Managing monokaryons for crossing/breeding/mating experiments - workflow, labelling, and sanity.
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- เผยแพร่เมื่อ 1 มี.ค. 2023
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I've learned more in 3 weeks watching your videos than I have in the year I've been growing. Your truly an artist in your craft I appreciate all the information you have given. It's truly saved me in so many ways. Main one my reduction in cross contamination. Two keeping it's simple but making sure you stay labeled up and organized. Thank you very much
Thank you very much for taking the time to write this wonderful comment. You made my day!
You explain so simple just like a Dr.!! Lol thanks for all your work and everything you’re doing for our community my friend!!
🎉 thank You Sir
Wow hands down the best myco. on TH-cam 👏
The edding 750 paint marker inks are the best choice whenever an alcohol-resistant marker is required.
I have my "7/11" on it's way due to your recommendation. Excellent content sir! Thank you for sharing your knowledge. It is very much appreciated ✌️ Much love from Scotland
Brilliant conversation definitely interested in this breeding technique
No Ed, I am totally down with SBI
Single Basidiospore Isolate just makes sense,
More of us are leaning into it than you think.
I’m always interested in what ya doing Ed!!! I love the idea of all of this and want to learn more and more!!
Thanks for all this information dude, I'm not quite on this level yet but its cool to see it. I just appreciate you sharing the journey with us.
Another great video on this topic. Thanks for the time and care you are putting into these videos. They are so helpful,clear and thought provoking.
If I order from your store, about how long do the orders take to reach the US? I want to support the time that you spend on this endeavor. It really has given me something to look forward to and got my lazy retired butt back into something that keeps my brain functioning.
✌️
Usually about 30 days to U.S. for swabs. 10-12 days for LC (different shipping method).
I'm glad it has sparked something in your brain. Same for me. It keeps the Alz. from creeping up too fast.
I get my order marked sent from spore swaps just then while watching your video. Thank you for the freedom of great information.
Killin it Dr. Much thanks Ed Grand
A lot of info. I understand about 10% of what you're talking about, but I figure I will learn by osmosis eventually. You are the master Ed! Thanks!
Thanks so much Ed for your mission.
This was a fascinating video on a very interesting topic.
Looking forward to having it in written format (although with your brilliant videos I can think of taking the subtitles / transcript and adding some pics) at some point.
Ohhhh. I forgot about the transcript thing. THANKS!!!
@@edwardgrand BTW there is a way better transcript making tool, outside of TH-cam (meaning one needs to download the video and run it) called Whisper. It also creates the timestamps but is using ai and does construct real phrases, very accurately.
I'm Glad I went through a few pages of fresh from the farm fungi, when I searched HYBRIDIZATION, ive watched all his vids. I found your channel greats videos lucky I found you. Both great channels tbh plenty of information to absorb. Ty
Silver sharpies are the bomb kid! They do not wipe off when cleaning your plates.. 😜🤘
Awesome video I'm excited to try some of these lab techniques and cultures hopefully sometime, some very informative videos, thanks for sharing the knowledge. Mushluv 🍄
Your channel is on point for no BS to the point info. great job
Thank you as always Ed!
Thank you sir🙏🏽🙏🏽
I’m definitely interested in your genetics Ed! Been a big fan since I seen u on sages channel! I know Brock Lee too, he’s the man! & I’d feel bad for not paying u, but if u insist! Lol 😈 I could at least cover shipping, anyhow I look forward to talking with u man, & getting some of those amazing monos 🤤
So glad to have been introduced to your work and had my attention brought to you through the Mycogeeky podcast. I am so fascinated with the mating of monokarions and ending up with something completely new and I really appreciate you sharing this process with the world. I am fairly new to mycology, however I have really found this process incredibly fascinating and just wanted to thank you for sharing your knowledge. If I wanted to contact you away from youtube how might one do that? Keep the knowledge flowing and I will keep watching!!
I am on FB, IG and a bunch of Discords. FB is probably the easiest.
@@edwardgrand well, I certainly appreciate the Content. Warren really cool thing about getting into the mycology community is getting to meet people actually educated in this discipline. Seems like a large cross-section of people I have come across come from an educational background. The funny thing is I’ve never been into science in my life and I guess it took mycology to make it accessible to me. I have my masters in behavioral sciences, which is the closest to science. I’ve ever managed to find it. Interesting until now. Anyway, you have a good day!
I'm definitely interested in your crosses the only thing is I live in canada 🇨🇦
7:28 😂 your a madman Ed lol
I need to clone myself...
Dear Santa, I want a microscope. 🤣
Ask the Easter bunny to put it in a big egg ;)
I watch almost all of your videos Dr. Grand, but this is advanced mycology. All I do with my agar is germinate my spores, clean them with transfer, then put to grains when my plates start to look clean and good. I don't even have a clue what's a monokaryon or a dikaryon lol. But I love watching you work and explain this stuff.
I'm wondering if my agar work is as simple as what I said above, what benefit would I get if I were to learn what mono's and di's are?
Breeding and general knowledge. But you don't need to know it to grow perfectly fine.
This really interests me and I want to learn how to do it. It seems kind of like genetic editing.
Would love to see how to pour plates. Temp, ect. I love how you have zero condensation even with plastic wrap! :)
I have a video on my channel somewhere. You just have to pour when it is a few degrees from solidifying. Takes some practice. Depends on recipe a bit.
@@edwardgrand what temperature is that for you?
@@nickfranco2259 I know from my hand. Never measured the actual temperature.
@@edwardgrand Great thank you so much!
@@edwardgrand Been watching your videos. 👌
Love your stuff ED!
Thanks for the videos. I would gladly take some mono's off your hands in the name of science. I am currently in the process of isolating and breeding. Hmu.
I love the no.7 handle. I have 10 😅
Only 3, but need another for side-pin surgery
I only have 6...might need to order a few backups for the #12 blades :) Didn't buy myself a present for a while. You should get some fancy tweezers.
Search "Stainless Steel Diamond Jewelry Tweezers Gemstone Pincette with Adjustable Antislip Lock Jewelry Tweezer Hand Tools". Something like that. They ROCK!
@@edwardgrand I will have a search on Amazon. I bought some that have a lock, but they are a bit flimsy. I will look for some that are better quality
1. Thank you, thank you, THANK YOU for your amazing channel. Your work and vibe is such a gift :)
2. Any thoughts about horizontal vs vertical laminar flow set ups?
I've used both extensively. Don't really have a preference. TBH, the cheaper FFU things they've got out now are great. Just get one that's high enough, especially if you are gonna work with bags.
@@edwardgrand Okay, thanks so much. I'm in Africa and there's a vertical one available here (where it's quite difficult to get or build without huge expense) - so I might grab it! Thanks again!
I wish that public libraries had microscopes.
Some do!
Check the local high school science lab. They should have one laying around. Only needs to be be a basic one with 400X and maybe 1000X if you want to see spore ornamentation.
Another awesome video! I'm inspired to take the next step with my hobby. Can you please recommend specifications (or even a good model/brand) for a proper microscope to conduct this work effectively??
Amscope B120C is more than enough scope for this and other Mycology work. Have a beautiful time!
The sweet B120C has a one-eyed brother, M620
I just got mine in, 10x eyepiece option for sub $200.
Same compound scope from the turret down!
They surprised me by including a 25x B120C eyepiece pair.
All the cool 120 accessories are compatible- from groovy chromatics for the condenser, to the cameras.
EDIT EDIT THEY’RE STILL ON SALE 40% OFF
@@sminstudios Thanks for the info. I have to say, if you're gonna go for it, spend the extra $60 for the binocular version. It is really easy to bash your eye on a monocular one when you're in a hurry. With the binocular version, it's a little easier to avoid the turret. Or at least it cuts the pain in half and gives you matching black eyes!
Hey Edward what do you think colonizes faster a bag or a jar of grain?
Neither. It depends on many variables. Temperature, cultigen, grain hydration, air exchange, etc. I never worry about colonizing my spawn faster. I just keep it clean and wait for it to be ready. If I get impatient, I start another project while I'm waiting.
Do you use parafilm to wrap your plates? Thanks for the videos, helping me out a lot!
Ed uses cling wrap but is trialing grafting tape
(I 💚 grafting tape)
Tossing pens!!!!!
Hey Edward when I make the culture slants you said I need to sterilize the water but do I need nutrients and agar also?
slants or water storage?
@@edwardgrand I’m not sure! Lol I want some slant’s but can I use just water in the slants?
@@Houseofmycology slants or test tubes? Not the same.
@@edwardgrand ahhhh idk! I’m still learning. What’s the difference between the two? I want to take pieces of agar and store them for a long time!
@@HouseofmycologyUse long-term water storage. I have another video about it. Slants are an old-school way to store cultures on agar.
Now, do monos have clamps or do not?
No, they don't have. But can be very hard to find clamps or lack there of under microscope as Ed shows in one of his videos.
Always wanted to know and I've never had the effort to test, but could you not just grow a mono out to coir, then when it's like 90% shoot a few spores in and make a cross that way?
I have heard of that technique, but never tried. It would probably work, but if you are gonna go through the effort (and have the skill) to do all that, it's probably kind of pointless. I guess if you had a pre-existing mono culture and a MS tube, it might be interesting? Lots of options. It just depends on what you have to start with and your skill level. Man, you got me thinking now!
Found this by searching your comment: "If you have a microscope and have agar, please dont be afraid of monokaryon isolation and manipulation. Its fun! When you get one just grow it on agar, then use some mycelium water to inoculate a jar of grain. At 100% colonization shake it and inject some spores for it to mate with. Give them 10 days to have their fun before casing the grain or spawning to a minimal amount of substrate. The first generation will be a nice mix of the dominant traits of the two varieties as homogeneous in character as the variety that the spores came from. If you then grow spores from those hybrids, that next generation will be an explosion of diverse traits for you to select from. With a short list of traits your selecting for, and a good number of small grows in each generation, you can breed a new stable variety in as few as 8 generations."
Might be FUN!
@Edward Grand jees, sounds like I'm going to need to get back on that grain prep, thanks!
@Edward Grand yep, got a mono of averies on agar I'm doing nothing with as it's mainly a hobby for ne and that project had to take a back seat, might grow it out on grain now, thanks for being a catalyst for science!
Do both Mono want to hook together or does a mono go with a mono
It's slightly more complicated, but basically, yes.
Once my agar is fully colonized how long can I keep it before it goes bad?
It depends on how you store it and the recipe. Usually months.
@@edwardgrand ok awesome thanks man
After you have put Some tissue on a plate do you store them upside down or right side up?
Right/normal side up.
@@edwardgrand ok cool I’ve heard people say upside down so the moisture doesn’t sit on the mycelium but I trust you more than them! 😂 thanks man! Oh yeah I have a couple tiny spots really small spots either blue or green I can’t tell but can I get brushing on mycelium on agar?
@@HouseofmycologyYou can store them upside down, but the agar piece is more like to fall off. Bruising of mycelium is common. Just make sure it is the mycelium that is bruising, not some random part of the agar plate.
@@edwardgrand I wish I could show you!
@@HouseofmycologyI am very easy to find on social media.
Any books you would recommend about this?
Anything by Miles, P.G. or Chang. S.T.
Also Radical Mycology. Peter McKoy.
A few of the P. Stamets books.
How can i get in contact with you Ed?
FB and IG
Eeek his hands are naked!😅
I also wasn't wearing any pants. ;)
@@edwardgrand Man I love your sense of humor, full stop but I also reckon in this instance you are telling the truth and your still as funny as heck! You may have missed your vocation in life as a stand up but you are still a stand up kind a guy!I Is it possible you developed your sense of humor, at an early stage in your life, when there wasn't "mushroom"?. bah dum tish! 😅
@@bobhope5773 Next career...Fun Guy Comedian!!! aaaayoooooo!!!
@@edwardgrandLMAO you certainly have the skills 🤣 Who knows, maybe you could also turn your hand to growing bonsai, they use pet tree dishes too! Wakka Wakka Ahhhh! 😆