Demonstration of using ImageJ to measure the size of alginate beads from pictures taken under stereomicroscope. A scaled slide with a 2mm x 2mm grid is used for a reference.
Thank you very much for the tutorial. Can you make a tutorial on how to measure the length and diameter (for example images of tree root) of many images at once?
Hey Dan, Thanks for this informative video, However I am still a bit confused for instance I need to measure the diameter of one Zone of inhibition in mm, how should I switch to mm ?
Hi! Thanks for this video! It was exactly what I needed to know. However, I wonder if you know if you have to have all the images open in image j before you press global? Or will it default to that scale in subsequent pictures you open, until you click to remove scale?
This is great. Very informative! My question is if you can set the scale for pictures with different pixels or they all have to be the exact same pixels from original file? I have different images with different pixels, and one image of a scale (index card) to use for measurement. Thanks in advance!
No the scale can be the same no matter what the file resolution is --- however, if you have different # of pixels, its likely that 1 pixel in one file does not correspond to the same physical measurement in the other file. However you can correct for that mathematically if you know what was going on in the two pictures. For example: if you took a picture of your scale at 400x400 resolution and then calculated the scale to be 1 pixel = 10 um. Then if all you do is change the number of pixels that are saved in the camera - so that your picture you want to measure is 1600x1600 pixels, then for that image the scale would be 1 pixel = 0.25 um. However if you change lenses or focal length or anything else, then your scale is no longer known.
Hey all out there, great video to describe how to use the tool. But I have a task I have no solution for at the moment. For my analysis I want to measure the diameter of small spheres by setting three points on the edge of the spheres and then it should be possible draw a circle out of it. Does someone know, how and with which program this should be possible? Thanks in advance!
Sir will you help me for some image processing, related to trichome (hair like structure on leaf) counting ? I am trying but not getting proper results.
Sphericity seems like a 3D measurement, which we cannot do with this technique because we are reducing it down to 2D. You can calculate circularity from measurements made in ImageJ. There are several different mathematical ways to do that - you would have to choose the one you feel works best for you
You can use the "point" / multipoint tool --- next to the line tool is the angle tool and then next to that is the point tool. When you click to make a point and select "Measure (control-m), it will give you the X,Y coordinates of the point. You can click to make a lot of points and then measure them all at once and it will list them all.
@@danp444 Thank you very much. I have been using this command, it is taking a lot of time as I have on average 200 particles in a single image and need to process a large number of such images. So is there any shortcut to find coordinates? Regards.
i have picture in which i have a sample with known length and there are humps in that sample. its a 2d picutre of sample. i want to cover its humps and want to put on xy-graph to measure its amplitude at various points.. i dont know how to do
I have never been able to understand what means for every record (i.e. n.3) Area, Min, Max and Mean. Is it perhaps connected to the bounding rectangle of the line?
Min, Max, and mean are related to the color (grayscale) of either the line or the box. The area is meaningless unless you have selected a 2D shape -- it is the area in pixels or whatever distance if you set in the scale
hi! thank you for your help. However, each time i set a scale, once i open the window, it goes back to a random number. I am unable to keep the scale. what could be the problem and has anyone else faced the same issue?
Then I am not really sure...I don't think I've had that problem. The best way to mitigate that is just write down the pixels/mm and measure in pixels and do the math yourself I guess.
Hi Dan. thank you for this great video, very informative! for some reason its not working for me. I measure the diameter one by one and then I look at the results but they appear as one line only, maybe measuring the last diameter I drew. Have no idea how to solve this.... any suggestions? thanks!
That's what I need, It's very succinct, unlike other videos. Thank you, sir! Wish you all the best.
Wow, I didn't even know about this software until I saw your video. This is going to be amazing for prototypes! Thanks so much for your help!
Thank you so much! It has been very helpful for my master's thesis on dental anthropology and dental wear in human fossils from the chalcolithic.
Assalamualaikum I am watching this right now very informative videos easy to understand and performe calculations Keep sharing more Videos Thank You 😊
I am really grateful for the informative video.
Simple and clear description. Thanks :)
Thanks Dr. Puperi
It's so helpful for me
Excellent video on image j i have ever seen
you saved my life and final year thesis
Thanks for the video. It is helpful
Thank you very much for the tutorial. Can you make a tutorial on how to measure the length and diameter (for example images of tree root) of many images at once?
It was very helpful for my master's study
Thank you so much!
Hey Dan, Thanks for this informative video, However I am still a bit confused for instance I need to measure the diameter of one Zone of inhibition in mm, how should I switch to mm ?
Thanks for this video, please how do I get the known distance for my own image
Hi! Thanks for this video! It was exactly what I needed to know. However, I wonder if you know if you have to have all the images open in image j before you press global? Or will it default to that scale in subsequent pictures you open, until you click to remove scale?
If you select global, it will apply that scale to all subsequent pictures you open.
This is great. Very informative! My question is if you can set the scale for pictures with different pixels or they all have to be the exact same pixels from original file? I have different images with different pixels, and one image of a scale (index card) to use for measurement. Thanks in advance!
No the scale can be the same no matter what the file resolution is --- however, if you have different # of pixels, its likely that 1 pixel in one file does not correspond to the same physical measurement in the other file. However you can correct for that mathematically if you know what was going on in the two pictures. For example: if you took a picture of your scale at 400x400 resolution and then calculated the scale to be 1 pixel = 10 um. Then if all you do is change the number of pixels that are saved in the camera - so that your picture you want to measure is 1600x1600 pixels, then for that image the scale would be 1 pixel = 0.25 um. However if you change lenses or focal length or anything else, then your scale is no longer known.
Hi Dan, Thanks for the video. Is it possible to find the centre of a circle from Image J? kindly help
Hey all out there,
great video to describe how to use the tool.
But I have a task I have no solution for at the moment.
For my analysis I want to measure the diameter of small spheres by setting three points on the edge of the spheres and then it should be possible draw a circle out of it. Does someone know, how and with which program this should be possible?
Thanks in advance!
Hi! For this to work properly, should my images all be the same in size? Will that affect my results?
How can be find interplaner spacing from TEM?
Muchas gracias
Can we use it for pancreas hypertrophy after h&e staining
Does imagej allow you to label the measurements? or do i need to do that in a separate program?
Sir will you help me for some image processing, related to trichome (hair like structure on leaf) counting ? I am trying but not getting proper results.
Thanks. How can I measure the sphericity of particles?
Sphericity seems like a 3D measurement, which we cannot do with this technique because we are reducing it down to 2D. You can calculate circularity from measurements made in ImageJ. There are several different mathematical ways to do that - you would have to choose the one you feel works best for you
Thank you very much.
Is it possible to measure x and y coordinates of particles?
You can use the "point" / multipoint tool --- next to the line tool is the angle tool and then next to that is the point tool. When you click to make a point and select "Measure (control-m), it will give you the X,Y coordinates of the point. You can click to make a lot of points and then measure them all at once and it will list them all.
@@danp444 Thank you very much. I have been using this command, it is taking a lot of time as I have on average 200 particles in a single image and need to process a large number of such images. So is there any shortcut to find coordinates?
Regards.
may i ask, how did you know the actual size is 2mm?
i have picture in which i have a sample with known length and there are humps in that sample. its a 2d picutre of sample. i want to cover its humps and want to put on xy-graph to measure its amplitude at various points.. i dont know how to do
I have never been able to understand what means for every record (i.e. n.3) Area, Min, Max and Mean. Is it perhaps connected to the bounding rectangle of the line?
Min, Max, and mean are related to the color (grayscale) of either the line or the box. The area is meaningless unless you have selected a 2D shape -- it is the area in pixels or whatever distance if you set in the scale
Were those images taken at the same distance? Otherwise how would you compare your reference of 2 mm to the circles?
how to measure the length if the particle is not in spherical shape?
Dr sb what about nanomeasurements. I have sem image of colloidal silver nanoparticles
let me know if you know the answer please
Great!! But how if i didn't know the actual size in mm?
i want to measure bone volume area from X ray imaging
You have to have something that you know the actual size of in mm, or you can't set a scale.
hi! thank you for your help. However, each time i set a scale, once i open the window, it goes back to a random number. I am unable to keep the scale. what could be the problem and has anyone else faced the same issue?
Are you making sure "Global" is checked in the set scale dialog?
@@danpuperi4194 yes it is checked. Even in the same image, the moment i close the scale and reopens, it resets...
@@danpuperi4194 thank you for responding!
Then I am not really sure...I don't think I've had that problem. The best way to mitigate that is just write down the pixels/mm and measure in pixels and do the math yourself I guess.
@@swansonp8582 Hello. did you manage to solve this problem? I'm trying to resolve this issue too but with no luck. suggestions? thanks!
Hi Dan. thank you for this great video, very informative! for some reason its not working for me. I measure the diameter one by one and then I look at the results but they appear as one line only, maybe measuring the last diameter I drew. Have no idea how to solve this.... any suggestions? thanks!
Hard to say...but it will measure the line you have currently selected, so make sure you have the one selected you want.
Did you say, "Hi, everybody," or, "Hi, buddy"...?
Sir, how can I download this Fiji app please tell me the link of this software.
imagej.net/Fiji/Downloads
it does not work with the new version, the scale reset every move I make
mine too. have you managed to solve the problem?
Why 2 in known distance