I am delighted to hear that you found the tutorial informative. If you have any questions or need clarification on anything, please ask. If you enjoyed this tutorial, please check out my other videos for more useful content. Thank you again for watching and supporting!
Hi can you help me understand about what to be inputted in "known distance" and "unit of length?" Because the sample we will be analyzing is the stomach of mice...we will analyze the total area of ulcers produced. I hope you will reply...we are now doing our experimental research.
Hello, thanks for reaching out. If there is a scale image in one of your mice stomach images, that will be used as the measuring unit/length for all the subsequent images. To set the scale, first draw a line parallel to the scale. It does not matter how long it is drawn. In the above video the line is parallel to 1 cm in the scale. In the known distance input "1" since the line drawn equals 1 cm. in the unit of length input "cm" because this is a cm scale. alternatively you can also input it as 10 mm where known distance will be 10 and unit will be in mm. the distance in pixels will be set automatically. All the best with your experiment.
@@nrttaye4033 can we ask help via email? we are not really sure how to use image J software... especially the sample is the stomach of mice 🥺. We already have here the picture of stomach of mice as sample.
It is really helpful.. thank you
I am delighted to hear that you found the tutorial informative. If you have any questions or need clarification on anything, please ask. If you enjoyed this tutorial, please check out my other videos for more useful content. Thank you again for watching and supporting!
Hi can you help me understand about what to be inputted in "known distance" and "unit of length?" Because the sample we will be analyzing is the stomach of mice...we will analyze the total area of ulcers produced. I hope you will reply...we are now doing our experimental research.
Hello, thanks for reaching out. If there is a scale image in one of your mice stomach images, that will be used as the measuring unit/length for all the subsequent images. To set the scale, first draw a line parallel to the scale. It does not matter how long it is drawn. In the above video the line is parallel to 1 cm in the scale. In the known distance input "1" since the line drawn equals 1 cm. in the unit of length input "cm" because this is a cm scale. alternatively you can also input it as 10 mm where known distance will be 10 and unit will be in mm. the distance in pixels will be set automatically. All the best with your experiment.
@@nrttaye4033 can we ask help via email? we are not really sure how to use image J software... especially the sample is the stomach of mice 🥺. We already have here the picture of stomach of mice as sample.
Could you send me a sample image that contains a scale/measurement unit .
Happy to help.
@@nrttaye4033 ofcourse! Thank you so much
@@nrttaye4033 can I send it through email?