Picking single colonies ('monokaryons'), get to spawn faster, methodology and a rant on Bro Science.
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- เผยแพร่เมื่อ 28 ก.ย. 2024
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Ed1K, the giant killer! Thanks for spreading knowledge and truth. You are a real teacher. Now I'm going to watch this vid another 12 times. Have a great day! ☮️💜🌱🍄
It always brings a smile to my face hearing your trademark "Helllooo Everyyboddyy!" at the beginning of the videos!
Dr Ed, telling it like it is! If Dr Ed says Sterilize your substrate! Then dag-nabbit, sterilizing I will do! Thanks Ed!
Yes, thanks, Edward. I really enjoy your take on mycology and is something I feel strongly about since starting in mycology. Love your vids
Well I always wanted a microscope 🔬 lol guess it's time to invest. Tnx Ed, always learn something new from you, take care! Peace ✌️ from Welland Ontario Canada 🇨🇦
Amscope b120C FTW! Like $250 US
Super cool video. I do have an off topic question though. What do you do before you sit down in front of a flow hood? Bathe? Clean clothes?
Nothing. The whole idea of the laminar flow hood is to blow stuff away from the work area. SAB is a different story. You should clean and alcohol everything before it goes in the box. But with a laminar flow hood, the technique is what is important. Never work 'in front' or over plates, etc. If you watch my videos closely, you will see how to do it. I hate to say it, but it is something that comes with experience. I cringe sometimes when I see what others do in their videos. Wear tight-fitting gloves, lots alcohol on all surfaces, keep clean stuff upwind of you, etc. Biggest thing! Don't get discouraged. Contamination happens all the time, but if you consistently get it, something is wrong.
@@edwardgrandthanks mate. I go ok at flow hood work. The thing im having trouble with soo far is finding quality agar. My plates are a mix between lc and agar..
@@Matty_76 Telephone brand all the way! 1.5-2%.
Hey Ed, I have heard you say something before about a mono being able to be "sudo rhizo" . If possible could you display a mono that shows those characteristics? I'm pretty sure I have some cultures that might be doing that.
I don't have any images handy. Pseudo-rhizomorphic is just rhizomorphic-looking growth that doesn't have clamp connections. Rhizomorphic growth almost always indicates a dikaryon in our babies, but very rarely, it can be monokaryotic. You need a scope to be certain.
@edwardgrand Thanks for the reply! I have a Amscope I have been messing with. Was just a tad confused when I thought I had a mono verified by lack of clamps start growing rhizomorphic. I even took another slide of it after it changed, and it still seems to lack clamp connections. Guess I'll try to match it with some others and see what we get! Thanks again Ed!!
@Snufulufugus it's hard to know without a photo. It really depends on media too. But if you don't see thick, heavy rhizo, and no clamps, should be mono.
I thought an axiom of science was that you cannot prove a negative. I'm not saying you can't be fairly certain you don't have a clamp connection.
i wish i was smart enough to follow along :/
It's not about being smart. It's about learning, persistence, patience, and practice.
I try to sterilize always, cause when I don't... I get contamination.
Ed's not a Teacher.He's and EDucator🤯
Awe man Ed, I just got my hands on some star frost, lucid gates, starry night ape, shatki, & some other random cultures & spores, can’t wait to follow your videos & cross, & back cross the hell out of this stuff haha!
So diakaryons are rizomorphic? Sorry I’m not good at spelling these big words😂
Cubenis dikaryons are rhizo on the right media at the right concentrations.
Fugggg I'm on break but I got just enough time to watch. 😅
Well … I could not have clicked the Video any faster
i have been sterilizing sub in my clav from the beginning and finally someone speaking the truth. didn't even bothered to discuss with people saying other. thanks for your content Ed ! mush love
Hello sir thank you for the continued education
8:50 This is why I'm focusing on building a grain spawn business. Everyones focused on selling genetics right now. In the meantime, I'm working with all these genetic slingers who can't be bothered to process lots of grain at a time :) :)
Other than sterility, proper grain prep is the most important thing in this hobby. It took me years of experience to figure this out and I still F it up frequently. So many variables in a new batch of grain. Now I know what all those 'food scientists' get paid for! TBH, most people can't cook pasta these days, so you might have a large market. Good luck.
@@edwardgrand I have materials testing experience so thats a huge plus. One of the easiest tests to run is a gravimetric moisture analysis and, as you know, getting the moisture levels right is definitely a huge factor. Having good suppliers and buying large batches is pretty key too, I've found. Thanks man! Take care out there. Lovin' your videos.
So you take monokayon and a dia from a different strain and let them grow together for a new kind of fruit?
You can do mono-mon or dikaryon-monokaryon matings
@@edwardgrand ok it’s a lot to take in 😂 I’m getting it though!
I love ya Ed!!! Yes a new wave is coming! Love your videos
Based knowledge right here.
🎉😊
I'm curious about your story man I've seen you on mycogeeky podcast but why did you move to Bangkok? Might be a good video
I was actually gonna make a video yesterday about that, but the place I was sitting had some copyrighted music playing in the background. Coming soon ;)
@@edwardgrand Awesome cant wait to here the story man!
What cool color plates! How do you get those colors?
I usually just grab random food dyes off the internet.
Thank you as always Ed!!
Ed is the man
Another bomb ass instructional video presentation
Eff the gate keepers
Love the title :) So appreciate you teaching and upholding proper scientific technique, clarifying the correct lexicon and telling people when something is unnecessary to buy for home mycology - you're the best!
Ed, I wouldn't worry about video length. Pretty sure TH-cam likes videos over 10 mins because they can stuff more advertising in the middle
Are they putting ads in my stuff already?
@@edwardgrand No I don't think I have noticed any. Chances are, they will soon. I have 1.3k subs, but I am not monetised. I have been told that sometimes my videos have ads
Ed another 5 grams grand 🫶🔥🔥🔥
100 Grand at Least. Without the 13 minutes we might not get the silky smooth explanation that makes any and all gatekeepers shudder. Keep em running for the hills. Straight up top notch. Praises