How to measure leaf area in ImageJ program

This is the Reference of the ImageJ pro.
Schneider, C.A.; W.S. Rasband, and K.W. Eliceiri. (2012). NIH Image to ImageJ 25 years of image analysis. Nature methods 9(7): 671-675.
Keywords: Eggplant leaves, ruler, ImageJ
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Nashwan Yousif Eido
Lecturer in Plant Physiology
Horticulture Department,
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[@nikospapson9412]

Tommorow I have to measure the LA of 180 plants. This is so useful thanks!

[@Rosewarmth.]

You have saved my life! Thank you lol

[@MArifinDobson]

Gosh this is awesome man

[@casilon6955]

Useful tip, thanks

[@andrewmcc6935]

Hold shift when you're drawing a line on ImageJ. It will only make the line horizontal or vertical, no faffing about with angles

[@luxubulaxaba]

Thank you, friend!

[@azaddoski1082]

well done

[@n89n-na1sh9wa8n]

Thanks

[@appiahkubi1203]

Please I'm working on my project and I'm supposed to find the area that has been consumed by insect herbivores on some leaf samples but I'm struggling to perform it in imagej .. please can you kindly help me

[@nikolatesla9734]

The area result is 87.890 for first leaf. What does it mean? Is it mean the area of first leaf is 87.890 cm^3 and what are the meanings of "mean" "min" "max"? What do they show? Can you explain. Thanks.

[@n89n-na1sh9wa8n]

It means it's in square centimeters.
Mean = sum. of all leaves on the number of leaves (10).
Min = is the minimum area of the leaves. And which one has the lowest No.
Max = is the maximum area of the leaves. Which one has the largest No.

[@salah7070]

Thanks for this useful video. I follow it to measure leaves size but some of them give me NAN so the mean becomes NAN, do you have any idea why ?

[@n89n-na1sh9wa8n]

I think the background should be white and put the leaves on it and give them enough space between each leaf.

[@ayeshakhalid9832]

Can you please tell how to measure the area of a subsection of an image ? e.g if we want to count cells from certain area in an image and want to determine that

[@n89n-na1sh9wa8n]

@@ayeshakhalid9832
I think this pro. can not count cell number, but u should use Microscopic for cell counts

[@Paoloforattini96]

Hello, can you apply this to a database of pictures? Thanks

[@n89n-na1sh9wa8n]

Which type of pictures?

[@raghavdadhich9774]

Hi, how can I use pendent drop surface tension plugin for image j. Can you help me please.

[@n89n-na1sh9wa8n]

Hi, I don't know what does it means

[@Daisychain48]

Does this refer to the surface area of one or both sides of the leaf?

[@n89n-na1sh9wa8n]

Of course for both sides

[@habtamugetnet2778]

how about one side area?

[@watchurwa]

what if there are holes in the leaves .. these are not outlined yellow.. are they still excluded?

[@n89n-na1sh9wa8n]

You can check it in another way with the same pro.

[@watchurwa]

I want to exclude the holes from my area measurement..

[@watchurwa]

@@n89n-na1sh9wa8n Thanks for your answer

[@watchurwa]

@@n89n-na1sh9wa8n so it is automatically excluded when in the analyse particle tab the include holes is not ticked?

[@watchurwa]

@@n89n-na1sh9wa8n can you please answer?

[@harshasg412]

Hi brother, can we measure the same in black background...?

[@n89n-na1sh9wa8n]

Hi, I think nope, because the black background affects with the red color

[@prajaktagaikwad2914]

how did u find distance in pixels in set scale

[@n89n-na1sh9wa8n]

U don't need a pixels, Just measure via ruler that you want and set in known distance

[@prajaktagaikwad2914]

@@n89n-na1sh9wa8n ok thanks

[@HAN_Dynamic]

I use matlab. How can I do it in Matlab? Thanks.

[@n89n-na1sh9wa8n]

Hi, Sorry I never use Matlab