DNA Replication in Prokaryotes | Initiation

In this video we have discussed the Initiation part of DNA replication in Prokaryotes where the enzymes and proteins attach to the DAR sequence and initiate the process of unwinding . The unwinding is done by Dna A protein and then other proteins accompany the initiation process.
The Proteins involved in process are :
Dna A protein : ADP and ATP Bound.
Dna B : The Helicase enzyme which breaks the Hydrogen bonds.
Dna C : Helicase Loader.
Dna G : Primase enzyme.
Previous Video : • DNA Replication | Prok...

[@Emilia-we2dc]

Literally the BEST video on this subject. Summarized everything that was in the textbook way more visually. Thank you!!!!

[@hussainbiology]

thanks for appreciation....Glad it helps

[@takemein]

You can also check here.
th-cam.com/video/zoJN-PVdeqc/w-d-xo.html

[@you-gn4eh]

Which book?

[@lalalalalala-cb8bn]

Which book?? Please do tell

[@SONALI_1]

Hussain biology is best as far I have watched videos ....he explains everything logically...whenever I have viva I visit his channel to clear my concepts just overnight ...its really helpful...I m again here for learning for my exam that is day after tomorrow 🙃.
THANK YOU SO MUCH SIR 💓

[@hussainbiology]

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[@nigelsimbarashe6433]

i like this, pure accurate dispensing of molecular biology knowledge. Thank you very much Sir

[@hussainbiology]

thanks for appreciation..Glad to know that it helps✌️

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⭐⭐⭐⭐⭐ God bless you sir and shine like a star in the sky of knowledge......

[@NaDee-mh2po]

I have a test for this on Thursday. Thank you for making it easier to understand.

[@hussainbiology]

Thanks Daniel for appreciation...

[@takemein]

You can also check here.
th-cam.com/video/zoJN-PVdeqc/w-d-xo.html

[@shivaniojha4344]

Thank you sir for making such nice videos
Your videos let us understand topics clearly in very less time..
Thank you so much

[@poosayfresh8880]

Best detailed video on entire TH-cam

[@snehaldesai4423]

There are 5 copies of 9 mer sequence

[@rohanavinashphatak2747]

No words yar to express your marvelous teaching. I just love the way u proceed step by step. The most appreciative part is u directly come on the point rather than taking curves n turns and landing up confusing the audience unlike shomu does. Just excellent 👌👌👌👌. God bless. Live long

[@hussainbiology]

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Thanks u so much
U make every concept very easy

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Thanks buddy for appreciation...Glad to know that it helps

[@nouriakhan8656]

MashAllah ...hats off to u .....brilliant lecture delivered ❤.....
Teach pharmacology as well...😢💔
will be thankful to u 😊...

[@hussainbiology]

Thanks for appreciation.. Glad it helps..
InshaAllah Pharmacology soom

[@nouriakhan8656]

JazakAllah ...

[@nifatjan7458]

Clear cut concepts....great explanation....thnx sir

[@hussainbiology]

thanks for appreciation....Glad to know that it helps

[@bhatjas1820]

yes.. they do... very much!!! great efforts👌

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you are deserve more and more subscribers bro

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Thank you so much sir, its really helpful to clear the concepts.

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[@ayeshaali9392]

today its my paper & am here to get the summarized knowledge.
جزاك الله

[@hussainbiology]

Alhamdulillah...I am really glad to know that..... All the best

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Thank u sooo much ,this makes my day (respect from kashmir)👍

[@hussainbiology]

Thanks Najma for appreciation.....Glad to know that it helps...( from Kashmir too )

[@barashakalita7772]

Does the four 9 mer sequence lies on the downstream of replication recognition sequence

[@hamihamro]

Finest video on the topic in TH-cam ❤️

[@hussainbiology]

Appreciate that

[@debaswapnaray3295]

But sir pehle dnaB bind karta he uske baad ssb protein nhi toh helicase kam nhi karega agar pehle ssb lagade toh...otherwise its a best explaination video

[@hussainbiology]

Thanks for ur feedback and that is right what u say .
I too have mentioned the same
@ 3:59
Thanks

[@saikumargsaikumar8657]

I really like each and every video in Hussain biology ....concepts are really crystal clear.....keep it up

[@Rishab1702]

Wow sir u r an artist who explain it's knowledge in terms of art. Tysm for explaining this topic

[@hussainbiology]

Thanks Rishab for appreciation....Glad to know that it helps

[@red8527]

Your graphics are spot on. 🔥

[@hussainbiology]

thanks buddy

[@anamikagarg9703]

This is so helpful, Thanks much for making this.

[@hussainbiology]

thanks for appreciation

[@bidhisingh9982]

u r awesome my bro . thanks.....

[@nishanisharma6289]

👍Well explained Sir. Thank you so Muchhhhh

[@hussainbiology]

thanks for appreciation..Glad it helps ✌️

[@ammaramehak9473]

Nice videos for post graduated students thanx

[@hussainbiology]

thanks Ammara for appreciation.... Glad to know that it helps

[@kaavyasurianarayanan8247]

Woaahh!! You are sooo good sir!! Thank you so soo much!! Really helped me!!

[@hussainbiology]

thanks Kaavya for appreciation.
Glad to know that it helps

[@shivanithakur1372]

No doubt you videos are highly informative and great to clear concepts only problm what I found is clearity of voice.. Otherwise video is with great information

[@hussainbiology]

will improve

[@WasimAkram-tk9sh]

hats up sir
may allah blessed u sir

[@purnimaash2063]

Your videos hlp me a lot, thnq bro.. keep it up

[@hussainbiology]

thanks Purnima for appreciation

[@WasimAkram-tk9sh]

sir we r waiting 4ur next lectures elongation n termination process ...Insha -Allah it will b very soon

[@hussainbiology]

InshaAllah Elongation and termination process coming up on following Monday

[@ManpreetSingh-ds4nn]

What a nice explained sir

[@hussainbiology]

Thanks Manpreet for appreciation....Keep sharing and supporting

[@romankhan2935]

Where did you learn this bro.

[@hussainbiology]

from books and internet

[@romankhan2935]

@@hussainbiology which book?

[@nithyapriya.n1047]

Very well explained sir...Thank you..

[@hussainbiology]

thanks for appreciation

[@mitynonang1372]

What does mers mean sir plz reply?????

[@hussainbiology]

Mer means part ( in Greek ).. like hexa-mer ( six part when we have Length of six oligonucleotides)

[@ShayaqUlAbeerRasool]

Hussain You are making amazing content. Are you Kashmiri?

[@hussainbiology]

thanks brother... Yes i am Kashmiri

[@aashmagyawali6331]

Thank you so much for your effort.. clearly understood

[@hussainbiology]

thanks for appreciation..Glad it helps ✌️

[@leapotocnik7170]

THANK YOU!!!

[@hussainbiology]

You're welcome!

[@prakritisamnotra6671]

Thankyou so much for making this too easy

[@hussainbiology]

thanks for appreciation..Glad to know that it helps ✌️

[@hafizmuhammadrehan074]

Ahaaa kea bat ha🤗🤗🤗🤗

[@girikowshik3724]

Very clear explanation sir

[@hussainbiology]

thanks for appreciation

[@sidrakadhim5102]

Thank u so much, it is really helpful !!!

[@hussainbiology]

thanks for appreciation..Glad it helps

[@akankshabhardwaj2877]

Can you plz tell the name of reference book.....plzzzzz

[@hussainbiology]

i use Lodish and Alberts cell biology

[@akankshabhardwaj2877]

@@hussainbiology thnku😊

[@hussainbiology]

@@akankshabhardwaj2877 My pleasure

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I am a Hussain Biology loyalist✊

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Glad to know that... Lots of love

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Simply supperb

[@bhatsuriya172]

nice bro....!! as usual..👍👍👍

[@hussainbiology]

Thanks Suriya,,,,

[@letstudy2425]

What is the function of DNA pol 1 ...if DNA poly 3directly coming after primase

[@jyotisoni9814]

Hey!
Hope u will reply soon.
I have my mol. Bio exam tomorrow and there is a topic written in my syllabus "structural genes (as +nt in mRNA) - existing gene number by kinetics of RNA driven reaction"
I m not able understand what should i write on this and also not getting it on google.

[@hussainbiology]

Hi Jyoti , have read ur query but a also couldn't find anything related to it.

[@jyotisoni9814]

@@hussainbiology Okay. Is that structure gene ....mean gene structure? In reference to internal organisation what it could be.

[@hussainbiology]

@@jyotisoni9814 Most probably it could be the Structural genes organisation ( structural genes include lac z lac y and lac A genes -operan ) there are several other structural genes but these are the most imp ones

[@jyotisoni9814]

@@hussainbiology okay....tnq so much.

[@salmanfariz1052]

Thank you so much sir.great effort

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Thanks Salman for appreciation

[@tayyabsulehri439]

Good effort

[@Arrahaha]

The best you are.

[@hussainbiology]

thanks for appreciation

[@firdoskhan5580]

I want the link on DNA replication on the Goole site ..... Can u plz provide it.......

[@JulianTheHunter]

Perfect! Just what i needes :)

[@AhmedShadi95]

Excellent!

[@hussainbiology]

thanks for appreciation..Glad to know that it helps ✌️

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👏sir plz... do upload more videos

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Thank you.

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You're welcome....

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Supperb

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thanks for appreciation

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Thankyou so much sir

[@monishas906]

Tq sir pls more and vedios

[@joancynthia9393]

Good.. 🤗

[@hussainbiology]

Thanks Cynthia for appreciation.... Keep sharing and supporting......

[@swarnapravajenajena2803]

Tqq sir. i need dna replication NOTE

[@hussainbiology]

I haven't compiled notes yet.... Thanks for appreciation

[@ridhazainab3225]

are u from kashmir

[@hussainbiology]

Yes Ridha I am Kashmiri ....

[@kavitaprajapati6208]

Sir iska pdf download kese kare

[@manishachougule3883]

Sir, which books do u refer for molecular biology?

[@hussainbiology]

Three Books :
✓ Lodish Molecular Biology
✓ Alberts Molecular Biology
✓ Cooper Molecular Biology

[@sabee.67]

Thaaaaaanks a lot

[@hussainbiology]

Most welcome ✌️

[@СедаГаспарян-д8к]

great

[@hussainbiology]

Thanks for appreciation... Glad to know that it helps

[@avonkumar6301]

Thanks sir

[@hussainbiology]

You are welcome Avon.

[@monishas906]

Sir pls help me for to qualify the csir net .

[@hussainbiology]

Study and do continue watching videos...

[@ammarahmehak9986]

sir plzzzzzzzzzzzzzzzzzzzzzzzz translation in eukaryote and prokaryote post transcription modification , post translationa modification pr video bna dy it's a request my exam is near plz upload video in two or three days

[@hussainbiology]

Ammarah I have already replied to you abt this , currently i am unable to upload videos due to unavailability of high speed internet.

[@stellac4230]

Thank u sm❤️

[@hussainbiology]

You're so welcome!

[@sejalshende6286]

Great explaination. But you should reduce your speed of talking.

[@hussainbiology]

thanks for appreciation...And Surely i will slow down

[@bishnupratapsharma8473]

Sir plz provide PDF also.

[@buriiib7669]

Hey sirr

[@buriiib7669]

Nd ur hlpp sir plzz

[@dailybiz_vlogs9317]

Kya fayda itne mehenge textbooks kharidne ka

[@hussainbiology]

kyu

[@dailybiz_vlogs9317]

@@hussainbiology sir qki yha aapke channel pe jo itne aasani aur aache se smz aa jata h wo books se ni aata

[@hussainbiology]

@@dailybiz_vlogs9317 acha mughe laga shayd mere he lecture mai kami hai✌️✌️✌️btw thanks

[@samikshasaxena731]

Namaste mam!!!!
Samiksha saxena
JV-U/19/3449
BNYS first yr..
8th May 2020
9:03 am

[@satyasantan855]

हिंदी बोलो यार हमलोग भारत के है 😂

[@muskangoyal4030]

sir there are 5 9mer sequences rather than 4...

[@hussainbiology]

yes.. accepted.. there is a site missing

[@maxruphio5515]

Thank you very much Hussain Biology

[@hussainbiology]

Thanks Max for appreciation ....Keep sharing and supporting

[@RohitPant04]

Great explanation, Hussain but doesn't Ori-c sire in prokaryotes contain 9 nucleotide motifs of 5 repeats & not 4 repeats as you mention here?

[@alisalisna2019]

I agree with you! There is for sure 5 repeats of 9-mer motif. Plus at the end of his lecture, he mentioned a DNA pol III holoenzyme, that in fact has three DNA polymerases (not just two!): one of them is working on the leading strand and another two on lagging strand - "trombone model".
Here is some evidence:
pubmed.ncbi.nlm.nih.gov/22157955/
www.ncbi.nlm.nih.gov/pmc/articles/PMC2859602/
th-cam.com/video/sxedBRA18Ro/w-d-xo.html

[@kaavyasurianarayanan8247]

I agree with you!!

[@risingsun9247]

You are right there are 5 R boxes instead of 4

[@risharajkhowa5060]

Yes , theta are 5 9mer repeats

[@RobertJurilj]

No, there are 4 of 9mer repeats in E.coli
He was right

[@kathy-leew3989]

My professor gave us a different approach as to how the dsDNA becomes ssDNA at the 13-mer region. They told us that the ssDNA-binding-domain in the DnaA Protein causes the strand separation and didn’t mention an introduction of topological stress due to the coiling of the DNA around the Protein DnaA.
I find your explanation much more logical. Thank you!

[@vasudevkoundinya252]

thanks yr.. you worth respect in comment section..(at least)

[@hussainbiology]

thanks for appreciation....Glad it helps

[@biologytutelage6960]

The video is good... But i found it quite complex to understand.... It should be more simplified...

[@TheZitface5]

5:05 I think you got your leading/lagging strand messed up

[@hussainbiology]

yes i have a mistake there....

[@Wanderer_priya]

9 mers seq are 5

[@sahibaandherideas2252]

9 mer sequence -5 times

[@dr.muzafarhussain9821]

Nice explanation....make more vedios..

[@hussainbiology]

Thanks for appreciation

[@alfredafriyieasiedu]

Very detailed and understandable, thank you!

[@saibarsha4614]

Thanx sir for solving my doubt through this vdo

[@hussainbiology]

Thanks for appreciation

[@biology_mcqs3037]

👍👍👍👍

[@hussainbiology]

Shukriya boi...

[@riyajiyani3844]

Are these videos enough to study for concept of CSIR NET JRF or GATE biotechnology

[@hussainbiology]

Yes definitively.....

[@armaansidhu3018]

Why there are four 9 mer sequence on ori? Why R3 is not mentioned in the diagram ?

[@hussainbiology]

apologies... certainly there is one ori site missing

[@schoolofscienced.r5816]

Very good 👍

[@ayushijaiswal1183]

This video is so old but still it is the best for this topic. Excellent👏

[@hussainbiology]

Glad you think so!

[@bidhisingh9982]

Ap jeeyo hazaro saaal.

[@hussainbiology]

Thanks Bidhi for ur support...keep sharing and supporting

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youre the man

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Thanku

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Mashallah... Allah apko salamat rakhe...

[@hussainbiology]

Aapko b... ✌️

[@ayeshafatima-lq1qp]

Thank you sir,make more videos on CSIR topics

[@hussainbiology]

Thanks Ayesha for appreciation....Glad to know that it helps.