Usually the MIC protocol requires to adjust the bacterial conc to 10 to power 5 ufc/ml via McFarland standard comparison of solution turbidity (you have to make an internal standard) and usually the inoculum size (v/v) is 10 % of the final solution volume. So when using a 96 well plate, the final volume in each well should be 100 microliters to which you must add 10 microliters of 10 to power 5 ufc/ml dilution.
Can the 96 well plate be kept in orbital shaker incubator ? Can we get the results if they are being kept at the orbital shaker incubator ?? Can I plz get a reply ??
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How can you be confused this method in microplates can only be used with broth media, agar will solidify as you make your dilutions and add your bacterial inoculum. So all the MIC assay is realized with broth. In addition for a easier evaluation of the MIC results after incubation, an cell oxidation activity reactive should be added i.e.: resazurin which is a blue dye, and wich after a further 2h of incubation will change color from blue to pink if there are active viable cells
Thanks for useful video
How do you determine what concentrations of the antibiotic to use?
how much 0.5 McFarland adjusted bacterial broth needs to added in how much antimicrobial solution?
Colony inoculated in sterile saline and read in turbidometer.
Usually the MIC protocol requires to adjust the bacterial conc to 10 to power 5 ufc/ml via McFarland standard comparison of solution turbidity (you have to make an internal standard) and usually the inoculum size (v/v) is 10 % of the final solution volume. So when using a 96 well plate, the final volume in each well should be 100 microliters to which you must add 10 microliters of 10 to power 5 ufc/ml dilution.
Can the 96 well plate be kept in orbital shaker incubator ?
Can we get the results if they are being kept at the orbital shaker incubator ??
Can I plz get a reply ??
thank you for your comment. I am not sure what you mean. Please feel free to reach out to info@emerypharma.com for your question.
be sure to subscribe to our channel www.emerypharma.com
How much ml every well from the plate???????????
Can anyone attach the pdf of the book she referred to? The "M100S" titled book
it will be difficult to attach the pdf of a book. Thank you for watching. be sure to subscribe to our channel www.emerypharma.com
Thank you
You're welcome
Am confused here which of the medium was this performed was it broth medium or agar medium.
How can you be confused this method in microplates can only be used with broth media, agar will solidify as you make your dilutions and add your bacterial inoculum. So all the MIC assay is realized with broth. In addition for a easier evaluation of the MIC results after incubation, an cell oxidation activity reactive should be added i.e.: resazurin which is a blue dye, and wich after a further 2h of incubation will change color from blue to pink if there are active viable cells
Thank you for watching. be sure to subscribe to our channel www.emerypharma.com
Good
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Job required for microbiology fresher
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Turbidity was not clearly visible
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in 2 ways. microdilution and microdilution. im ded XD
she said macro and micro lmao 😂
@@hafsashereen006 loll I had to repeat it 5 times
I hate microbiology.
sorry to hear! studying microbes is very important to our health. Thank you for watching. be sure to subscribe to our channel www.emerypharma.com