ImageJ Analysis: Length Measurement, Area Measurement and Thresholding
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- เผยแพร่เมื่อ 2 ต.ค. 2017
- In this ImageJ tutorial basic analysis of any image like length and area measurement are demonstrated both by manual and thresholding process.
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Instructor: Santanu Mandal
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Thank you so much from Buenos Aires, Argentina. My students and I were watching your video. The cadence of your voice is perfect for people who is not a native English speakers. Keep doing videos for research community.
You are most welcome :)
I am using Image J to analyze herbivory in my honours thesis, and this tutorial really helped me understand how to use it! You are a life saver, thank you!
Thanks for the tutorial, has now allowed me to analyse bubbles in a foam, the threshold is very difficult but your demonstration on how to do it manually has helped
Thank you so much for this video! It's hard to find straightforward explanations for ImageJ.
I was searching for such calibration since many days.. Thank you so much you solved my query!!
Thanks a lot. Was exactly what i was looking for. I had no idea how to start using it but this video had all the info i needed and i was able to tweak the steps for my study. Lifesaving! So grateful😄😄
So helpful!! Thank you so much! I could easily apply the methods on my data and had great effects, the video was an amazing help! :)
Thank you for your video and tutorial! Very friendly for new users!
This video was super helpful. I'm trying to determine if ImageJ will work for my project and it seems that it will. Thanks for making this video.
Thank you for your help. Very slow and well explained features. Thanks again.
Thanks from Mexico. It really helped me a lot. It's clear and useful for beginners in this software
thanks for the team who made this video in a fruitful way. come up with many more useful videos for the research community. All the best for SMS team.
Thank you so much for encouraging words!!!
Thanks for the good work. It's my first time trying these analysis, videos just like yours helps a lot!
Thank you from Indonesia. This video help me a lot!
Very useful, Thanks for providing such things
thanks for all of your videos, very good explanation, slow but you have explained all the details :)
Thank you sir. Really appreciating work.
Great video!!! Thank you for the effort.
Very rich presentation for me as a beginner. Thank you very much
Thanks for the tutorial. It is a life and time saver. Kudos 🎉
Thank you very much, your work is so precious...
Thank you very much.I could learn plotting histograms using this video
Thank you santanu mandal!!!
From,
Saurabh Mandal😊
very informative video, thanks a lot for such an easy way explanation
I am so delighted to watch this video. Now my problem is solved🙏🙏
This is a very good and helpful video. Thanks!
Also I noticed you are recording this at 1am :D
Thank you very much for this tutorial 🙂
Simple and awesome......:)
Great video....it's really helpful
So helpful!! Thanks so much!!
Thank you very much. This video saves me a lot.
Awesome video. Very helpful.
BIG BIG thanks for the great video!!!
Thank you for your video.. Very usefull for me
nice lecture..pleas upload more videas regarding image J
its really helpful sir thanks a lot
thx for the informative vid. although there were times where i had to rerun with your accent a bit thick at times. perhaps add subtitles for future vids?
Thank You for this. Very helpful.
Thank you for introducing this cool software.
How to draw not a straight line but a curve? I have fibres i want to measure the lenght of but a line is not the tool i need for this.
Thank you very much! Super helpful!
Thank you very much Sir. It is very nice and informative lecture for the learners.
Great video! thank u. Quick question though- what are the units for the area? if I clibrated a scale of micrometers, than is is um^2 or some other defalt units?
Thank you for explain but I have question about thresholding with ImageJ...how I can devise histogramm of grey scale image in different ranges from 0 to 255 for your image J for exemple..
Thank you
best vidio ind the word my best youtuber :D
Thank you. really helpful tutorial
Thanks a lot for your explanation Sir
Excelente. Muchas gracias.
very helpfull, thanks too much
Helpful. pretty nice presentation
Thank you sir, very useful video.
Thank you very much! It is pretty helpful for freshmen. May I ask a question? Why it is required converting to 8-bit before using image?
can we use imageJ for measurement of diameter of cells e.g. bacterial cells from TEM images??
Thank you so much sir 💐 for making this so simple for me... You are a great teacher.... ❤
really helped me.
Explanation super sir
Is there anyway to automate finding distance between points? I am trying to find the distance between neighbor atoms in a lattice from a TEM image but it would be cumbersome to do it one by one.
Awesome, thank you so much.
thanks for your contribution
Thank you Sir for the video
Thanks. What is the area value showing in output when we measure the distance between two particles. Highly appreciated for your help.
thank you sir for your video
Learned alot. By any change do you know how to calculate collagen fragmentation index of superficial dermis?
Thank you so Much for This.
This is very helpful! Thanks for your video!!
Glad it was helpful!
SMS, we need to determine the area of a JPEG image and then within that area an anomaly.... that way we can compute the percent that the anomaly is of the total area. Do you recommend ImageJ or jiji ImageJ? We cant calibrate or determine the area of size within the image, that is why we need to determine the percent. The 1st area is essentially a circle the 2nd inside area is somewhat of a triangle. Thanks in advance.
Thanks for the video
Thank you for the video. I am using Image J for analysing my bubbles (from Foam). Since the bubbles are see through, I need to use the manual method for selecting the bubbles. However , this is taking a lot of time and some cases it considers one big bubble as two and calculates the area. What is the solution for this? Though I set the scale initially to cm, it displays results in area percentage. In this case what would be the unit of my results? Please help.
hey i have also the same situation. did you solve this problem?
I wish the audio were louder, and I wish you spoke faster. Other than this, an excellent video, and I thank you for sharing it.
Hi, Great tutorial video ! I have a question, when I clicked the particle size analyzer, a small window popped up and showed a table of area, minimum, maximum, and mean. Do you know what is minimum, maximum, and mean stands for the particle with an abstract shape (its shape is sometimes like rod and sometime like oval) ? (i'm working with micro fibril cellulose/ MFC particle). Im not sure that minimum and maximum stand for width and length, because the value is much larger than area. I hope someone answer my questions. Thank you so much !
First of all thank you. Coming to your question, min, max and mean represent the 'gray value' of the particular selection. Gray value '0' means complete black and '255' means complete white. Hence these things are not related to shape, rather related to color. One more thing is that you are getting - 'area', 'min', 'max' as default in 'result' pop up. But you can set those parameters or add more according to your requirement by going 'Analyze'--> 'Set Measurement'.
Thank you so much :D
so helpful, ty.
Thank you for your sharing
Thanks for the video =)
great video! why you need to convert your image to 8bit?
Nicely explained
if my images was more than 100 can i measure the length and width automatically instead of select the images manual?
please upload more videos regarding to foliar anatomy.... (cells length and width measurement)
Thank you very much, for your tutorial.
I was wondering how can use the threshold if I have holes onto a surface area that I want to measure? It feels like they break my total area in small pieces. I was thinking to take about 5 measurement of it manually at this point and use a standard deviation, could that work?
Yes. It should work perfectly.
Why do you convert image into 8bit mode? What happens if you retain RGB mode? Thanks
Very useful
your Video helped me a lot thank you but plz try to make your voice more clearly =)
Hi professor. Great video! I will appreciate so much if you can clarify my doubt, it is about the result of the mean value obtained by the thresholding method. The summarized results showed 2851.485nm^2 +/- 1217nm^2. So, is it possible to say that the grain size of your sample is: 2851.485^0.5 +1217^0.5 = 53.40 nm +/- 34.89 nm ?? Thanks for your time
that would obviously depend on the shape you want to assume. Most particles shapes are approached using spheres so you would need to find the diameter of a sphere with given cross sectional area.. if the shape is very irregular i would either take an average of different lines through the particle or choose a strategy (like e.g. finding the maximum Feret diameter)
Thank you very much
Hi, this is very helpful and easy to follow. Thank you very much. Is there a way to measure the selected rectangular area so that the same area of interest can be kept uniform for multiple images? or if we select the rectangular area in one image and are analyzing a stack of images, will that same rectangular area selection will apply to all?
Hello, I had the same question as that of yours but unfortunately, I was unable to find any videos regarding having a constant rectangular area and using it for all analysis. Please let me know if you figured out a way. It would help me greatly! Thanks!
hello, why do we have to convert the image into 8bit? just curios
Can you install it on android phone
Sir will you help me for some image processing, related to trichome (hair like structure on leaf) counting ?
Sir, could you help me, how can I measure 0.0625 sq. mm area on an image by using 0.25 mm scale bar. Thank you
Thank you Dr. Kundi
how to get a scale bar for any digital clinical photo of facial structures.
Can we calculate the sphericity of the particles please help
can anyone help me in finding fiber dispersion and orientation in FRC by using IMAGE J analysis??
sir, i have 2 questions. 1. why you convert image to 8 bit?
2.after measuring length of the particle, i want to write text in the image. i used A symbol and i write length unit. once i write i cant edit. can you help me about this
Nice video, more fps would be great though.
I dont have the scale value when i open my picture like in your case (300nm). what should i do?
Abdul Salam you can use an image measured from the same camera and magnification to set a scale. In biology people often use hemocytometer to capture an image and set a scale
hi is there any way I could contact you guys
wow sir .....very easy to use ImajeJ after your tutorial.......one question sir can we get the composition of composite materials (eg: Nickel Ferrite and carbon) from ImageJ?
Thank you. You have to do XRD/EDX for composition analysis. Using ImageJ ,we don't think its possible!!
Thank u sir
hi may I know where is the next video on threshold in image j?
how to measure thickness?
Thanks.
But what area is measured when you measured the inter-particles distances?
There is no area. It is a linear distance.
When I scanned my leaf, I forgot to put ruler beside it so I cannot do set scale. what do you think I should do?
If you know the length of any part of the leaf ( which you can measure manually after scanning using a scale), just draw a straight line over this know length. Using set scale, give it the value, which is measure manually. This process will not be 100% accurate, but it will work.