Lac Operon
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- เผยแพร่เมื่อ 28 ก.ย. 2024
- NDSU Virtual Cell Animations Project animation 'Lac Operon'. For more information please see vcell.ndsu.edu/...
The Lac Operon is an example of an inducible system of gene expression. Its default state is to be inactive. Only when the right catalyst is added to the system, in this case the sugar lactose, is the process activated, allowing the genes in question to be expressed.
i really find that 3D visuals of the molecules really help my brain much much better than simple 2D squares etc. Really good video
surya grahan
And much better than a bunch of words, especially, if not put together in a comprehensible manner !
There's no point complicating simple stuff :)
@@mdsouthcarolina6143 hiruniws
100%✔✔✔
Absolutely yarr... it's made me easy to understand this topic 🤟
That's not true. Lactose does not bind onto the lac repressor. Lactose gets transformed into allolactose, and the allolactose binds onto the lac repressor
In three minutes would you be able to talk about the transformation of beta 1-4 linkage into beta 1-6 linkage? be fair ok//
Emilio Duverna It is possible. Or you could just extend the vid a bit more. take this as constructive criticism (rather than deconstructive criticism). My comment would help people not to get confused. imagine in an exam, if the question is asked 'what binds onto the lac repressor?' and you say 'lactose'. You would lose a mark
Super ... Can you link me to the research paper please!?
ShineyM you are right and I am agree with u
@@emilioduverna4741 yeah in less then 3 minutes I bet
Thank you so much for posting this. I have a test tomorrow on the lac operon and stuff like this, and up until now I've been absolutely lost. I may actually not fail! Thanks!
wow since 13 year
this video is simply amazing thanks for uploading it .It just cleared out all my doubts…
very good
thanks a lot
I understood it very well
How are you after these 12 years?
Hey yo how are you ? My god after hustling bustling 12 years😮
So 12 years ago u was a normal biology student, but now u r a youtuber with 3.76M subs
Great
Here are some notes too.
th-cam.com/video/YSvoid3wjbc_/w-d-xo.html_THE_LAC_OPERON
ig this is your oldest yt comment
The inducer is allolactose rather than lactose itself, but a great visual representation.
Alloactose or lactose can activate the repressor
Both
Thank you. :) We use Autodesk's Maya.
Really easy to understand now, I wish I discovered this video sooner!
glucose decreases adenyl cyclase- reducing cAMP thereby repressing the lac operon. the purpose of the lac operon is to make glucose by breaking down lactose (glucose + galactose). when there's plenty of glucose, there's no need to breakdown lactose to get it.
saw this on mute when in my online class same topic was going in and helped me a lot to understand
Thank you for the teaching of the lac Oberon.
Blessing to you.
There is a CAP activation protein and it is VERY important (although, not necessary for expression of the regulatory genes).
Here's how it works (if you or anyone else aren't sure how it works):
if there are low levels of glucose, then the cell needs another energy source (like lactose). As [glc] is low, [cAMP] should be high, and this is the substrate for CAP to activate it. The active CAP binds to the promotor sequence and facilitates the binding of RNA polymerase.
i have a final for biology tomorrow and my teacher showed this video to us earlier in the year. it helped me so much then and it helped me so much now to refresh my mind on this topic
These three minutes of watching is equal to 30 minutes of reading. Great material, thank you very much!
wow
@@bodydiab9249 Wow indeed, you have replied to a 15 year old comment.
Even though it's missing a few information, it was a good visualization of how the whole thing works.
thmbs up for a video that is very helpful as a review tool
This is really good.
I didnt even undsrstand what an operon is.
But thanks to your visuals bow i got it its a part of DNA and how it works properly
Very simple and perfect explanation to the complex content
This is actually a little broad. When Lactose first enters the Prokaryote some of the lactose is converted to Allolactose. Allolactose is the inducer molecule that binds to the repressor molecule that removes it from the Lac Operation site and allows mRNA polymerase to come in and transcribe the operon
From india , literally ncert choked me up with their description thanks fr this 🙂
What I was taught was that lactose can not enter the cell through diffusion alone and must enter through permease as it is not a small non polar molecule. The question was how could this regulation continue if there was no permease as proteins have turn over rates and dont last forever, and if there is no lactosse within the cell. Apparently there's something called sneak transcription where the represser like all proteins turns over and the polymerase sneaks in and transcribes more mRNA before a new repressor is put in place. Correct me if Im wrong.
Thank you for this video. Reading my notes from class doesn't make ANY sense at all but this all does!
I have this topic almost in every sem... I always watch this video... Its just awesome..! I seriously dont go through the theory.. I will just watch this video😇
The only problem I have with this animation is that the mRNA is not degraded. It shows ribosomes after ribisomes using the same mRNA strand to make many proteins. It's my understanding that mRNA is degraded after one use so that proteins aren't continually made. It should have showned ribonuclease breaking down the mRNA after one set of proteins were made, and then RNA polymerase starting over again. Am I wrong?
you didn't mention that the entire operon is transcribed at a low level due to the occasional failure of the lacI termination mechanism, which is why lactose can enter the cell and how it gets converted to allolactose (by beta-gal).
Thank you for making this video. It's excellent for class instruction.
what i did not understand in class for 30 mins that i have understood in 3 min :P
This is very helpful, the best one by far!!!!
ok.. as it seems like from the video. The rate of lactose entry increases exponentially as permeases are made. THus with more lactose, the chance of the repressor protein binding to the promotor should decrease, but in the video it shows that after a certain amount of lactose is inside the cell, the repressor will start to bind again thus slowing down transcription again. Why is that the case? Shouldn't more lactose mean repressor won't bind again until lactose is once again gone?
good picturisation...................
Just 3 min video and i am clear with the concept ..applause🙋
Superb video 📹
Helped me a lot 😊
Excellent explanation
I find it very useful .. it made my concept very strong. . Thanks for this video. . !!
Thank u for taking time to make this video
thanks your video helps me really well to understand lac operon properly
This is super genius.i watched it once and i don't need to open my book anymore..^.^
thanks even for someone learning this in another language its good explained. very nice Visualisation there should be more videos like this. Teachers should use them at school
"If animations were being played in universities,
we human race could save save so much more time of our short life wasting in studying, and do greater things earlier in life"
I have sooooo much information to study *bangs head against wall* this helped me a lot though! Visualization is great
Ganabb
that was the simplest lac operon video...
Therefore, it's too simple to university students :) It doesn't give me any rellevant information!!!
Sad 15yo high school student noises
lac operon actually came up in the exam! this really helped :)
@Manish kashyap damn are you okay?
so helpful thanks a lot! I watched it after the amebia sister video which was so helpful as well and this one explained everything more!
That was a damn fantastic informative video!
The allolactose binds the repressor protein, not lactose.
what does the released lactose molecule from the repressor now function? or what is the fact of those lactose molecules?
The animation videos are very useful, thank you!
How old and amazing this video is 😍🤩😃
I'm sorry for asking, but isn't allolactose that comes from lactose, the inducer? I really am confused now because I thought that allolactose is the inducer. Please correct me if I am wrong. Thank you
You are right...it's just a simplified video for school children...have a great day 😊
It's so stirring.
OMG that's cool I was just wondering this helped me a lot thank you so much for this verio
I like it .. but it would have been better if you added the CAP region and the adenylyl cyclase ... thank you
Thank you mam.it was very useful for me for understanding the lac operon
I'm here 13 years later 😱
My biology class made me watch this!! Thumbs up if you are too!!
And why did this pop up when I wanted a BL21(DE3) -IPTG induction animation????????????
Thank you sis it is greatly used for me.keep on
it is not lactose that binds to the repressor, it is hallolactose!
Didn’t even mention CAP protein which is made when there is decreased glucose concentrations that promote RNA polymerase binding. Also didn’t mention basal expression of lac operon which is responsible for the production of allolactose made by the expression of lacA which binds onto free and bound repressor proteins that are made by the lacl gene upstream. Many things weren’t mentioned and I’m still a noob to even point it out but even a noob knows when he’s not getting the correct picture.
Very helpful! Thank you! xoxo
@Mrbaltimore108 I am a Lac Operon IRL and this is exactly how it works. Owned.
What about the CAP-glucose relationship?
This is crucial and was left out!!!
guys u should watch shomu's biology videos for lac operon. he has extensively explained this topic
Thank you so much. Excellent job. .....
Very nice
But i want to say that
Your text nad the voice timing is not matched
Plz focused on that for facilating the study. Thanku
Very helpful and well explained! Thank you very much!
simple and valuable. well done
Thank u so much. It's very useful for me
A little dizzy , but great fundamental help. Thanks!
could anybody please tell me the music that is played in the beginning...??
Excellent video
Awesome and very informative video. Thanks!
incredible video. Thanks you so much
i love it. thank you so much :33
great explanation
Thanq sooo much , this is really good video
It is much good ...thankuu so much 🙂it really helped me a lot
Nice explantation super I understand very well
This video is so simple and brilliant :)
@BPat1218 I appreciate your explanation, however the video is clearly only explaining the process not the circumstances by which the process is activated. It would be nice if they added that tidbit of information at the end or beginning. This video definetly helped me. I hate the book it makes no sense.. kinda like your post. It's not that your not explaining it clear, its just too much for me to take in at once. So for beginners this cartoon does it best. Maybe you could make a follow up ?
Thanks! It was very helpful
Oh my god!! that was wonderful!!
thnk u soooo much for clear concept. :)
Great animation. Thank you :D
After 13 years ❤️❣️
Nice explanation
That Lactose molecule looks like a tylenol tablet.
In the year 2020, kindergarten kids will be learning this stuff.
Hiiii, it's 2021 and no😂
Thanks! Nice explaining, it was very helpful!
so beautiful
This was just about the 2 protein molecules... can someone tell me about the 'a' gene that codes for transacetylase.
Good video as well as good explanation
it was simple n clear . thanks
actually it has to b in a simple way so tat we understand witout any complications.... thanks to this video ..
Best one i have ever seen
2 proteins are
β galatocydase
permese
Beautiful!
sampled the keys at the start and made a beat
Very good, thanks :)
Awesome! Really useful.
thanks forlo! that cleared up some of my confusion :)