Thank you for doing these videos. I have a background in bacterial isolation and DNA sequencing. Watching this is very familiar regarding isolation. I appreciate the very concise and logical examples you provide. Thanks for always dropping gold nuggets 🙌🏼
Sir, you are the best - no idea where you get that positive energy from... As small thank you I will invent magnetic mycelium for you. Then you can push it around via induction.
This was an awesome breakdown of the complexity of mating techniques. I feel like I can absorb this into my feeble brain. Thanks Ed, now where did I put that lions mane.....?
Thanks bro. I love to see your place coming along. I have a question, what type of fule do you use in your alcohol burner. A farmer down the road from me uses a product called 'Heat'. He claims it burn hotter than anything. I'll been using 70% rubbing alcohol but I've noticed it takes a while to heat the blade.
95% denatured alcohol. It's the safest and cheap. If it spills, it evaporates faster and burns at a lower temperature. This is a good thing for safety. Anything over a few hundred Celsius is gonna annihilate contamination and residues.
If you left a plate of monokaryons to grow out on a plate. Let them do their thing. Would that plate end up as one or would you be able to pick off different phenotypes from different areas of the plate
You can probably pick off as many dikaryons/genotypes from a plate as you want. It would probably be better to fruit a MSS or from a T0 plate if you are looking for distinct phenos.
Thank you for your vids. I love them. So may i ask when making a new mushroom would that be called a strain or do you call mushrooms something else? Thank you sir.❤️have a great day. Also im very excited to watch you make a new type of mushroom
I haven't really given any of them names. I like the term 'cultigen'. To keep things organized, I won't really give them names until they hit F4 or F5. I've got dozens of crosses at this point, so it would be harder for me to remember their 'nicknames' instead of the actual cross parents.
More like 4. Ignore the labels. Sometimes I write the next day or the previous day, depending on the time. The spores were hours old. If it were 2 days, I'd be suspicious they were clones. I've checked already. All monos.
@@edwardgrand that’s awesome they turned out I’ve watching only your videos for a couple days now I’m ready to mess with genetics a bit still need a microscope tho. And I did notice you don’t have many follow ups to your methods you have a channel full of cliff hangers😂 love it tho. Much love appreciation and respect to you.
![🔴 ฟุตบอลแชมป์กีฬา 7HD แชมเปียน คัพ 2024 [รอบรองชนะเลิศ]](http://i.ytimg.com/vi/zPm8ClLVhoE/mqdefault.jpg)
🎉thank you
Awesome video Ed! You got me really excited to give this a try!
Thank you for doing these videos. I have a background in bacterial isolation and DNA sequencing. Watching this is very familiar regarding isolation. I appreciate the very concise and logical examples you provide. Thanks for always dropping gold nuggets 🙌🏼
educate 🎶 mee,.. teachh mee 🎶,.. showw mee the wayy edwardd granddd 🎶 🕺 🕺
🍄💙
Just keep watching ;)
Thanks for all you do!
Thank u for this man u have helped me again a great deal . An reaffirmed couple things I thought I knew lol mush love
Sir, you are the best - no idea where you get that positive energy from...
As small thank you I will invent magnetic mycelium for you. Then you can push it around via induction.
Keep this great info coming
Every video is genius @ work
(Literally and figuratively)!! Or something like that! Idk I’m not the genius! Lol❤❤❤🍄🍄🍄🤓🤓
This was an awesome breakdown of the complexity of mating techniques. I feel like I can absorb this into my feeble brain. Thanks Ed, now where did I put that lions mane.....?
Thanks bro. I love to see your place coming along. I have a question, what type of fule do you use in your alcohol burner. A farmer down the road from me uses a product called 'Heat'. He claims it burn hotter than anything. I'll been using 70% rubbing alcohol but I've noticed it takes a while to heat the blade.
95% denatured alcohol. It's the safest and cheap. If it spills, it evaporates faster and burns at a lower temperature. This is a good thing for safety. Anything over a few hundred Celsius is gonna annihilate contamination and residues.
If you left a plate of monokaryons to grow out on a plate. Let them do their thing. Would that plate end up as one or would you be able to pick off different phenotypes from different areas of the plate
You can probably pick off as many dikaryons/genotypes from a plate as you want. It would probably be better to fruit a MSS or from a T0 plate if you are looking for distinct phenos.
Thank you for your vids. I love them. So may i ask when making a new mushroom would that be called a strain or do you call mushrooms something else? Thank you sir.❤️have a great day. Also im very excited to watch you make a new type of mushroom
I haven't really given any of them names. I like the term 'cultigen'. To keep things organized, I won't really give them names until they hit F4 or F5. I've got dozens of crosses at this point, so it would be harder for me to remember their 'nicknames' instead of the actual cross parents.
what is tween
Polysorbate 20, 80. It is a surfactant. You can also use a VERY small amount of dish soap.
@@edwardgrand is the surfactant added to push the spores apart to form more isolated colonies?
Wait your plates showed in 2 days?!?
More like 4. Ignore the labels. Sometimes I write the next day or the previous day, depending on the time. The spores were hours old. If it were 2 days, I'd be suspicious they were clones. I've checked already. All monos.
@@edwardgrand that’s awesome they turned out I’ve watching only your videos for a couple days now I’m ready to mess with genetics a bit still need a microscope tho. And I did notice you don’t have many follow ups to your methods you have a channel full of cliff hangers😂 love it tho. Much love appreciation and respect to you.
Lol another 10 years of work for yourself 😅