we found your video. If we had not found it, it would have taken us hours to understand this program. Thank you
Thanks for the tutorial! Do you know if it is possible to make the lines permanent? I need to measure the length of many microtubes in my picture and it's a bit hard to do it because since the line disappears every time a draw a new one, I may forget if I already measured a certain tube =/
However the closest solution would be ROI manager. After drawing the first line, press "t". A new window will pop up name ROI manager. It list the first coordinate. Check the Show All box for all the measured lengths in ROI Manager. Repeat the process until all tubes been scaled. Only then press onto the Measure button or "cmd+M". Hope it helps. Please help Like my video by pressing the thumb up button. Thanks !
Very useful. BTW, your average (in Summarize) also used the scaling measurement which does not make physical sense. Perhaps it would be nice to show how to delete the unwanted measurement!
Hi, im working on the research about the impact of droplet on a spherical surface using high speed video camera. So basically, the video camera recorded the droplet dropping from a specific height (say 14cm). Im having trouble trying to calibrate the velocity based on the distance from the video. Im totally new to Fiji and it would be much appreciated if you could give me a tinkle :)
Calibrate the velocity ? erm…. IF the system has a timer by knowing when water droplet is released, Then, isn't it the timer system supposed to synchronize with the high speed camera ? Or at least, the location of the camera is half-way tangential to the path of the droplet before the curve surface. If that is true, then the film rate is what you need ! By knowing the film-rate in million-frame-per second and relating it to your video could easily calculate the time for impact. Then speed could be obtained by dividing time by specific height (14cm instance). Hope i shed some light.
nice video . but i am having a problem regarding the scale of the picture.i just have a picture of a river and i want to measure it's width. Can you help?
Sauhard Joshi Erm... Any common length seen in within picture? A dinghy park near the river bank, a stick, a big stone (could be measure at site) etc...
+shabiha siddiqua Hello there, Do you refer to the two lines of white color wording ? It is an setting option choose from the electron microscope's control panel. Include a scale bar.
Hi there ! I have no idea the size range. One way worth trying would be to benchmark it with a known size object within the image. For example, you may want to benchmark the overall length of your sample. If the sample looked under the microscope is distributed over a circle, then the diameter could be used as the scale for you to estimate the size. The fundamental of how the algorithm works is based on the know length compared to the amount of pixel across the measured length. Another way would be to calculate the size using the magnification. Worst come to worse need to use this method. Try Google for "calculate the size with magnification scale" Hope it helps !
Thanks for making a easy to understand video! I was wondering if you could help me measure the distance between two points on a curved path using ImageJ? I have a curved line in one binarized channel 1 and dots along the line in another binarized channel 2. The ROI feature is useful for taking the matrix positions of these features and I found that flattening the image combines my features, but I want to measure how long the path is between points in channel 2 following the path of channel 1. Is there a feature in ImageJ that would do this?
April Peterson It is possible to tell if the scale for both channels are the same? Is an apple to an apple comparison. I am not sure of any masking features available. Maybe need to mask the path at Channel 1 onto Channel 2. Maybe i could help more if i have an example of the above mentioned problem.
Liew Seng Choy the scales across the channels are the same. The images I am using are immuno-histochemical florescence of a tissue spread. I actually have 3 channels which are florescence signals of different proteins. I'll try to describe the example, because I can't upload the data. Imagine a top down view of a snake (curved line in channel 1), the head is represented by a circle (channel 2) and there are 3 spots along its back (channel 3). I want to measure the relative distance of the spots to the head and to each other. I hope that makes sense, thanks in advance for any insights or tips!
April Peterson I wish to help. THe closest possible solution would be Image > Overlay> To ROI Manager. Condition is that your file has to be in tiff format. I have previously made a video about multiple length. th-cam.com/video/i3pnG-37b8I/w-d-xo.html IN addition to that, save the file into tiff. WIth all four yellow circles (since it is now circle) which labeled in ROI Manager. Then, draw straight line between spots for individual distance. Press onto the Measure button in ROI Manager for all length. Let me know if i managed to solve your problem.
Thank you for this video, it's very helpful. But I want to ask, how were you able to get the known distance to be 01, and how can I apply it to my case. Thanks
Could you please let me know if I can automatically calculate multiple lengths in a single image? I have a distribution of nanorods (approx. 75 in each image) and need to find out the average length. Thanks.
Liew Seng Choy I am stuck at getting that data itself. Right now I am calculating the lengths manually. Please let me know if there is any option in imagej to automatically find out the length of all such rods in an image. If you can provide me your email id I can send you one of the images to clearly point out the problem. Thanks
Sankalp Verma I might be able to shed some light on the issue. Word of advise would be to discuss with your supervisors about this. The following method is one of among many possibilities to address the problem. Before i made my personal suggestion, manually getting 75 rods per micrograph isn't really bad if we are talking about maybe 15-20 micrograph images, A big monitors is all you need. In addition to that, results obtained could be plotted into histogram (th-cam.com/video/2NrT7aI9SJk/w-d-xo.html) i made one video about setting up histogram. You may of course refer other video in that topic, It is more convincing once presented into your dissertation as a histogram. It means, very single strand is manually verified. Unless you have really huge amount of data to process. Otherwise, Using image processing toolbox in MATLAB could be a solution. (Word of advise would be to consult your supervisor about it) First, it is not a freeware. That software required license to work. Second, it takes some few weeks to get around how the programming languages works. Not hard but quite tedious. Then, only it comes to using the toolbox. It may takes a few weeks before getting result that might not be promising. Would be way much feasible to do it manually. Try use the short cut key (Opt-M) to measure each length, 75rods could be easily done within 45minutes using 21inch monitor ! The problems with writing a program for me is that I'm not very well trained in A.I programming, Problem arise when object overlap ! Try discuss with your supervisor. Maybe there are other solutions.
Liew Seng Choy Thanks for the suggestion. My data set is quite huge so doing it manually would take a lot of time. I have access to MATLAB so would give it a try. Thanks again.
Thank you. Trying my very best to get a quality microphone in my future video. The one I had was the one I could afford back then while I was doing my postgraduate study. Hope by very soon I can have good quality voice video to share and to help many more.
Thank you very much for this tutorial! Even 9 years later, still remains useful and accurate.
Thank you.
太感谢你啦~我是个医学生，在做毕业课题，需要根据图片和标尺测量图像长度，你的教程完美解答了我的疑惑！
谢谢你的留言。 分享出去帮助更加多同学吧！
Thank you very much! We have ImageJ on our School PCs and we are very glad that we found your video. If we had not found it, it would have taken us hours to understand this program.
Thank you
You are welcome. It was intended to help remind myself how to use this software after some time later. Good to know it helps your school.
Thanks so much for this video- I've been using it for years with my students so they know how to do this for our research.
I do refer to this from time to time when I need to measure a length from an micrograph. Not using this software on regular basis.
thank you so much. I am self learning nanotechnology and it is videos like these that help me so so so much.
from Boston, USA
Thank you for watching this video.
Did you include scale while taking photograph?
The lower middle white line represent 100 micron length.
Thank you! You explained it very clearly and I loved your accent! :)
Help subscribe to my channel for making it bigger.
Thanks for the tutorial!
Do you know if it is possible to make the lines permanent? I need to measure the length of many microtubes in my picture and it's a bit hard to do it because since the line disappears every time a draw a new one, I may forget if I already measured a certain tube =/
However the closest solution would be ROI manager.
After drawing the first line, press "t". A new window will pop up name ROI manager.
It list the first coordinate.
Check the Show All box for all the measured lengths in ROI Manager.
Repeat the process until all tubes been scaled.
Only then press onto the Measure button or "cmd+M".
Hope it helps.
Please help Like my video by pressing the thumb up button.
Thanks !
th-cam.com/video/i3pnG-37b8I/w-d-xo.html
Made a video for the above steps.
Very well explained and showed, so helpful! :) Thanks!
Subscribe to my channel to help make it growth.
Thanks, this video is going to be helpful during my thesis writing.
Good too know it is useful.
There is another video on getting those data into histogram.
Hope you will like it.
Thank you for the video, it was very self explanatory. Can you please tell how can I save the Marked up Image and the ROI?
Thank you for putting in all good words.
My another video might be able to help you:
th-cam.com/video/i3pnG-37b8I/w-d-xo.html
Very useful. BTW, your average (in Summarize) also used the scaling measurement which does not make physical sense. Perhaps it would be nice to show how to delete the unwanted measurement!
+Jan Hough
Hello there !
Thanks for the advise.
Perhaps my another video could be useful.
th-cam.com/video/i3pnG-37b8I/w-d-xo.html
Thank you so much for making this video! It really saves my life :)
=)
Thanks, this was very helpful! Greetings from Germany!
SurrealThought
Thank you.
Was actually a record made to remind myself initially but turns out useful for others involved.
Very well explained! Can you please suggest hw to measure if one has different pictures with no scale bar
Really unable but to capture another new picture with a reference scale.
Thank you!! Very helpful! Now... on to measuring!!
How are you? Any project you are working on?
you are a life saver, thanks
Thanks for your support. I’ll continues sharing more videos that help more people.
Thank you for a nice tutorial. I can easily follow the steps and its help alot since its a little bit confusing refering the manual. Haha
You are welcome. Subscribe to my channel for easier future reference.
Hi, im working on the research about the impact of droplet on a spherical surface using high speed video camera. So basically, the video camera recorded the droplet dropping from a specific height (say 14cm). Im having trouble trying to calibrate the velocity based on the distance from the video. Im totally new to Fiji and it would be much appreciated if you could give me a tinkle :)
Calibrate the velocity ?
erm….
IF the system has a timer by knowing when water droplet is released,
Then, isn't it the timer system supposed to synchronize with the high speed camera ?
Or at least, the location of the camera is half-way tangential to the path of the droplet before the curve surface.
If that is true, then the film rate is what you need !
By knowing the film-rate in million-frame-per second and relating it to your video could easily calculate the time for impact. Then speed could be obtained by dividing time by specific height (14cm instance).
Hope i shed some light.
Liew Seng Choy
thanks heaps for your help!! :D
nice presentation. please try to do more and more presentation . thank you very much .
+Swagotom Sarkar
You are welcome.
There are a few more relevant videos in the playlist. Check if it is useful as well.
Thank you for your nice presentation
Thank for subscribing to my channel. Super thanks feature is now available. Thanks for viewing.
Thank you, sir, really I look for this and get benefited after watching this video
+Lakkoji Satish
Appreciate the thumb up !
God to know it help another person.
nice video . but i am having a problem regarding the scale of the picture.i just have a picture of a river and i want to measure it's width. Can you help?
Sauhard Joshi
Erm... Any common length seen in within picture?
A dinghy park near the river bank, a stick, a big stone (could be measure at site) etc...
hii...very useful video but can u please explain how did u get the stub measurement on the image
+shabiha siddiqua
Hello there,
Do you refer to the two lines of white color wording ?
It is an setting option choose from the electron microscope's control panel. Include a scale bar.
+Liew Seng Choy yes... my images are obtained using a polarised light microscope at 4x magnification...is it possible to include a scale bar there???
Hi there !
I have no idea the size range.
One way worth trying would be to benchmark it with a known size object within the image.
For example, you may want to benchmark the overall length of your sample.
If the sample looked under the microscope is distributed over a circle, then the diameter could be used as the scale for you to estimate the size.
The fundamental of how the algorithm works is based on the know length compared to the amount of pixel across the measured length.
Another way would be to calculate the size using the magnification. Worst come to worse need to use this method.
Try Google for "calculate the size with magnification scale"
Hope it helps !
Thanks for making a easy to understand video! I was wondering if you could help me measure the distance between two points on a curved path using ImageJ? I have a curved line in one binarized channel 1 and dots along the line in another binarized channel 2. The ROI feature is useful for taking the matrix positions of these features and I found that flattening the image combines my features, but I want to measure how long the path is between points in channel 2 following the path of channel 1. Is there a feature in ImageJ that would do this?
April Peterson It is possible to tell if the scale for both channels are the same?
Is an apple to an apple comparison.
I am not sure of any masking features available. Maybe need to mask the path at Channel 1 onto Channel 2.
Maybe i could help more if i have an example of the above mentioned problem.
Liew Seng Choy the scales across the channels are the same. The images I am using are immuno-histochemical florescence of a tissue spread. I actually have 3 channels which are florescence signals of different proteins. I'll try to describe the example, because I can't upload the data.
Imagine a top down view of a snake (curved line in channel 1), the head is represented by a circle (channel 2) and there are 3 spots along its back (channel 3). I want to measure the relative distance of the spots to the head and to each other.
I hope that makes sense, thanks in advance for any insights or tips!
April Peterson
I wish to help.
THe closest possible solution would be Image > Overlay> To ROI Manager.
Condition is that your file has to be in tiff format.
I have previously made a video about multiple length.
th-cam.com/video/i3pnG-37b8I/w-d-xo.html
IN addition to that, save the file into tiff. WIth all four yellow circles (since it is now circle) which labeled in ROI Manager.
Then, draw straight line between spots for individual distance. Press onto the Measure button in ROI Manager for all length.
Let me know if i managed to solve your problem.
Thank you for this video, it's very helpful. But I want to ask, how were you able to get the known distance to be 01, and how can I apply it to my case. Thanks
Use the scale provide in the image to first calibrate the distance relative to pixel.
Your video is very helpful! thank you!!
Help subscribe to my channel for making it bigger so that I can afford to make more meaningful videos. Thanks.
Thank you very much, this was instructive! It's a shame that the tool won't save the drawn lines associated with the measurements.
Thanks again!
+c0mmanderKeen
I have another video to show the drawn line.
In the same playlist.
LEt me know if it helps.
+Liew Seng Choy I got it to work a while back, thanks!
Could you please let me know if I can automatically calculate multiple lengths in a single image?
I have a distribution of nanorods (approx. 75 in each image) and need to find out the average length.
Thanks.
How about setting up an histogram from data gathered from ImageJ ?
I have another video showing how to set up histogram.
Liew Seng Choy I am stuck at getting that data itself. Right now I am calculating the lengths manually. Please let me know if there is any option in imagej to automatically find out the length of all such rods in an image. If you can provide me your email id I can send you one of the images to clearly point out the problem.
Thanks
Sankalp Verma
I might be able to shed some light on the issue.
Word of advise would be to discuss with your supervisors about this.
The following method is one of among many possibilities to address the problem.
Before i made my personal suggestion, manually getting 75 rods per micrograph isn't really bad if we are talking about maybe 15-20 micrograph images,
A big monitors is all you need. In addition to that, results obtained could be plotted into histogram (th-cam.com/video/2NrT7aI9SJk/w-d-xo.html) i made one video about setting up histogram.
You may of course refer other video in that topic,
It is more convincing once presented into your dissertation as a histogram.
It means, very single strand is manually verified.
Unless you have really huge amount of data to process.
Otherwise,
Using image processing toolbox in MATLAB could be a solution.
(Word of advise would be to consult your supervisor about it)
First, it is not a freeware. That software required license to work.
Second, it takes some few weeks to get around how the programming languages works. Not hard but quite tedious.
Then, only it comes to using the toolbox.
It may takes a few weeks before getting result that might not be promising.
Would be way much feasible to do it manually.
Try use the short cut key (Opt-M) to measure each length, 75rods could be easily done within 45minutes using 21inch monitor !
The problems with writing a program for me is that I'm not very well trained in A.I programming, Problem arise when object overlap !
Try discuss with your supervisor. Maybe there are other solutions.
Liew Seng Choy Thanks for the suggestion. My data set is quite huge so doing it manually would take a lot of time. I have access to MATLAB so would give it a try.
Thanks again.
thanks men! that's exactly what i'm looking for :D
Negra Green
You are welcome !
how did you get the results box to appear?
Try refer minute 3:42 or summary results at 4:32.
Thanks for watching my video.
how to put size of each particle inside SEM image?
Do you mean this?
th-cam.com/video/i3pnG-37b8I/w-d-xo.html
Thanks! Great tutorial! You're an awesome guy!
Welcome subscribe to my channel.
can you measure curved lines
Yes. Right click onto the button for straight line. You may opt for segment line instead of straight line. After you have defined your straight line.
thanks much, i figured it out after the question, but thanks nevertheless
Thank you.. it helped me a lot!
+Muthu Vignesh
You are welcome.
Kindly subscribe to my channel for more helpful videos.
thanks alot for the tutorial, its a life saver
Help subscribe to my channel for making it bigger so that I can afford to make more meaningful videos. Thanks.
Thanks a lot greetings from Tureky
Optonom Scientific
Thank you !
You're awesome! Thank you very much!
=)
Thanks a lot, this is very helpful
=)
Thank you so much nice explanation.
Help subscribe to my channel for making it bigger so that I can afford to make more meaningful videos. Thanks.
Thank you for uploading video..Its nice
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thanks. this is what i need.
+Junaedy Yunus
You are welcome.
Kindly subscribe to my channel for more helpful videos to come....
Thank you man! you helped a lot!
You are welcome. Visit my channel again and see how it has grown after so long later. It can now receive super thanks for each videos.
Thanks, your tutorial its very useful
Yay… visit my channel again for more videos.
thanks a lot that video really helps
Good to know that it is useful.
Thank you for watching the video !
thank you so much....so
helpful..
Welcome to visit my channel again.
Exactly what I wanted to know. Audio quality could be better though. :)
Thank you.
Trying my very best to get a quality microphone in my future video.
The one I had was the one I could afford back then while I was doing my postgraduate study.
Hope by very soon I can have good quality voice video to share and to help many more.
Thanks! It was very helpful!
Help subscribe to my channel for making it bigger so that I can afford to make more meaningful videos. Thanks.
thank you it really helped!
Thank you. Subscribe to my channel for more videos.
Thank you so much!
Good to know that it helps.
Muchas gracias por el tutorial
+Josefina Diez
You are welcome !
Kindly subscribe to my channel for more videos.
extremely useful
Thank you.
Good to know it helps.
Kindly subscribe to my channel for more others video.
Appreciate your thumb up.
well done, thanks!
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Good tutorial!
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thank you very much! :)
You are welcome. Visit my channel again for more videos. My video now can receive super thanks.
Good to know that.
Thankyou so much.... :))
+Aarti Sharma
You are welcome !
Kindly subscribe to my channel for more helpful videos to come....
thank you so much
Help subscribe to my channel.
You can also considder using the easy to use tool: www.imagemeasurement.com
Good to know.
Thank you.
himadri
Good to know you like it.
Welcome.
Thanks a lot
Welcome.
I have another video for getting datas into histogram in Excel environment.
th-cam.com/video/0DMd4jxYi7A/w-d-xo.html
Liew Seng Choy
it was good but no sound
Thanks
Subscribe to my channel for more videos.
Thanks much"""
Welcome to visit my channel again. It can now receive super thanks.
thanks..
You are welcome. Visit my channel again for more variety of videos热.