How to Calculate CFU per ml of Bacterial Sample? in 3 Steps || cfu/ml in Microbiology
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- เผยแพร่เมื่อ 19 มิ.ย. 2024
- 7 minute video How to calculate Colony forming Unit per ml of bacterial sample?
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0:00 Introduction
0:15| Correct Equation to Calculate cfu/ml of bacterial sample
0:35 | Example question What is the number of CFU per ml of original stock?
0:48| Step 1: Find out the plate with countable no. of colonies
2:00 |Step 2: Find out the Total Dilution Factor (TDF)
4:48 |Step 3: Substitute the Values in the Cfu/ml Equation
6:38 | Summary of Calculating CFU/ml
Serial Dilution Method Protocol Step Wise Explanation
• Serial Dilution Method...
Dilution and Dilution Factor in Microbiology|| How to Calculate Dilution factor in Serial dilution?
• Dilution and Dilution ...
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I'm a farmer not a trained scientist But I wanted to check my own cfu on the biological products I buy. This video is the best step y step. Thanks a lot for shearing your pedagogical skills
I love how concise and to the point this explanation is. Helped me a bunch!
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A simplified video explaining to calculate cfu/ml of original stock?
Hope this will help
Watch, Understand and enjoy Biology :)
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thank you so much Sir. this is very helpful in doing the research i'm doing right now
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A very awareness explanation by good manners 😊
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Nicely explained. Thank you
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Thank you so much! I'm taking A-level Biology and you explained it so well :)
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thanks. it helps a lot
Good sir.easily understand.thank you sir
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Thank you so much ❤!
Nice explanation
Very well explained
very helpful thanks
Thank you sir.
thanks man, great video!
Thank you for your nice words
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Thank you
Thank you very much greatly helpful
You're welcome!
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Very helpful, thank you for making this video, i have a question, sir
what if we have 2 plate for each dilution (duplo) how to calculate ?
Example:
10^-3 10^-4
Petri 1 287 31
Petri 2 292 42
Vol of stock transferred = 0.1 ml
Thank you, sir
Thank you so much 😭🫶
Your videos are excellent, if possible please make some videos on PCR, Elisa, buffer preparation, sds-page, electrophoresis, endotoxin test and their calculations.
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Thank you so much. I just couldn't figure this out with the materials provided to me by my professors. It's so hard sometimes to figure out these things out for yourself, when the given material is always in a foreign language and has many terms, which you only are familiar with half at the best circumstances..
Glad I could help! Thank you so much for your nice words.
Great
Thank you so much
you explained it very well 1st time I tolerate it ^_^
Please I have a Q, might be silly. now after counting it plate, tube (f) looks has low count, but after calculation it will be 10*8 in compared to tube (e). Does it mean higher count (f)?
.
Welcome.. Thank you for the question. We often wont consider the plate with low count or high count as the chance of error is high. Hope I answered the question.
Thanks a lot
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Welcome Rezvan...Thank you:)
Nice
Sir how to calculate aliquot factor in endophytic analysis??
Thank you sir 🤭 now Im ready for my exam!!! 😂🤗
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hi, If we use raw samples there will be have dilution factor. Also how to count for raw samples transfer into plate 0.1ml.
c'est quoi la différence entre ufc/ml et ufc/g ? quand on doit calculer par ml et quand on doit calculer par gramme ?
What if I want to have a bacterial solution with 1*10^7 for example. How do I have to prepare it with this 6.3*10^7 solution?
Hello sir
Thank
My question is
Sample of E. Colo it is use one loopful cuture on the plate
How do you get ten into the plus numbers? The calculations gives 10 to the (-) values Right?
Hi Sajith
We are using dilution factor not dilution.. Hope this video will help th-cam.com/video/EOsYOfKMgS8/w-d-xo.html
Thank you so much
@@biologyexams4u OK. Thank you so much for understanding me. Got it.
What if all of them are within range, will you find the CFU/ml of each and then find the average?
From the 6.3 x 10^7, how to make it 10^6 bacterial suspension?
What is the formula if we get countable colonies in two plates ?
if you diluted 1ml of a sample with 99ml of sterile water then plated 100ml of the diluted sample on plate count agar and 75 colonies were found. calculate the cfu/ml of the original undiluted sample can you please help with this
First E. Coli sample O.D is what we consider?
how can we get 10^7 as final when we had 10^6 after 10^5/0.1?
alternatively, couldn't you use: no. of colonies/ TDF x Volume of culture in mL? As long as the units are consistent between DF and Volume and multiply those two values and then divide that by the colony value
how is the voulme plated i.e 0.1 ml is calculated?
Thanks times 10 raised to 10000000
Thank you so much. really appreciate your creativity. happy to know the video helped. Pinning this comment. take care, stay blessed
Great presentation. Why we took 0.1 ml and how we can take such minute quantity.
Small quantity; there wont be overcrowding of bacterial cell. We can take small amount using micropipettes. thank you
You can use calibrate your micropipette at 10 microliters (=0.1 ml)
How did you get 443, 63, and 3 colonies?
Hello Sir, I've a question. How to prepare 4×10⁸cfu/ml ? An earliest response will be immensely valuable.
i have a broth culture of a bacteria and i need 10^9 CFU/ml concentration of it to inoculate in a material. Can you help me how i can do that?
What is your colony count? Did you do serial dilution and plated on an agar plate?
Someone give the value of total number of cells
83x 10 power 8.
Is it possible.. please clear calculation if possible
Why is vol. of culture plated 0.1 ml and not 1 ml as you use 1 ml from the total 10 ml?
If 1 ml is used there is always a chance of overcrowded plates
@@biologyexams4u then the 63.10^6 CFU should be in 0.1 mL not 1 mL and afterward express it in CFU/ mL
what is the difference between ufc/ml and ufc/g ?
ML for when we use a liquid sample (eg milk)
G for when we use a solid sample (eg a sandwich)
How do we know the number of colonies?
Using bacterial colony counter...
Thank you
Sir why we don't consider minus sign of dilution when doing calculations ?
Really appreciate your question.. Here we are using dilution factor not dilution.. Hope this video on dilution factor will help th-cam.com/video/EOsYOfKMgS8/w-d-xo.html
Thank you so much 👍
Dilution will be done by adding media or distill water or buffer
Hi Mandeep
Phosphate buffered solution or isotonic saline solution (0.85%)is commonly used as both wont causes osmotic shock..other diluents is Sterile distilled water . Often the selection of diluents depends on the further procedure and also the type of microorganisms ... Like for cell count or for subculturing..
Thank you
not understanding how it went from 10 to the 6th power to 10 to the 7th power though can someone explain?
Hi Sharisa Lee
Which part? Is it the final calculation? 63X10*6 and 6.3x10*7 both values are same.
yes, you were at 10 *6 but final answer became 10*7 so I'm not sure how final answer became 10*7
is it because once you move the decimal over to the left to get 6.3 instead of 63 , the 10*6 changes to 10*7 ?
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Thank you Brother; Take care, Stay blessed... Love from India
Total dilution factor ..? How to calculate that ?
Hi hope this will be helpful in understanding dilution factor th-cam.com/video/EOsYOfKMgS8/w-d-xo.html
Volume of culture plated should be 1 ml
This is the standard procedure as per the manual. There may be change in volume.. Yes... Thank you
Hindi language me video banate to pura samj aata