Serial Dilution method | viable cell count method

Serial dilution is a viable cell count method. In microbiology practical serial dilution method is used to count microorganisms present in any sample.
Visit blog for more details: rbrlifescience.com
Hi, I Am Rahul B Rathod, Welcome to Our TH-cam Channel RBR Life Science
About this video-
friends, In this video tutorial we are going to learn How to make serial dilution practical in the microbiology laboratory thank you so much...
Queries Solved:
1. How to make a serial dilution
2. How to perform serial dilution procedure in microbiology
3. How to calculate serial dilution
4. How to use serial dilution to isolate bacteria from soil
5. How to prepare a serial dilution
#serial dilution
#serial dilutions
#serial dilution method for bacterial culture
#serial dilution calculation
#serial dilution method
#serial dilution in lab
#Viable cell count microbiology
#Viable counting techniques
#Serial dilution technique steps
#Serial dilution protocol
#Viable count method procedure
#serial dilution method
#microbiology count methods
#english
#rbrlifescience

[@plantprotectionscience3646]

So nice

[@simuchelomunkombwerkikbeiv6842]

Finally,well explained

[@deepakkumarpanda9350]

Informative Video with excellent explanation.

[@rbrlifescience]

Thank you Deepak..and stay tuned to watch such more informative videos... 👍😃

[@sandismike3822]

really helpful 👌 👏

[@dilawarkhanpathanniazi1369]

Sir very very good lacture for concept and understanding ☺️🤗 this method ♥️👌

[@rbrlifescience]

Thank you dilawar Khan ji...

[@umamishra7757]

No words😊😊😊

[@latestcraftwithrubab6393]

Finally, find the authentic content

[@rbrlifescience]

Thanks for watching this video...Stay tuned to watch such more videos

[@gangaarach3305]

excellent explanation...thanks..

[@rbrlifescience]

Most welcome and stay tuned for more videos

[@harshadaadgulkar1029]

Nice explanation sir thank you sir for wonder full explanation

[@rbrlifescience]

Thanks harshada

[@asifk8459]

Sir, we say thank u so much sir.i require like this teaching sir . 😊🙏🙏🙏🙏🙏🙏🙏

[@rbrlifescience]

Thank you and please suggest such topics on which I should make videos

[@madhusahu3655]

Very helpful vedio thank you

[@rbrlifescience]

Thank you madhu....let me know other topics on which you want such videos

[@Sarojkumar-oq4hn]

Realy so nice explanation 😮

[@rbrlifescience]

Glad you liked it

[@malathibalu7467]

Very good explanation sir

[@rbrlifescience]

Thank you madam and please suggest any topics on which I should make videos

[@simantikasadhukhan6522]

Best video on CFU❤

[@rbrlifescience]

Thank You. ....and please suggest any topics on which I should make videos

[@lexusl849]

Great knowledge

[@rbrlifescience]

Thank You

[@gamecaptain1632]

Very helpful videos .... please make video on molecular technics ...PCR , RT-PCR, Nano drops , Sequencing, Electrophoresis,ELISA, Chromatography, spectrophotometry

[@rbrlifescience]

Thank you for suggestions...I will definitely make videos on molecular techniques

[@sandip45882]

Perfect 👍

[@rbrlifescience]

Thanks 👍

[@benibuntu2052]

❤❤❤ ⚘️🌷⚘️...Thank you...🙏🙏😊😊😊👍👍👍

[@rbrlifescience]

Most welcome

[@deepssih7787]

Thankyou so much sir for making my concept very clear...

[@rbrlifescience]

Most welcome Deep ...Please also suggest some concepts on which I should make videos

[@abdulmajidal-yafei2793]

thank you

[@rbrlifescience]

most welcome

[@arshiaamjad9220]

very nice

[@rbrlifescience]

Thank you

[@satyaprabhu6207]

K I got it!

[@rafiqulislam3060]

Thanks

[@rbrlifescience]

Welcome

[@aliyuabubakar-kt9wf]

Pls sir do you have any video on complete method for culturing bacteria

[@rbrlifescience]

Please explain in detail

[@aerospaceplayer5811]

Good sir from pakistan

[@rbrlifescience]

Most Welcome

[@salettedepintosouza5455]

if your trying to determine the bacterial resistance to a particular antibiotic using serial dilution and total viable count what are the acceptable counts and what are not the acceptable counts to say whether or not bacterial resistance is taking place?

[@rbrlifescience]

Please go through this wic.oregonstate.edu/microbiology-writing-guide-presenting-data

[@dieudonnenyamaifofe9646]

What about if the 2 plates from 2 different dilution ( 10^2 and 10^3 for example) have colonies within the range of 30-300. What dilution should be consider to calculate the CFU/mL in the undiluted sample

[@MorganHunte]

In theory - If you did a perfect serial dilution, the calculations that you do based on the colonies you've counted on these two plates should give you the same CFU/ mL. If the numbers are drastically different, there was likely an issue with the dilution scheme, with the calculations, or with the environment (i.e wrong with the agar). In the interest of time, I would choose to count the plate with fewer colonies. Hope this helps.

[@rbrlifescience]

Yes thats very correct

[@shaftasyed6244]

What about other plates in which we got countable growth how can we include those in calculation and we usually take duplicate or triplicate how can we calculate in such case

[@rbrlifescience]

suppose you have plated 0.1 ml of sample of 10^3, 10^4, 10^5 dilution on three different plates. suppose you got 100 colony on 10^3 dilution, 11 colony on 10^4 dilution, 2 colony on 10^3 dilution,
first conver the count cfu/ 0.1 ml to cfu/ml
10^3 (100 colonies) = 100 x 10^3 cfu/ 0.1 ml = 100 x 10^4 cfu/ ml
10^4, (11 colonies) = 11 x 10^4 cfu/ 0.1 ml = 11 x 10^5 cfu/ ml
10^5 (2 colonies) = 2 x 10^5 cfu/ 0.1 ml = 2 x 10^6 cfu/ ml
now convert these 3 result to one specific dilution result
10^3 (100 colonies) = 100 x 10^4 cfu/ ml = 100 x 10^4 cfu/ml
10^4, (11 colonies) = 11 x 10^5 cfu/ ml = 110 x 10^4 cfu/ ml
10^5 (2 colonies) = 2 x 10^6 cfu/ ml = 200 x 10^4 cfu/ ml
then average it (100+110+200)/3 = 136.66 x 10^4 cfu/ ml
136.66 x 10^4 cfu/ ml present in your original undiluted sample
In the case of duplicate or triplicate samples, just average the results of specific dilution.

[@aitakil7294]

Après on fait la moyenne ?

[@atiqaanis4098]

How can we will perform serial dilution method for tonsils sample after tonsillectomy for bacteria identification?

[@rbrlifescience]

please refer medical procedure, I am explained only general procedure

[@hamidahmadmalik572]

How to know the number of bacteria in original soil sample... We added 1 g in 50 ml... How to know the number of bacteria from that 1 g of soil ...

[@rbrlifescience]

Suppose you added 1 gm soil in 50 ml of Distilled water/ saline
Now you are taking 1 ml of this sample and serially diluting it.
You took 0.1 sample of each diluted tube and plated it.
Suppose after plating 0.1 ml of sample of tube having dilution 10^-3 (ten power minus three ) you got 10 Colonies.
Now it's 10 colonies in 0.1 ml of tube with dilution 10^-3
So convert it to ml
10 × 10= 100 × 10^3 cfu per ml
So in 1 ml cfu count is = 100× 10^3
Multiply it by 50
= 100×50 × 10^3
= 5000×10^3
Cfu present in 1 gm of original soil sample
Please watch my video on how to calculate CFU/ml

[@iqrakhursheed7480]

Sir how to make different concentrations of the fungus

[@rbrlifescience]

As per my opinion fungal samples should be blended in specific broth medium before making dilutions. The procedure of serial dilution in similar as described in video.

[@sumitm8904]

Can we use this method for counting the anaerobic bacteria ?

[@rbrlifescience]

You can make dilutions and then do pour plate method to count the anerobic bacteria. There are anaerobic jars in which you have to incubate plates.

[@sumitm8904]

@@rbrlifescience Thank for your explanation. But while performing dilutions there is a possibility that the anaerobic bacteria will come in contact with oxygen and the bacteria will die.So in order to protect anaerobic bacteria to get in contact with oxygen while dilutions, what precautions we have to take?

[@rbrlifescience]

@@sumitm8904 Dear sumit I totally agree with you. Generally any work on anaerobic bacteria should be carried out in anaerobic chamber. This chamber is purged with nitrogen and Carbon dioxide gas so all oxygen is removed from the chamber. Initially everything apparatus such as plate pouring, dilution making is done inside this chamber. and after plating plate is also placed in anaerobic CO2 incubator. It's little bit difficult to work with obligate anaerobic bacteria. There are different types of anaerobic media such as semisolid media which you can inoculate such bacteria.

[@prathamkuril8277]

👋

[@rbrlifescience]

Welcome

[@nithya1141]

Which tubes have present actinomycete, bacteria?

[@rbrlifescience]

It depends on samples

[@aitakil7294]

290× 103 and 32×104 how To calculate them?

[@rbrlifescience]

First convert 290x 10^3 = 29x10^4
Now take average of both
29x10^4
32x10^4
------------------
Answer is 30.5 x 10^4

[@mitalibhoir6380]

Will this dilution technique useful for oil contaminated soil ?

[@rbrlifescience]

Yes, off course but you have to choose correct medium of agar plates for plating as per type of microorganisms

[@mitalibhoir6380]

@@rbrlifescience Yes!! we hv used Bushnell Hass agar as a selective medium for the isolation of hydrocarbon degrading bacteria. We did ur dilution technique. Thank you 💯🙏👍

[@rbrlifescience]

Most welcome mitali @@mitalibhoir6380

[@saurabhchoudhary3435]

Sir , is it true that single colony produce by single bacteria

[@rbrlifescience]

a single bacteria will multiply a number of times and then only we are able to see a single colony. A single bacterial colony is made up of thousands of bacteria that arise by multiple cell division of single cell only.

[@pawar6444]

Hello sir

[@rbrlifescience]

Hello, Good Morning

[@aitakil7294]

How to do of average figures?

[@rbrlifescience]

All dilution must be the same

[@BritishBeachcomber]

That's how homeopathy works. Or doesn't.

[@mishraratnesh6426]

Sir mai aap se kuchh puchna chahta hu plzzz reply dijiye

[@rbrlifescience]

yes bro

[@mishraratnesh6426]

@@rbrlifescience Sir maine BSc (BZC) se ki hai , mujhe ,Media preparation, Autoclave operation, Watter sampling and testing, MLT testing ati hai kya mai microbiologist ban sakta hu plzz btaye ,ya apna no. Share kar dijiye taki me apse kuchh puchh saku

[@rbrlifescience]

@@mishraratnesh6426 Dear Ratnesh if you are a life science student then you can do the job of microbiology. If you have good knowledge of microbiology then there is no problem of getting job in the field in the microbiology. I am postgraduate in Zoology but right now i am working in microbiology field. So cheers.

[@mishraratnesh6426]

Sir ap contact no denge kya ?

[@rbrlifescience]

@@mishraratnesh6426 yes you can call me 9673690563 either on Saturday and Sunday

[@sneha-zb1yg]

32×103 should be divided by 10 not multiplied by it..I guess

[@rbrlifescience]

Dear Sneha initiatilly we calculated how much bacteria we got in 0.1 ml of sample.
for plating we generally take out 0.1 ml of sample.
once you calculate how many cfu in 0.1 ml of sample then we are simply converting it into 1 ml.
multiply (0.1 ml X 10 )= 1 ml
thats why we need to multiply cfu present in 0.1 ml with 10 and we will get cfu/ml.

[@sneha-zb1yg]

@@rbrlifescience thank you so much for clarifying..

[@rbrlifescience]

@@sneha-zb1yg most welcome and please suggest any topic or concept on which video is required.

[@sneha-zb1yg]

@@rbrlifescience if possible please make vedio on plaque assay, and protein-protein interaction techniques..

[@akashrathod6065]

Rahul sir no send kara

[@rbrlifescience]

सुरक्षेच्या कारणास्तव मी तुम्हाला माझा मोबाईल नंबर देऊ शकत नाही जर तुम्हाला काही सहकार्य हवे असल्यास तुम्ही कमेंट करू शकता आणि मी त्याचा रिप्लाय तुम्हाला देईन

[@akashrathod6065]

Ok sir

[@rbrlifescience]

May I know which subject or degree you are studying?